##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937772_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1886865 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.305183995675364 33.0 31.0 34.0 31.0 34.0 2 32.53731189035781 34.0 31.0 34.0 31.0 34.0 3 32.63792481179099 34.0 31.0 34.0 31.0 34.0 4 36.224972109822374 37.0 35.0 37.0 35.0 37.0 5 36.08096021707966 37.0 35.0 37.0 35.0 37.0 6 36.174627755562796 37.0 36.0 37.0 35.0 37.0 7 36.13941961931564 37.0 36.0 37.0 35.0 37.0 8 36.13467365179809 37.0 36.0 37.0 35.0 37.0 9 37.8192122912874 39.0 38.0 39.0 35.0 39.0 10-11 37.773095584474774 39.0 37.5 39.0 35.0 39.0 12-13 37.69750061610131 39.0 37.0 39.0 35.0 39.0 14-15 38.9679137087179 40.0 38.0 41.0 36.0 41.0 16-17 38.9415164836912 40.0 38.0 41.0 36.0 41.0 18-19 38.928830626462414 40.0 38.0 41.0 35.0 41.0 20-21 38.853859974083996 40.0 38.0 41.0 35.0 41.0 22-23 38.710216947158386 40.0 38.0 41.0 34.5 41.0 24-25 38.65991552124821 40.0 38.0 41.0 34.5 41.0 26-27 38.59572200448893 40.0 38.0 41.0 34.0 41.0 28-29 38.48546530885888 40.0 38.0 41.0 34.0 41.0 30-31 38.54110680944318 40.0 38.0 41.0 34.5 41.0 32-33 38.45883409782894 40.0 38.0 41.0 34.0 41.0 34-35 38.33585418140673 40.0 38.0 41.0 34.0 41.0 36-37 38.183078015650295 40.0 38.0 41.0 34.0 41.0 38-39 38.05079881178568 40.0 38.0 41.0 34.0 41.0 40-41 37.8760785747788 40.0 37.0 41.0 33.0 41.0 42-43 37.672898167065476 40.0 37.0 41.0 33.0 41.0 44-45 37.37538827632078 40.0 37.0 41.0 33.0 41.0 46-47 37.003526749396485 40.0 37.0 41.0 31.5 41.0 48-49 36.65757804612413 40.0 36.0 41.0 31.0 41.0 50-51 36.40515988160256 39.5 36.0 41.0 30.0 41.0 52-53 36.176326605242025 39.0 36.0 41.0 30.0 41.0 54-55 35.96659564939728 39.0 35.0 40.5 29.5 41.0 56-57 35.69413948533679 39.0 35.0 40.0 29.0 41.0 58-59 35.44229078391936 38.5 35.0 40.0 28.0 41.0 60-61 35.105574590656985 38.0 34.5 40.0 28.0 41.0 62-63 34.744823291544435 38.0 34.0 40.0 27.0 41.0 64-65 34.34727656721599 37.0 34.0 40.0 26.0 41.0 66-67 33.928110914135345 36.5 33.5 39.0 26.0 41.0 68-69 33.48014510842059 36.0 33.0 39.0 25.0 40.0 70-71 33.06928768088867 35.5 33.0 38.5 24.5 40.0 72-73 32.62424179790287 35.0 32.0 38.0 23.5 40.0 74-75 32.13408378447849 35.0 32.0 37.0 22.0 39.0 76-77 30.911871013559526 34.0 30.5 36.0 20.5 38.5 78-79 31.45523898106118 34.5 31.0 36.0 21.5 38.5 80-81 31.249176809151688 34.5 31.0 36.0 20.0 37.5 82-83 30.904019365455397 34.0 31.0 35.5 19.5 37.0 84-85 30.51415973055836 34.0 31.0 35.0 18.0 36.5 86-87 30.105125697916915 34.0 30.5 35.0 14.0 36.0 88-89 29.681827528731517 34.0 30.0 35.0 7.5 36.0 90-91 29.18580582076619 34.0 29.0 35.0 3.5 35.0 92-93 28.746240192064615 34.0 29.0 35.0 2.0 35.0 94-95 28.301556550150647 34.0 29.0 35.0 2.0 35.0 96-97 27.796512734085375 33.0 28.0 35.0 2.0 35.0 98-99 27.02244066215654 33.0 26.5 35.0 2.0 35.0 100-101 24.608732209246554 31.0 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18462305791373268 1101 2 0.162077157491197 1101 3 0.15265263015658093 1101 4 0.06425309332836804 1101 5 0.006064156624205452 1101 6 0.044046807427136514 1101 7 0.04110478492713554 1101 8 0.07365279449953732 1101 9 0.251846751299297 1101 10-11 0.1770002341510306 1101 12-13 0.1694156585602684 1101 14-15 0.15326592815433315 1101 16-17 0.06503249741959394 1101 18-19 0.1612322850631287 1101 20-21 0.23926059252628562 1101 22-23 