##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937773_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2257616 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30679088029142 33.0 31.0 34.0 31.0 34.0 2 32.523028274073184 34.0 31.0 34.0 31.0 34.0 3 32.586737514262836 34.0 31.0 34.0 31.0 34.0 4 36.16928521059383 37.0 35.0 37.0 35.0 37.0 5 36.099150165484296 37.0 35.0 37.0 35.0 37.0 6 36.14736872878293 37.0 35.0 37.0 35.0 37.0 7 36.10589489089376 37.0 35.0 37.0 35.0 37.0 8 36.07146653815352 37.0 35.0 37.0 35.0 37.0 9 37.77973977859831 39.0 38.0 39.0 35.0 39.0 10-11 37.705281589074495 39.0 37.0 39.0 35.0 39.0 12-13 37.60557486304137 39.0 37.0 39.0 35.0 39.0 14-15 38.862267320926144 40.0 38.0 41.0 35.0 41.0 16-17 38.826278029567476 40.0 38.0 41.0 35.0 41.0 18-19 38.80663895011375 40.0 38.0 41.0 35.0 41.0 20-21 38.70721548748769 40.0 38.0 41.0 34.5 41.0 22-23 38.65726855231359 40.0 38.0 41.0 34.0 41.0 24-25 38.67636458104478 40.0 38.0 41.0 34.0 41.0 26-27 38.51001875429657 40.0 38.0 41.0 34.0 41.0 28-29 38.47639899788095 40.0 38.0 41.0 34.0 41.0 30-31 38.477268720632736 40.0 38.0 41.0 34.0 41.0 32-33 38.352199178248206 40.0 38.0 41.0 34.0 41.0 34-35 38.212456635672325 40.0 38.0 41.0 34.0 41.0 36-37 38.05099206419515 40.0 37.5 41.0 33.5 41.0 38-39 37.91441082097221 40.0 37.0 41.0 33.0 41.0 40-41 37.743160705806474 40.0 37.0 41.0 33.0 41.0 42-43 37.514912854976224 40.0 37.0 41.0 33.0 41.0 44-45 37.20582951219339 40.0 37.0 41.0 32.0 41.0 46-47 36.85220072855614 40.0 36.5 41.0 31.0 41.0 48-49 36.494718986754165 39.5 36.0 41.0 30.5 41.0 50-51 36.24409221940312 39.0 36.0 41.0 30.0 41.0 52-53 35.980360699073714 39.0 35.0 40.5 29.0 41.0 54-55 35.75321777485631 39.0 35.0 40.0 28.5 41.0 56-57 35.48578190445142 38.5 35.0 40.0 28.0 41.0 58-59 35.230075663886154 38.0 35.0 40.0 28.0 41.0 60-61 34.89676964550216 38.0 34.0 40.0 27.0 41.0 62-63 34.49560000460663 37.5 34.0 40.0 26.0 41.0 64-65 34.10320554957087 37.0 34.0 39.5 26.0 41.0 66-67 33.68511385461478 36.5 33.0 39.0 25.5 40.5 68-69 33.22371563631725 36.0 33.0 39.0 24.0 40.0 70-71 32.79982202464901 35.0 32.0 38.5 23.0 40.0 72-73 32.332322458735234 35.0 32.0 37.5 22.0 40.0 74-75 31.847200099574064 35.0 31.0 37.0 20.5 39.0 76-77 30.58686087447998 33.5 29.5 35.5 20.0 38.5 78-79 31.183252820674554 34.0 31.0 36.0 20.0 38.5 80-81 31.01297275533129 34.0 31.0 36.0 19.5 37.5 82-83 30.630174041998288 34.0 31.0 35.0 18.0 37.0 84-85 30.230841294533704 34.0 30.5 35.0 15.5 36.5 86-87 29.894662112600194 34.0 30.0 35.0 9.0 36.0 88-89 29.49745439436999 34.0 30.0 35.0 6.0 36.0 90-91 29.005990611335143 34.0 29.0 35.0 2.0 35.0 92-93 28.541871602610897 34.0 29.0 35.0 2.0 35.0 94-95 28.0534149740257 33.0 28.5 35.0 2.0 35.0 96-97 27.546441024514355 33.0 27.0 35.0 2.0 35.0 98-99 26.730608526870824 33.0 25.5 34.5 2.0 35.0 100-101 24.258230141884184 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16058906400722606 1101 2 0.171107880182106 1101 3 0.14132440076552655 1101 4 0.08361901814556205 1101 5 0.07100099264134485 1101 6 0.04464059382166852 1101 7 0.03410495828521931 1101 8 0.109500732493224 1101 9 0.11300957903894471 1101 10-11 0.16496585846402212 1101 12-13 0.16257235387272573 1101 14-15 0.23930648848381963 1101 16-17 0.19133521940252507 1101 18-19 0.17523331817633903 1101 20-21 0.15714060308879851 1101 22-23 0.1432272458745345 1101 24-25 0.09648076999518196 1101 26-27 0.04779927451230748 