##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937771_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 791763 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.142758628528995 33.0 31.0 34.0 31.0 34.0 2 32.421216955073675 34.0 31.0 34.0 31.0 34.0 3 32.488514871243034 34.0 31.0 34.0 31.0 34.0 4 36.12858140630466 37.0 35.0 37.0 35.0 37.0 5 36.060763132401995 37.0 35.0 37.0 35.0 37.0 6 36.118083315335525 37.0 35.0 37.0 35.0 37.0 7 36.05980072319621 37.0 35.0 37.0 35.0 37.0 8 36.046391407529775 37.0 35.0 37.0 35.0 37.0 9 37.72697764356253 39.0 37.0 39.0 35.0 39.0 10-11 37.651041410118935 39.0 37.0 39.0 35.0 39.0 12-13 37.54160335858079 39.0 37.0 39.0 35.0 39.0 14-15 38.71285851953173 40.0 38.0 41.0 34.5 41.0 16-17 38.68148044301135 40.0 38.0 41.0 34.5 41.0 18-19 38.71705674551602 40.0 38.0 41.0 35.0 41.0 20-21 38.65283298158666 40.0 38.0 41.0 34.0 41.0 22-23 38.585429604566016 40.0 38.0 41.0 34.0 41.0 24-25 38.57170769535833 40.0 38.0 41.0 34.0 41.0 26-27 38.41794956318999 40.0 38.0 41.0 34.0 41.0 28-29 38.24826810548106 40.0 38.0 41.0 34.0 41.0 30-31 38.205862739228785 40.0 37.5 41.0 34.0 41.0 32-33 38.04611417810632 40.0 37.0 41.0 34.0 41.0 34-35 37.87216692368802 40.0 37.0 41.0 33.0 41.0 36-37 37.696757741900036 40.0 36.5 41.0 33.0 41.0 38-39 37.529006786121606 40.0 36.0 41.0 33.0 41.0 40-41 37.308034096061576 40.0 36.0 41.0 33.0 41.0 42-43 37.02001230166098 40.0 36.0 41.0 32.0 41.0 44-45 36.606809739783245 39.5 35.0 41.0 31.0 41.0 46-47 36.051504048559984 39.0 35.0 41.0 29.5 41.0 48-49 35.56208170879417 39.0 35.0 41.0 27.5 41.0 50-51 35.23387364653312 39.0 35.0 40.5 26.5 41.0 52-53 34.973215469780726 39.0 35.0 40.0 26.0 41.0 54-55 34.72701300768033 38.0 35.0 40.0 25.0 41.0 56-57 34.43476393819868 38.0 34.0 40.0 24.0 41.0 58-59 34.151606604501595 38.0 34.0 40.0 23.0 41.0 60-61 33.81653600888144 37.5 34.0 40.0 22.0 41.0 62-63 33.435653093160454 37.0 33.0 40.0 21.5 41.0 64-65 33.01139608696037 36.0 33.0 39.0 19.5 41.0 66-67 32.576205884841805 36.0 32.5 39.0 18.0 40.0 68-69 32.12465914673962 35.0 32.0 39.0 14.0 40.0 70-71 31.724821063878963 35.0 31.0 38.0 10.0 40.0 72-73 31.254056453762047 35.0 31.0 37.0 7.5 39.5 74-75 30.795652865819697 35.0 31.0 37.0 6.5 39.0 76-77 29.576388388949724 33.5 29.5 35.5 2.0 38.0 78-79 30.142289422466064 34.0 30.0 36.0 2.0 38.0 80-81 29.957516327486886 34.0 30.0 35.5 2.0 37.0 82-83 29.594316102166935 34.0 30.0 35.0 2.0 37.0 84-85 29.233859121984736 34.0 29.5 35.0 2.0 36.5 86-87 28.87779423893261 34.0 29.0 35.0 2.0 36.0 88-89 28.455865580988252 34.0 29.0 35.0 2.0 36.0 90-91 27.928882127606368 34.0 27.5 35.0 2.0 35.0 92-93 27.50759002378237 33.0 27.0 35.0 2.0 35.0 94-95 27.056415871921267 33.0 26.0 35.0 2.0 35.0 96-97 26.508002394655975 33.0 25.0 35.0 2.0 35.0 98-99 25.6647784753771 32.5 23.5 34.5 2.0 35.0 100-101 23.05830911522766 29.5 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2125474676065835 1101 2 0.10451385499909094 1101 3 0.176814869407508 1101 4 0.06948343693791514 1101 5 0.0026334362165130187 1101 6 0.002610649205827542 1101 7 0.06734119955466156 1101 8 -0.048355753755998876 1101 9 0.11454928692403854 1101 10-11 0.12744028349863612 1101 12-13 0.08411880723706133 1101 14-15 0.005428508628121165 1101 16-17 0.15604924998052638 1101 18-19 0.09430727035771014 1101 