##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937771_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 791763 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.858535950783253 33.0 31.0 34.0 30.0 34.0 2 31.961002219098393 33.0 31.0 34.0 30.0 34.0 3 31.960786245378983 34.0 31.0 34.0 30.0 34.0 4 35.56288308496356 37.0 35.0 37.0 33.0 37.0 5 35.57649574430732 37.0 35.0 37.0 33.0 37.0 6 35.662986272407274 37.0 35.0 37.0 33.0 37.0 7 35.61102122731171 37.0 35.0 37.0 33.0 37.0 8 35.60609929991677 37.0 35.0 37.0 33.0 37.0 9 37.241698589098 39.0 37.0 39.0 34.0 39.0 10-11 37.121211776756425 39.0 37.0 39.0 34.0 39.0 12-13 37.00523515243829 39.0 37.0 39.0 33.0 39.0 14-15 38.12716178957592 40.0 38.0 41.0 33.0 41.0 16-17 38.060214357074024 40.0 38.0 41.0 33.0 41.0 18-19 38.06166681191215 40.0 38.0 41.0 33.0 41.0 20-21 38.01071848520327 40.0 38.0 41.0 33.0 41.0 22-23 37.9188980793495 40.0 38.0 41.0 33.0 41.0 24-25 37.92186171872139 40.0 38.0 41.0 33.0 41.0 26-27 37.817752281932854 40.0 38.0 41.0 33.0 41.0 28-29 37.681278362338226 40.0 37.0 41.0 33.0 41.0 30-31 37.371335235417675 39.5 37.0 41.0 32.5 41.0 32-33 37.146843184134646 39.0 36.0 41.0 32.0 41.0 34-35 37.10561178029285 40.0 36.0 41.0 32.0 41.0 36-37 36.95301371748869 39.5 35.5 41.0 31.0 41.0 38-39 36.78097423092516 39.0 35.0 41.0 31.0 41.0 40-41 36.5106338639214 39.0 35.0 41.0 30.5 41.0 42-43 36.16687948287556 39.0 35.0 41.0 30.0 41.0 44-45 35.81180795263229 39.0 35.0 41.0 28.5 41.0 46-47 35.28847079239621 39.0 35.0 40.5 26.0 41.0 48-49 34.81587545768115 39.0 35.0 40.0 23.5 41.0 50-51 33.85514667899359 38.0 33.5 39.5 22.0 40.5 52-53 33.91342674512449 38.0 33.5 39.5 22.0 40.5 54-55 34.19332805397575 38.0 34.0 40.0 21.5 41.0 56-57 33.967908957604735 38.0 34.0 40.0 19.5 41.0 58-59 33.72533384358703 38.0 34.0 40.0 18.5 41.0 60-61 33.399604427082345 37.5 33.0 40.0 16.5 41.0 62-63 33.022006585303934 37.0 33.0 40.0 12.0 41.0 64-65 32.6314654764115 36.5 32.5 39.5 8.0 41.0 66-67 32.19033599700921 36.0 32.0 39.0 7.0 41.0 68-69 31.69688151631233 35.0 31.0 39.0 2.0 40.0 70-71 31.24361772399064 35.0 31.0 38.0 2.0 40.0 72-73 30.804184459238435 35.0 30.5 37.5 2.0 40.0 74-75 30.291915636370984 35.0 30.0 37.0 2.0 39.0 76-77 29.84107681212686 34.5 29.5 36.0 2.0 39.0 78-79 29.315261006134413 34.0 29.0 36.0 2.0 38.5 80-81 28.788189142458034 34.0 29.0 35.0 2.0 37.0 82-83 28.309038815908295 34.0 27.5 35.0 2.0 37.0 84-85 27.83727011744676 34.0 26.5 35.0 2.0 36.0 86-87 27.3486434703314 33.0 26.0 35.0 2.0 36.0 88-89 26.931753567671134 33.0 25.5 35.0 2.0 35.5 90-91 26.43336137202673 33.0 24.5 35.0 2.0 35.0 92-93 25.861611997529565 32.5 23.5 35.0 2.0 35.0 94-95 25.177222855829335 32.0 19.0 34.5 2.0 35.0 96-97 24.41798303280148 32.0 11.5 34.0 2.0 35.0 98-99 23.46370694260783 31.0 2.0 34.0 2.0 35.0 100-101 20.789825364408287 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.10333840983344444 1101 2 0.067185754029083 1101 3 -0.012764205272528528 1101 4 -0.1116371922218562 1101 5 -0.14480636848766437 1101 6 -0.04446314894013881 1101 7 0.056609486776139306 1101 8 0.15067333411302286 1101 9 0.07958921145757358 1101 10-11 0.210455850716194 1101 12-13 0.11190173593841024 1101 14-15 0.15015971875763512 1101 16-17 