FastQCFastQC Report
Thu 26 May 2016
SRR937771_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937771_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791763
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88941.123315941765402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69200.8739989112903735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45160.5703726999114634No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16990.21458441478068563No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15160.19147143779135928No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9950.12566891860316787No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9890.12491111607892766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.049.9768451
GTATCAA59100.041.589581
TATCAAC74500.032.8565832
ATCAACG75050.032.61583
TCAACGC76400.032.1077844
CAACGCA77700.031.570595
AACGCAG79350.030.9485366
CCTATAC1503.3131073E-828.5182383
ACGCAGA90150.026.9791497
CGCAGAG90600.026.8975778
GTACATG58200.026.8753661
TACATGG57600.026.735852
GCGCTTA458.9586125E-426.38405656-57
ACATGGG57300.025.5486263
TGGTATC16200.025.5255852
GTGGTAT17250.025.080361
GCAGAGT95000.024.9517469
GTGTAAG2801.8189894E-1223.7712231
AGTACAT44450.023.6315362
GAGTACT57900.023.5465512-13
Produced by FastQC (version 0.11.5)