##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937770_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 743101 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22660580459453 33.0 31.0 34.0 31.0 34.0 2 32.42310533830529 34.0 31.0 34.0 31.0 34.0 3 32.48336901713226 34.0 31.0 34.0 31.0 34.0 4 36.111738511992314 37.0 35.0 37.0 35.0 37.0 5 36.04089888184782 37.0 35.0 37.0 35.0 37.0 6 36.09595061774913 37.0 35.0 37.0 35.0 37.0 7 36.041766866146055 37.0 35.0 37.0 35.0 37.0 8 36.03471129765671 37.0 35.0 37.0 35.0 37.0 9 37.69938945042464 39.0 37.0 39.0 35.0 39.0 10-11 37.63173915793412 39.0 37.0 39.0 35.0 39.0 12-13 37.533276095712424 39.0 37.0 39.0 35.0 39.0 14-15 38.695406815493456 40.0 38.0 41.0 34.5 41.0 16-17 38.670879194079944 40.0 38.0 41.0 34.5 41.0 18-19 38.71725310556708 40.0 38.0 41.0 35.0 41.0 20-21 38.64011554284007 40.0 38.0 41.0 34.0 41.0 22-23 38.482134326289426 40.0 38.0 41.0 34.0 41.0 24-25 38.52774050902906 40.0 38.0 41.0 34.0 41.0 26-27 38.37167222221475 40.0 38.0 41.0 34.0 41.0 28-29 38.20951122391169 40.0 38.0 41.0 34.0 41.0 30-31 38.165571032739834 40.0 37.5 41.0 34.0 41.0 32-33 38.01153140690162 40.0 37.0 41.0 33.5 41.0 34-35 37.85267749606042 40.0 37.0 41.0 33.0 41.0 36-37 37.65309089881457 40.0 36.0 41.0 33.0 41.0 38-39 37.47524562609928 40.0 36.0 41.0 33.0 41.0 40-41 37.248411723305445 40.0 36.0 41.0 33.0 41.0 42-43 36.96250778830872 40.0 35.5 41.0 32.0 41.0 44-45 36.52598099047101 39.0 35.0 41.0 31.0 41.0 46-47 36.000058538475926 39.0 35.0 41.0 29.5 41.0 48-49 35.51336359391254 39.0 35.0 41.0 27.5 41.0 50-51 35.19032877092077 39.0 35.0 40.5 26.0 41.0 52-53 34.922096727093624 39.0 35.0 40.0 25.5 41.0 54-55 34.67544384949018 38.0 34.5 40.0 24.0 41.0 56-57 34.40295599117751 38.0 34.0 40.0 23.5 41.0 58-59 34.12299135649124 38.0 34.0 40.0 23.0 41.0 60-61 33.757771823749394 37.5 34.0 40.0 22.0 41.0 62-63 33.401077377099476 37.0 33.0 40.0 20.5 41.0 64-65 33.00366908401415 36.0 33.0 39.0 19.5 41.0 66-67 32.56634898889922 36.0 32.0 39.0 17.5 40.0 68-69 32.117482684049676 35.0 32.0 39.0 14.0 40.0 70-71 31.69134545640498 35.0 31.0 38.0 9.5 40.0 72-73 31.209302638537693 35.0 31.0 37.0 7.0 39.5 74-75 30.712450259116864 35.0 30.5 37.0 4.5 39.0 76-77 29.48281525660711 33.5 29.5 35.5 2.0 38.0 78-79 30.078288146564198 34.0 30.0 36.0 2.0 38.0 80-81 29.878190178724026 34.0 30.0 35.5 2.0 37.0 82-83 29.52306954236369 34.0 30.0 35.0 2.0 37.0 84-85 29.157241074900988 34.0 29.0 35.0 2.0 36.5 86-87 28.74365664963444 34.0 29.0 35.0 2.0 36.0 88-89 28.400406539622473 34.0 29.0 35.0 2.0 36.0 90-91 27.878212382973512 34.0 27.5 35.0 2.0 35.0 92-93 27.419599758310106 33.0 27.0 35.0 2.0 35.0 94-95 26.965687705978056 33.0 26.0 35.0 2.0 35.0 96-97 26.429622621958522 33.0 25.0 35.0 2.0 35.0 98-99 25.587302399000944 32.5 22.0 34.5 2.0 35.0 100-101 23.08582144284559 29.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3198627908650309 1101 2 0.2686533652185119 1101 3 0.3107514800257931 1101 4 0.18343581264523579 1101 5 0.09429820119324006 1101 6 0.08740711673399204 1101 7 0.11886385252246612 1101 8 0.1602854246909402 1101 9 0.1904866627079116 1101 10-11 0.2695043701384918 1101 12-13 0.24126597791596538 1101 14-15 0.2792778095793551 1101 16-17 0.23771295729474673 1101 18-19 0.1697941789805597 1101 20-21 0.12310890023032783 1101 22-23 0.2355105458181299 1101 24-25 0.19018816989830611 1101 26-27 