FastQCFastQC Report
Thu 26 May 2016
SRR937774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415437
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128460.5318292300730675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111390.4611587882441148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72080.2984139101951324No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29500.1221311091947337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62150.056.80771
GTATCAA110000.041.7727781
ATCAACG141350.032.265993
TCAACGC142950.031.9048484
TATCAAC144650.031.5298882
CAACGCA144800.031.496575
AACGCAG148600.030.8126416
ACGCAGA173650.026.2599647
GTACATG112800.025.9496271
CGCAGAG174700.025.9389958
TGGTATC26750.025.752172
TACATGG114800.024.6645972
ACATGGG112650.024.4183863
GCAGAGT186850.023.7947129
GAGTACT117600.022.78082712-13
GTGGTAT32350.022.3269231
GTGTAAG10750.021.6594371
CAGAGTA180750.021.49674210-11
GTACTTT123200.021.45483414-15
CATGGGG48500.021.2562984
Produced by FastQC (version 0.11.5)