FastQCFastQC Report
Thu 26 May 2016
SRR937773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257616
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119730.5303381974613929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105430.4669970446701299No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69450.3076253889058192No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28470.12610647692078725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59450.052.4258961
GTATCAA103750.041.6722681
ATCAACG132600.032.311053
TCAACGC135600.031.59624
TATCAAC138300.031.1510832
CAACGCA137900.031.1381055
AACGCAG142150.030.2392166
TGGTATC26900.026.6632272
ACGCAGA162950.026.2061377
CGCAGAG164100.025.9645928
GTACATG102400.025.1010071
TACATGG103150.024.3137932
GCAGAGT176100.023.844639
ACATGGG100900.023.6790813
GTACGCT1252.257035E-422.7997073
GAGTACT109500.022.4054612-13
GTGGTAT33750.022.3829441
GTACCTG17400.022.1171721
AGTACTT112300.021.42384312-13
GTACTTT114800.021.26620714-15
Produced by FastQC (version 0.11.5)