##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937774_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2415437 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2226595021936 33.0 31.0 34.0 31.0 34.0 2 32.43875000672756 34.0 31.0 34.0 31.0 34.0 3 32.5251033249884 34.0 31.0 34.0 31.0 34.0 4 36.14643561392825 37.0 35.0 37.0 35.0 37.0 5 36.065307437122144 37.0 35.0 37.0 35.0 37.0 6 36.10030648698351 37.0 35.0 37.0 35.0 37.0 7 36.04741626463451 37.0 35.0 37.0 35.0 37.0 8 36.02539250661474 37.0 35.0 37.0 35.0 37.0 9 37.70718921669246 39.0 37.0 39.0 35.0 39.0 10-11 37.64123034465399 39.0 37.0 39.0 35.0 39.0 12-13 37.54550750029912 39.0 37.0 39.0 35.0 39.0 14-15 38.77767625485575 40.0 38.0 41.0 35.0 41.0 16-17 38.73966284361795 40.0 38.0 41.0 35.0 41.0 18-19 38.73148751136958 40.0 38.0 41.0 35.0 41.0 20-21 38.62271858053015 40.0 38.0 41.0 34.0 41.0 22-23 38.47012590268345 40.0 38.0 41.0 34.0 41.0 24-25 38.546244012988126 40.0 38.0 41.0 34.0 41.0 26-27 38.430248439516326 40.0 38.0 41.0 34.0 41.0 28-29 38.29205191441549 40.0 38.0 41.0 34.0 41.0 30-31 38.316845978595175 40.0 38.0 41.0 34.0 41.0 32-33 38.243388463454025 40.0 38.0 41.0 34.0 41.0 34-35 38.116119153594155 40.0 37.5 41.0 34.0 41.0 36-37 37.95874618133282 40.0 37.0 41.0 33.0 41.0 38-39 37.819895530291205 40.0 37.0 41.0 33.0 41.0 40-41 37.64556765504544 40.0 37.0 41.0 33.0 41.0 42-43 37.428134536317856 40.0 37.0 41.0 33.0 41.0 44-45 37.114164641843274 40.0 36.5 41.0 31.5 41.0 46-47 36.75265862864566 40.0 36.0 41.0 31.0 41.0 48-49 36.41209209762043 39.0 36.0 41.0 30.0 41.0 50-51 36.153553787575504 39.0 36.0 41.0 30.0 41.0 52-53 35.90239964859361 39.0 35.0 40.5 29.0 41.0 54-55 35.67778998168861 39.0 35.0 40.0 28.0 41.0 56-57 35.413882042876715 38.5 35.0 40.0 28.0 41.0 58-59 35.143738793435716 38.0 34.5 40.0 27.5 41.0 60-61 34.79465289303758 38.0 34.0 40.0 26.5 41.0 62-63 34.43812113501615 37.5 34.0 40.0 26.0 41.0 64-65 34.03953570306325 37.0 33.5 39.5 26.0 41.0 66-67 33.60318857415864 36.0 33.0 39.0 25.0 40.5 68-69 33.16599253054416 36.0 32.5 39.0 23.5 40.0 70-71 32.74595632177531 35.0 32.0 38.5 22.5 40.0 72-73 32.2493952440076 35.0 31.5 37.5 21.5 39.5 74-75 31.73774642021299 35.0 31.0 37.0 20.0 39.0 76-77 30.502390457710135 33.5 29.5 35.5 20.0 38.0 78-79 31.09954741108959 34.0 31.0 36.0 20.0 38.5 80-81 30.90282420944947 34.0 31.0 36.0 18.5 37.5 82-83 30.543229651611696 34.0 31.0 35.0 17.5 37.0 84-85 30.15374774833705 34.0 30.0 35.0 13.5 36.5 86-87 29.72862736639374 34.0 30.0 35.0 7.5 36.0 88-89 29.353251399229208 34.0 29.5 35.0 4.5 36.0 90-91 28.820586502566616 34.0 29.0 35.0 2.0 35.0 92-93 28.333151723683955 33.5 29.0 35.0 2.0 35.0 94-95 27.850787041847916 33.0 27.5 35.0 2.0 35.0 96-97 27.288379701064443 33.0 26.5 35.0 2.0 35.0 98-99 26.39813064882255 33.0 25.0 34.5 2.0 35.0 100-101 23.724613599940717 30.0 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24983756956875425 1101 2 0.26763495217819866 1101 3 0.13777071330640211 1101 4 0.1360324889291249 1101 5 0.05366025682017295 1101 6 0.12982495816830664 1101 7 0.10842614317513011 1101 8 0.15497849159030608 1101 9 0.21434138952210446 1101 10-11 0.2502649987013683 1101 12-13 0.19948935158910075 1101 14-15 0.23814700396532373 1101 16-17 0.2914100904786565 1101 18-19 0.28434377662817667 1101 20-21 0.27742708799685545 1101 22-23 0.18532352268299945 1101 24-25 0.32156897613520385 1101 26-27 0.1218507618634419 1101 28-29 