1101 28-29 0.12716298693785433 1101 30-31 0.20823107404514474 1101 32-33 0.18059567515431496 1101 34-35 0.1656797798114269 1101 36-37 0.11784985428246131 1101 38-39 0.18614158428297145 1101 40-41 0.12678352635253987 1101 42-43 0.10693265504698957 1101 44-45 0.2939601434177561 1101 46-47 0.20123803779082294 1101 48-49 0.21766067770985842 1101 50-51 0.2261424268531087 1101 52-53 0.20307405815718482 1101 54-55 0.2889293890525053 1101 56-57 0.24578585113186335 1101 58-59 0.14421323744772252 1101 60-61 0.2289689088686515 1101 62-63 0.162671194002165 1101 64-65 0.22053629741463254 1101 66-67 0.16811777383017557 1101 68-69 0.3872573127266534 1101 70-71 0.2430684582538234 1101 72-73 0.3097573600103303 1101 74-75 0.3585545030044486 1101 76-77 0.12734121076901062 1101 78-79 0.3366000998547065 1101 80-81 0.4861895375683005 1101 82-83 0.5802440955435202 1101 84-85 0.5432239742030802 1101 86-87 0.5427863701291109 1101 88-89 0.7127302472902279 1101 90-91 0.6622612430056556 1101 92-93 0.6016833852341641 1101 94-95 0.38394778238177096 1101 96-97 0.5517071169626853 1101 98-99 0.5295448710778139 1101 100-101 0.8911954818221695 1102 1 0.1837903260171032 1102 2 0.12931774847653088 1102 3 0.1658219690610494 1102 4 0.16167185852595622 1102 5 0.11949961281609234 1102 6 0.11865004357114373 1102 7 0.05787721417384262 1102 8 0.13099698164884188 1102 9 0.1288286840098678 1102 10-11 0.19170962718299478 1102 12-13 0.19208144318805154 1102 14-15 0.2537806316252116 1102 16-17 0.28418785026524773 1102 18-19 0.29056749211401467 1102 20-21 0.3198599897868206 1102 22-23 0.26272633436847315 1102 24-25 0.2752864457295061 1102 26-27 0.17916349627493133 1102 28-29 0.25705099183682023 1102 30-31 0.2640003649338851 1102 32-33 0.30952528077316543 1102 34-35 0.22200627245624105 1102 36-37 0.32816968747746955 1102 38-39 0.3477257260390232 1102 40-41 0.4105794372640119 1102 42-43 0.35634740930814246 1102 44-45 0.3378017001254534 1102 46-47 0.34215468371822055 1102 48-49 0.3596705792279735 1102 50-51 0.2971462665800644 1102 52-53 0.393829382223025 1102 54-55 0.3490924671110136 1102 56-57 0.3715857176241215 1102 58-59 0.44350176313724177 1102 60-61 0.31482901015800024 1102 62-63 0.3962961132416183 1102 64-65 0.5432430811774438 1102 66-67 0.4180087394381502 1102 68-69 0.3829563925162276 1102 70-71 0.4506129332520814 1102 72-73 0.35840899612386323 1102 74-75 0.49864346832553963 1102 76-77 0.36420015290707397 1102 78-79 0.3043031628093118 1102 80-81 0.44484251611511283 1102 82-83 0.41531689649682946 1102 84-85 0.4401383545444624 1102 86-87 0.4655161560876273 1102 88-89 0.5933073985855231 1102 90-91 0.6603281237669307 1102 92-93 0.7772182590760828 1102 94-95 0.8288622742861058 1102 96-97 0.8202441895754546 1102 98-99 0.6581269918281514 1102 100-101 1.2962563326674115 1103 1 0.15156896408032594 1103 2 0.16551897973384655 1103 3 0.14113072760620327 1103 4 0.13502136942220488 1103 5 0.11132270455609472 1103 6 0.11616675618964223 1103 7 0.121352116919617 1103 8 0.08031620210432777 1103 9 0.1562628721731798 1103 10-11 0.12819931634717818 1103 12-13 0.2449221162250339 1103 14-15 0.21277570281907998 1103 16-17 0.16196148398508825 1103 18-19 0.2533323446275091 1103 20-21 0.2409778467947845 1103 22-23 0.31703290233311776 1103 24-25 0.1759832769415084 1103 26-27 0.24108752467693506 1103 28-29 0.22571955322973025 1103 30-31 0.281640675392012 1103 32-33 0.3458846681660006 