0.041880060314120726 1101 18-19 0.041036252205138624 1101 20-21 0.029590104784752214 1101 22-23 -0.05516080232990106 1101 24-25 0.027961458681893703 1101 26-27 0.05938435426533317 1101 28-29 0.1469980120055041 1101 30-31 -0.03224074940968791 1101 32-33 -0.12933594904959023 1101 34-35 -0.08504889579055686 1101 36-37 0.12518067581942915 1101 38-39 0.30848783564157145 1101 40-41 0.4915552816282158 1101 42-43 0.5931371027084253 1101 44-45 0.6966176759875466 1101 46-47 0.6868033386059835 1101 48-49 0.6767004924329569 1101 50-51 0.6924817681669708 1101 52-53 0.7364355847039761 1101 54-55 0.7785873939474541 1101 56-57 0.5411602819459276 1101 58-59 0.6018960747603543 1101 60-61 0.8081764832811231 1101 62-63 0.8188796322796748 1101 64-65 1.03401265814162 1101 66-67 1.030606099219625 1101 68-69 0.9469941622869662 1101 70-71 1.116415929537343 1101 72-73 1.1345950991898732 1101 74-75 1.0116563053783487 1101 76-77 0.9468321955141761 1101 78-79 1.0436538163502078 1101 80-81 1.0476565620196716 1101 82-83 0.7804651547777866 1101 84-85 0.8896889834602284 1101 86-87 1.0604712667157692 1101 88-89 1.12036299007163 1101 90-91 1.0016029626300238 1101 92-93 1.0190229866498903 1101 94-95 1.3001401960255379 1101 96-97 1.4865985752947886 1101 98-99 1.6049903078857284 1101 100-101 1.5910928695079107 1102 1 0.2736246094168102 1102 2 0.4231887935427636 1102 3 0.458803677199608 1102 4 0.22650870443467852 1102 5 0.2846726926403349 1102 6 0.26100797567992373 1102 7 0.23119155364543786 1102 8 0.1918967383683423 1102 9 0.31337847184257583 1102 10-11 0.21756915703162605 1102 12-13 0.42068677477664806 1102 14-15 0.5698236836343682 1102 16-17 0.5935095773691685 1102 18-19 0.4781352052615304 1102 20-21 0.5559642182599305 1102 22-23 0.5591903459308227 1102 24-25 0.3857375478409679 1102 26-27 0.4372022367374626 1102 28-29 0.6061862085607217 1102 30-31 0.6347108716642822 1102 32-33 0.7066252971510139 1102 34-35 0.892534690835582 1102 36-37 0.9546829554546932 1102 38-39 0.8588403347972644 1102 40-41 0.7946728939213656 1102 42-43 0.7462103887604314 1102 44-45 0.968991873893664 1102 46-47 1.017255466265567 1102 48-49 1.1232451563978358 1102 50-51 1.1476806030200635 1102 52-53 1.0866445512693303 1102 54-55 1.0256639728059511 1102 56-57 0.9528877388861474 1102 58-59 0.8699326333554254 1102 60-61 0.9529333221868939 1102 62-63 1.0969529183316808 1102 64-65 0.9991426817822813 1102 66-67 1.1032589192128697 1102 68-69 1.1098192213886229 1102 70-71 1.1726997019115437 1102 72-73 1.144093579433033 1102 74-75 1.139421418515802 1102 76-77 1.1212665082463573 1102 78-79 1.3085558764650322 1102 80-81 1.233172435035545 1102 82-83 1.4171799470776847 1102 84-85 1.4486112225686334 1102 86-87 1.3522434720518035 1102 88-89 1.4891467624458272 1102 90-91 1.5909625539842942 1102 92-93 1.6841171271429687 1102 94-95 1.929097896126855 1102 96-97 2.2655267245686836 1102 98-99 2.038247653377116 1102 100-101 2.193940302807473 1103 1 0.2793008489847999 1103 2 0.2920523183341217 1103 3 0.3747769221677011 1103 4 0.18878165485195808 1103 5 0.2266932293773607 1103 6 0.14686953679921544 1103 7 0.24150696847116393 1103 8 0.1707811271754096 1103 9 0.17560233045819018 1103 10-11 0.1580064831648329 1103 12-13 0.21686784170513107 1103 14-15 