FastQCFastQC Report
Thu 26 May 2016
SRR937772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886865
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102590.5437060945006664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86720.4595983284442713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58510.3100910769980894No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23900.12666512972576205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46200.054.3096851
GTATCAA83450.041.7410051
ATCAACG107750.032.0043453
TCAACGC108850.031.6809224
CAACGCA110650.031.1655525
TATCAAC111700.030.9576382
AACGCAG115150.029.9468236
TGGTATC20000.028.262242
ACGCAGA133400.025.7458117
CGCAGAG133150.025.6514478
GTACATG87500.024.8195231
TACATGG86800.024.3517362
GCAGAGT142750.023.6268799
ACATGGG85700.023.611223
GAGTACT89600.022.42624312-13
GTACCTG13750.022.1188531
TAAGGTG9250.022.0808775
ACTACCG1100.002878197221.590715
GTGGTAT27350.021.3714051
AGTACTT92100.021.14698212-13
Produced by FastQC (version 0.11.5)