1103 34-35 0.2846479048164028 1103 36-37 0.27596636006121855 1103 38-39 0.36639995272633286 1103 40-41 0.36999704499902464 1103 42-43 0.23144183088158599 1103 44-45 0.3733160144359857 1103 46-47 0.2675356747670534 1103 48-49 0.243742131896326 1103 50-51 0.37219725422971806 1103 52-53 0.3298823716100401 1103 54-55 0.3886739906975194 1103 56-57 0.3347919909934589 1103 58-59 0.3033447400606022 1103 60-61 0.3915984704442863 1103 62-63 0.2907658416851788 1103 64-65 0.32416378594006545 1103 66-67 0.3856717110739041 1103 68-69 0.2818238057427962 1103 70-71 0.26741619646166015 1103 72-73 0.29699012313977136 1103 74-75 0.25124714321317754 1103 76-77 0.20375193369794786 1103 78-79 0.3974004736829002 1103 80-81 0.37608236579596976 1103 82-83 0.3532701200493449 1103 84-85 0.37299753420982995 1103 86-87 0.5094221877377372 1103 88-89 0.6834351509743897 1103 90-91 0.7827433331939808 1103 92-93 0.7657672827570146 1103 94-95 0.8570069847895603 1103 96-97 0.8957861694070068 1103 98-99 0.9949570707645883 1103 100-101 1.6959145265715527 1104 1 0.10886833806960539 1104 2 0.10726681877326172 1104 3 0.08492203920032892 1104 4 0.05217407006028907 1104 5 0.09953854727165634 1104 6 0.04331831342127401 1104 7 -0.0033640363092644066 1104 8 0.043253104521397745 1104 9 0.06874330017625851 1104 10-11 0.11174995726702264 1104 12-13 0.1190659195053172 1104 14-15 0.07885202338885478 1104 16-17 0.13314238140565493 1104 18-19 0.1383097946862577 1104 20-21 0.06647738003317016 1104 22-23 -0.005687014996695439 1104 24-25 -0.07004571384450742 1104 26-27 0.04765324735206633 1104 28-29 -0.0639802344553928 1104 30-31 -0.01805883118412055 1104 32-33 0.09065763645086378 1104 34-35 0.0719305864511739 1104 36-37 0.017514422021776 1104 38-39 0.09180235677720106 1104 40-41 0.16278023599291913 1104 42-43 0.16961053179422692 1104 44-45 0.17463931338607352 1104 46-47 0.14687137114038507 1104 48-49 0.09392234344549166 1104 50-51 -0.009153027732928365 1104 52-53 0.0926151854608861 1104 54-55 0.18621904703400105 1104 56-57 0.07655433387638766 1104 58-59 0.08851203353130899 1104 60-61 0.09102925063552192 1104 62-63 0.06181585959509306 1104 64-65 0.11398265690638709 1104 66-67 0.23431272163135475 1104 68-69 0.2521439578244795 1104 70-71 0.27795579980136864 1104 72-73 0.26119184349220603 1104 74-75 0.412646810676776 1104 76-77 0.28924864204979883 1104 78-79 0.3151452277668696 1104 80-81 0.28464494522434336 1104 82-83 0.09258586630831189 1104 84-85 0.20187684239891723 1104 86-87 0.14335500752119046 1104 88-89 0.07179772222818315 1104 90-91 0.16353717459643846 1104 92-93 0.30968004032742513 1104 94-95 0.6405624718212088 1104 96-97 0.46987882120111024 1104 98-99 0.4625421877171334 1104 100-101 0.7378009713069922 1105 1 0.14298805889016108 1105 2 0.20829221234010475 1105 3 0.13550189426005232 1105 4 0.11398013575158217 1105 5 0.09440245917768664 1105 6 0.10723475194325971 1105 7 0.12888581016636635 1105 8 0.10825457758153334 1105 9 0.12442442052002178 1105 10-11 0.18756059635401812 1105 12-13 0.12261563514667273 1105 14-15 0.12995504971213734 1105 16-17 0.22087928015253766 1105 18-19 0.25505354136634395 1105 20-21 0.20751805141694746 1105 22-23 0.2534668238864555 1105 24-25 0.1645121663639202 1105 26-27 0.1925763856721332 1105 28-29 0.17413620528262896 1105 30-31 0.2561904194345317 1105 32-33 0.2159813365789418 1105 34-35 0.37433877721827 1105 36-37 0.3279083674958869 1105 