0.16771682714607294 1103 16-17 0.26516315288872505 1103 18-19 0.2843780460404659 1103 20-21 0.10303405134780519 1103 22-23 0.23531434363987813 1103 24-25 0.19041354194995108 1103 26-27 0.3580447747836146 1103 28-29 0.23037312559556966 1103 30-31 0.234701666951473 1103 32-33 0.1953802063311798 1103 34-35 0.4286294380127984 1103 36-37 0.36217865992204423 1103 38-39 0.247351830460822 1103 40-41 0.4908815749882862 1103 42-43 0.5185880683546102 1103 44-45 0.49097681907448276 1103 46-47 0.4019418923823963 1103 48-49 0.31646537403815245 1103 50-51 0.1838275102365543 1103 52-53 0.23961767350790808 1103 54-55 0.33644678249339677 1103 56-57 0.3519427217040203 1103 58-59 0.48498029331287995 1103 60-61 0.6548601754024048 1103 62-63 0.6496079666052879 1103 64-65 0.7330078429670337 1103 66-67 0.6707396915905477 1103 68-69 0.6551350453126936 1103 70-71 0.7251307688400281 1103 72-73 0.6683081106063256 1103 74-75 0.6249659284159819 1103 76-77 0.8565962415142359 1103 78-79 0.7736802423625981 1103 80-81 0.7334690313372967 1103 82-83 0.992259721255401 1103 84-85 0.9410296074483604 1103 86-87 1.1433230825901752 1103 88-89 1.0782923605476107 1103 90-91 1.161801001046971 1103 92-93 1.009545657661704 1103 94-95 1.021553099857833 1103 96-97 1.1864163089247626 1103 98-99 1.4722069071817288 1103 100-101 1.274603246679181 1104 1 0.1695347476656579 1104 2 0.12998602856580277 1104 3 0.22310859271420114 1104 4 0.15700025589396915 1104 5 0.11826844276833981 1104 6 0.07974837506548482 1104 7 0.2007576058727807 1104 8 -0.03327607269156374 1104 9 0.1142233259132368 1104 10-11 0.06280085186009643 1104 12-13 0.14157131138698986 1104 14-15 0.3345830282324229 1104 16-17 0.3615715333343559 1104 18-19 0.2955187178063383 1104 20-21 0.14462589260708825 1104 22-23 0.2276267258591389 1104 24-25 0.18216611926953874 1104 26-27 0.12367497099246094 1104 28-29 0.05992084757762228 1104 30-31 0.15294243848455835 1104 32-33 0.2865120099006333 1104 34-35 0.19510765550531772 1104 36-37 0.27316413825038666 1104 38-39 0.4242557213820035 1104 40-41 0.21247563329668395 1104 42-43 0.2655396258316003 1104 44-45 0.18448086518910856 1104 46-47 -0.047731092874222725 1104 48-49 0.27904712772400586 1104 50-51 0.3856510331275871 1104 52-53 0.3392463177823828 1104 54-55 0.2752063435176737 1104 56-57 0.16337160985388977 1104 58-59 0.28669850637129457 1104 60-61 0.44344791512084214 1104 62-63 0.5073343827454053 1104 64-65 0.5773044841376347 1104 66-67 0.6260827799399564 1104 68-69 0.6568366305028057 1104 70-71 0.4976837008874746 1104 72-73 0.3404453459460903 1104 74-75 0.34276883275031267 1104 76-77 0.2091569332335581 1104 78-79 0.2018866496345204 1104 80-81 0.42707284466176176 1104 82-83 0.1873988410869032 1104 84-85 0.3121192870847658 1104 86-87 0.6385785974998335 1104 88-89 0.5272836032036352 1104 90-91 0.9142120419515258 1104 92-93 1.0567521757860803 1104 94-95 1.2454861449747838 1104 96-97 1.1941205545738036 1104 98-99 0.8786086364800347 1104 100-101 1.1440523099759297 1105 1 0.15225909580740904 1105 2 0.11248005137301931 1105 3 0.03360341665301547 1105 4 0.02196576968219688 1105 5 0.11290711989978774 1105 6 0.12638299646821594 1105 7 0.07461067333056093 1105 8 -0.10758979081842313 1105 9 0.18744299870105152 