FastQCFastQC Report
Thu 26 May 2016
SRR937770_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937770_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743101
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84001.1303981558361516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64530.868388011858415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43240.5818859078375618No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15250.20522109376787273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14620.1967431075991016No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8740.117615236690571No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8740.117615236690571No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.10227411886136609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32300.048.856751
TATCACG459.603445E-442.239552
TACCGTT704.3039636E-640.720017
GTATCAA55450.039.8602871
ACTGCGC753.074867E-431.6711168
ATCAACG71050.031.031053
TATCAAC71750.030.7303752
TCAACGC72150.030.6958754
CAACGCA73500.030.1320725
AACGCAG74900.029.620326
GTACATG51300.028.4452671
TACATGG51150.028.0564772
ACATGGG50500.027.0983983
ACGCAGA84400.026.2299887
CGCAGAG84950.025.9483188
CATGGGA35600.025.097954
GCAGAGT88800.024.554169
GAGTACT54500.023.88167412-13
CATGGGG16450.022.8239924
ATGGGAT13600.022.7145865
Produced by FastQC (version 0.11.5)