38-39 0.33907277358412813 1105 40-41 0.35813177778664596 1105 42-43 0.5080832696796094 1105 44-45 0.3747085168115305 1105 46-47 0.4048564349186421 1105 48-49 0.5071784475247867 1105 50-51 0.5156982982146516 1105 52-53 0.6079126247683178 1105 54-55 0.49230242610681785 1105 56-57 0.5246622668994121 1105 58-59 0.5354633517957623 1105 60-61 0.6306070323561954 1105 62-63 0.49410318204712667 1105 64-65 0.4873365191152885 1105 66-67 0.5552380797673777 1105 68-69 0.5682880888820634 1105 70-71 0.47557303521296745 1105 72-73 0.49021262054939285 1105 74-75 0.6023308019677529 1105 76-77 0.544168745810719 1105 78-79 0.6292124269461574 1105 80-81 0.6719107963739681 1105 82-83 0.6156333154269369 1105 84-85 0.6660248249698242 1105 86-87 0.8229594535649021 1105 88-89 0.8017048227096808 1105 90-91 0.8812377747095255 1105 92-93 0.754666909917109 1105 94-95 1.113528087336487 1105 96-97 1.2829729163027572 1105 98-99 1.405846507569617 1105 100-101 1.6510222525763574 1106 1 -0.16535816496621436 1106 2 -0.09641775195944291 1106 3 -0.08581482300752441 1106 4 -0.09535918168934643 1106 5 -0.12263699671622419 1106 6 -0.047010662255836166 1106 7 -0.03284443764352574 1106 8 -0.04774787791892976 1106 9 -0.31399711303998146 1106 10-11 -0.2983838208213285 1106 12-13 -0.24756750639874525 1106 14-15 -0.25277162805298303 1106 16-17 0.015762436692398296 1106 18-19 -0.13420682669386252 1106 20-21 -0.11061877566464773 1106 22-23 -0.15295737706131263 1106 24-25 -0.14379945492852642 1106 26-27 -0.0134927758821064 1106 28-29 -0.03721273081668386 1106 30-31 -0.017531453700499355 1106 32-33 -0.12921362750915222 1106 34-35 -0.21801076677547826 1106 36-37 -0.05417645406808447 1106 38-39 0.01229630571986462 1106 40-41 0.012191790593867324 1106 42-43 -0.22631172157789337 1106 44-45 -0.05476270047676479 1106 46-47 0.18123181207590733 1106 48-49 0.13181271764778302 1106 50-51 0.10965173512499149 1106 52-53 0.11253836813605744 1106 54-55 0.17871070987518323 1106 56-57 -0.02646985636944521 1106 58-59 0.22358649164095112 1106 60-61 0.16877398075608596 1106 62-63 0.11531510908100273 1106 64-65 -0.1782746625537186 1106 66-67 0.027811275937850155 1106 68-69 -0.007976495991833588 1106 70-71 0.09109796678746562 1106 72-73 0.18397768708243945 1106 74-75 0.04718510167807821 1106 76-77 0.024527518863607156 1106 78-79 -0.0033935690373816385 1106 80-81 0.02628291576391817 1106 82-83 -0.1406728950497822 1106 84-85 0.07790288126264144 1106 86-87 0.29252382864473603 1106 88-89 0.06456416072418136 1106 90-91 -0.08692647810791598 1106 92-93 -0.05811121887021642 1106 94-95 -0.15262255257599122 1106 96-97 -0.4402210968556126 1106 98-99 -0.28553568116643646 1106 100-101 0.28191969356723945 1107 1 0.09360714406123805 1107 2 0.12954745962019842 1107 3 0.09287418812866832 1107 4 0.11960555244056792 1107 5 0.11564090038690722 1107 6 0.07964878704394351 1107 7 0.039441807932945494 1107 8 0.03120397530243224 1107 9 0.04245204034330641 1107 10-11 0.16538550910712502 1107 12-13 0.11657468373837787 1107 14-15 0.10801450872902052 1107 16-17 0.12931081819119328 1107 18-19 0.16012725106948267 1107 20-21 0.040255784985923526 1107 22-23 0.14238577491676807 1107 24-25 -0.004354987494110674 1107 26-27 0.07000441659776868 1107 28-29 0.09219583666947528 1107 30-31 0.18736678899080061 1107 32-33 0.22046357108457215 1107 34-35 0.27826530329261345 1107 36-37 0.2535473521629612 1107 38-39 0.36301773093179435 