20-21 0.08185257382654498 1101 22-23 0.12531395245296295 1101 24-25 0.12011231487534246 1101 26-27 -0.169739403545357 1101 28-29 -0.27464828966556354 1101 30-31 -0.214068005960506 1101 32-33 -0.08117399013546844 1101 34-35 0.07122482745288039 1101 36-37 -0.17227012183919754 1101 38-39 -0.2808125282653151 1101 40-41 -0.21856034179356953 1101 42-43 -0.411317226519472 1101 44-45 -0.5276875436275787 1101 46-47 -0.7540780452364046 1101 48-49 -0.7619173194917082 1101 50-51 -0.6693078672592776 1101 52-53 -0.6206110990494551 1101 54-55 -0.8343208324885083 1101 56-57 -0.8002979803177013 1101 58-59 -0.6995841088349124 1101 60-61 -0.6889300554057414 1101 62-63 -0.8955449935389339 1101 64-65 -0.8506977215461404 1101 66-67 -0.6712348188294968 1101 68-69 -0.8435374838370713 1101 70-71 -0.7964726025840214 1101 72-73 -0.9294049374394611 1101 74-75 -1.0110130402939284 1101 76-77 -0.6274782978493683 1101 78-79 -0.7511817908477703 1101 80-81 -0.7148543311467392 1101 82-83 -0.7470198284887282 1101 84-85 -0.6630869701357511 1101 86-87 -0.7385050776286839 1101 88-89 -0.6537481881130667 1101 90-91 -0.7121132834151744 1101 92-93 -0.9742772871041012 1101 94-95 -0.7871642667707306 1101 96-97 -1.1053915045731948 1101 98-99 -1.353255617182164 1101 100-101 -0.9181412692968145 1102 1 0.3919420977990882 1102 2 0.1946438786397522 1102 3 0.1410372604916006 1102 4 0.13548732076094439 1102 5 0.13058851895883095 1102 6 0.07467245265060996 1102 7 0.10048034173635756 1102 8 0.08276548906736991 1102 9 0.22264199209121216 1102 10-11 0.2383297634040673 1102 12-13 0.26499097880071787 1102 14-15 0.2878922033258604 1102 16-17 0.30393003349961134 1102 18-19 0.2128271960584911 1102 20-21 0.3214147994260088 1102 22-23 0.20299041310814658 1102 24-25 0.23623651278145985 1102 26-27 0.24392996491123853 1102 28-29 0.20437062286399055 1102 30-31 0.24783253439748165 1102 32-33 0.2753778504589235 1102 34-35 0.14737097740221827 1102 36-37 0.12930662588116348 1102 38-39 0.22265969395691343 1102 40-41 0.3674220121409135 1102 42-43 0.34551112538866846 1102 44-45 0.367069295278192 1102 46-47 0.18093124216650125 1102 48-49 0.2606468480192703 1102 50-51 0.11598426377753412 1102 52-53 0.1683115622136242 1102 54-55 -0.09382986659860393 1102 56-57 0.00840262758503485 1102 58-59 0.03268539471118714 1102 60-61 0.04665336235852635 1102 62-63 -0.15835738800026178 1102 64-65 -0.10206972424792582 1102 66-67 0.07910290491248162 1102 68-69 -0.12300388032474174 1102 70-71 -0.1780054462179308 1102 72-73 -0.22119825050936726 1102 74-75 -0.21134654248913165 1102 76-77 -0.30560646400945046 1102 78-79 -0.02847409074645313 1102 80-81 -0.013919258536137846 1102 82-83 -0.007742557296563035 1102 84-85 0.017489693153677877 1102 86-87 0.006386850773882458 1102 88-89 0.05037415231246811 1102 90-91 0.11535209990127271 1102 92-93 0.28013422927550025 1102 94-95 0.0772290969577405 1102 96-97 -0.08835756333712297 1102 98-99 -0.07999489445335684 1102 100-101 -0.2909038495062042 1103 1 0.383479898486371 1103 2 0.1583445822889118 1103 3 0.2475112418367118 1103 4 0.163099413708089 1103 5 0.2107022482993628 1103 6 0.1777775091061784 1103 7 0.18394049555733716 1103 8 0.13294549234015562 1103 9 0.18444243391743242 1103 10-11 0.2019026238197128 1103 12-13 0.26583576688777555 1103 14-15 0.322399648915777 1103 16-17 