##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937774_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2415437 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.531180900184935 31.0 31.0 34.0 30.0 34.0 2 31.654829333160002 33.0 31.0 34.0 30.0 34.0 3 31.692628290450134 33.0 31.0 34.0 30.0 34.0 4 35.29149342334327 37.0 35.0 37.0 33.0 37.0 5 35.27002691438444 37.0 35.0 37.0 33.0 37.0 6 35.34864084635617 37.0 35.0 37.0 33.0 37.0 7 35.280471401241265 37.0 35.0 37.0 33.0 37.0 8 35.26172158495543 37.0 35.0 37.0 33.0 37.0 9 36.8441143362464 39.0 37.0 39.0 33.0 39.0 10-11 36.723873568219744 39.0 37.0 39.0 32.0 39.0 12-13 36.587292278788475 39.0 37.0 39.0 32.0 39.0 14-15 37.694236281053904 40.0 37.5 41.0 32.0 41.0 16-17 37.621268946364566 40.0 37.0 41.0 32.0 41.0 18-19 37.569187480360696 40.0 37.0 41.0 32.0 41.0 20-21 37.390796158210705 40.0 37.0 41.0 32.0 41.0 22-23 37.24554604404917 39.5 37.0 41.0 31.5 41.0 24-25 37.09051322804113 39.0 36.5 41.0 31.0 41.0 26-27 37.19347575614682 39.5 37.0 41.0 31.0 41.0 28-29 37.13278570295975 39.5 37.0 41.0 31.0 41.0 30-31 36.926744518693724 39.0 36.0 41.0 30.5 41.0 32-33 36.68489056017607 39.0 36.0 41.0 30.0 41.0 34-35 36.40924106072731 39.0 35.0 40.0 30.0 41.0 36-37 36.42489516389788 39.0 35.0 41.0 30.0 41.0 38-39 36.37651903154585 39.0 35.0 41.0 30.0 41.0 40-41 36.17511448238973 39.0 35.0 41.0 29.5 41.0 42-43 35.91657555133916 39.0 35.0 40.5 28.5 41.0 44-45 35.67954701364597 39.0 35.0 40.0 27.5 41.0 46-47 35.30517107256368 39.0 35.0 40.0 26.5 41.0 48-49 34.9573393965564 38.0 34.5 40.0 24.5 41.0 50-51 34.060328627904596 37.5 33.5 39.5 23.5 40.5 52-53 34.16505812405788 38.0 33.5 39.5 24.0 40.5 54-55 34.45488476826347 38.0 34.0 40.0 23.5 41.0 56-57 34.179051865149034 38.0 34.0 40.0 22.5 41.0 58-59 33.935915943988604 38.0 33.5 40.0 22.0 41.0 60-61 33.62435223936704 37.5 33.0 40.0 20.5 41.0 62-63 33.24319843572819 37.0 32.5 40.0 19.0 41.0 64-65 32.85641418095359 36.5 32.0 39.5 18.0 41.0 66-67 32.43090442847402 36.0 31.5 39.0 14.5 41.0 68-69 31.936397016357702 35.5 31.0 39.0 10.0 40.0 70-71 31.479228603354173 35.0 31.0 38.0 8.0 40.0 72-73 30.999023779133964 35.0 30.0 37.5 7.0 40.0 74-75 30.489485753509612 35.0 30.0 37.0 2.0 39.0 76-77 30.018632860223637 34.0 29.0 36.5 2.0 39.0 78-79 29.469511107099876 34.0 29.0 36.0 2.0 38.5 80-81 28.9258602066624 34.0 28.5 35.5 2.0 37.0 82-83 28.38844378884649 34.0 27.5 35.0 2.0 37.0 84-85 27.892441202150998 33.5 26.5 35.0 2.0 36.0 86-87 27.349857810408636 33.0 26.0 35.0 2.0 36.0 88-89 26.835555429514407 33.0 25.0 35.0 2.0 35.5 90-91 26.290905331002214 32.0 24.5 35.0 2.0 35.0 92-93 25.698923424622542 32.0 22.0 34.5 2.0 35.0 94-95 25.03228525521469 32.0 19.0 34.0 2.0 35.0 96-97 24.21199310932142 31.0 8.5 34.0 2.0 35.0 98-99 23.218001338888158 31.0 2.0 34.0 2.0 35.0 100-101 20.670103587880785 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22018259233837867 1101 2 0.26986721190478846 1101 3 0.2888532061506126 1101 4 0.23155733431970305 1101 5 0.2109006093003316 1101 6 0.2813815051676656 1101 7 0.2921508336822498 1101 8 0.1995095328780252 1101 9 0.33957344606914575 1101 10-11 0.2391687869553465 1101 12-13 0.3780545007155638 1101 14-15 0.565936113121964 1101 16-17 0.5705596615054063 1101 18-19 0.47577691654556276 1101 20-21 0.581838925744627 1101 22-23 0.6670861318516188 1101 24-25 0.5213537701673516 1101 26-27 0.6016414493794358 1101 28-29 0.514941324831014 1101 30-31 0.6301792904722063 1101 32-33 0.5813486856843539 1101 34-35 0.4773275569405513 1101 