1107 40-41 0.30139128137348337 1107 42-43 0.27794906119938645 1107 44-45 0.2788569692063305 1107 46-47 0.18801533601091336 1107 48-49 0.31042552816253277 1107 50-51 0.372236629291784 1107 52-53 0.40332272059985996 1107 54-55 0.35843960920004037 1107 56-57 0.4023051446650996 1107 58-59 0.42280973476609773 1107 60-61 0.3329727484641083 1107 62-63 0.4641092704002787 1107 64-65 0.4537585435635094 1107 66-67 0.5128581554500968 1107 68-69 0.47962683093842173 1107 70-71 0.45878543255280135 1107 72-73 0.37467980962015446 1107 74-75 0.4796153964643537 1107 76-77 0.2780415355507664 1107 78-79 0.47583425279955804 1107 80-81 0.6244758152454004 1107 82-83 0.6246898176834428 1107 84-85 0.6259004846696641 1107 86-87 0.7786834597135872 1107 88-89 0.6698045608920253 1107 90-91 0.4940015853906772 1107 92-93 0.575119282558795 1107 94-95 0.6970945818178755 1107 96-97 0.7745738823132484 1107 98-99 0.9206016837471047 1107 100-101 1.414896341247161 1108 1 0.1162839817866157 1108 2 0.1479339598513647 1108 3 0.11300363796685531 1108 4 0.0802111927549447 1108 5 -0.04126617312211067 1108 6 0.03783648930931349 1108 7 0.04455507654135715 1108 8 0.11114849680035377 1108 9 0.10227173521154498 1108 10-11 0.023517732870416808 1108 12-13 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0.22535151093293848 2316 72-73 0.23381977558262435 2316 74-75 0.19247269007829715 2316 76-77 0.17215791644954592 2316 78-79 0.2176958982597661 2316 80-81 0.1307117027339224 2316 82-83 0.13686426675109686 2316 84-85 0.12014115617395049 2316 86-87 0.3734722159364914 2316 88-89 0.44320536626834794 2316 90-91 0.2949397952039412 2316 92-93 0.443405998539685 2316 94-95 0.43823845740666556 2316 96-97 0.5325954003337152 2316 98-99 0.3863149811668194 2316 100-101 0.6553835872219516 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 22.0 5 27.0 6 90.0 7 210.0 8 518.0 9 999.0 10 1699.0 11 2684.0 12 4247.0 13 6330.0 14 8146.0 15 11541.0 16 16335.0 17 23666.0 18 22316.0 19 13460.0 20 10891.0 21 10978.0 22 11348.0 23 12158.0 24 13801.0 25 16123.0 26 19743.0 27 24399.0 28 30716.0 29 38669.0 30 48870.0 31 62163.0 32 79433.0 33 106171.0 34 147353.0 35 215319.0 36 335178.0 37 517761.0 38 408574.0 39 35669.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01035004750493 19.788239694266792 15.03056800294956 22.170842255278718 2 15.615100176469337 20.63030205313924 40.236204917045235 23.518392853346185 3 19.56027951609131 24.68648344093947 29.935073103663335 25.818163939305887 4 12.984892027696473 16.975428948058482 36.673818753942214 33.36586027030283 5 13.426817221353485 37.906601755093185 34.02174153794359 14.644839485609742 6 32.01124549081864 36.56534149297312 16.960058752241302 14.463354263966947 7 28.55941843076945 33.40953465957009 20.027143677224117 18.00390323243634 8 26.5044631150736 35.82274399189233 19.05492342364689 18.617869469387177 9 26.580516792935555 16.837983075952685 20.290651731738258 36.2908483993735 10-11 25.00259122897782 27.420141423519322 26.60370497019865 20.9735623773042 12-13 25.749161077355854 24.59747106157075 27.75110259828479 21.9022652627886 14-15 22.652634460421968 25.842769540967108 26.329344760136358 25.175251238474566 16-17 21.668188921410906 29.414258226376848 27.002842821808493 21.914710030403754 18-19 21.50292609549188 27.40995368565779 30.081355731001196 21.00576448784913 20-21 22.190721329832282 26.424937367337463 30.455353630922637 