0.3267863581600494 1103 18-19 0.2863564875001501 1103 20-21 0.17995575017056353 1103 22-23 0.040161088938617695 1103 24-25 0.06618967102070172 1103 26-27 0.06537899125718383 1103 28-29 0.1322752211294116 1103 30-31 0.363171676927351 1103 32-33 0.22487056850834364 1103 34-35 0.2965640309122932 1103 36-37 0.22773875646536368 1103 38-39 0.3781958173409663 1103 40-41 0.3533643701837903 1103 42-43 0.3788884367943268 1103 44-45 0.25228039002536917 1103 46-47 0.1769077599213702 1103 48-49 0.23019561254226062 1103 50-51 0.44805757420135706 1103 52-53 0.3639996656336919 1103 54-55 0.20664366473832985 1103 56-57 0.47803321177747904 1103 58-59 0.31091318067093 1103 60-61 0.5257504823061581 1103 62-63 0.3801571759008979 1103 64-65 0.3770642145509555 1103 66-67 0.5434049831320849 1103 68-69 0.3964692010040096 1103 70-71 0.26810505124565864 1103 72-73 0.4882764672127742 1103 74-75 0.5885655013662081 1103 76-77 0.6617168055045397 1103 78-79 0.5520916922677834 1103 80-81 0.6843246868083241 1103 82-83 0.5672042599182952 1103 84-85 0.8456581598530732 1103 86-87 0.8494689977220098 1103 88-89 0.6079434944823241 1103 90-91 0.6622879532804227 1103 92-93 0.5165869375065597 1103 94-95 0.5306685913121818 1103 96-97 0.3043338946893819 1103 98-99 0.5566316805752791 1103 100-101 0.6617634717178156 1104 1 0.3194958612399432 1104 2 0.14273655657114404 1104 3 0.15795354313368648 1104 4 0.13444405399187076 1104 5 0.14324852919948228 1104 6 0.09552498721281921 1104 7 0.14803538525914206 1104 8 0.11566359454612041 1104 9 0.17074107043222853 1104 10-11 0.2012873926923433 1104 12-13 0.15768811141720107 1104 14-15 0.045867113720120756 1104 16-17 0.18489521527123998 1104 18-19 0.15208090829607102 1104 20-21 -0.0678549138356459 1104 22-23 0.19779328049165912 1104 24-25 0.12249163310915634 1104 26-27 0.22932423014422199 1104 28-29 0.27486473448713156 1104 30-31 0.2657600817605186 1104 32-33 0.1964479989484289 1104 34-35 0.3988974750980816 1104 36-37 0.37023404523601045 1104 38-39 0.43032418448430576 1104 40-41 0.18347743968828212 1104 42-43 0.3834943563154525 1104 44-45 0.30213073921059674 1104 46-47 0.23366421707689256 1104 48-49 0.20841310505972643 1104 50-51 0.3244072028456344 1104 52-53 0.4277841684604269 1104 54-55 0.3143146546507509 1104 56-57 0.14106391790762274 1104 58-59 0.14297725339014278 1104 60-61 0.14726140127301335 1104 62-63 0.11227113222502538 1104 64-65 -0.2007320386360476 1104 66-67 -0.018234524683016673 1104 68-69 0.2723366214673746 1104 70-71 0.018138306982272923 1104 72-73 -0.06820285880429822 1104 74-75 -0.26634526240720646 1104 76-77 -0.145372209017129 1104 78-79 -0.0033012796865463656 1104 80-81 0.06443775631281312 1104 82-83 -0.016472716026722622 1104 84-85 0.04115200754179682 1104 86-87 -0.09175703043451122 1104 88-89 0.018164090868534544 1104 90-91 -0.14584861694460827 1104 92-93 0.14323586778036201 1104 94-95 -0.07884809198235487 1104 96-97 0.043958104865641445 1104 98-99 0.10987965751796835 1104 100-101 0.0435559559213452 1105 1 0.2867082988080085 1105 2 0.18467704751459735 1105 3 0.21363344098941184 1105 4 0.044033849578141826 1105 5 -0.12748888740146214 1105 6 0.0077272228706846136 1105 7 0.06364117844201189 1105 8 0.08780549498048629 1105 9 0.24129948419166425 1105 10-11 0.17090698035992347 1105 12-13 0.15081590442483161 1105 14-15 