36-37 0.6099229394016419 1101 38-39 0.4935482488829095 1101 40-41 0.5093815394310965 1101 42-43 0.5114876852299517 1101 44-45 0.5945369563395815 1101 46-47 0.5969406275632352 1101 48-49 0.7140430622169305 1101 50-51 0.566043424894751 1101 52-53 0.5887868798034432 1101 54-55 0.8419751921738126 1101 56-57 0.9759620625748013 1101 58-59 0.7762746947331749 1101 60-61 0.7211630753165323 1101 62-63 0.7231116482528321 1101 64-65 0.7437431945942876 1101 66-67 0.8868466015168224 1101 68-69 1.0361026084943106 1101 70-71 1.0066830144013004 1101 72-73 1.0017400752817416 1101 74-75 0.927889855249326 1101 76-77 0.9163525053656265 1101 78-79 1.162714434304334 1101 80-81 1.2135771093659642 1101 82-83 1.207622991819175 1101 84-85 1.1886620665013403 1101 86-87 1.1328958517961354 1101 88-89 1.1767520653094437 1101 90-91 1.241772822617886 1101 92-93 1.5085279902477957 1101 94-95 1.6155222624691064 1101 96-97 1.7267473499759198 1101 98-99 2.0230827806975356 1101 100-101 2.1870011872021102 1102 1 0.14074544266620137 1102 2 0.1414609731916947 1102 3 0.18021867699147265 1102 4 0.09174128777634394 1102 5 0.0903903253345959 1102 6 0.09174907421045475 1102 7 0.14354596378989015 1102 8 0.06709389769852692 1102 9 0.0805782990941708 1102 10-11 0.13936930916168677 1102 12-13 0.11151665699454583 1102 14-15 0.1620542431214318 1102 16-17 0.16820672094520717 1102 18-19 0.17699620994574872 1102 20-21 0.1536614174949591 1102 22-23 0.278936847573938 1102 24-25 0.2937539942619196 1102 26-27 0.355199173242859 1102 28-29 0.2870014521584352 1102 30-31 0.21518076112553075 1102 32-33 0.2570514890403217 1102 34-35 0.3743033803427238 1102 36-37 0.4710509003846681 1102 38-39 0.42691639073404275 1102 40-41 0.4967184678111991 1102 42-43 0.5405662207368636 1102 44-45 0.3166622481457537 1102 46-47 0.26254215096626154 1102 48-49 0.32874983122954404 1102 50-51 0.3109844036348406 1102 52-53 0.32087417820410025 1102 54-55 0.2937928306371944 1102 56-57 0.41604983294649145 1102 58-59 0.4692098751966114 1102 60-61 0.3997468138980125 1102 62-63 0.3788180188925665 1102 64-65 0.3398566756006005 1102 66-67 0.3511710137596822 1102 68-69 0.25342876430603667 1102 70-71 0.38928827611866623 1102 72-73 0.3238988660301665 1102 74-75 0.21292119732067505 1102 76-77 0.4089873215837265 1102 78-79 0.45980382409713627 1102 80-81 0.5577200065818353 1102 82-83 0.47974263935477524 1102 84-85 0.6708026646263967 1102 86-87 0.6844920148445759 1102 88-89 0.5843928099759985 1102 90-91 0.7736536533392702 1102 92-93 0.9893764956685267 1102 94-95 1.00495172231372 1102 96-97 1.2107223360354915 1102 98-99 1.3699339456042665 1102 100-101 1.618919301539055 1103 1 0.16224788276601032 1103 2 0.26303506303947444 1103 3 0.11320166699613665 1103 4 0.12584172435546037 1103 5 0.1663710695344136 1103 6 0.20459380859420406 1103 7 0.24469486499340576 1103 8 0.24343662065617622 1103 9 0.23101437716400852 1103 10-11 0.3046271981496744 1103 12-13 0.2956377200162592 1103 14-15 0.40435064259632725 1103 16-17 0.3442553570390885 1103 18-19 0.39756789922078184 1103 20-21 0.4323396884145083 1103 22-23 0.4023812352330296 1103 24-25 0.4052795827418407 1103 26-27 0.0872490185306205 1103 28-29 0.31031670882263995 1103 30-31 0.24684346395222434 1103 32-33 0.2337800740051179 1103 34-35 0.30154951921741713 1103 36-37 0.29554089001855743 1103 38-39 0.4679581402126729 1103 40-41 0.43127265829152606 1103 42-43 0.4067397177901597 1103 44-45 0.3133212376560479 1103 46-47 0.561378385362822 1103 48-49 0.4244232609198235 1103 50-51 0.2178171841461065 