FastQCFastQC Report
Thu 26 May 2016
SRR937773_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937773_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257616
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102680.45481605374873313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84220.37304838378183003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53520.2370642305866011No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29520.1307574007271387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50400.054.514451
GTATCAA91050.043.5412061
ATCAACG117000.033.914073
TCAACGC119150.033.302854
TATCAAC120150.033.106982
CAACGCA119850.032.911555
AACGCAG122950.031.993776
ACGCAGA141700.027.290927
CGCAGAG141400.027.2816248
TGGTATC21800.026.8143022
GTACATG104550.025.8248981
TACATGG105850.025.0530852
GCAGAGT152250.024.7439989
ACATGGG104350.024.2724743
GTGGTAT27250.023.7239361
GAGTACT89800.022.5602412-13
CAGAGTA148100.021.31376510-11
AGAGTAC143850.020.87024110-11
AGTACTT93950.020.57778412-13
GTACTTT98150.020.39806614-15
Produced by FastQC (version 0.11.5)