20.928987671907613 22-23 22.0737767385142 26.329193431112014 30.30466452706137 21.292365303312415 24-25 21.760941630463286 26.53387467133472 30.469973635906193 21.235210062295803 26-27 21.425087936810407 26.890901657389062 30.35391327111646 21.330097134684067 28-29 21.396992087541566 26.932745210688086 30.462167658363228 21.20809504340712 30-31 21.51634733276164 26.7335100389083 30.358860851446835 21.391281776883226 32-33 21.47341807026842 26.73268620484144 30.298175352789414 21.49572037210073 34-35 21.803885160275264 26.833349869951313 30.194594652057745 21.168170317715678 36-37 21.85881096533959 26.52920385302209 30.103523447087387 21.508461734550938 38-39 21.510810518706457 26.947386092231806 30.12168588457913 21.420117504482604 40-41 21.875334285518758 26.80565118874422 30.10039140889262 21.2186231168444 42-43 21.687751282022884 26.787887514260085 29.907805110370262 21.616556093346773 44-45 21.69354752978363 27.078209934727603 29.77860273846394 21.449639797024826 46-47 21.930279551526922 27.40691951155555 29.06603691681845 21.596764020099076 48-49 21.981018915528594 27.496726635530578 28.894528564645185 21.627725884295646 50-51 22.223819285476363 27.729294973104373 28.50323084173748 21.543654899681787 52-53 22.317672038339584 27.7895539738959 28.350389315802143 21.542384671962374 54-55 22.124754608401073 27.861957037866492 28.430255632490205 21.583032721242233 56-57 22.06739321478941 28.144511732730454 28.346140353363904 21.441954699116238 58-59 22.121476814480406 27.673705359990365 28.465026824756734 21.739791000772495 60-61 22.007728506530782 27.865567040630467 28.686211472633076 21.44049298020567 62-63 21.973466701157328 27.88113656175364 28.789971368027157 21.35542536906188 64-65 21.9719385404781 27.688101076533833 28.647320890709494 21.69263949227858 66-67 21.823596218311707 27.97058932962913 28.505910659740184 21.699903792318977 68-69 21.904743765104428 27.804307729923956 28.58951655197341 21.701431952998206 70-71 21.930899674701102 27.913781617422977 28.426712071494887 21.72860663638103 72-73 22.052643142146405 27.908111919830475 28.18397814331578 21.855266794707337 74-75 21.918475063961278 28.251925925400954 27.960313002742716 21.869286007895052 76-77 22.025955698400438 27.979913324498057 28.220631852361073 21.773499124740432 78-79 22.109716621427207 28.047993990120553 28.024562193039028 21.817727195413212 80-81 21.97787400514525 28.297106328091225 27.963745827456925 21.761273839306597 82-83 22.044020358657335 28.30624980482718 27.863703036988824 21.78602679952667 84-85 21.990258729249636 28.23905446952553 27.82784545256025 21.942841348664587 86-87 21.95388852665821 28.1563162202961 27.956592263697633 21.933202989348054 88-89 22.0230322605793 28.14807744098199 27.848779420415166 21.98011087802354 90-91 22.00164792082892 28.22476399735978 27.88370640429877 21.889881677512527 92-93 21.98982576134591 27.9546165790612 27.922546620484155 22.133011039108737 94-95 21.854225873664966 27.986956151976244 27.91136756649492 22.24745040786387 96-97 21.938363300047484 27.95351379508296 27.874204470556553 22.23391843431301 98-99 21.755909470434375 28.014577395071328 27.670185882827152 22.559327251667145 100-101 21.546041301513092 28.1841151468341 27.986026833070003 22.283816718582802 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 590.0 1 438.0 2 351.0 3 449.5 4 412.0 5 391.5 6 753.5 7 1646.0 8 2744.0 9 3407.5 