0.1015482633439646 1105 16-17 0.07313610552873229 1105 18-19 0.27511469351770046 1105 20-21 0.084830754671124 1105 22-23 0.05094022555155675 1105 24-25 -0.0748158136594057 1105 26-27 0.03178082508457436 1105 28-29 0.022045752675325048 1105 30-31 -0.008693834396048317 1105 32-33 0.17312255175426827 1105 34-35 0.036068729494914464 1105 36-37 -0.017945508383483855 1105 38-39 0.04384515785919518 1105 40-41 0.1405899861601725 1105 42-43 -0.07806822583182793 1105 44-45 -0.055605187786284205 1105 46-47 -0.04157393180840785 1105 48-49 -0.05180695577620753 1105 50-51 0.03801892940940377 1105 52-53 0.05714501565709185 1105 54-55 0.11234437162597999 1105 56-57 0.15797092405380653 1105 58-59 0.29061266340679026 1105 60-61 0.04487365357940121 1105 62-63 0.08173237122511523 1105 64-65 -0.04333471491629837 1105 66-67 -0.03714060452078627 1105 68-69 0.24687065078377657 1105 70-71 0.12056235595144216 1105 72-73 0.29906340479303495 1105 74-75 -0.03366785557004803 1105 76-77 -0.15589957892713002 1105 78-79 0.08843579893700948 1105 80-81 -0.1582517926751308 1105 82-83 -0.15987589802129065 1105 84-85 -0.0014218780716319657 1105 86-87 -0.0917639151591878 1105 88-89 -0.04790423063559146 1105 90-91 0.010659951408193535 1105 92-93 0.1284894134135719 1105 94-95 -0.0030015837895298603 1105 96-97 0.041496502852798045 1105 98-99 0.15466103621537997 1105 100-101 -0.1118704588895767 1106 1 0.2065822797145458 1106 2 0.08351047396702427 1106 3 0.07318680570293878 1106 4 -0.031458015425961605 1106 5 0.08241213229929656 1106 6 0.04103146816692771 1106 7 -0.04663487139008993 1106 8 0.01271911845920215 1106 9 0.07828003382476112 1106 10-11 -0.0956110694833967 1106 12-13 -0.42984360297206337 1106 14-15 -0.0842542207868604 1106 16-17 -0.12849818123197565 1106 18-19 0.01921876017477331 1106 20-21 0.18356813454282417 1106 22-23 0.1443137589258754 1106 24-25 0.07582876353944101 1106 26-27 -0.07979484336411957 1106 28-29 -0.022778476476119636 1106 30-31 0.06817986808751897 1106 32-33 0.21362468477317265 1106 34-35 -0.22159304078932252 1106 36-37 0.13219921550128078 1106 38-39 0.31843840806427437 1106 40-41 0.06108060853827624 1106 42-43 -0.14134124664628445 1106 44-45 0.29651017275277525 1106 46-47 0.1814774344261707 1106 48-49 0.3607560864712056 1106 50-51 0.6049712443375483 1106 52-53 0.5681688834192684 1106 54-55 0.45188865489238594 1106 56-57 0.45125308237369666 1106 58-59 0.4766393808000089 1106 60-61 0.591251632378146 1106 62-63 0.4544145934514958 1106 64-65 -0.01312956016731448 1106 66-67 0.16627073084435295 1106 68-69 0.4758903590189334 1106 70-71 0.3776448569745092 1106 72-73 0.35210298009523555 1106 74-75 0.3949157189697594 1106 76-77 0.4970620753163928 1106 78-79 0.4117680173327294 1106 80-81 0.36475360574065974 1106 82-83 -0.03913189205668388 1106 84-85 -0.2720256448167184 1106 86-87 0.30897736263406017 1106 88-89 0.3177464102043892 1106 90-91 0.007562729471455754 1106 92-93 0.3214776392986849 1106 94-95 0.31848444741252635 1106 96-97 0.4749450048811745 1106 98-99 0.3836443065453956 1106 100-101 -0.24145672983436484 1107 1 0.16994446951103015 1107 2 0.12705265500066787 1107 3 0.08771609373253142 1107 4 -0.05944096318739156 1107 5 -0.008482611865211709 1107 6 -0.012826777313044602 1107 7 0.06145746652331496 1107 8 0.12016358759095169 1107 9 0.07014014400398594 