0.1934768697189284 1101 24-25 0.1925755248057186 1101 26-27 0.13401275431189674 1101 28-29 0.2211304801232643 1101 30-31 0.2151315381586727 1101 32-33 0.3269366158087692 1101 34-35 0.2375436680791907 1101 36-37 0.31771495442520603 1101 38-39 0.375840732528367 1101 40-41 0.21006403088772885 1101 42-43 0.1416796606321995 1101 44-45 0.2980634849175061 1101 46-47 0.22729286934927018 1101 48-49 0.25433623342615874 1101 50-51 0.29837421919064155 1101 52-53 0.1711895487118582 1101 54-55 0.24938357215528129 1101 56-57 0.39669654055614245 1101 58-59 0.3906436663986028 1101 60-61 0.3599869267895244 1101 62-63 0.4150119718686227 1101 64-65 0.3663726483140195 1101 66-67 0.3855852147630401 1101 68-69 0.4371971053487229 1101 70-71 0.36022147380528224 1101 72-73 0.31066606723906887 1101 74-75 0.46061730224156605 1101 76-77 0.16142208309894102 1101 78-79 0.27639474518213447 1101 80-81 0.3940931841055004 1101 82-83 0.32786991444482183 1101 84-85 0.3596272928894031 1101 86-87 0.43542075598716323 1101 88-89 0.4727001133804585 1101 90-91 0.47419779316730626 1101 92-93 0.6852691535769004 1101 94-95 0.6166811629332045 1101 96-97 0.75952611265539 1101 98-99 0.8323156818401323 1101 100-101 1.6099759448453668 1102 1 0.1367367639488819 1102 2 0.17900864681639206 1102 3 0.1125809447300199 1102 4 0.061483180926558134 1102 5 -0.011182662773762786 1102 6 0.04572193749834952 1102 7 0.037939588630756305 1102 8 0.08946005508659027 1102 9 0.21208981091754708 1102 10-11 0.06245682897625926 1102 12-13 0.07323052841104527 1102 14-15 0.0680078528183401 1102 16-17 0.14428243306652178 1102 18-19 0.1594351766613542 1102 20-21 0.11383976241060623 1102 22-23 0.20058934719535415 1102 24-25 0.1025777479119867 1102 26-27 0.07563583639521454 1102 28-29 -0.07012978711750861 1102 30-31 0.05851360432143338 1102 32-33 0.07295667056738608 1102 34-35 0.09822978887576284 1102 36-37 0.12280391767764343 1102 38-39 0.08073839163647278 1102 40-41 0.10098176425537986 1102 42-43 0.1294108441827717 1102 44-45 0.06268167616704545 1102 46-47 0.1278738996225215 1102 48-49 0.19434668592577964 1102 50-51 0.15415312542236137 1102 52-53 0.07105645484997325 1102 54-55 0.14004330819066269 1102 56-57 0.0841008884839809 1102 58-59 0.2446078158849474 1102 60-61 0.1509750585222278 1102 62-63 0.2992866151965714 1102 64-65 0.3031641794492046 1102 66-67 0.278404484024108 1102 68-69 0.2553508155960742 1102 70-71 0.16535177903386966 1102 72-73 0.10717783800171077 1102 74-75 -0.019096268067102073 1102 76-77 0.024326337422298394 1102 78-79 0.32332500594860036 1102 80-81 0.17350673257311655 1102 82-83 0.2527210248278955 1102 84-85 0.3018597791022479 1102 86-87 0.3499803469356131 1102 88-89 0.2653552822975378 1102 90-91 0.3085674060348005 1102 92-93 0.5248075860139672 1102 94-95 0.45467521904908637 1102 96-97 0.7886363241234946 1102 98-99 1.0197876004978426 1102 100-101 1.686488144628516 1103 1 0.2379079682657803 1103 2 0.2304189975644917 1103 3 0.08241476862013286 1103 4 0.07373483655569402 1103 5 0.020258960969187 1103 6 0.05071641873455235 1103 7 0.06452776621230782 1103 8 0.06644987603336716 1103 9 0.24172863848684045 1103 10-11 0.17692825404371604 1103 12-13 0.18717920390773202 1103 14-15 0.18770677359341903 1103 16-17 0.11683118214672561 1103 18-19 0.1044391624352059 1103 20-21 0.11246926940103918 1103 22-23 0.16573835381786495 1103 