10 3362.5 11 3221.0 12 3490.5 13 3804.0 14 4059.0 15 4328.5 16 4467.0 17 4615.5 18 4741.0 19 4906.0 20 5334.5 21 5998.5 22 7014.0 23 8440.0 24 9946.0 25 12103.5 26 15073.5 27 18167.5 28 20827.0 29 23719.0 30 27536.5 31 31931.5 32 36842.0 33 41551.5 34 46805.5 35 50943.0 36 56392.5 37 62712.0 38 66349.5 39 68514.5 40 69613.5 41 72852.0 42 75643.5 43 78656.5 44 83298.0 45 86055.5 46 85831.0 47 85954.5 48 87583.0 49 85879.5 50 83648.0 51 80498.0 52 75350.0 53 72838.5 54 71333.0 55 65225.0 56 56876.0 57 50465.0 58 45568.5 59 40133.5 60 32659.0 61 26002.5 62 20561.0 63 15111.5 64 11005.5 65 8265.5 66 6019.5 67 4744.5 68 3576.0 69 2519.5 70 1874.5 71 1198.5 72 815.0 73 623.0 74 433.5 75 298.5 76 214.0 77 132.0 78 68.0 79 37.5 80 24.5 81 16.0 82 10.0 83 9.0 84 4.0 85 1.5 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0446488685409742 2 0.0 3 0.0 4 0.0 5 0.008238779314108333 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004761660087455086 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.543560995315413E-4 22-23 0.0015281606792297716 24-25 0.0 26-27 0.001284540860801837 28-29 0.0016388969603333783 30-31 0.0 32-33 6.644176866216399E-5 34-35 0.0 36-37 5.093868930765906E-4 38-39 0.0 40-41 1.1073628110360662E-4 42-43 0.01953387998667621 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 6.644176866216399E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.644176866216399E-5 84-85 2.2147256220721325E-4 86-87 0.0 88-89 0.0 90-91 0.01001055981176604 92-93 0.0022811673907342967 94-95 0.0 96-97 0.0 98-99 4.8723963685586914E-4 100-101 0.012823261351797649 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2257616.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.904897487098275 #Duplication Level Percentage of deduplicated Percentage of total 1 74.402542164413 37.874537816589516 2 13.933245677954694 14.185408857976778 3 4.803527404015813 7.335692102336767 4 2.184578487116669 4.448229757567771 5 1.1801741069529883 3.003832096568481 6 0.7372134869093179 2.2516666186335055 7 0.48550035894169674 1.7300042201313541 8 0.33988066729630395 1.3841272421251927 9 0.2541036578560094 1.1641608588831165 >10 1.4882132546664806 14.12158031086512 >50 0.12809260470461747 4.491853126136204 >100 0.059783989262888255 5.4710583384716704 >500 0.0024454421518531845 0.872950936630638 >1k 4.366860985452048E-4 0.3550934112996601 >5k 8.733721970904095E-5 0.30876721440793026 >10k+ 1.746744394180819E-4 1.0010370913763798 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11973 0.5303381974613929 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10543 0.4669970446701299 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6945 0.3076253889058192 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2847 0.12610647692078725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.429451244144265E-5 7 0.0 0.0 0.0 0.0 4.429451244144265E-5 8 0.0 0.0 0.0 0.0 4.429451244144265E-5 9 0.0 0.0 0.0 4.429451244144265E-5 4.429451244144265E-5 10-11 0.0 0.0 0.0 8.85890248828853E-5 4.429451244144265E-5 12-13 0.0 0.0 0.0 8.85890248828853E-5 8.85890248828853E-5 14-15 0.0 0.0 0.0 8.85890248828853E-5 8.85890248828853E-5 16-17 0.0 0.0 0.0 8.85890248828853E-5 8.85890248828853E-5 18-19 0.0 0.0 0.0 8.85890248828853E-5 8.85890248828853E-5 20-21 0.0 0.0 0.0 1.1073628110360664E-4 8.85890248828853E-5 22-23 0.0 0.0 0.0 2.4361981842793462E-4 8.85890248828853E-5 24-25 