1103 52-53 0.29827402722188623 1103 54-55 0.3109272526903979 1103 56-57 0.38571810630447345 1103 58-59 0.47510469537077427 1103 60-61 0.4691599428931639 1103 62-63 0.5686131629658959 1103 64-65 0.6167100213153702 1103 66-67 0.639983520788256 1103 68-69 0.7751417834048411 1103 70-71 0.945432979660179 1103 72-73 0.8880043743202712 1103 74-75 0.9772054959492493 1103 76-77 0.7837969236520443 1103 78-79 0.7881948836337891 1103 80-81 1.136356579240914 1103 82-83 0.9787507051010742 1103 84-85 1.0807011580331718 1103 86-87 0.9908083270481924 1103 88-89 1.1185639498806204 1103 90-91 1.2352508915671656 1103 92-93 1.2671513506710355 1103 94-95 1.2242149825275703 1103 96-97 1.3257783586783667 1103 98-99 1.7066375171799706 1103 100-101 1.6721840971841786 1104 1 0.24568344468245584 1104 2 0.375940853720774 1104 3 0.3183062809128536 1104 4 0.2585344252346289 1104 5 0.2646922490250887 1104 6 0.3431532942708486 1104 7 0.3459295591027711 1104 8 0.31194680943829667 1104 9 0.47306326514419794 1104 10-11 0.4616332637122724 1104 12-13 0.38944380375282606 1104 14-15 0.5470674239861779 1104 16-17 0.5689395843045872 1104 18-19 0.5708339888638889 1104 20-21 0.6768278639750633 1104 22-23 0.4830679841369161 1104 24-25 0.6218139201870301 1104 26-27 0.5743795757899903 1104 28-29 0.5668394603617486 1104 30-31 0.5498262668327953 1104 32-33 0.6336017419212467 1104 34-35 0.5509105905729683 1104 36-37 0.37809047088137504 1104 38-39 0.6778362610241331 1104 40-41 0.8085387252171685 1104 42-43 0.7827392861583604 1104 44-45 0.6595382121443336 1104 46-47 0.6323988537969072 1104 48-49 0.7571205052915673 1104 50-51 0.5952564717264792 1104 52-53 0.6778799928276484 1104 54-55 0.7848220481196435 1104 56-57 0.9494434294603877 1104 58-59 0.9028290871107032 1104 60-61 0.9012546065611318 1104 62-63 0.806337213943884 1104 64-65 0.8099305624453308 1104 66-67 0.8333034508184696 1104 68-69 0.8078066553919427 1104 70-71 0.8703716425103103 1104 72-73 0.9774827813717728 1104 74-75 0.8520862292689557 1104 76-77 0.8148545351379965 1104 78-79 0.8277743833952584 1104 80-81 1.0679656571390943 1104 82-83 1.0458296937196216 1104 84-85 1.1580115240115028 1104 86-87 1.1216410356512974 1104 88-89 1.2159565530746974 1104 90-91 1.2181957562846755 1104 92-93 1.3214602854491986 1104 94-95 1.2862734118421812 1104 96-97 1.4184230121234727 1104 98-99 1.57645225626991 1104 100-101 1.4397503753822285 1105 1 0.2274392463997792 1105 2 0.20825197230390913 1105 3 0.27989643195157043 1105 4 0.2818829516887149 1105 5 0.3730797026622028 1105 6 0.23268923990769963 1105 7 0.3214732009270236 1105 8 0.253767742320818 1105 9 0.3698180187022331 1105 10-11 0.376364986823873 1105 12-13 0.41217251522260057 1105 14-15 0.5038785913450496 1105 16-17 0.5848498120561061 1105 18-19 0.49635267204373434 1105 20-21 0.544324703930485 1105 22-23 0.4411592549418444 1105 24-25 0.5407100009015267 1105 26-27 0.551422033505581 1105 28-29 0.49086607322982445 1105 30-31 0.4611864973863291 1105 32-33 0.5055519616195809 1105 34-35 0.6075039276008027 1105 36-37 0.6193219957767155 1105 38-39 0.6144485686972914 1105 40-41 0.7780305443858211 1105 42-43 0.725185482377924 1105 44-45 0.7325912822132707 1105 46-47 0.8292317522997976 1105 48-49 0.8812145220662728 1105 50-51 0.6439605940020385 1105 52-53 0.6257402949880984 1105 54-55 0.5880220991784881 1105 56-57 0.7970356207774714 1105 58-59 1.0203967556516034 1105 60-61 0.9248618000471822 1105 62-63 0.9300983623113481 1105 64-65 0.8789022974291498 1105 66-67 0.9432859745784725 1105 68-69 