0.1621484846026391 1101 30-31 0.16570942774578157 1101 32-33 0.2211664726847431 1101 34-35 0.1522193050138938 1101 36-37 0.11309523860704473 1101 38-39 0.07328220823774245 1101 40-41 0.09598772763762753 1101 42-43 0.23109893838086748 1101 44-45 0.25365609479091944 1101 46-47 0.21785688836517636 1101 48-49 0.046378329695713205 1101 50-51 0.008219596672354612 1101 52-53 -0.0032716079658641206 1101 54-55 0.016725029657379764 1101 56-57 -0.0012885999270295656 1101 58-59 -0.03744640951826028 1101 60-61 0.043884637587431996 1101 62-63 0.027483466011709368 1101 64-65 0.03181794438900454 1101 66-67 0.11735271338020681 1101 68-69 0.09089067160920905 1101 70-71 0.09550774534218931 1101 72-73 0.04573954335868535 1101 74-75 -0.007178970488894265 1101 76-77 -0.08996816072576763 1101 78-79 0.05272547947091866 1101 80-81 -0.01791491904832654 1101 82-83 -0.01107781071669578 1101 84-85 0.00902350608217617 1101 86-87 -0.15068285454778874 1101 88-89 0.020272571820473928 1101 90-91 -0.010051025185784823 1101 92-93 0.16463852620347907 1101 94-95 0.14990878725985723 1101 96-97 0.07636680622300318 1101 98-99 0.1673424198911313 1101 100-101 0.3866693419196956 1102 1 0.23516718056457364 1102 2 0.19325682376593534 1102 3 0.10724319794573489 1102 4 0.06758490429715636 1102 5 -0.009127866167830234 1102 6 0.09554812804304191 1102 7 0.0877188728570033 1102 8 0.12867907181084348 1102 9 0.13117142573710794 1102 10-11 0.1872598135679837 1102 12-13 0.16528978642056558 1102 14-15 0.17652473059981588 1102 16-17 0.20568337689251592 1102 18-19 0.17269931905305924 1102 20-21 0.1664732350815683 1102 22-23 0.22608013256909487 1102 24-25 0.21032544511096063 1102 26-27 0.2255616143945005 1102 28-29 0.15849097108323917 1102 30-31 0.06399607078246561 1102 32-33 0.040983807850430765 1102 34-35 0.06704064879030369 1102 36-37 0.10516680638635734 1102 38-39 0.101992491103438 1102 40-41 0.16945818924467204 1102 42-43 0.22320507895437203 1102 44-45 0.21763261829216418 1102 46-47 0.09161040162895517 1102 48-49 0.04585698767414215 1102 50-51 -0.03650692597767602 1102 52-53 -0.06313806792913113 1102 54-55 -0.030228426194604197 1102 56-57 -0.20574025314828503 1102 58-59 -0.02494487404094059 1102 60-61 -0.13052266816782065 1102 62-63 -0.06018777081167315 1102 64-65 -0.11724789609264974 1102 66-67 -0.11701134757162635 1102 68-69 -0.1172015361282277 1102 70-71 -0.10980920991563181 1102 72-73 0.10537650020895484 1102 74-75 -0.10219581293988611 1102 76-77 -0.1681641601820303 1102 78-79 -0.05286356629638789 1102 80-81 -0.09800666005304848 1102 82-83 0.09531459416425037 1102 84-85 -0.11423433284055662 1102 86-87 -0.010356990247576192 1102 88-89 0.13043373866275587 1102 90-91 0.08383761793942668 1102 92-93 0.1267896323626445 1102 94-95 0.18999993633643442 1102 96-97 0.22538779374248818 1102 98-99 0.12932452532715288 1102 100-101 0.5467668502950822 1103 1 0.2095803707988324 1103 2 0.22839832505919588 1103 3 0.15869100163322258 1103 4 0.11067718939523274 1103 5 0.03300686208088166 1103 6 0.0880285231799931 1103 7 0.15768538063603899 1103 8 0.09723056665729501 1103 9 0.15115096074305256 1103 10-11 0.18127995455152046 1103 12-13 0.14193597695878424 1103 14-15 0.17751944844901146 1103 16-17 0.23537612918925532 1103 18-19 0.20604790648968674 1103 20-21 0.17953804052495315 1103 22-23 0.20723045897350545 1103 24-25 0.2950612930331218 1103 26-27 0.1449172343067815 1103 28-29 0.10587419646873286 1103 30-31 