1105 10-11 0.1366276321873059 1105 12-13 0.28382474436106264 1105 14-15 0.3088867322142974 1105 16-17 0.18673582790042786 1105 18-19 0.24197558813568065 1105 20-21 0.22292711808808008 1105 22-23 0.3332452870768563 1105 24-25 0.35457589808211054 1105 26-27 0.26332722980804135 1105 28-29 0.28141245447235264 1105 30-31 0.2604436589584722 1105 32-33 0.24557042991889944 1105 34-35 0.3852518591930263 1105 36-37 0.4088326450161972 1105 38-39 0.5966035376725145 1105 40-41 0.619669544696535 1105 42-43 0.6594585362926253 1105 44-45 0.6004477934753112 1105 46-47 0.6938294864440664 1105 48-49 0.8029558988632317 1105 50-51 0.7538723958023681 1105 52-53 0.704872765139271 1105 54-55 0.7359127162691834 1105 56-57 0.7933514563871213 1105 58-59 0.6033845754438616 1105 60-61 0.7594705470884975 1105 62-63 0.7626055783091346 1105 64-65 0.7307011569379611 1105 66-67 0.7212509549872124 1105 68-69 0.6259486261967275 1105 70-71 0.6181645050233158 1105 72-73 0.5824008581070181 1105 74-75 0.9501636529338207 1105 76-77 0.8599530577594479 1105 78-79 0.711056477015692 1105 80-81 0.6470523156645527 1105 82-83 0.8462676182346236 1105 84-85 0.7741957200463254 1105 86-87 0.6098023260163608 1105 88-89 0.6871650607624353 1105 90-91 0.7414980834851939 1105 92-93 0.6673346234934705 1105 94-95 0.885918881214721 1105 96-97 1.02623904694428 1105 98-99 0.8441612295177023 1105 100-101 0.8994643294534406 1106 1 0.2728554815409403 1106 2 0.43088007230145564 1106 3 0.30949254825364747 1106 4 0.4222015883298056 1106 5 0.3294716047145272 1106 6 0.0691941715065667 1106 7 0.2733598254485443 1106 8 0.2545973804229149 1106 9 0.2832821636563807 1106 10-11 0.127335966377089 1106 12-13 0.1542898602055729 1106 14-15 0.30633607885462766 1106 16-17 0.11239222406080529 1106 18-19 0.3331824676121684 1106 20-21 0.06949083677250911 1106 22-23 0.33417585511577386 1106 24-25 0.41603449808173565 1106 26-27 0.27570210297609776 1106 28-29 0.4504712540395843 1106 30-31 0.4298051734299335 1106 32-33 0.5018868006288102 1106 34-35 0.7637893724835223 1106 36-37 0.580401922816911 1106 38-39 0.3786983248097542 1106 40-41 0.5832017417900985 1106 42-43 0.5559571339004421 1106 44-45 0.5241907324633104 1106 46-47 0.8962850275282648 1106 48-49 0.9596940149674751 1106 50-51 0.6987941664229567 1106 52-53 0.8618752896320885 1106 54-55 0.6997934092247107 1106 56-57 0.49829972390487853 1106 58-59 0.45293713665486734 1106 60-61 0.17571288344959868 1106 62-63 0.3638291444775703 1106 64-65 0.46619279770879984 1106 66-67 0.5749238023946077 1106 68-69 0.6946462233504569 1106 70-71 0.5055704438412683 1106 72-73 0.8414584804496172 1106 74-75 0.6810323512784215 1106 76-77 0.7319317481250849 1106 78-79 0.6738804707222137 1106 80-81 0.5040503554921152 1106 82-83 0.6782487004217224 1106 84-85 0.9151207196258859 1106 86-87 0.9464895037978351 1106 88-89 0.8021873813150968 1106 90-91 0.6966953433547225 1106 92-93 0.5700799860693095 1106 94-95 1.0767871684650032 1106 96-97 0.8382316023999685 1106 98-99 0.7309309064536293 1106 100-101 0.7195834720601901 1107 1 0.30426050543109895 1107 2 0.206277255318156 1107 3 0.2554640295682411 1107 4 0.3453253125785807 1107 5 0.2401633084640622 1107 6 0.13191908268417052 1107 7 0.18970584552001668 1107 8 