0.0 0.0 0.0 3.543560995315412E-4 8.85890248828853E-5 26-27 0.0 0.0 0.0 5.979759179594758E-4 8.85890248828853E-5 28-29 4.429451244144265E-5 0.0 0.0 0.001550307935450493 8.85890248828853E-5 30-31 4.429451244144265E-5 0.0 0.0 0.003942211607288396 8.85890248828853E-5 32-33 4.429451244144265E-5 0.0 0.0 0.00699853296574794 8.85890248828853E-5 34-35 4.429451244144265E-5 0.0 0.0 0.009523320174910171 8.85890248828853E-5 36-37 4.429451244144265E-5 0.0 0.0 0.01300043940156342 8.85890248828853E-5 38-39 4.429451244144265E-5 0.0 0.0 0.017607068695473453 8.85890248828853E-5 40-41 4.429451244144265E-5 0.0 0.0 0.023985478487041197 8.85890248828853E-5 42-43 4.429451244144265E-5 0.0 0.0 0.029433703517338643 8.85890248828853E-5 44-45 4.429451244144265E-5 0.0 0.0 0.032445730363356746 8.85890248828853E-5 46-47 8.85890248828853E-5 0.0 0.0 0.036830887095059564 8.85890248828853E-5 48-49 8.85890248828853E-5 0.0 0.0 0.04332003316773091 8.85890248828853E-5 50-51 8.85890248828853E-5 0.0 0.0 0.049875621009064425 8.85890248828853E-5 52-53 8.85890248828853E-5 0.0 0.0 0.06380624517189815 2.2147256220721328E-4 54-55 8.85890248828853E-5 0.0 0.0 0.07452551718272726 2.2147256220721328E-4 56-57 8.85890248828853E-5 0.0 0.0 0.09873246823197568 2.4361981842793462E-4 58-59 8.85890248828853E-5 4.429451244144265E-5 0.0 0.14273906634254896 2.6576707464865594E-4 60-61 8.85890248828853E-5 4.429451244144265E-5 0.0 0.18067731624864458 2.6576707464865594E-4 62-63 8.85890248828853E-5 4.429451244144265E-5 0.0 0.20410911333016774 2.6576707464865594E-4 64-65 8.85890248828853E-5 4.429451244144265E-5 0.0 0.24313257879107872 3.100615870900986E-4 66-67 8.85890248828853E-5 4.429451244144265E-5 0.0 0.3113239806946797 3.100615870900986E-4 68-69 8.85890248828853E-5 4.429451244144265E-5 0.0 0.4064685934188985 3.100615870900986E-4 70-71 8.85890248828853E-5 4.429451244144265E-5 0.0 0.49629786465014425 3.100615870900986E-4 72-73 8.85890248828853E-5 4.429451244144265E-5 0.0 0.5993047533327192 3.543560995315412E-4 74-75 8.85890248828853E-5 4.429451244144265E-5 0.0 0.7247911070793261 3.986506119729839E-4 76-77 8.85890248828853E-5 4.429451244144265E-5 0.0 0.821043082614581 3.986506119729839E-4 78-79 8.85890248828853E-5 4.429451244144265E-5 0.0 0.9453334845252692 3.986506119729839E-4 80-81 8.85890248828853E-5 4.429451244144265E-5 0.0 1.1068755713992104 3.986506119729839E-4 82-83 8.85890248828853E-5 4.429451244144265E-5 0.0 1.2571447048568047 4.4294512441442655E-4 84-85 8.85890248828853E-5 4.429451244144265E-5 0.0 1.4381542299487602 4.4294512441442655E-4 86-87 8.85890248828853E-5 4.429451244144265E-5 0.0 1.667599804395433 4.6509238063514787E-4 88-89 8.85890248828853E-5 4.429451244144265E-5 0.0 1.9716594850497162 4.872396368558692E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5945 0.0 52.425896 1 GTATCAA 10375 0.0 41.672268 1 ATCAACG 13260 0.0 32.31105 3 TCAACGC 13560 0.0 31.5962 4 TATCAAC 13830 0.0 31.151083 2 CAACGCA 13790 0.0 31.138105 5 AACGCAG 14215 0.0 30.239216 6 TGGTATC 2690 0.0 26.663227 2 ACGCAGA 16295 0.0 26.206137 7 CGCAGAG 16410 0.0 25.964592 8 GTACATG 10240 0.0 25.101007 1 TACATGG 10315 0.0 24.313793 2 GCAGAGT 17610 0.0 23.84463 9 ACATGGG 10090 0.0 23.679081 3 GTACGCT 125 2.257035E-4 22.799707 3 GAGTACT 10950 0.0 22.40546 12-13 GTGGTAT 3375 0.0 22.382944 1 GTACCTG 1740 0.0 22.117172 1 AGTACTT 11230 0.0 21.423843 12-13 GTACTTT 11480 0.0 21.266207 14-15 >>END_MODULE