1107 10-11 0.14778824817788916 1107 12-13 0.16008764402998565 1107 14-15 0.14007345961449147 1107 16-17 0.1776312388631638 1107 18-19 0.07498427320586387 1107 20-21 0.10458732350247146 1107 22-23 0.06492802989698987 1107 24-25 -0.023199640938329935 1107 26-27 -0.0019914092442832043 1107 28-29 0.006622722496423705 1107 30-31 0.023997086030988157 1107 32-33 -0.15402008379023613 1107 34-35 -0.10456758909811015 1107 36-37 -0.16307424949615523 1107 38-39 -0.11747241861213809 1107 40-41 -0.2232683767787691 1107 42-43 -0.09917267201990398 1107 44-45 -0.3732667485830774 1107 46-47 -0.365254254455607 1107 48-49 -0.6364852799505059 1107 50-51 -0.45412497871204494 1107 52-53 -0.5354195205601471 1107 54-55 -0.5229358287333525 1107 56-57 -0.37772824779227676 1107 58-59 -0.42285270668719477 1107 60-61 -0.6946080085115085 1107 62-63 -0.601065400485119 1107 64-65 -0.44735530506067533 1107 66-67 -0.5269208327412684 1107 68-69 -0.3578752329192554 1107 70-71 -0.3918207966358018 1107 72-73 -0.5196335366548723 1107 74-75 -0.6731001205913536 1107 76-77 -0.5219597258834092 1107 78-79 -0.5507396916828178 1107 80-81 -0.4020105181102558 1107 82-83 -0.40213463926950155 1107 84-85 -0.32766241469248314 1107 86-87 -0.40434796924295213 1107 88-89 -0.3104745047441462 1107 90-91 -0.416648043838844 1107 92-93 -0.6683952579829651 1107 94-95 -0.5687832698259747 1107 96-97 -0.33153119113235974 1107 98-99 -0.6152291908454792 1107 100-101 -0.40237944448762164 1108 1 0.2593984408910188 1108 2 0.04958248993429493 1108 3 0.07706980139702324 1108 4 0.09077720507625742 1108 5 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-0.23764615478903295 2316 64-65 0.02405316080353259 2316 66-67 0.02760281769810291 2316 68-69 0.11073063912406411 2316 70-71 0.0817353090585371 2316 72-73 0.06896396822427775 2316 74-75 -0.21833459412004075 2316 76-77 -0.011748536222949468 2316 78-79 -0.20747068938591084 2316 80-81 -0.02182851198110569 2316 82-83 -0.0759441969267769 2316 84-85 -0.14794507049537486 2316 86-87 0.036977292078614 2316 88-89 0.011994377325546424 2316 90-91 0.08105504774707484 2316 92-93 -9.645274089820077E-5 2316 94-95 0.03966695671702425 2316 96-97 0.07853804105847928 2316 98-99 -0.1033029356185402 2316 100-101 0.5767483395745536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 16.0 6 52.0 7 107.0 8 268.0 9 468.0 10 791.0 11 1297.0 12 2165.0 13 3217.0 14 4214.0 15 6011.0 16 8456.0 17 12892.0 18 11584.0 19 6236.0 20 4751.0 21 4755.0 22 4929.0 23 5220.0 24 5654.0 25 6502.0 26 7583.0 27 9165.0 28 11648.0 29 14187.0 30 17672.0 31 22352.0 32 28219.0 33 37851.0 34 52667.0 35 77243.0 36 116541.0 37 170308.0 38 126518.0 39 10212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.24113606878161 20.16657255431041 14.245957905880063 20.346333471027922 2 16.437620853715064 22.25501823146573 39.09263251755892 22.21472839726029 3 20.29218339326288 27.069085067122355 29.854009343705123 22.784722195909634 4 12.82744962823471 17.93945915633845 36.380330982882505 32.852760232544334 5 12.834704959331312 39.490952827352 32.684032454094535 14.990309759222153 6 30.314248076760343 36.48920194553168 16.959241591233738 16.237308386474236 7 27.159642468769064 34.90867848080802 20.05297039644439 17.878708653978524 8 27.80390091479395 33.404440470191204 19.237953781624046 