0.19865055420837763 1103 32-33 0.21647642074471918 1103 34-35 0.20720122327988122 1103 36-37 0.1937643363350574 1103 38-39 0.24056154487025339 1103 40-41 0.11105209272516703 1103 42-43 0.2770835182257798 1103 44-45 0.24477810235385533 1103 46-47 0.265792080226106 1103 48-49 0.22633613353342952 1103 50-51 0.1711160337860136 1103 52-53 0.17707202672425382 1103 54-55 0.23271918987854434 1103 56-57 0.07474276190874463 1103 58-59 0.11357755450138285 1103 60-61 0.13707995464719858 1103 62-63 0.24200909842280538 1103 64-65 0.2871317017112034 1103 66-67 0.30683449022937026 1103 68-69 0.2532073406464477 1103 70-71 0.3934527464620814 1103 72-73 0.5084412666820057 1103 74-75 0.42900342123732216 1103 76-77 0.2555868961924581 1103 78-79 0.3167931829762587 1103 80-81 0.20366438799292652 1103 82-83 0.39693484159248626 1103 84-85 0.3295363423149631 1103 86-87 0.4342138294322204 1103 88-89 0.48634397995594725 1103 90-91 0.5445442447895452 1103 92-93 0.6315559829242083 1103 94-95 0.6245690190526467 1103 96-97 0.8216900376544878 1103 98-99 0.8801822902781247 1103 100-101 1.1896166563898234 1104 1 0.17796902115436097 1104 2 0.2067088273423039 1104 3 0.09606463574576196 1104 4 0.052469370044498476 1104 5 0.02046114318676473 1104 6 0.0986489370921646 1104 7 0.09646749744923255 1104 8 0.08002208850013659 1104 9 0.1518268052647329 1104 10-11 0.1501996256026601 1104 12-13 0.18152399576884903 1104 14-15 0.17862408508319305 1104 16-17 0.29660253142466075 1104 18-19 0.22908057420135464 1104 20-21 0.23866591712369 1104 22-23 0.17783122228025405 1104 24-25 0.09155542056895882 1104 26-27 -0.0027604730468695493 1104 28-29 0.02414244427082224 1104 30-31 0.25205646983572905 1104 32-33 0.21208059167090454 1104 34-35 0.16680957421925058 1104 36-37 0.16948220816312443 1104 38-39 0.2064910298653757 1104 40-41 0.19750907720914057 1104 42-43 0.17789623546151745 1104 44-45 0.16691115853414118 1104 46-47 0.07831337784759285 1104 48-49 0.003358555669322527 1104 50-51 -0.03771992341428643 1104 52-53 0.07136483210374678 1104 54-55 0.02469538197740917 1104 56-57 0.17545211468821975 1104 58-59 0.13856108434077186 1104 60-61 0.13866449768543276 1104 62-63 0.08756726648659452 1104 64-65 0.02419694626469493 1104 66-67 0.05936748734425379 1104 68-69 0.01561675476308011 1104 70-71 -0.038059782218716975 1104 72-73 0.03243090751890776 1104 74-75 0.10153119333573457 1104 76-77 -0.05350974389477514 1104 78-79 -0.024900176709333977 1104 80-81 0.10389213055863422 1104 82-83 0.09712112478838009 1104 84-85 0.12116041373866793 1104 86-87 0.149402157199777 1104 88-89 0.1438413106540537 1104 90-91 0.32981026628026555 1104 92-93 0.25507650815194793 1104 94-95 0.2017232024734419 1104 96-97 0.24025645215731473 1104 98-99 0.24481176845799624 1104 100-101 0.38425703895189756 1105 1 0.1820402378701118 1105 2 0.20469241794165782 1105 3 0.16570081031721173 1105 4 0.07824319179930228 1105 5 0.06306155373311384 1105 6 0.05397244471051721 1105 7 0.059735222237890184 1105 8 0.03264736429056114 1105 9 0.20426434638682167 1105 10-11 0.20721015078235183 1105 12-13 0.24458712194024912 1105 14-15 0.24682864011362682 1105 16-17 0.1934470530800425 1105 18-19 0.22709400101803823 1105 20-21 0.27738294940076713 1105 22-23 0.23061823631314127 1105 24-25 0.20642952729856034 1105 26-27 0.09393455618744184 1105 28-29 0.13601865977237537 1105 30-31 0.1823411644270152 1105 32-33 0.12830827677479562 1105 34-35 0.1152071877369707 