FastQCFastQC Report
Thu 26 May 2016
SRR937771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937771_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791763
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84581.0682489583372803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73620.9298236972427355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50080.6325125068991605No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17030.21508961646351243No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.21407921309785882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13810.1744208809959546No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10110.1276897253344751No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8030.1014192378274812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40350.046.9963151
CGTATGA500.001614119938.0066682
GTATCAA64500.035.294761
TAAGGTG3200.028.2080755
TATCAAC81600.027.8878612
ATCAACG82350.027.6338733
TCAACGC83100.027.4416394
CAACGCA84200.027.0831385
AGGTAAG3700.026.9641912
AACGCAG86450.026.3715926
GTAAGGT3300.025.913644
GTACATG59700.024.7582821
GGTAAGG3650.024.7303663
GGTGTGC5050.024.4581938
TACCTGG5650.024.3848112
TACATGG60550.023.8522452
CCTAGAC1601.8555038E-623.754173
GGGCGTA1000.001652111923.7526689
GAGTACT61200.023.09287312-13
ACATGGG59950.022.9022673
Produced by FastQC (version 0.11.5)