24-25 0.11335160123550736 1103 26-27 0.12325663600277181 1103 28-29 0.1312085131032461 1103 30-31 0.19032621163073316 1103 32-33 0.22370323666991254 1103 34-35 0.2802646797268835 1103 36-37 0.22963338787631926 1103 38-39 0.14936268646110307 1103 40-41 0.1278504789966135 1103 42-43 0.2977178100890754 1103 44-45 0.14252592438877798 1103 46-47 0.2481875493649781 1103 48-49 0.20277966360544752 1103 50-51 0.2438468340316362 1103 52-53 0.29056528487205213 1103 54-55 0.13072272933366236 1103 56-57 0.33351681931547006 1103 58-59 0.2217570677858589 1103 60-61 0.12842937542190214 1103 62-63 0.21513466523336433 1103 64-65 0.4848388176635794 1103 66-67 0.5045634244214554 1103 68-69 0.2257211859664423 1103 70-71 0.2342980017466516 1103 72-73 0.2734842520316292 1103 74-75 0.2089175901619953 1103 76-77 0.23955812550176958 1103 78-79 0.3222779738171333 1103 80-81 0.4528175002944792 1103 82-83 0.44156085803859213 1103 84-85 0.5226900464556898 1103 86-87 0.5737738838500093 1103 88-89 0.7341114142573097 1103 90-91 0.8246116787843754 1103 92-93 0.9571797346526729 1103 94-95 0.9095608579992955 1103 96-97 1.1446180508833699 1103 98-99 1.5356095897055937 1103 100-101 2.20131215738299 1104 1 0.1445436368919104 1104 2 0.237399178091934 1104 3 0.10797811383872613 1104 4 0.08224885794916048 1104 5 -0.012882039962356373 1104 6 0.002570278231516454 1104 7 0.061007159742878514 1104 8 0.023672178561390922 1104 9 0.1182484258370664 1104 10-11 0.12105308138622917 1104 12-13 0.0900178133324232 1104 14-15 0.11787967199051508 1104 16-17 0.0680881687517143 1104 18-19 0.07798904037038312 1104 20-21 0.163682288578066 1104 22-23 0.19742742580399408 1104 24-25 0.06552066101514953 1104 26-27 0.03648621423668175 1104 28-29 -0.0026723050522932112 1104 30-31 0.1379138252634462 1104 32-33 0.09900418267229583 1104 34-35 0.0899160320766299 1104 36-37 0.02092964401971642 1104 38-39 -0.1812215784195672 1104 40-41 -0.027141530575839568 1104 42-43 0.28429349027923934 1104 44-45 0.16325365558918747 1104 46-47 0.19336980338830045 1104 48-49 0.12649336538181188 1104 50-51 0.13260984357196293 1104 52-53 0.1464015092143498 1104 54-55 0.046417695765832434 1104 56-57 0.174569748871221 1104 58-59 0.2087744299007852 1104 60-61 0.1970211729870286 1104 62-63 0.1531068633638455 1104 64-65 0.10075181435497882 1104 66-67 0.2573966132043424 1104 68-69 0.19650432817196162 1104 70-71 0.2281945598398707 1104 72-73 0.3764356911116522 1104 74-75 0.2576025826132522 1104 76-77 -0.0458278241946779 1104 78-79 0.10552558070415685 1104 80-81 0.11291836438493874 1104 82-83 0.09481966758534455 1104 84-85 0.19855895526042033 1104 86-87 0.18739555517441175 1104 88-89 0.44767245469931183 1104 90-91 0.29925471948335414 1104 92-93 0.5091161083637665 1104 94-95 0.626600745892393 1104 96-97 0.827719701970711 1104 98-99 1.0307994727803411 1104 100-101 1.7995273608778994 1105 1 0.10088469964399849 1105 2 0.08067371324671058 1105 3 0.03526655162990977 1105 4 0.05666544374280846 1105 5 -0.12396690988307313 1105 6 -0.0014675961353347589 1105 7 0.012269746110426638 1105 8 0.019291332731697253 1105 9 0.09038793905133957 1105 10-11 0.034514650849438056 1105 12-13 0.03267951369707589 1105 14-15 0.11691302445394314 1105 16-17 0.03755867078778152 1105 18-19 0.07475015651478856 1105 20-21 -0.03501627376294181 1105 22-23 -0.01530253837544393 1105 24-25 