19.5537048333908 9 27.98135300588686 15.880004496294978 20.419999419018065 35.71864307880009 10-11 25.613535868687983 27.42443130078066 25.64466892239218 21.31736390813918 12-13 26.102612875805352 25.472791963957548 27.59989794539321 20.824697214843894 14-15 23.04338545751696 25.91703577964618 27.34713544330816 23.692443319528696 16-17 20.735813620995174 29.61536469877981 27.832192209032247 21.81662947119277 18-19 20.678978431677155 26.65033602226929 32.44158921293367 20.229096333119887 20-21 21.518939379587074 25.60602099365593 32.9651676069733 19.9098720197837 22-23 21.25366272607861 25.964938870364758 32.65345559260382 20.127942810952813 24-25 20.87119724260225 26.113435746393165 32.93298103846995 20.08238597253464 26-27 20.477065865578624 26.34332651515965 32.83749486110945 20.342112758152286 28-29 20.318013837561477 26.60996162968988 32.942199510456476 20.129825022292163 30-31 20.45100617230156 26.25848896702675 32.922983266457265 20.36752159421443 32-33 20.30898134921687 26.436951461485318 32.70738844831092 20.546678740986888 34-35 20.79814287861393 26.43032068939822 32.78234774800035 19.989188683987507 36-37 20.98847761261893 26.16218489623789 32.57212069773404 20.27721679340914 38-39 20.663127728878464 26.569945804489475 32.50183451361077 20.265091953021294 40-41 20.748208889716864 26.598737618525874 32.45602346678118 20.19703002497608 42-43 20.655153335640716 26.767736736132342 31.95896733707062 20.618142591156328 44-45 20.723120428714147 27.061885943142077 31.725718428368083 20.48927519977569 46-47 21.146163687871244 27.438766398530873 30.85872919042693 20.556340723170948 48-49 21.119830050153897 27.685620570802122 30.395964448957578 20.798584930086403 50-51 21.382282324382423 28.091929024215577 29.97102668348988 20.554761967912118 52-53 21.457683675544324 28.12243057581625 29.657422738875145 20.762463009764286 54-55 20.958281602655347 28.486500345432525 29.707828498751514 20.847389553160614 56-57 21.19977821646124 28.664890882751532 29.43090293433767 20.704427966449558 58-59 21.112062574280436 28.100833203875403 29.643845443649177 21.14325877819499 60-61 20.967953794254086 28.352549942343856 30.03878685919903 20.640709404203026 62-63 20.95349239608317 28.24481568348104 30.247308853785793 20.554383066649994 64-65 20.787091591802092 28.305755636472025 30.05880547588104 20.848347295844842 66-67 20.79403811494096 28.457063540478657 29.82148698537315 20.927411359207237 68-69 20.968711596778327 28.357286208120357 29.773366525083894 20.90063567001742 70-71 21.007043774462815 28.654723698884645 29.345334399308886 20.99289812734366 72-73 20.961386172377342 28.728925196049826 29.041392436878205 21.268296194694624 74-75 20.844621433433993 29.129108079059012 28.815756735285685 21.210513752221306 76-77 20.86962891673392 28.774898549187068 29.153357759834698 21.20211477424431 78-79 21.096843373585276 28.813483327712962 28.883011709312008 21.206661589389757 80-81 21.072088491126763 29.098353926616934 28.66053351823715 21.16902406401916 82-83 20.924549010494356 29.078545365445795 28.69654781316032 21.300357810899527 84-85 20.78835459600916 28.9244382473038 28.71730555734481 21.569901599342227 86-87 20.82138215602396 28.82529241704904 28.727851642473823 21.625473784453174 88-89 20.750969671479975 28.805463250998088 28.622959143076905 