1105 36-37 0.03616413021743625 1105 38-39 0.021869705946791385 1105 40-41 -0.028328516217136723 1105 42-43 0.05689532636495187 1105 44-45 0.10810112028151053 1105 46-47 0.036270255951848185 1105 48-49 0.058364087044566304 1105 50-51 0.10251263303582903 1105 52-53 0.04181115078153397 1105 54-55 -0.061740399067304 1105 56-57 -0.08800324099062351 1105 58-59 0.05280738516871253 1105 60-61 0.06666373802939773 1105 62-63 -0.019093059335276052 1105 64-65 0.13720590024323798 1105 66-67 0.09878761656880641 1105 68-69 0.04865608460531945 1105 70-71 0.010901450416604064 1105 72-73 0.09949520015668156 1105 74-75 0.11597062529567026 1105 76-77 0.1048941066895992 1105 78-79 0.3466878572551799 1105 80-81 0.22599205023434976 1105 82-83 0.32614263177161007 1105 84-85 0.30090442418289953 1105 86-87 0.3701182990603513 1105 88-89 0.3347513308492829 1105 90-91 0.3233838045131705 1105 92-93 0.42752621207289465 1105 94-95 0.4022563108157513 1105 96-97 0.4887584146020778 1105 98-99 0.5292629999932714 1105 100-101 0.5471404732613685 1106 1 0.04580657012869693 1106 2 0.11311085108232533 1106 3 0.1552150308804201 1106 4 0.017321118308430528 1106 5 0.07190561377021965 1106 6 0.03684325447198944 1106 7 0.05647772070539503 1106 8 -0.0012774848531762473 1106 9 0.06220849285713115 1106 10-11 -0.007440733605385219 1106 12-13 -0.08971721498996743 1106 14-15 0.04961791918181291 1106 16-17 0.0792280059202497 1106 18-19 -0.0014364263185200343 1106 20-21 0.04307353461126695 1106 22-23 0.14119667754915355 1106 24-25 0.18845308345127165 1106 26-27 0.13890858969546827 1106 28-29 0.19219302615417178 1106 30-31 0.1651980009425955 1106 32-33 0.12042307365677374 1106 34-35 0.003063578569943104 1106 36-37 0.0067233230789227605 1106 38-39 0.014801709392436635 1106 40-41 0.11993794280527226 1106 42-43 0.07356129893574348 1106 44-45 0.10874242833806846 1106 46-47 0.18537693629586727 1106 48-49 0.23327907856564423 1106 50-51 0.38410121026349486 1106 52-53 0.27585863570686087 1106 54-55 0.28027388177265067 1106 56-57 0.12039320027023592 1106 58-59 0.10153227625045247 1106 60-61 0.235848237480333 1106 62-63 0.22815119738667278 1106 64-65 0.23490784013161203 1106 66-67 0.21741554245689798 1106 68-69 0.07613908473609854 1106 70-71 0.015416270094149809 1106 72-73 0.1918120520008202 1106 74-75 0.18793658610336195 1106 76-77 0.11323969629481923 1106 78-79 0.13794729056608546 1106 80-81 0.1838894730929006 1106 82-83 0.12864367839512525 1106 84-85 0.28242247761658845 1106 86-87 0.3474828279847877 1106 88-89 0.07408791766569678 1106 90-91 0.21942669087068012 1106 92-93 0.34203880699050515 1106 94-95 0.5882312634528404 1106 96-97 0.6148734430928862 1106 98-99 0.6100245254552661 1106 100-101 0.7519232322120999 1107 1 0.24343181524707092 1107 2 0.33824110775238836 1107 3 0.21874425510532802 1107 4 0.09407865528909554 1107 5 0.1287925121110547 1107 6 0.22032980953791537 1107 7 0.25905315602253154 1107 8 0.19730268468794065 1107 9 0.2767133864899449 1107 10-11 0.3216300875903144 1107 12-13 0.34701232717912944 1107 14-15 0.4392203142449418 1107 16-17 0.4379552028141802 1107 18-19 0.4898433645720317 1107 20-21 0.3994813329787448 1107 22-23 0.40530927259393934 1107 24-25 0.3450638551210474 1107 26-27 0.3572608046047776 1107 28-29 0.3656083276552309 1107 30-31 0.4434350921026109 1107 32-33 0.413942231104663 1107 34-35 0.3515243006701141 1107 36-37 0.27146667485708775 1107 38-39 0.24987634766539912 1107 