1.0965397732818332 1105 70-71 1.110597816604379 1105 72-73 0.9404326855845433 1105 74-75 0.9168108435774691 1105 76-77 1.1219200627357218 1105 78-79 0.97198864790915 1105 80-81 0.9401748033147648 1105 82-83 1.1401456089287265 1105 84-85 1.179220663960482 1105 86-87 1.2940245810033844 1105 88-89 1.1889739753500557 1105 90-91 1.275216659279085 1105 92-93 1.3779436176409732 1105 94-95 1.3435350801381674 1105 96-97 1.174268575711455 1105 98-99 1.5139949350329545 1105 100-101 1.467579263897516 1106 1 -7.442243280060268E-4 1106 2 0.04600413916163504 1106 3 -0.012071892435233877 1106 4 0.014655309430111174 1106 5 -0.020509006808993036 1106 6 0.08623153993685406 1106 7 0.03727217468800603 1106 8 0.0890551381973026 1106 9 0.1164872717893104 1106 10-11 -0.24971150108022755 1106 12-13 -0.34848578357033233 1106 14-15 -0.20110901721702135 1106 16-17 -0.17865199661161313 1106 18-19 -0.26791556459473753 1106 20-21 -0.12971298693675237 1106 22-23 -0.21909981086356112 1106 24-25 -0.14618024500568083 1106 26-27 -0.08044357930435808 1106 28-29 0.1728712541075481 1106 30-31 0.16913159367204145 1106 32-33 0.35577858392256445 1106 34-35 0.08978219867373127 1106 36-37 0.1576932596230094 1106 38-39 0.1676681872075818 1106 40-41 -0.09245070881523532 1106 42-43 0.1976713392612055 1106 44-45 0.1450125479957265 1106 46-47 0.4301508265658711 1106 48-49 0.6178627659671747 1106 50-51 0.5064270490790577 1106 52-53 0.5804983952696077 1106 54-55 0.5808561072460705 1106 56-57 0.6622527047418885 1106 58-59 0.3561023442923954 1106 60-61 0.25062894037949235 1106 62-63 0.5566991665858083 1106 64-65 0.5825117603669909 1106 66-67 0.8261638714241428 1106 68-69 0.7785287890319665 1106 70-71 0.489847235938381 1106 72-73 0.5373904816328725 1106 74-75 0.51993641209679 1106 76-77 0.6567727171409992 1106 78-79 0.487545071693404 1106 80-81 0.4375138589206138 1106 82-83 0.48128304575687153 1106 84-85 0.461698140549359 1106 86-87 0.3061573768873238 1106 88-89 0.8249703718965229 1106 90-91 0.974979917788886 1106 92-93 0.9255755817058038 1106 94-95 0.851314159650137 1106 96-97 0.6136948287554311 1106 98-99 0.789268792386757 1106 100-101 0.8077552866112576 1107 1 0.4111478930143626 1107 2 0.4738709869283859 1107 3 0.42161992879977106 1107 4 0.3633501223103437 1107 5 0.5094644396099852 1107 6 0.4240706112602979 1107 7 0.43992425726614925 1107 8 0.3572576616609737 1107 9 0.5352446690142898 1107 10-11 0.5927408182091156 1107 12-13 0.4667057690717016 1107 14-15 0.7467309930899546 1107 16-17 0.5724536019139634 1107 18-19 0.6196649587029768 1107 20-21 0.7036175440038477 1107 22-23 0.7190687781717315 1107 24-25 0.6686707623271246 1107 26-27 0.7041752152154714 1107 28-29 0.6483676051738456 1107 30-31 0.7013275922919462 1107 32-33 0.8153248801784301 1107 34-35 0.970636979410159 1107 36-37 0.9338646961804784 1107 38-39 0.9644629778142573 1107 40-41 0.9738540852416477 1107 42-43 1.0460881693401234 1107 44-45 1.0680350049880971 1107 46-47 0.8945448983042112 1107 48-49 0.9576530669223189 1107 50-51 0.8756793779766028 1107 52-53 0.8233531672712999 1107 54-55 1.0345345206696095 1107 56-57 1.0372120882446012 1107 58-59 1.0534208911826184 1107 60-61 1.100894912926229 1107 62-63 1.1236531694944816 1107 64-65 1.1490951923299946 1107 66-67 1.1891019727557506 1107 68-69 1.2613549984646042 1107 70-71 1.2822290222781412 1107 72-73 1.3036574979799234 1107 74-75 1.3306223723275608 1107 76-77 1.381942826019415 1107 78-79 1.3501653380153371 1107 80-81 1.4945966335951333 1107 82-83 1.5671638996641342 