0.008856213654624412 1105 26-27 0.02623233474984943 1105 28-29 0.08539841397065828 1105 30-31 0.002463069678370289 1105 32-33 0.042379231437919884 1105 34-35 6.221422079448757E-4 1105 36-37 0.05657731600519611 1105 38-39 -0.13644465898843805 1105 40-41 -0.04226431516557483 1105 42-43 0.011267651752298491 1105 44-45 0.07276480639345806 1105 46-47 0.23895840851408678 1105 48-49 0.2123512937858152 1105 50-51 0.27723438050003324 1105 52-53 0.2421761894569343 1105 54-55 0.2196150603263689 1105 56-57 0.31108548928656177 1105 58-59 0.2637266395881426 1105 60-61 0.34424842402440703 1105 62-63 0.30474639592316066 1105 64-65 0.2819044241622706 1105 66-67 0.2838694962925814 1105 68-69 0.30209802749197934 1105 70-71 0.5110319615428836 1105 72-73 0.6458863241781287 1105 74-75 0.6919084341658461 1105 76-77 0.4673926979924765 1105 78-79 0.6136305485107805 1105 80-81 0.5878064855622753 1105 82-83 0.6212111402911056 1105 84-85 0.7336806839333185 1105 86-87 0.8980243657445435 1105 88-89 0.8517599410368781 1105 90-91 0.7398188934339665 1105 92-93 0.9449766161066186 1105 94-95 1.1560249568565197 1105 96-97 1.0721755340170631 1105 98-99 1.512438207356702 1105 100-101 2.025117747359584 1106 1 -0.0022214043321966415 1106 2 0.09418732636916616 1106 3 -0.04659523113507191 1106 4 0.09178947414106631 1106 5 -0.05401614857525061 1106 6 -0.07982720320860892 1106 7 0.012715081088323643 1106 8 0.0244873169772859 1106 9 0.07679846541886093 1106 10-11 0.1367162641291415 1106 12-13 -0.10232977384066544 1106 14-15 0.0070432035461749365 1106 16-17 0.15503297035264296 1106 18-19 0.2342054166272689 1106 20-21 0.08606385704883479 1106 22-23 0.158492815152961 1106 24-25 0.14172850755359434 1106 26-27 0.021661815802538342 1106 28-29 -0.014438931267761745 1106 30-31 0.02177709410607065 1106 32-33 0.04715905782484242 1106 34-35 -0.1662909431464925 1106 36-37 -0.0067416396627848485 1106 38-39 0.04835123631299609 1106 40-41 0.12451993017283769 1106 42-43 0.35413227974710537 1106 44-45 0.23130037299468142 1106 46-47 0.3893277601309322 1106 48-49 0.31786573860972567 1106 50-51 0.37156907461325517 1106 52-53 0.5319923613206115 1106 54-55 0.4420002990381988 1106 56-57 0.3781196136167111 1106 58-59 0.513692087736402 1106 60-61 0.5812844156901988 1106 62-63 0.7772509536963099 1106 64-65 0.6116163139304973 1106 66-67 0.4982955240028559 1106 68-69 0.41721466225833126 1106 70-71 0.5530660551602722 1106 72-73 0.6477647164021505 1106 74-75 0.7038286694501394 1106 76-77 0.35006704817196166 1106 78-79 0.47594694764886114 1106 80-81 0.5883184672230755 1106 82-83 0.5967981839302468 1106 84-85 0.7432409509623135 1106 86-87 0.5890343731893175 1106 88-89 0.7024871884121673 1106 90-91 0.9803266551619423 1106 92-93 1.3217750015984038 1106 94-95 1.083661094223622 1106 96-97 1.4205265353757923 1106 98-99 1.608888242614949 1106 100-101 2.3912717054968695 1107 1 0.1092441506019668 1107 2 0.14574663289212708 1107 3 0.10782360052896678 1107 4 0.09070919553005297 1107 5 -0.08936639515616918 1107 6 0.004985649503787215 1107 7 0.025781327465871584 1107 8 0.048790331873824755 1107 9 0.22268447894267496 1107 10-11 0.16664905176450873 1107 12-13 0.13409587057439865 1107 14-15 0.14526375365040423 1107 16-17 0.12829058385612768 1107 18-19 0.16524329348933264 1107 20-21 0.2281573035890787 1107 22-23 0.236776815356329 1107 24-25 