0.07907670367956854 1101 28-29 0.06371950714979135 1101 30-31 0.41371895963665395 1101 32-33 0.47941491802109226 1101 34-35 0.36691635832536207 1101 36-37 0.3252559341608716 1101 38-39 0.31285672284233357 1101 40-41 0.47345456167949607 1101 42-43 0.3862111112022859 1101 44-45 0.5573811078331659 1101 46-47 0.5758370968678648 1101 48-49 0.831811120744149 1101 50-51 0.67024017329868 1101 52-53 0.6045925670627952 1101 54-55 0.8348325817854132 1101 56-57 0.8374514660046302 1101 58-59 0.6531768841553003 1101 60-61 0.6646402208373559 1101 62-63 0.7304831337939603 1101 64-65 0.809399504401739 1101 66-67 0.8096393604797711 1101 68-69 0.7110365798241851 1101 70-71 0.9127650473676674 1101 72-73 0.8936449390496612 1101 74-75 0.8185479242504385 1101 76-77 0.6131732174213163 1101 78-79 0.8054144314102132 1101 80-81 0.9016886820899082 1101 82-83 0.9010094581197094 1101 84-85 0.7920901819511066 1101 86-87 0.6544313142699139 1101 88-89 1.1031855787024654 1101 90-91 1.0365239970787208 1101 92-93 1.2638497790487193 1101 94-95 1.1550826493869444 1101 96-97 1.2024369867977747 1101 98-99 1.1296174871014024 1101 100-101 1.7389065048192869 1102 1 0.28386914388823214 1102 2 0.23559434541955682 1102 3 0.14033767980809841 1102 4 0.09251039130391092 1102 5 0.08436137571284519 1102 6 0.15773590169162333 1102 7 0.13018617774318386 1102 8 0.15376877379419085 1102 9 0.24870788735450589 1102 10-11 0.19352820642541957 1102 12-13 0.20185437955793617 1102 14-15 0.22631534790737362 1102 16-17 0.28084486256142327 1102 18-19 0.2197713244598134 1102 20-21 0.0933495574445331 1102 22-23 0.18223905901150061 1102 24-25 0.24391941496280367 1102 26-27 0.32077348732818933 1102 28-29 0.27008071173420944 1102 30-31 0.19475264204240972 1102 32-33 0.1316162441168629 1102 34-35 0.03157739999351605 1102 36-37 0.0736988031946808 1102 38-39 0.13603876576970464 1102 40-41 0.10678178861689247 1102 42-43 0.24463446941177125 1102 44-45 0.3667721815865761 1102 46-47 0.03440371824049038 1102 48-49 -0.056549881084208664 1102 50-51 -0.3174797458834675 1102 52-53 -0.09801409329590882 1102 54-55 -0.12679538930228773 1102 56-57 -0.22776395341584532 1102 58-59 -0.06910156038559023 1102 60-61 -0.16556669705710902 1102 62-63 -0.11005545032845276 1102 64-65 -0.2022207299415797 1102 66-67 -0.28011132070944456 1102 68-69 -0.2213518332005755 1102 70-71 -0.33017006002190286 1102 72-73 -0.12440968770132699 1102 74-75 -0.14648465900404517 1102 76-77 -0.24747111999322158 1102 78-79 0.08774557474096412 1102 80-81 -0.0506507493394146 1102 82-83 0.09211727098653455 1102 84-85 0.19246848207275846 1102 86-87 0.1575611386191369 1102 88-89 0.4297384411200298 1102 90-91 0.32805608589298885 1102 92-93 0.3997608118240592 1102 94-95 0.3856011713308405 1102 96-97 0.47335420896861535 1102 98-99 0.49830003514036036 1102 100-101 0.7463199955672088 1103 1 0.2113594701601187 1103 2 0.24817583810968102 1103 3 0.08140133820880635 1103 4 0.07972375535227627 1103 5 -0.005205301493290904 1103 6 0.08222247892335588 1103 7 0.14723000688331211 1103 8 0.11279281884289816 1103 9 0.10626318193270379 1103 10-11 0.1456874804064796 1103 12-13 0.18598400751463373 1103 14-15 0.20786656973310613 1103 16-17 0.1450475516319969 1103 18-19 0.06749385784734585 1103 20-21 -0.022811402851779405 1103 22-23 0.12951912285058143 1103 24-25 0.020239860029576562 1103 26-27 0.00639802622635699 1103 28-29 -0.03775086753013568 