FastQCFastQC Report
Thu 26 May 2016
SRR937770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937770_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743101
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80381.0816833781679744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68100.9164299334814514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48030.646345516962028No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16390.22056221159707765No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15640.21046937091996915No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12910.17373143085529424No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.13134150001143854No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7490.10079383556205684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.049.5148851
GTATCAA59400.037.760421
GTATACG953.48372E-530.0069084
TATCAAC77600.028.7147272
ATCAACG79450.027.9265083
TCAACGC79950.027.7518564
CAACGCA81550.027.2656275
AACGCAG84300.026.4799866
GTACATG55100.025.873251
TACATGG54350.025.7004892
ACATGGG53300.024.9588413
CATGGGG16350.023.5375294
ATCTAGG2853.6379788E-1223.343421
TGTATAC1852.5334884E-723.1134283
ACGCAGA96200.023.1087637
CGCAGAG96650.023.001178
GTGGTAT18200.022.7158581
ATACGTG1050.002196852622.6166446
GAGTACA44350.022.2869681
GAGTACT61900.022.13915612-13
Produced by FastQC (version 0.11.5)