21.82060793444503 90-91 20.577410979158206 28.902507343797073 28.66540896312722 21.8546727139175 92-93 20.583251920901848 28.727484226407185 28.796445344840325 21.89281850785064 94-95 20.391392373727996 28.637483691458176 28.881622404684233 22.089501530129596 96-97 20.46704632573131 28.67070070210404 28.65844956129549 22.20380341086916 98-99 20.221723328944336 28.853840057341152 28.354694175263102 22.56974243845141 100-101 20.051452363928522 28.78458475554758 28.75432980548404 22.409633075039856 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1252.0 1 857.5 2 564.5 3 743.5 4 674.0 5 596.5 6 1092.5 7 2172.0 8 3226.0 9 3687.0 10 3300.0 11 2716.5 12 2583.0 13 2709.0 14 2882.0 15 2835.0 16 2800.0 17 2942.0 18 2999.5 19 3044.5 20 3181.0 21 3451.0 22 3893.0 23 4470.5 24 5084.5 25 5885.0 26 6950.5 27 8084.5 28 9205.5 29 10248.5 30 11452.0 31 12593.0 32 14048.5 33 15142.0 34 16051.0 35 16968.5 36 18308.5 37 20016.0 38 20994.5 39 21573.5 40 21855.0 41 22879.5 42 23873.5 43 24911.0 44 26348.0 45 27487.5 46 27538.5 47 27320.5 48 28517.5 49 28189.0 50 26632.5 51 25433.0 52 24103.0 53 23497.5 54 23213.0 55 21823.5 56 19393.0 57 17459.0 58 16375.5 59 14838.5 60 12149.5 61 9843.5 62 7912.5 63 5669.5 64 3885.5 65 2794.5 66 2002.5 67 1508.5 68 1090.5 69 709.0 70 494.5 71 344.5 72 259.5 73 208.0 74 164.0 75 123.5 76 87.0 77 57.5 78 33.5 79 19.5 80 16.5 81 16.5 82 11.5 83 7.0 84 4.5 85 2.0 86 3.0 87 2.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019702865630245415 2 0.0 3 0.0 4 0.0 5 0.030943603073141833 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003789012621201041 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.789012621201041E-4 24-25 2.526008414134028E-4 26-27 8.20952734593559E-4 28-29 6.315021035335068E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.946523138868576E-4 42-43 0.012756342491376839 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.526008414134028E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.052016828268056E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0137035956466771 92-93 0.001578755258833767 94-95 0.0 96-97 0.0 98-99 0.0013261544174203643 100-101 0.019829166050952113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 791763.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.29351716815617 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04387405459363 42.600267727389344 2 12.776800881427807 14.129485177826826 3 4.187499424675973 6.946247139898952 4 1.8963532460001078 4.194241630583825 5 1.0671859363166791 2.9504231945670556 6 0.6665473597181617 2.2113448726779206 7 0.43967618146536025 1.701786974179897 8 0.3073091896808751 1.3593764764441303 9 0.22840397915183502 1.1366333408256457 >10 1.2756664179063937 12.645665611214934 >50 0.07516420760832182 2.8702297986973444 >100 0.032081787122232766 3.2105324559683193 >500 0.0018332449773803214 0.6710466446120225 >1k 9.166224886901607E-4 0.7336404970361802 >5k 6.874668665176205E-4 2.6390784580776443 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8458 1.0682489583372803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7362 0.9298236972427355 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5008 0.6325125068991605 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1703 0.21508961646351243 