0.2844512569416153 1107 9 0.2614934228682628 1107 10-11 0.3301240862909509 1107 12-13 0.41057491416349023 1107 14-15 0.30829110596337017 1107 16-17 0.32900465940371504 1107 18-19 0.3572138311060158 1107 20-21 0.1923626812673902 1107 22-23 0.3863832811040382 1107 24-25 0.481907387057376 1107 26-27 0.39063236363945464 1107 28-29 0.4363219659684674 1107 30-31 0.38872468611632627 1107 32-33 0.4138555534191113 1107 34-35 0.591904801104782 1107 36-37 0.486876249978188 1107 38-39 0.4430200664139292 1107 40-41 0.4678624101331934 1107 42-43 0.6585135161347111 1107 44-45 0.4991875657969942 1107 46-47 0.4840380234022632 1107 48-49 0.588913727621069 1107 50-51 0.4960392705491756 1107 52-53 0.3118513060281529 1107 54-55 0.39345322412527395 1107 56-57 0.17480451335790548 1107 58-59 0.03904865729841589 1107 60-61 0.23851670172241057 1107 62-63 0.49082907595298764 1107 64-65 0.6085443122059004 1107 66-67 0.6515581656039728 1107 68-69 0.8392310162920147 1107 70-71 0.7502738050758744 1107 72-73 0.5627700409620182 1107 74-75 0.6862101102753861 1107 76-77 0.6472218666726093 1107 78-79 0.845258375489653 1107 80-81 0.8164300481282183 1107 82-83 0.494133529286767 1107 84-85 0.6262605326160688 1107 86-87 0.570126539465921 1107 88-89 0.7073410528830024 1107 90-91 0.6779144164455353 1107 92-93 0.5599404243359309 1107 94-95 0.6220632290905712 1107 96-97 0.48714382656256205 1107 98-99 0.5362224577878862 1107 100-101 1.1468788898421138 1108 1 -0.0482212706917835 1108 2 0.015409426017630068 1108 3 0.04644308581359269 1108 4 0.1876760681444125 1108 5 0.09129062743622995 1108 6 0.17219001398060385 1108 7 0.16242733763545658 1108 8 0.05301549249556814 1108 9 0.3015470828925473 1108 10-11 0.30503124767168543 1108 12-13 0.35940354294256593 1108 14-15 0.40460307561715325 1108 16-17 0.19751268349092044 1108 18-19 0.4079845461278282 1108 20-21 0.3904921550124456 1108 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-0.3225684510624838 2316 82-83 -0.22786845689928725 2316 84-85 -0.21192318350148653 2316 86-87 -0.48129699541723525 2316 88-89 -0.785812900906695 2316 90-91 -0.744196830282231 2316 92-93 -0.7901055747583037 2316 94-95 -0.5836735586088864 2316 96-97 -0.40036046363993094 2316 98-99 -0.5779873021435549 2316 100-101 -0.43612358172069676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3003.0 3 543.0 4 508.0 5 560.0 6 651.0 7 798.0 8 884.0 9 1110.0 10 1568.0 11 2056.0 12 3167.0 13 4433.0 14 4975.0 15 6858.0 16 9383.0 17 13022.0 18 10945.0 19 6336.0 20 5985.0 21 6100.0 22 6515.0 23 6856.0 24 7573.0 25 8572.0 26 9999.0 27 11243.0 28 13464.0 29 16040.0 30 19697.0 31 24330.0 32 30982.0 33 40337.0 34 55468.0 35 79206.0 36 111267.0 37 147030.0 38 108341.0 39 11955.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.223307450800604 19.047914558513057 13.973148191536652 20.755629799149688 2 15.866583107025972 21.16983496970963 40.65151198637725 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33.20940372079841 20.10038361876999 22-23 21.11920768178221 25.441823303573745 33.202617190638286 20.23635182400576 24-25 20.687933983845173 25.52312134340142 33.44218778468328 20.346756888070125 26-27 20.294258492763415 25.858702026269192 33.281903254518156 20.56513622644923 28-29 20.26952135002347 