1107 84-85 1.6899879676453686 1107 86-87 1.5265376870576048 1107 88-89 1.805079206639789 1107 90-91 1.8784084087331543 1107 92-93 1.908225131212074 1107 94-95 2.148577022632786 1107 96-97 2.12848316439619 1107 98-99 2.250648399411226 1107 100-101 2.081721422060724 1108 1 0.2952563605026306 1108 2 0.3549290043168547 1108 3 0.35961817275431684 1108 4 0.4052983370728924 1108 5 0.3042682648925137 1108 6 0.2014495323785539 1108 7 0.2561124618907016 1108 8 0.3738740155131808 1108 9 0.3095846810531597 1108 10-11 0.45832968180330624 1108 12-13 0.5281912341748267 1108 14-15 0.4657178951632659 1108 16-17 0.36229886172269943 1108 18-19 0.3663392213222849 1108 20-21 0.41288668059974043 1108 22-23 0.39239385929831627 1108 24-25 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2316 40-41 -0.6113726088166729 2316 42-43 -0.4498400381458296 2316 44-45 -0.45981107409657085 2316 46-47 -0.6206426287940303 2316 48-49 -0.5117459268418116 2316 50-51 -0.5019563169168535 2316 52-53 -0.7492589139608512 2316 54-55 -0.6927615914421708 2316 56-57 -0.6810473972307349 2316 58-59 -0.5458028988172927 2316 60-61 -0.6175576429652594 2316 62-63 -0.42660639163769787 2316 64-65 -0.35055082099142965 2316 66-67 -0.33906448391471145 2316 68-69 -0.4735970030950867 2316 70-71 -0.4895329380485798 2316 72-73 -0.5035066220583744 2316 74-75 -0.3758157331089649 2316 76-77 -0.39441514137248745 2316 78-79 -0.09415283388280216 2316 80-81 -0.25403528718634405 2316 82-83 -0.2374638250730321 2316 84-85 -0.19993716118948868 2316 86-87 -0.039290740706121596 2316 88-89 0.09183315025217453 2316 90-91 0.0042523461474921476 2316 92-93 -0.10815457296971331 2316 94-95 -0.29115401732857293 2316 96-97 -0.18604032850516106 2316 98-99 -0.10231938532762896 2316 100-101 -0.10497228707367512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10315.0 3 2304.0 4 2321.0 5 2699.0 6 3054.0 7 3408.0 8 4333.0 9 5349.0 10 6672.0 11 8210.0 12 10514.0 13 13053.0 14 15122.0 15 18116.0 16 21875.0 17 27455.0 18 24140.0 19 18399.0 20 18670.0 21 19510.0 22 21377.0 23 23644.0 24 26585.0 25 29940.0 26 34300.0 27 40241.0 28 46988.0 29 56063.0 30 67698.0 31 83059.0 32 103409.0 33 132426.0 34 175029.0 35 238179.0 36 333705.0 37 429559.0 38 303715.0 39 33998.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.84623241006683 18.855179434548052 14.580328503185639 22.718259652199475 2 15.071942401256361 19.68261425517442 41.389302414570345 23.856140928998872 3 19.22087623411063 24.457074322613845 29.76905168013346 26.55299776314206 4 12.436163214655535 16.263817901484355 37.3172882776882 33.982730606171906 5 13.438176262626683 37.53078590277507 34.2911999840623 14.739837850535947 6 31.861714174782414 35.87856207165749 17.679714080205876 14.580009673354224 7 28.5470276906741 32.88379539760755 20.51490717236574 18.054269739352613 8 26.242915638023945 35.33690537814233 19.667607377027423 18.752571606806296 9 26.365010532149398 16.80016791705694 20.586722439907845 36.248099110885825 10-11 24.839712611016854 26.93018953237067 27.173897713083917 21.056200143528564 12-13 25.474491659412585 24.23234800182436 28.156002906195948 22.137157432567108 14-15 22.585499171302402 25.464194062289934 26.539126120701763 25.411180645705905 16-17 21.685670356525012 28.96278882449483 27.20042488188624 22.151115937093923 18-19 21.536157914984646 27.185480216556563 30.027074754735487 21.2512871137233 20-21 22.304473635424447 26.26441675020146 30.20287652762911 21.22823308674499 22-23 22.064018857206968 26.215101319535794 30.125118132330194 