40-41 0.26491012268831327 1107 42-43 0.36845497542752526 1107 44-45 0.2890279230077013 1107 46-47 0.28857483035231724 1107 48-49 0.40640897274438004 1107 50-51 0.39359968386932564 1107 52-53 0.22180703091847676 1107 54-55 0.38892039159267 1107 56-57 0.4303145440211935 1107 58-59 0.24732224528583657 1107 60-61 0.38029611515916173 1107 62-63 0.48458891220645484 1107 64-65 0.5533129001610462 1107 66-67 0.3925329153993218 1107 68-69 0.40293101471849724 1107 70-71 0.5203126325155907 1107 72-73 0.5106525383506195 1107 74-75 0.43276333272192957 1107 76-77 0.36198386040293684 1107 78-79 0.592755104656078 1107 80-81 0.39498498300463325 1107 82-83 0.48776361061361584 1107 84-85 0.5422903211426267 1107 86-87 0.50888787491213 1107 88-89 0.5989304741466484 1107 90-91 0.7457031625636041 1107 92-93 0.5670236303960898 1107 94-95 0.7814701452326318 1107 96-97 0.913017749577115 1107 98-99 0.942206548506217 1107 100-101 0.913422723414044 1108 1 0.1720044399466687 1108 2 0.18997158192991748 1108 3 0.13266249942194008 1108 4 0.06995905411055503 1108 5 -0.016092326608841745 1108 6 0.037559195238820564 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2316 62-63 -0.18411811253042032 2316 64-65 -0.1993752452862978 2316 66-67 -0.2958812591991844 2316 68-69 -0.13773598755411598 2316 70-71 -0.006658300711428922 2316 72-73 -0.18335398559622007 2316 74-75 0.02923248655845967 2316 76-77 0.003216542990518434 2316 78-79 -0.07921098362380619 2316 80-81 0.0031378796428533917 2316 82-83 0.03390671260193656 2316 84-85 0.01555101421740801 2316 86-87 0.08438859219709727 2316 88-89 -0.011985206313809726 2316 90-91 0.18075730084683173 2316 92-93 0.10885458393406822 2316 94-95 0.0570575768868693 2316 96-97 0.20272725644006329 2316 98-99 0.2734995237693454 2316 100-101 0.912349269359563 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 5.0 4 16.0 5 55.0 6 93.0 7 235.0 8 551.0 9 1076.0 10 1838.0 11 3133.0 12 4869.0 13 7080.0 14 9118.0 15 12676.0 16 17818.0 17 25244.0 18 23329.0 19 14269.0 20 11895.0 21 12221.0 22 12628.0 23 13589.0 24 15479.0 25 18576.0 26 22411.0 27 27561.0 28 35159.0 29 43888.0 30 54988.0 31 69432.0 32 89098.0 33 118056.0 34 162242.0 35 234485.0 36 360111.0 37 538924.0 38 416189.0 39 37094.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.914317871903954 19.825408268944646 15.12565163396609 22.134622225185318 2 15.560786723065018 20.652991570469442 40.31891537638945 23.46730633007609 3 19.511044999310684 24.742976115709084 29.940544920028966 25.80543396495127 4 12.936656025363588 16.995020764410164 36.712785420076166 33.35553779015008 5 13.422552276271471 37.88583486752151 34.04055060170818 14.651062254498836 6 31.991974951116507 36.561293049663476 17.00193381156288 14.44479818765714 7 28.574870716975852 33.34353162595423 20.092844483213597 17.988753173856324 8 26.45248044142737 35.90468308633179 19.097786446096503 18.545050026144338 9 26.603302011188866 16.827348425978407 20.23050073340766 36.33884882942507 10-11 24.96111469684368 27.416922900493784 26.605868834500757 21.016093568161786 12-13 25.72190479818048 24.5889904356011 27.778303348543602 21.910801417674815 14-15 22.661799914466823 25.833876023262043 26.358749990167414 25.14557407210372 16-17 21.650864833154415 29.404617052732075 26.96714921564918 21.977368898464338 18-19 21.511200664724438 27.440169211616777 30.036780094036814 21.01185002962197 20-21 22.20196593825465 26.467632979042715 