0.22853393741784345 1107 26-27 0.054909057655194715 1107 28-29 0.09016364130837218 1107 30-31 0.05397293527745717 1107 32-33 0.136206797923478 1107 34-35 0.1848380415502433 1107 36-37 0.13006287505580616 1107 38-39 0.08042322777163946 1107 40-41 0.19196087785700655 1107 42-43 0.1783488642201263 1107 44-45 0.2828820497448987 1107 46-47 0.2839418228692452 1107 48-49 0.13647268354846886 1107 50-51 0.3862122186470245 1107 52-53 0.21773195153871683 1107 54-55 0.07276261537354856 1107 56-57 0.16046767401632422 1107 58-59 0.2459037914325819 1107 60-61 0.41610530134783374 1107 62-63 0.334002186600884 1107 64-65 0.3404214387461977 1107 66-67 0.5286147064727373 1107 68-69 0.6064277386729913 1107 70-71 0.4988243550230038 1107 72-73 0.4885960753934526 1107 74-75 0.6001497838941887 1107 76-77 0.47832735627099865 1107 78-79 0.5417430735322313 1107 80-81 0.5479343094112892 1107 82-83 0.45409647057420344 1107 84-85 0.6888239495895938 1107 86-87 0.7796634849896087 1107 88-89 0.6468115567074513 1107 90-91 0.5653709380436318 1107 92-93 0.754895546618485 1107 94-95 0.6267567269451639 1107 96-97 0.8196935494588686 1107 98-99 1.2789806723266786 1107 100-101 2.0390315306023687 1108 1 0.1353041213249213 1108 2 0.21355793760950803 1108 3 0.10237844776889915 1108 4 0.10040764195777996 1108 5 0.03225763090984657 1108 6 0.047547296573718256 1108 7 0.057324983318920886 1108 8 0.07660692534787472 1108 9 0.1710692359904158 1108 10-11 0.06645147892605507 1108 12-13 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0.6678674040293693 2316 94-95 0.7882589158132092 2316 96-97 0.5083827301968284 2316 98-99 0.5358862383054124 2316 100-101 -0.13542000160526513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 12.0 5 33.0 6 67.0 7 146.0 8 408.0 9 719.0 10 1266.0 11 2169.0 12 3412.0 13 5103.0 14 6468.0 15 9333.0 16 13094.0 17 19239.0 18 18367.0 19 10663.0 20 8793.0 21 8692.0 22 8950.0 23 9597.0 24 10694.0 25 12566.0 26 15451.0 27 19006.0 28 23744.0 29 29911.0 30 37615.0 31 47961.0 32 62835.0 33 83870.0 34 118073.0 35 174979.0 36 281332.0 37 445527.0 38 363930.0 39 32831.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07578265761586 19.825488110476122 15.011882630785822 22.086846601122197 2 15.513049967061733 20.750791127919726 40.37442040042123 23.361738504597312 3 19.553492168226132 24.78847188325609 29.97835033242972 25.67968561608806 4 12.944805272237284 17.082197189518062 36.72562689964571 33.247370638598944 5 13.367927553554344 37.9863089574825 34.077179740649356 14.568583748313795 6 32.00001059959244 36.621485903867 16.965707668540144 14.412795828000412 7 28.432717762002053 33.51368539879642 20.08447875179199 17.969118087409537 8 26.44131933127171 35.879143446934464 19.101419550418285 18.578117671375534 9 26.56422160567926 16.940268646670535 20.305427256321995 36.190082491328205 10-11 24.9484992302046 27.524332689408094 26.622121879413736 20.905046200973572 12-13 25.67233632650033 24.73529883307562 27.77976968911608 21.81259515130797 14-15 22.626473012112687 25.92898803041023 26.35310422314262 25.091434734334467 16-17 21.572343543390758 29.4921205279657 27.083919623290488 21.85161630535306 18-19 21.43189894348562 27.520410840203198 30.085565210017673 20.96212500629351 20-21 22.141014514836137 26.572153508800984 30.471722237311937 20.81510973905094 22-23 22.00107586148416 