21.59576169092704 24-25 21.728732306388014 26.307762563516988 30.294645016173995 21.668860113921003 26-27 21.427672271216668 26.667247341597584 30.125975352423023 21.77910503476273 28-29 21.428036634213488 26.704134072975165 30.283102355995805 21.584726936815542 30-31 21.50151190234231 26.48643435772149 30.236595088080236 21.77545865185597 32-33 21.341085414408163 26.561544985084783 30.156212052172865 21.94115754833419 34-35 21.851580625953883 26.637934234794198 29.952512884900834 21.557972254351085 36-37 21.751242955720187 26.330898401654856 29.963118302425773 21.954740340199184 38-39 21.44763934881734 26.742925511289528 30.002073565973614 21.80736157391952 40-41 21.79143107091604 26.552806589997353 29.974995350117972 21.68076698896863 42-43 21.530620836162278 26.504788089355547 29.895188352805185 22.069402721676987 44-45 21.579968536751572 26.774888462245137 29.773519358856472 21.87162364214682 46-47 21.76751034289684 27.03690262642182 29.1617639434692 22.033823087212134 48-49 21.826761064648625 27.08425799718432 29.029598645421036 22.059382292746015 50-51 22.012933657248542 27.319997063360486 28.771082728204473 21.895986551186496 52-53 22.055212910599224 27.281035353561546 28.673792898174817 21.98995883766442 54-55 21.92237104871873 27.25067759950396 28.770366868028297 22.056584483749017 56-57 21.86594331741365 27.665952482307297 28.617732908043564 21.85037129223549 58-59 21.98353057344401 27.186684120479416 28.758128599045374 22.071656707031202 60-61 21.857532618564377 27.302125584453307 28.991019670556078 21.849322126426234 62-63 21.79585137332726 27.269151491162173 29.133381534441572 21.801615601068995 64-65 21.804781607574768 27.174961718732995 28.984864774080915 22.03539189961133 66-67 21.636362656192205 27.434612009693744 28.74156317438622 22.187462159727833 68-69 21.668544574849744 27.34199756072375 28.923255691471784 22.06620217295472 70-71 21.717984058304282 27.350307369379678 28.81784660415257 22.11386196816347 72-73 21.837707915053073 27.345706031508932 28.60457454067877 22.21201151275923 74-75 21.686749736272976 27.66422360153133 28.49821690046972 22.150809761725977 76-77 21.827011821614324 27.386121241063183 28.588230390740943 22.19863654658155 78-79 21.86612408657863 27.435125024080374 28.49777216322774 22.20097872611325 80-81 21.75900044954556 27.659327406852004 28.429277857309927 22.15239428629251 82-83 21.93567688173358 27.578628178723797 28.36313735669335 22.12255758284927 84-85 21.801754547819876 27.498592816574806 28.37563867468718 22.324013960918137 86-87 21.884295313757235 27.436765173279625 28.448825096183526 22.230114416779607 88-89 21.971577277994523 27.366391788871624 28.382706355832845 22.279324577301008 90-91 21.837910980547626 27.388916442871196 28.527121688891395 22.246050887689783 92-93 21.883259288501982 27.080088882825326 28.54862109082374 22.488030737848952 94-95 21.728875269022314 27.218879457269512 28.46940298816924 22.582842285538938 96-97 21.87992513126599 27.150988390452845 28.401620538599552 22.56746593968161 98-99 21.7300498494563 27.119908263729336 28.46700480246842 22.683037084345944 100-101 21.976567660734496 26.60966710469953 28.88611795206298 22.527647282503 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2858.0 1 1687.5 2 542.5 3 583.5 4 510.5 5 462.0 6 759.0 7 1602.5 8 2720.5 9 3396.0 10 3227.5 11 2979.5 12 3109.0 13 3346.0 14 3589.5 15 3694.0 16 3830.0 17 4061.0 18 4286.0 19 4631.5 20 5131.0 21 5876.5 22 6953.0 23 8383.5 24 10063.5 25 12511.5 26 15879.0 27 19407.0 