30.418325959236363 20.91207512346627 22-23 22.070007816458943 26.332627588788014 30.289295922524317 21.308068672228718 24-25 21.822085122520324 26.500847052043607 30.430323966356383 21.246743859079686 26-27 21.43840110953559 26.900648954117806 30.280654543196334 21.380295393150273 28-29 21.413102294476836 26.95232981246654 30.408680269055882 21.225887624000737 30-31 21.48727124739747 26.73209858091931 30.334800700659965 21.445829471023256 32-33 21.461333911834586 26.744974097854758 30.266283078382916 21.527408911927736 34-35 21.821103179259076 26.82030208198351 30.164148350795323 21.194446387962095 36-37 21.839215843758293 26.50348570465717 30.105856621389837 21.5514418301947 38-39 21.5628699515553 26.915074699810738 30.087195492156795 21.434859856477168 40-41 21.82172194648268 26.79919277095714 30.083254551427522 21.295830731132657 42-43 21.70726763847865 26.73848670692293 29.90691316194435 21.647332492654062 44-45 21.71433160955968 27.089632228039896 29.705204482667114 21.490831679733315 46-47 21.93810064183003 27.381670480331305 29.04401977778762 21.636209100051047 48-49 21.980680928544196 27.457660870476026 28.88595314222644 21.675705058753344 50-51 22.21289563751818 27.691324592609952 28.539080091925396 21.556699677946476 52-53 22.33119721193308 27.705462821013338 28.38641620543198 21.576923761621604 54-55 22.12920477743779 27.76352684835084 28.455140829589016 21.65212754462236 56-57 22.075756892024092 28.104189842252147 28.344042920597808 21.476010345125953 58-59 22.112334124218517 27.672735823786752 28.452615406653127 21.76231464534161 60-61 22.021915703038413 27.850281336255094 28.670898889103714 21.45690407160278 62-63 21.94615301496168 27.822191181140305 28.80058556691812 21.43107023697989 64-65 22.00067731015133 27.617838925213118 28.687127836495012 21.694355928140542 66-67 21.846191807113993 27.93279228561954 28.47337769521623 21.74763821205024 68-69 21.884468405166853 27.79905644305403 28.581172455721738 21.735302696057378 70-71 21.94862542414703 27.842737294633352 28.422653301515748 21.78598397970387 72-73 22.068118522652423 27.83533580051974 28.222470716479048 21.874074960348793 74-75 21.950463197852645 28.22874871684683 27.948033409282342 21.872754676018186 76-77 22.03831025193371 27.934986505547442 28.217461270983264 21.809241971535588 78-79 22.107759382670714 27.96574698491412 28.06798521344171 21.85850841897346 80-81 21.98682888437993 28.251388879113797 27.94920753470283 21.81257470180344 82-83 22.10226439997582 28.214765673064264 27.87910503745571 21.803864889504204 84-85 21.988771338885975 28.146836210258563 27.86554007450446 21.998852376351007 86-87 22.03220369647397 28.09456425483256 27.95554179223056 21.91769025646291 88-89 22.036819838397772 28.111641909931826 27.872389137038144 21.97914911463226 90-91 21.956694018597325 28.17722941421489 27.912839839250474 21.95323672793731 92-93 21.968903443764543 27.90755491889475 27.92800718727488 22.19553445006583 94-95 21.86593978646514 27.961544846750297 27.89975478557296 22.2727605812116 96-97 21.974802075152446 27.91828145383216 27.895283544965153 22.211632926050235 98-99 21.75556029826048 27.953236009440236 27.693735068500917 22.597468623798363 100-101 21.68200928818145 28.069637137322108 27.985786361888763 22.262567212607678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 598.0 1 450.0 2 378.5 3 477.5 4 415.5 5 392.5 6 750.5 7 1736.5 8 2913.0 9 3629.0 10 3606.0 11 