26.456785347084576 30.36467994445799 21.17745884697328 24-25 21.645614031368552 26.669389005425952 30.51496191022324 21.170035052982257 26-27 21.38995799312499 26.997632012143093 30.38486692523031 21.227543069501607 28-29 21.31195978839512 27.055093004441073 30.529380541901617 21.103566665262186 30-31 21.39065689415173 26.85050505960575 30.423961758618102 21.334876287624414 32-33 21.377549533220446 26.85223903140924 30.323446563479635 21.446764871890675 34-35 21.73666372527976 26.984601442074553 30.20528760669152 21.073447225954162 36-37 21.83416504846136 26.602155746254102 30.142792485308956 21.42088671997558 38-39 21.475463268437327 27.05593669923391 30.2078580078596 21.26074202446916 40-41 21.730966444340215 26.936452793390092 30.17038844856415 21.16219231370554 42-43 21.65657069999144 26.950360812543117 29.89325757979312 21.499810907672327 44-45 21.599584495976128 27.286371839002786 29.710922615025453 21.403121049995626 46-47 21.88373307046344 27.559072853648775 29.04926955558559 21.507924520302193 48-49 21.90980806787979 27.68719542733582 28.874588272080935 21.528408232703452 50-51 22.161124404766635 27.86627554170542 28.582993483900545 21.389606569627396 52-53 22.260248290350624 27.908192617007003 28.405446392916073 21.42611269972629 54-55 22.00618486219205 28.056935710821918 28.474771645030252 21.462107781955783 56-57 21.985038675262935 28.366814795971095 28.399832526439354 21.24831400232661 58-59 22.003057982420575 27.888826174633586 28.492260972565603 21.615854870380232 60-61 21.93418713050483 28.00952903360866 28.754998370312663 21.301285465573848 62-63 21.885667496084775 27.987163893548296 28.912375819149755 21.214792791217178 64-65 21.852649765616512 27.844705371078483 28.731255283234358 21.571389580070647 66-67 21.693417388101427 28.170139358141675 28.506172937650547 21.630270316106344 68-69 21.82116897605287 28.007117626327265 28.627750263002387 21.543963134617474 70-71 21.850635843051837 28.116452422404357 28.433247741624335 21.59966399291947 72-73 21.97169162344676 28.090793073367315 28.178902325660278 21.758612977525647 74-75 21.858814906324703 28.475562752684546 27.959918669262507 21.70570367172824 76-77 21.936572038805107 28.161580187241803 28.303296738240412 21.598551035712678 78-79 22.049828684087096 28.161977671958518 28.10762826169334 21.68056538226105 80-81 21.933895641712574 28.44358234425886 28.003619760820197 21.618902253208365 82-83 21.942465774478702 28.49467594169041 27.93612878966346 21.626729494167424 84-85 21.861299158734393 28.445387693148323 27.865906285020163 21.82740686309712 86-87 21.881772145860992 28.35650669231768 27.972721948841066 21.78899921298026 88-89 21.909755069917562 28.382025211130628 27.81566248777734 21.89255723117446 90-91 21.79949117506758 28.48439073514602 27.88739598240314 21.82872210738326 92-93 21.866554024958017 28.14127665844202 27.95500974264214 22.037159573957823 94-95 21.678789950526404 28.254671107895902 28.004255736366936 22.062283205210758 96-97 21.773417812085125 28.236625301757144 27.891052089047175 22.098904797110553 98-99 21.573731377455502 28.26662504547901 27.71430517216571 22.445338404899783 100-101 21.354394423427877 28.55183936029072 28.002599375492615 22.09116684078878 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 519.0 1 379.5 2 295.5 3 408.5 4 387.5 5 344.5 6 625.5 7 1391.0 