1103 30-31 0.2061794981184093 1103 32-33 0.16345485471354948 1103 34-35 0.2327081345945956 1103 36-37 0.18370640666969962 1103 38-39 0.06342840245910253 1103 40-41 0.0845152755253622 1103 42-43 0.0025437772697003425 1103 44-45 0.21470926847697314 1103 46-47 0.4480892204462492 1103 48-49 0.39127315368098436 1103 50-51 0.3511988303846536 1103 52-53 0.260053118411939 1103 54-55 0.29002466429844986 1103 56-57 0.24909249899845065 1103 58-59 0.28848572901321035 1103 60-61 0.11916273968348179 1103 62-63 0.3094700476615131 1103 64-65 0.15722978724528502 1103 66-67 0.13214570685880034 1103 68-69 0.2162460333095062 1103 70-71 0.16792624815220591 1103 72-73 0.05946925142569981 1103 74-75 0.10431144741449216 1103 76-77 0.18885918700649285 1103 78-79 0.34080296215683603 1103 80-81 0.25340261802135444 1103 82-83 0.27890209264364074 1103 84-85 0.3533403221137128 1103 86-87 0.4271146791283442 1103 88-89 0.4246418544027719 1103 90-91 0.47908857757433765 1103 92-93 0.4194178649881586 1103 94-95 0.4366968998340859 1103 96-97 0.8383878223507324 1103 98-99 0.9009736729138211 1103 100-101 1.076338100871574 1104 1 0.24566184559962068 1104 2 0.1918341864503077 1104 3 0.12076331402563056 1104 4 0.02316771384639793 1104 5 0.007572333357927619 1104 6 0.19229022611484936 1104 7 0.022069214498444012 1104 8 0.09491270564505783 1104 9 0.053308889847976104 1104 10-11 0.24775848632363306 1104 12-13 0.21741355901107795 1104 14-15 0.3287395651880445 1104 16-17 0.1727895977686913 1104 18-19 0.15582247460405796 1104 20-21 0.12745444055787658 1104 22-23 0.15233020251785234 1104 24-25 0.16660380823299192 1104 26-27 0.2741709445009519 1104 28-29 -0.0019477631651554361 1104 30-31 0.32973236665456085 1104 32-33 0.347251270115315 1104 34-35 0.21261980597783747 1104 36-37 0.24609385200029266 1104 38-39 0.2683207787561699 1104 40-41 0.24627241470724925 1104 42-43 0.2615052728532703 1104 44-45 0.36188789809707345 1104 46-47 0.4799261126510359 1104 48-49 0.6848586094737996 1104 50-51 0.6376665432737738 1104 52-53 0.5338503913730932 1104 54-55 0.4606361041406615 1104 56-57 0.38164974108746463 1104 58-59 0.4747905056189978 1104 60-61 0.36740474294220604 1104 62-63 0.474400156571555 1104 64-65 0.30820311714838056 1104 66-67 0.3122331779161698 1104 68-69 0.2859227334209855 1104 70-71 0.3417106577225688 1104 72-73 0.42541128041120757 1104 74-75 0.18132028027616798 1104 76-77 0.035999226454226374 1104 78-79 0.18324786396628667 1104 80-81 0.6026651169494031 1104 82-83 0.7831255726196318 1104 84-85 0.7095919300375613 1104 86-87 0.7895835926006036 1104 88-89 0.7369435437947622 1104 90-91 0.6017195697705446 1104 92-93 0.7695808012714984 1104 94-95 0.7098510032557463 1104 96-97 0.9337698650571902 1104 98-99 1.0597293542447552 1104 100-101 1.2218230507043515 1105 1 0.1369279456983108 1105 2 0.0470634031995516 1105 3 0.08743386816622944 1105 4 0.019641398782937358 1105 5 -0.044020715838684055 1105 6 -0.029279485697927043 1105 7 0.07172354282445781 1105 8 0.05983013444471652 1105 9 0.030392858030886316 1105 10-11 0.05334528797342841 1105 12-13 0.05839455678769667 1105 14-15 -0.010299576658354681 1105 16-17 -0.06875671395906124 1105 18-19 0.0304777747985554 1105 20-21 0.05179040357921849 1105 22-23 -0.037400470780099226 1105 24-25 0.0554571664461605 1105 26-27 -0.09325741625161044 1105 28-29 -0.09720090668944437 1105 30-31 -0.1293952340341491 1105 