26.048964542524804 33.385105152677305 20.296408954774417 30-31 20.36931591714022 25.691984932285123 33.409949905035944 20.528749245538712 32-33 20.133885950832564 25.908468877938045 33.19431430742396 20.763330863805432 34-35 20.644926995246056 25.969990800856902 33.059531235962446 20.325550967934596 36-37 20.644420167971546 25.71943299324483 33.034488124524394 20.601658714259237 38-39 20.408506643878376 26.09630160494241 32.929119524026696 20.566072227152514 40-41 20.458072053961082 26.118785989500537 32.918322692386276 20.5048192641521 42-43 20.37121971740994 26.182883278980977 32.569785574432146 20.876111429176934 44-45 20.445261528358834 26.480288026297394 32.35690259121181 20.717547854131965 46-47 20.809873144167238 26.80275474285946 31.510566258322303 20.876805854651 48-49 20.767666722337783 26.97796942137467 31.16184352367794 21.09252033260961 50-51 21.00959527998517 27.367836358310672 30.746017677280502 20.87655068442366 52-53 21.051142907041196 27.49190688680272 30.46016214068223 20.996788065473858 54-55 20.747726336791246 27.478857478307127 30.595469816595067 21.177946368306554 56-57 20.91251457963835 27.81617890765934 30.225899003903926 21.045407508798387 58-59 20.97867552039814 27.2402784902524 30.496289263501193 21.28475672584827 60-61 20.724037079766024 27.45606644807416 30.870752120144918 20.949144352014898 62-63 20.816913382360198 27.39474047094633 30.975987126336733 20.812359020356734 64-65 20.628390413731683 27.465893449923083 30.88584679378188 21.019869342563354 66-67 20.58861852383633 27.631863579149773 30.553197679934723 21.226320217079174 68-69 20.779411373996258 27.502191880994463 30.691837346615774 21.02655939839351 70-71 20.73243474207091 27.741016459413796 30.31401355793687 21.212535240578422 72-73 20.735853436535994 27.84548950104588 30.035668043672004 21.38298901874612 74-75 20.525104652795612 28.229435361955087 29.954862354216665 21.290597631032636 76-77 20.723901645420774 27.925724872591058 29.986216999708397 21.364156482279768 78-79 20.780920475836844 27.92062385585731 29.852524989278113 21.44593067902773 80-81 20.760689085889048 28.08445635019783 29.698627710816698 21.456226853096428 82-83 20.85189554348823 27.913811043394933 29.782630009266608 21.45166340385023 84-85 20.72071331301308 27.784710727335632 29.66527241642563 21.82930354322566 86-87 20.78448337472812 27.743424427161305 29.648596530174554 21.823495667936022 88-89 20.74171897255301 27.577535412413255 29.710122928320065 21.97062268671367 90-91 20.57059326949412 27.656897652109397 29.927366502820142 21.845142575576347 92-93 20.562185848202738 27.482111492223805 30.055729034567687 21.89997362500577 94-95 20.413729415522603 27.438970009203125 30.01837462088508 22.128925954389196 96-97 20.457557060224353 27.376004503464568 29.83311248225021 22.333325954060868 98-99 20.476358544055195 27.562153936068068 29.635153580595496 22.326333939281238 100-101 20.76385371033361 26.98620494694283 30.197145366960555 22.052795975763004 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1541.0 1 1005.5 2 550.5 3 690.0 4 617.0 5 559.0 6 1044.5 7 2200.5 8 3613.5 9 4127.5 10 3343.0 11 2581.5 12 2389.0 13 2412.0 14 2558.0 15 2640.0 16 2710.0 17 2721.5 18 2783.5 19 2912.0 