3386.5 12 3614.5 13 3910.0 14 4232.0 15 4545.0 16 4689.5 17 4775.0 18 4975.0 19 5245.5 20 5641.5 21 6330.5 22 7371.0 23 8822.5 24 10516.0 25 12742.5 26 16065.0 27 19577.0 28 22381.5 29 25613.0 30 29504.0 31 34168.0 32 39611.5 33 44469.0 34 49682.0 35 54314.5 36 60242.5 37 66905.5 38 70832.5 39 73216.0 40 74445.0 41 77952.0 42 81100.0 43 84342.5 44 89458.5 45 92082.0 46 92144.0 47 92904.5 48 93418.5 49 91631.5 50 89848.0 51 86054.5 52 80631.5 53 77952.5 54 76295.5 55 69599.0 56 60597.0 57 54035.5 58 48988.0 59 43029.0 60 35079.0 61 28007.5 62 21927.0 63 16092.5 64 11974.5 65 9033.5 66 6522.0 67 5049.0 68 3880.0 69 2781.5 70 1970.5 71 1255.0 72 901.0 73 672.0 74 470.0 75 312.5 76 207.0 77 128.5 78 78.5 79 62.5 80 38.5 81 22.0 82 16.5 83 8.5 84 4.0 85 3.0 86 2.0 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02260460529502529 2 0.0 3 0.0 4 2.484022559892889E-4 5 0.019830780103144895 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003042927635868789 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.694078959625112E-4 24-25 4.140037599821481E-5 26-27 4.968045119785778E-4 28-29 0.0011592105279500148 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.0350093999553702E-4 40-41 5.589050759759E-4 42-43 0.01351722276341714 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.0350093999553702E-4 70-71 4.140037599821481E-5 72-73 0.0 74-75 2.0700187999107406E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0021114191759089556 84-85 4.55404135980363E-4 86-87 0.0 88-89 0.0 90-91 0.010391494375551919 92-93 0.0023598214318982444 94-95 0.0 96-97 0.0 98-99 0.0013041118439437666 100-101 0.017905662619227908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2415437.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.29265641172627 #Duplication Level Percentage of deduplicated Percentage of total 1 73.99656359296458 37.2148374842942 2 14.130696863814027 14.213405644601135 3 4.823276823495331 7.277262121880796 4 2.2671112578753347 4.560761901579229 5 1.1971509478403157 3.0103950646352713 6 0.7574273919353969 2.2855821347662104 7 0.48950930880562094 1.7233106434671894 8 0.35583330560963383 1.431664174309928 9 0.2676826161938935 1.2116222859268329 >10 1.5173273989410894 14.264220016200923 >50 0.13129227037323576 4.554662466388396 >100 0.06274301275332052 5.660388811775928 >500 0.0027246807352204307 0.9483405591253984 >1k 4.128304144270969E-4 0.34826746078383836 >5k 8.256608288541939E-5 0.2993098673339581 >10k+ 1.6513216577083878E-4 0.9959693629307693 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12846 0.5318292300730675 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11139 0.4611587882441148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7208 0.2984139101951324 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2950 0.1221311091947337 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.140037599821482E-5 0.0 0.0 0.0 0.0 3 4.140037599821482E-5 0.0 0.0 0.0 0.0 4 4.140037599821482E-5 0.0 0.0 0.0 0.0 5 8.280075199642964E-5 0.0 0.0 0.0 0.0 6 8.280075199642964E-5 0.0 0.0 0.0 0.0 7 8.280075199642964E-5 0.0 0.0 0.0 0.0 8 8.280075199642964E-5 0.0 0.0 0.0 0.0 9 8.280075199642964E-5 0.0 0.0 0.0 0.0 10-11 8.280075199642964E-5 0.0 0.0 0.0 0.0 12-13 8.280075199642964E-5 0.0 0.0 2.070018799910741E-5 0.0 14-15 8.280075199642964E-5 0.0 0.0 4.140037599821482E-5 0.0 16-17 8.280075199642964E-5 0.0 0.0 4.140037599821482E-5 0.0 18-19 