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1695 0.21407921309785882 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1381 0.1744208809959546 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1011 0.1276897253344751 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 803 0.1014192378274812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.2630042070670136E-4 0.0 0.0 0.0 0.0 14-15 1.2630042070670136E-4 0.0 0.0 1.2630042070670136E-4 0.0 16-17 1.2630042070670136E-4 0.0 0.0 1.2630042070670136E-4 0.0 18-19 1.8945063106005206E-4 0.0 0.0 1.2630042070670136E-4 0.0 20-21 2.526008414134027E-4 0.0 0.0 2.526008414134027E-4 0.0 22-23 2.526008414134027E-4 0.0 0.0 5.052016828268055E-4 0.0 24-25 3.789012621201041E-4 0.0 0.0 8.20952734593559E-4 0.0 26-27 3.789012621201041E-4 0.0 0.0 0.0018313561002471699 0.0 28-29 3.789012621201041E-4 0.0 0.0 0.004357364514381198 0.0 30-31 3.789012621201041E-4 0.0 0.0 0.008904179659822447 0.0 32-33 3.789012621201041E-4 0.0 0.0 0.014524548381270657 0.0 34-35 3.789012621201041E-4 0.0 0.0 0.020650118785545672 0.0 36-37 3.789012621201041E-4 0.0 0.0 0.030185800548901628 0.0 38-39 3.789012621201041E-4 0.0 0.0 0.041615988622858105 0.0 40-41 3.789012621201041E-4 0.0 0.0 0.057214090580135726 0.0 42-43 3.789012621201041E-4 0.0 0.0 0.07647490473790769 0.0 44-45 3.789012621201041E-4 0.0 0.0 0.09327286069189897 0.0 46-47 5.052016828268055E-4 0.0 0.0 0.12194305619232017 0.0 48-49 5.052016828268055E-4 0.0 0.0 0.1616013882942244 0.0 50-51 5.052016828268055E-4 0.0 0.0 0.18143055434517652 0.0 52-53 5.052016828268055E-4 0.0 0.0 0.21332141057361861 0.0 54-55 5.052016828268055E-4 0.0 0.0 0.2637152784355925 0.0 56-57 5.052016828268055E-4 0.0 0.0 0.34555795105353493 0.0 58-59 5.052016828268055E-4 0.0 0.0 0.45531301664765844 0.0 60-61 5.052016828268055E-4 0.0 0.0 0.5714462534874705 0.0 62-63 5.052016828268055E-4 0.0 0.0 0.6903580995828298 0.0 64-65 5.052016828268055E-4 0.0 0.0 0.817921524496598 0.0 66-67 5.052016828268055E-4 0.0 0.0 0.9795860630011759 0.0 68-69 5.052016828268055E-4 0.0 0.0 1.1774356720382235 0.0 70-71 5.683518931801562E-4 0.0 0.0 1.3061989509487058 0.0 72-73 6.946523138868575E-4 0.0 0.0 1.4824511880449074 0.0 74-75 7.578025242402082E-4 0.0 0.0 1.671964969315313 0.0 76-77 7.578025242402082E-4 0.0 0.0 1.8809921655849036 0.0 78-79 7.578025242402082E-4 0.0 0.0 2.1177423041996155 0.0 80-81 8.841029449469096E-4 0.0 0.0 2.438355922163577 0.0 82-83 8.841029449469096E-4 0.0 0.0 2.8096160088309254 0.0 84-85 9.472531553002602E-4 0.0 0.0 3.2545092407702807 0.0 86-87 0.001010403365653611 0.0 0.0 3.7881285182560944 0.0 88-89 0.001010403365653611 0.0 0.0 4.41167369528508 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4035 0.0 46.996315 1 CGTATGA 50 0.0016141199 38.006668 2 GTATCAA 6450 0.0 35.29476 1 TAAGGTG 320 0.0 28.208075 5 TATCAAC 8160 0.0 27.887861 2 ATCAACG 8235 0.0 27.633873 3 TCAACGC 8310 0.0 27.441639 4 CAACGCA 8420 0.0 27.083138 5 AGGTAAG 370 0.0 26.964191 2 AACGCAG 8645 0.0 26.371592 6 GTAAGGT 330 0.0 25.91364 4 GTACATG 5970 0.0 24.758282 1 GGTAAGG 365 0.0 24.730366 3 GGTGTGC 505 0.0 24.458193 8 TACCTGG 565 0.0 24.384811 2 TACATGG 6055 0.0 23.852245 2 CCTAGAC 160 1.8555038E-6 23.75417 3 GGGCGTA 100 0.0016521119 23.752668 9 GAGTACT 6120 0.0 23.092873 12-13 ACATGGG 5995 0.0 22.902267 3 >>END_MODULE