20 3037.0 21 3369.5 22 3893.0 23 4530.5 24 5196.0 25 6032.0 26 7093.0 27 8278.5 28 9390.0 29 10441.0 30 11659.5 31 12884.0 32 14373.0 33 15481.0 34 16270.5 35 17256.0 36 18452.5 37 19853.5 38 21004.5 39 21585.0 40 21898.0 41 23063.5 42 24097.0 43 24892.0 44 26346.5 45 27672.0 46 27803.5 47 27800.5 48 28727.5 49 28284.0 50 26664.5 51 25086.0 52 23864.5 53 23436.5 54 22934.5 55 21369.0 56 19210.0 57 17131.5 58 15577.0 59 14128.5 60 11825.0 61 9762.0 62 7815.5 63 5570.5 64 3947.5 65 2938.5 66 2119.0 67 1536.5 68 1113.0 69 779.0 70 560.5 71 384.0 72 263.5 73 215.0 74 175.5 75 138.5 76 92.0 77 62.5 78 43.0 79 27.5 80 24.5 81 20.0 82 12.0 83 6.5 84 5.5 85 4.5 86 3.5 87 5.5 88 5.5 89 3.0 90 1.0 91 0.5 92 1.0 93 2.0 94 1.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.274071912933542 2 0.24098120270838624 3 0.24123380354979965 4 0.24325461028110684 5 0.2504537342613888 6 0.18212520665906337 7 0.18124110371411647 8 0.1840197129696639 9 0.17959919824492937 10-11 0.19083993568782576 12-13 0.18338821086613039 14-15 0.18174630539694328 16-17 0.18698777285627138 18-19 0.18162000497623657 20-21 0.17618908688584842 22-23 0.17347362784065434 24-25 0.17820989361715564 26-27 0.17296842615782754 28-29 0.18136740413482316 30-31 0.1849038159146108 32-33 0.18484066570425745 34-35 0.18591421928026441 36-37 0.16816901017097288 38-39 0.16400109628765175 40-41 0.1694320143780399 42-43 0.17625223709620177 44-45 0.1791571467724559 46-47 0.17896769614139585 48-49 0.17770469193432883 50-51 0.1801675501381095 52-53 0.20018616682012166 54-55 0.17094761942652031 56-57 0.16128563724245765 58-59 0.16248549123917133 60-61 0.1580018263040834 62-63 0.1657061519671922 64-65 0.16406424649800508 66-67 0.16014893345609732 68-69 0.17000036627122006 70-71 0.16463259839118524 72-73 0.1616013882942244 74-75 0.16318014355305815 76-77 0.16374849544623832 78-79 0.16722175701567263 80-81 0.16362219502553163 82-83 0.16299069292199814 84-85 0.15844387777655689 86-87 0.1521288567412218 88-89 0.15421281368288237 90-91 0.16261179165987802 92-93 0.15680197230736975 94-95 0.16046468450786408 96-97 0.15920168030079707 98-99 0.15989633261468394 100-101 0.14568753528518003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 791763.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.313569967482536 #Duplication Level Percentage of deduplicated Percentage of total 1 78.45744035001746 44.96675996970313 2 12.073479093206185 13.839483775188205 3 3.9206959411067874 6.741272434255459 4 1.8081616864898693 4.145288053246333 5 0.9627851944832875 2.759032830383709 6 0.5888132221717938 2.0248192684033164 7 0.3874401524633022 1.5543904800492299 8 0.3017686627297511 1.3836351492284185 9 0.2206488681394751 1.138155690812187 >10 1.179930129046637 12.136593147290881 >50 0.06670415253836313 2.583154609924698 >100 0.029694906066368043 3.1277245086151693 >500 0.0013296226585946414 0.6092623430833445 >1k 6.648113292973207E-4 0.9819068531118065 >5k 4.432075528648805E-4 2.0085208867041917 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8894 1.123315941765402 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6920 0.8739989112903735 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4516 0.5703726999114634 