8.280075199642964E-5 0.0 0.0 1.2420112799464445E-4 0.0 20-21 8.280075199642964E-5 0.0 0.0 1.8630169199196668E-4 4.140037599821482E-5 22-23 8.280075199642964E-5 0.0 0.0 3.519031959848259E-4 4.140037599821482E-5 24-25 8.280075199642964E-5 0.0 0.0 5.382048879767926E-4 4.140037599821482E-5 26-27 8.280075199642964E-5 0.0 0.0 8.487077079634038E-4 4.140037599821482E-5 28-29 8.280075199642964E-5 0.0 0.0 0.0018423167319205594 4.140037599821482E-5 30-31 8.280075199642964E-5 0.0 0.0 0.004036536659825944 6.210056399732223E-5 32-33 8.280075199642964E-5 0.0 0.0 0.006375657903725082 1.2420112799464445E-4 34-35 8.280075199642964E-5 0.0 0.0 0.008818280087619756 1.6560150399285928E-4 36-37 8.280075199642964E-5 0.0 0.0 0.012668515055453734 1.6560150399285928E-4 38-39 8.280075199642964E-5 0.0 0.0 0.018795770703189524 1.6560150399285928E-4 40-41 8.280075199642964E-5 0.0 0.0 0.025026527290920857 1.6560150399285928E-4 42-43 8.280075199642964E-5 0.0 0.0 0.030035972786704848 1.6560150399285928E-4 44-45 8.280075199642964E-5 0.0 0.0 0.03301679985857632 1.6560150399285928E-4 46-47 1.0350093999553705E-4 0.0 0.0 0.03734313915038977 1.6560150399285928E-4 48-49 1.2420112799464445E-4 0.0 0.0 0.04357389573812109 2.0700187999107407E-4 50-51 1.2420112799464445E-4 0.0 0.0 0.04970115138585689 2.0700187999107407E-4 52-53 1.2420112799464445E-4 0.0 0.0 0.0640049812932401 2.484022559892889E-4 54-55 1.2420112799464445E-4 0.0 0.0 0.07512098224876078 2.484022559892889E-4 56-57 1.2420112799464445E-4 0.0 0.0 0.10083061574365218 2.691024439883963E-4 58-59 1.2420112799464445E-4 0.0 0.0 0.14272779625384557 2.898026319875037E-4 60-61 1.2420112799464445E-4 0.0 0.0 0.1784770209283041 2.898026319875037E-4 62-63 1.2420112799464445E-4 0.0 0.0 0.2023029373152767 2.898026319875037E-4 64-65 1.2420112799464445E-4 0.0 0.0 0.24175749564157542 2.898026319875037E-4 66-67 1.2420112799464445E-4 0.0 0.0 0.3062592814467941 2.898026319875037E-4 68-69 1.2420112799464445E-4 0.0 0.0 0.3968847045068863 2.898026319875037E-4 70-71 1.2420112799464445E-4 0.0 0.0 0.4823764809431999 2.898026319875037E-4 72-73 1.2420112799464445E-4 0.0 0.0 0.5770384406631182 3.312030079857185E-4 74-75 1.2420112799464445E-4 0.0 0.0 0.6926696908261321 3.9330357198304075E-4 76-77 1.4490131599375185E-4 0.0 0.0 0.7877870546820307 4.1400375998214815E-4 78-79 1.6560150399285928E-4 0.0 0.0 0.9063370313529188 4.5540413598036295E-4 80-81 1.6560150399285928E-4 0.0 0.0 1.0616919422862199 4.761043239794704E-4 82-83 1.6560150399285928E-4 0.0 0.0 1.2078352695599182 5.175046999776852E-4 84-85 1.6560150399285928E-4 0.0 0.0 1.3796261297645105 5.382048879767926E-4 86-87 1.6560150399285928E-4 0.0 0.0 1.6017598471829322 5.589050759759E-4 88-89 1.6560150399285928E-4 0.0 0.0 1.8944191051143127 5.796052639750074E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6215 0.0 56.8077 1 GTATCAA 11000 0.0 41.772778 1 ATCAACG 14135 0.0 32.26599 3 TCAACGC 14295 0.0 31.904848 4 TATCAAC 14465 0.0 31.529888 2 CAACGCA 14480 0.0 31.49657 5 AACGCAG 14860 0.0 30.812641 6 ACGCAGA 17365 0.0 26.259964 7 GTACATG 11280 0.0 25.949627 1 CGCAGAG 17470 0.0 25.938995 8 TGGTATC 2675 0.0 25.75217 2 TACATGG 11480 0.0 24.664597 2 ACATGGG 11265 0.0 24.418386 3 GCAGAGT 18685 0.0 23.794712 9 GAGTACT 11760 0.0 22.780827 12-13 GTGGTAT 3235 0.0 22.326923 1 GTGTAAG 1075 0.0 21.659437 1 CAGAGTA 18075 0.0 21.496742 10-11 GTACTTT 12320 0.0 21.454834 14-15 CATGGGG 4850 0.0 21.256298 4 >>END_MODULE