8 2363.5 9 2961.0 10 2940.0 11 2823.5 12 3064.5 13 3393.5 14 3644.0 15 3782.5 16 3876.5 17 3946.0 18 4056.0 19 4263.0 20 4516.5 21 4987.5 22 5899.5 23 7273.0 24 8572.0 25 10263.0 26 12824.5 27 15611.5 28 17846.0 29 20279.0 30 23735.0 31 27526.5 32 31516.5 33 35347.5 34 39732.5 35 43336.0 36 47939.0 37 53414.5 38 56357.5 39 57990.5 40 58860.5 41 61530.0 42 63781.0 43 66223.5 44 70032.5 45 71928.0 46 71710.5 47 72112.0 48 72718.5 49 70893.5 50 69379.5 51 66349.0 52 61943.5 53 60008.5 54 58675.5 55 53567.0 56 46501.0 57 41255.5 58 37370.5 59 32941.5 60 26628.5 61 20959.0 62 16566.5 63 12051.0 64 8603.0 65 6500.0 66 4805.5 67 3705.5 68 2791.0 69 1920.0 70 1395.0 71 892.5 72 648.0 73 509.5 74 345.0 75 235.0 76 148.5 77 77.0 78 42.5 79 30.5 80 21.5 81 13.5 82 8.5 83 5.5 84 2.0 85 1.0 86 1.5 87 1.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04870512728785578 2 1.0599592445670465E-4 3 0.0 4 0.0 5 0.012189531312521033 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0035773624504137815 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.324949055708808E-4 22-23 2.649898111417616E-4 24-25 3.7098573559846625E-4 26-27 0.0016429368290789218 28-29 0.002464405243618383 30-31 4.504826789409947E-4 32-33 0.0 34-35 0.0 36-37 5.299796222835232E-5 38-39 0.0 40-41 0.0 42-43 0.011686050671351686 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 7.949694334252848E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.5899388668505696E-4 74-75 7.949694334252848E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.9148879225593773E-4 84-85 2.9148879225593773E-4 86-87 0.0 88-89 0.0 90-91 0.008744663767678133 92-93 0.0029943848659019063 94-95 0.0 96-97 0.0 98-99 2.9148879225593773E-4 100-101 0.0136734742549149 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1886865.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.89594085226121 #Duplication Level Percentage of deduplicated Percentage of total 1 75.6843921154018 40.03397128775638 2 13.562616506002318 14.348147210068005 3 4.427056587779959 7.025199702504661 4 2.0022942339218193 4.236529494654089 5 1.0860867493630404 2.8724790227366013 6 0.6798890133005314 2.157802142018829 7 0.45263915510534697 1.6759941783108947 8 0.3231169000751247 1.3673257947791835 9 0.24022205529715218 1.1436094465566105 >10 1.3742120747842022 13.653893578577234 >50 0.11292790922346721 4.141885038447259 >100 0.051522442150376886 4.904086015736961 >500 0.002419406075982395 0.8523252262138228 >1k 3.024257594977994E-4 0.26528516018604653 >5k 2.0161717299853294E-4 0.7744193731421013 >10k+ 1.0080858649926647E-4 0.5470473283112868 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10259 0.5437060945006664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8672 0.4595983284442713 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5851 0.3100910769980894 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2390 0.12666512972576205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.649898111417616E-5 14-15 0.0 0.0 0.0 0.0 5.299796222835232E-5 16-17 0.0 0.0 0.0 0.0 5.299796222835232E-5 18-19 0.0 0.0 0.0 0.0 5.299796222835232E-5 20-21 0.0 0.0 0.0 0.0 5.299796222835232E-5 22-23 0.0 0.0 0.0 1.0599592445670465E-4 5.299796222835232E-5 24-25 0.0 0.0 0.0 2.649898111417616E-4 5.299796222835232E-5 26-27 0.0 0.0 0.0 4.504826789409947E-4 