FastQCFastQC Report
Thu 26 May 2016
SRR937772_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937772_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886865
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91810.48657429121850265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72970.38672613038028686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46450.24617553455069655No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23920.12677112565021875No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.1005901323094127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40800.054.7646941
GTATCAA76350.044.769561
ATCAACG97150.035.123243
TCAACGC98350.034.7941064
CAACGCA99050.034.5482145
TATCAAC99950.034.281942
AACGCAG103400.033.127546
TGGTATC17750.032.396642
ACGCAGA117300.028.8366587
CGCAGAG116700.028.822088
GTGGTAT21700.028.2613541
GTACATG86400.027.4567931
TACATGG84700.027.3248942
ACATGGG84050.026.2922763
GCAGAGT126800.025.7769879
GAGTACT76700.023.43973512-13
CATGGGG37200.022.2307364
CAGAGTA121450.022.15653610-11
CATGGGA46150.021.8329144
AGAGTAC118650.021.67854910-11
Produced by FastQC (version 0.11.5)