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1699 0.21458441478068563 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1516 0.19147143779135928 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 995 0.12566891860316787 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 989 0.12491111607892766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.2630042070670136E-4 0.0 16-17 0.0 0.0 0.0 1.2630042070670136E-4 0.0 18-19 6.315021035335068E-5 0.0 0.0 1.2630042070670136E-4 0.0 20-21 1.2630042070670136E-4 0.0 0.0 2.526008414134027E-4 0.0 22-23 1.2630042070670136E-4 0.0 0.0 5.052016828268055E-4 0.0 24-25 1.2630042070670136E-4 0.0 0.0 8.841029449469096E-4 0.0 26-27 1.2630042070670136E-4 0.0 0.0 0.0019576565209538715 0.0 28-29 1.2630042070670136E-4 0.0 0.0 0.004546815145441249 0.0 30-31 1.2630042070670136E-4 0.0 0.0 0.009093630290882499 0.0 32-33 1.2630042070670136E-4 0.0 0.0 0.014587698591624008 0.0 34-35 1.2630042070670136E-4 0.0 0.0 0.02033436773377892 0.0 36-37 1.2630042070670136E-4 0.0 0.0 0.02949114823501477 0.0 38-39 1.2630042070670136E-4 0.0 0.0 0.04079503588826454 0.0 40-41 1.2630042070670136E-4 0.0 0.0 0.05614053700412876 0.0 42-43 1.8945063106005206E-4 0.0 0.0 0.07514875032048732 0.0 44-45 2.526008414134027E-4 0.0 0.0 0.0919467062744786 0.0 46-47 2.526008414134027E-4 0.0 0.0 0.12023800051277969 0.0 48-49 2.526008414134027E-4 0.0 0.0 0.1594542811422105 0.0 50-51 2.526008414134027E-4 0.0 0.0 0.17934659740351594 0.0 52-53 2.526008414134027E-4 0.0 0.0 0.2106691017387779 0.0 54-55 2.526008414134027E-4 0.0 0.0 0.26093666918004504 0.0 56-57 2.526008414134027E-4 0.0 0.0 0.3425898911669275 0.0 58-59 2.526008414134027E-4 0.0 0.0 0.45253440739211104 0.0 60-61 3.157510517667534E-4 0.0 0.0 0.5678466914973294 0.0 62-63 3.789012621201041E-4 0.0 0.0 0.6857481342270351 0.0 64-65 3.789012621201041E-4 0.0 0.0 0.8128063574579767 0.0 66-67 3.789012621201041E-4 0.0 0.0 0.9730815913347808 0.0 68-69 4.420514724734548E-4 0.0 0.0 1.1698576467958215 0.0 70-71 5.052016828268055E-4 0.0 0.0 1.29817887423383 0.0 72-73 5.052016828268055E-4 0.0 0.0 1.4728523560711981 0.0 74-75 5.052016828268055E-4 0.0 0.0 1.6609768327138297 0.0 76-77 5.052016828268055E-4 0.0 0.0 1.8681095226728202 0.0 78-79 5.052016828268055E-4 0.0 0.0 2.1025862537148114 0.0 80-81 6.315021035335069E-4 0.0 0.0 2.4197897603196914 0.0 82-83 6.315021035335069E-4 0.0 0.0 2.787071383734779 0.0 84-85 6.315021035335069E-4 0.0 0.0 3.2252075431663263 0.0 86-87 6.315021035335069E-4 0.0 0.0 3.7499857912026706 0.0 88-89 6.315021035335069E-4 0.0 0.0 4.362858582681939 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3320 0.0 49.976845 1 GTATCAA 5910 0.0 41.58958 1 TATCAAC 7450 0.0 32.856583 2 ATCAACG 7505 0.0 32.6158 3 TCAACGC 7640 0.0 32.107784 4 CAACGCA 7770 0.0 31.57059 5 AACGCAG 7935 0.0 30.948536 6 CCTATAC 150 3.3131073E-8 28.518238 3 ACGCAGA 9015 0.0 26.979149 7 CGCAGAG 9060 0.0 26.897577 8 GTACATG 5820 0.0 26.875366 1 TACATGG 5760 0.0 26.73585 2 GCGCTTA 45 8.9586125E-4 26.384056 56-57 ACATGGG 5730 0.0 25.548626 3 TGGTATC 1620 0.0 25.525585 2 GTGGTAT 1725 0.0 25.08036 1 GCAGAGT 9500 0.0 24.951746 9 GTGTAAG 280 1.8189894E-12 23.771223 1 AGTACAT 4445 0.0 23.631536 2 GAGTACT 5790 0.0 23.54655 12-13 >>END_MODULE