28 22271.5 29 25472.0 30 29497.5 31 34060.0 32 39499.5 33 44495.0 34 50013.5 35 55111.0 36 60583.5 37 66644.5 38 70496.0 39 72693.5 40 74182.0 41 77938.0 42 81333.5 43 84397.5 44 89066.5 45 92318.0 46 92595.0 47 92729.0 48 93598.5 49 92087.0 50 89085.5 51 84733.0 52 80288.5 53 78063.5 54 75933.5 55 69607.0 56 61377.5 57 54512.0 58 49183.5 59 43267.5 60 35594.0 61 28853.0 62 22806.5 63 16712.5 64 12721.0 65 10166.5 66 7351.0 67 5362.0 68 3970.0 69 2893.5 70 2160.5 71 1486.0 72 1059.0 73 781.0 74 516.0 75 385.0 76 285.0 77 171.0 78 98.5 79 65.0 80 49.5 81 35.0 82 24.0 83 15.5 84 14.0 85 13.5 86 10.0 87 6.5 88 7.0 89 8.5 90 5.0 91 5.0 92 6.0 93 4.5 94 3.0 95 2.0 96 2.5 97 1.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28214356242783395 2 0.245918233429396 3 0.24041198342163342 4 0.24724304546133888 5 0.2506792766691907 6 0.1942091638076257 7 0.18700549838393632 8 0.18601188935997917 9 0.19553397583956855 10-11 0.19735559238349 12-13 0.1956374767795641 14-15 0.19567887715556234 16-17 0.1827412596561202 18-19 0.18710899932393185 20-21 0.1766140040083844 22-23 0.18528738278001042 24-25 0.1849768799600238 26-27 0.1842109730040568 28-29 0.19170444105973372 30-31 0.18907551718384705 32-33 0.19224264594771048 34-35 0.18930321925183727 36-37 0.18007093540423533 38-39 0.17106635362462363 40-41 0.16914123614070664 42-43 0.18009163559223443 44-45 0.18091964311219874 46-47 0.18058844010421302 48-49 0.1925531487676971 50-51 0.18704689875993452 52-53 0.20118512716332493 54-55 0.1801744363442309 56-57 0.1681476271167495 58-59 0.17059024930064415 60-61 0.1609853620690583 62-63 0.16611900869283697 64-65 0.1659948075648423 66-67 0.16498049835288606 68-69 0.16833392880874146 70-71 0.16922403689270307 72-73 0.16665721358081373 74-75 0.17911872675627638 76-77 0.17112845418862094 78-79 0.17559969479642815 80-81 0.17027974648065755 82-83 0.15475460548132697 84-85 0.1533676928853868 86-87 0.15614151807726717 88-89 0.16065415906107258 90-91 0.1659741073768432 92-93 0.163427984252953 94-95 0.16257927654498958 96-97 0.1662846101968298 98-99 0.17292937054454327 100-101 0.16137866564104136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2415437.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.30933296498114 #Duplication Level Percentage of deduplicated Percentage of total 1 76.37914753727526 40.71721407646026 2 12.958384948029172 13.816057157657738 3 4.412533739612397 7.056876910326319 4 1.9635231567908105 4.186964389992488 5 1.1030571449795417 2.940162031055793 6 0.6577459799710106 2.103839967159145 7 0.42842435347064123 1.5987311556631232 8 0.3199016872490795 1.3642996449296352 9 0.2387705762721299 1.1455830137458256 >10 1.3625798143163388 13.600191857697244 >50 0.11928300865235461 4.366149596975712 >100 0.054469134406144905 5.184689354768956 >500 0.0017120077661142744 0.6125607447719148 >1k 2.334556044700134E-4 0.27632853317920747 >5k 1.556370696466756E-4 0.5883337723386158 >10k+ 7.78185348233378E-5 0.44201779327795454 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10655 0.44112100626097883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8602 0.3561260343366438 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5580 0.23101409807003867 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3090 0.12792716183448377 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 6.210056399732223E-5 14-15 0.0 0.0 0.0 0.0 8.280075199642964E-5 16-17 0.0 0.0 0.0 4.140037599821482E-5 1.0350093999553705E-4 18-19 2.070018799910741E-5 0.0 0.0 