5.299796222835232E-5 28-29 5.299796222835232E-5 0.0 0.0 0.0012189531312521034 5.299796222835232E-5 30-31 5.299796222835232E-5 0.0 0.0 0.0034978655070712532 5.299796222835232E-5 32-33 5.299796222835232E-5 0.0 0.0 0.006041767694032164 5.299796222835232E-5 34-35 5.299796222835232E-5 0.0 0.0 0.008294181088737137 1.0599592445670465E-4 36-37 5.299796222835232E-5 0.0 0.0 0.012110034369178506 1.0599592445670465E-4 38-39 5.299796222835232E-5 0.0 0.0 0.01793981021429726 1.0599592445670465E-4 40-41 5.299796222835232E-5 0.0 0.0 0.024220068738357012 1.0599592445670465E-4 42-43 5.299796222835232E-5 0.0 0.0 0.029281374131164654 1.0599592445670465E-4 44-45 5.299796222835232E-5 0.0 0.0 0.03301773046826349 1.0599592445670465E-4 46-47 5.299796222835232E-5 0.0 0.0 0.03754905623878761 1.324949055708808E-4 48-49 5.299796222835232E-5 0.0 0.0 0.04340533106502055 1.5899388668505696E-4 50-51 5.299796222835232E-5 0.0 0.0 0.0490231150612259 1.5899388668505696E-4 52-53 5.299796222835232E-5 0.0 0.0 0.0633590638439952 1.5899388668505696E-4 54-55 5.299796222835232E-5 0.0 0.0 0.07379966240298061 1.5899388668505696E-4 56-57 5.299796222835232E-5 0.0 0.0 0.09849671280139279 1.5899388668505696E-4 58-59 5.299796222835232E-5 2.649898111417616E-5 0.0 0.14206103775309842 1.5899388668505696E-4 60-61 5.299796222835232E-5 5.299796222835232E-5 0.0 0.17847063780397643 1.5899388668505696E-4 62-63 5.299796222835232E-5 5.299796222835232E-5 0.0 0.19900734816746296 1.5899388668505696E-4 64-65 5.299796222835232E-5 5.299796222835232E-5 0.0 0.23573493599171114 1.5899388668505696E-4 66-67 5.299796222835232E-5 5.299796222835232E-5 0.0 0.3074941768489001 1.5899388668505696E-4 68-69 5.299796222835232E-5 5.299796222835232E-5 0.0 0.40797831323385614 1.5899388668505696E-4 70-71 7.949694334252848E-5 5.299796222835232E-5 0.0 0.4979953520787126 1.5899388668505696E-4 72-73 1.324949055708808E-4 5.299796222835232E-5 0.0 0.608469604343713 1.8549286779923313E-4 74-75 1.5899388668505696E-4 5.299796222835232E-5 0.0 0.7500801594178703 2.119918489134093E-4 76-77 1.5899388668505696E-4 5.299796222835232E-5 0.0 0.8502463080294562 2.649898111417616E-4 78-79 1.5899388668505696E-4 5.299796222835232E-5 0.0 0.9802503093756045 2.649898111417616E-4 80-81 2.119918489134093E-4 5.299796222835232E-5 0.0 1.1594364196696638 2.649898111417616E-4 82-83 2.119918489134093E-4 5.299796222835232E-5 0.0 1.3256645281988908 3.179877733701139E-4 84-85 2.119918489134093E-4 5.299796222835232E-5 0.0 1.523055438518389 3.7098573559846625E-4 86-87 2.119918489134093E-4 5.299796222835232E-5 0.0 1.7784261195157045 3.7098573559846625E-4 88-89 2.119918489134093E-4 5.299796222835232E-5 0.0 2.1242113240745892 3.7098573559846625E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4620 0.0 54.309685 1 GTATCAA 8345 0.0 41.741005 1 ATCAACG 10775 0.0 32.004345 3 TCAACGC 10885 0.0 31.680922 4 CAACGCA 11065 0.0 31.165552 5 TATCAAC 11170 0.0 30.957638 2 AACGCAG 11515 0.0 29.946823 6 TGGTATC 2000 0.0 28.26224 2 ACGCAGA 13340 0.0 25.745811 7 CGCAGAG 13315 0.0 25.651447 8 GTACATG 8750 0.0 24.819523 1 TACATGG 8680 0.0 24.351736 2 GCAGAGT 14275 0.0 23.626879 9 ACATGGG 8570 0.0 23.61122 3 GAGTACT 8960 0.0 22.426243 12-13 GTACCTG 1375 0.0 22.118853 1 TAAGGTG 925 0.0 22.080877 5 ACTACCG 110 0.0028781972 21.59071 5 GTGGTAT 2735 0.0 21.371405 1 AGTACTT 9210 0.0 21.146982 12-13 >>END_MODULE