6.210056399732223E-5 1.6560150399285928E-4 20-21 4.140037599821482E-5 0.0 0.0 1.0350093999553705E-4 1.6560150399285928E-4 22-23 4.140037599821482E-5 0.0 0.0 2.691024439883963E-4 1.6560150399285928E-4 24-25 4.140037599821482E-5 0.0 0.0 4.5540413598036295E-4 1.6560150399285928E-4 26-27 4.140037599821482E-5 0.0 0.0 7.245065799687592E-4 1.6560150399285928E-4 28-29 6.210056399732223E-5 0.0 0.0 0.001718115603925915 2.0700187999107407E-4 30-31 8.280075199642964E-5 0.0 0.0 0.003870935155833085 2.0700187999107407E-4 32-33 8.280075199642964E-5 0.0 0.0 0.006106555459736686 2.0700187999107407E-4 34-35 8.280075199642964E-5 0.0 0.0 0.008590578019629574 2.0700187999107407E-4 36-37 1.6560150399285928E-4 0.0 0.0 0.012358012235467123 2.0700187999107407E-4 38-39 1.6560150399285928E-4 0.0 0.0 0.018423167319205593 2.0700187999107407E-4 40-41 1.6560150399285928E-4 0.0 0.0 0.024653923906936923 2.0700187999107407E-4 42-43 2.0700187999107407E-4 0.0 0.0 0.029684069590720022 2.277020679901815E-4 44-45 2.0700187999107407E-4 0.0 0.0 0.032664896662591494 3.105028199866111E-4 46-47 2.277020679901815E-4 0.0 0.0 0.03692913539040761 3.7260338398393335E-4 48-49 2.484022559892889E-4 0.0 0.0 0.043118491602140735 3.7260338398393335E-4 50-51 2.484022559892889E-4 0.0 0.0 0.049287147625874744 4.1400375998214815E-4 52-53 2.484022559892889E-4 0.0 0.0 0.06352887696926064 4.1400375998214815E-4 54-55 2.484022559892889E-4 0.0 0.0 0.07452067679678667 4.5540413598036295E-4 56-57 2.484022559892889E-4 0.0 0.0 0.10020961010367896 4.5540413598036295E-4 58-59 2.484022559892889E-4 0.0 0.0 0.14156858572589556 4.5540413598036295E-4 60-61 2.484022559892889E-4 0.0 0.0 0.17713150870836208 4.5540413598036295E-4 62-63 2.484022559892889E-4 0.0 0.0 0.2006883226513463 4.5540413598036295E-4 64-65 2.484022559892889E-4 0.0 0.0 0.23952187533767183 4.761043239794704E-4 66-67 2.484022559892889E-4 0.0 0.0 0.3038373594508985 4.968045119785778E-4 68-69 2.484022559892889E-4 0.0 0.0 0.39400737837501043 4.968045119785778E-4 70-71 2.484022559892889E-4 0.0 0.0 0.4785262459753659 4.968045119785778E-4 72-73 2.484022559892889E-4 0.0 0.0 0.5729398034392947 5.175046999776852E-4 74-75 2.484022559892889E-4 0.0 0.0 0.6875981447663507 5.382048879767926E-4 76-77 2.484022559892889E-4 0.0 0.0 0.7818461007262868 5.382048879767926E-4 78-79 2.484022559892889E-4 2.070018799910741E-5 0.0 0.8987607625452454 5.382048879767926E-4 80-81 2.484022559892889E-4 4.140037599821482E-5 0.0 1.0523354573106234 5.382048879767926E-4 82-83 2.484022559892889E-4 4.140037599821482E-5 0.0 1.1971125721763805 6.003054519741148E-4 84-85 2.484022559892889E-4 4.140037599821482E-5 0.0 1.367019715273054 6.210056399732222E-4 86-87 2.484022559892889E-4 4.140037599821482E-5 0.0 1.5860484044916094 6.417058279723296E-4 88-89 2.484022559892889E-4 4.140037599821482E-5 0.0 1.8726632075272507 6.624060159714371E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5300 0.0 55.9694 1 GTATCAA 9840 0.0 43.479977 1 ATCAACG 12675 0.0 33.556892 3 TCAACGC 12910 0.0 32.877213 4 TATCAAC 12960 0.0 32.745613 2 CAACGCA 13045 0.0 32.572735 5 AACGCAG 13535 0.0 31.590445 6 CGCAGAG 15480 0.0 27.4064 8 ACGCAGA 15665 0.0 27.143393 7 GTACATG 10970 0.0 26.867483 1 TACATGG 11050 0.0 25.288422 2 GCAGAGT 16940 0.0 24.314657 9 ACATGGG 11115 0.0 24.071636 3 GAGTACT 9980 0.0 22.941664 12-13 TGGTATC 2455 0.0 22.261429 2 CATGGGG 5085 0.0 21.778841 4 GGACCGA 635 0.0 21.696802 6 GTACTTT 10585 0.0 21.383581 14-15 CAGAGTA 16090 0.0 21.344728 10-11 AGTACTT 10430 0.0 20.67664 12-13 >>END_MODULE