32-33 -0.025476595423235437 1105 34-35 -0.007957972970366711 1105 36-37 -0.21320366891996656 1105 38-39 -0.2765093198226509 1105 40-41 0.00973153917288272 1105 42-43 -0.019750958077736414 1105 44-45 0.005345091365981602 1105 46-47 -0.05087522112872733 1105 48-49 0.21176933426195887 1105 50-51 0.30391812804641205 1105 52-53 0.21761630023314638 1105 54-55 0.283240009421732 1105 56-57 0.28815473899950916 1105 58-59 0.3280521589491201 1105 60-61 0.46284222797229546 1105 62-63 0.17949387604391376 1105 64-65 0.35586730962599233 1105 66-67 0.3273309980465129 1105 68-69 0.044632869570353506 1105 70-71 0.08515368052197658 1105 72-73 0.14340827827811253 1105 74-75 0.19030661517828307 1105 76-77 -0.14614146749749324 1105 78-79 0.1583943653024491 1105 80-81 -0.008458325396226485 1105 82-83 0.031592214958234166 1105 84-85 -0.1802495989686399 1105 86-87 -0.07515112492425757 1105 88-89 -0.24260332722763067 1105 90-91 -0.30837068232586873 1105 92-93 -0.15018863390362114 1105 94-95 -0.1728432168229297 1105 96-97 -0.05182579000097576 1105 98-99 0.030688042937097038 1105 100-101 0.23722183655845797 1106 1 0.13146876571048693 1106 2 0.06602089589828353 1106 3 0.1262673167374544 1106 4 0.022499986413613726 1106 5 0.06555503673352803 1106 6 0.04276311448163739 1106 7 0.123498034315773 1106 8 0.011812054135951655 1106 9 -0.001392159667588544 1106 10-11 0.017963816161994828 1106 12-13 -0.04099038248022424 1106 14-15 0.20253104428466884 1106 16-17 0.2847742093544241 1106 18-19 0.24104319724001755 1106 20-21 0.1432548879498654 1106 22-23 -0.03391753458748781 1106 24-25 0.15014659173588285 1106 26-27 0.2267805606526494 1106 28-29 0.48443395775984044 1106 30-31 0.3570858136330557 1106 32-33 0.3655049217953703 1106 34-35 0.23218918753365614 1106 36-37 0.07763440292655588 1106 38-39 0.09557662356287011 1106 40-41 0.0711137746503212 1106 42-43 0.2072900425311417 1106 44-45 0.1267112279383511 1106 46-47 0.3244150235539891 1106 48-49 0.21971558868658292 1106 50-51 0.0731000766331178 1106 52-53 0.09762785842140431 1106 54-55 0.1225811926912499 1106 56-57 -0.05112968530983153 1106 58-59 -0.016092896313466554 1106 60-61 0.06253676156844534 1106 62-63 0.13839006906565743 1106 64-65 -0.004371490799179867 1106 66-67 0.09361307975877509 1106 68-69 0.13702097702023153 1106 70-71 0.15681444769672126 1106 72-73 0.17592084264793684 1106 74-75 -0.25798081219604896 1106 76-77 -0.043897940598906615 1106 78-79 0.10154768673359627 1106 80-81 0.2788297314865744 1106 82-83 0.15433088638577175 1106 84-85 0.10453590376095079 1106 86-87 0.2724820264364105 1106 88-89 0.044364815515990585 1106 90-91 0.10836908300163373 1106 92-93 0.4836283545691522 1106 94-95 0.7323228805341664 1106 96-97 0.7392960766464256 1106 98-99 1.0904345883803828 1106 100-101 1.1892863147372204 1107 1 0.40297389787507853 1107 2 0.27824985142817127 1107 3 0.3434748828413774 1107 4 0.16420439276512866 1107 5 0.13276411675030886 1107 6 0.2197525041152275 1107 7 0.22040359643161622 1107 8 0.19643372731243858 1107 9 0.23764170440033894 1107 10-11 0.3698706975447621 1107 12-13 0.3582846695335036 1107 14-15 0.5923354899979145 1107 16-17 0.43830335650242347 1107 18-19 0.47501626689298604 1107 20-21 0.4436238501409804 1107 22-23 0.41333774139882706 1107 24-25 0.37008460781795804 1107 26-27 0.4319990182782263 1107 28-29 0.3545099561853746 1107 30-31 0.37483319780790936 1107 32-33 0.3109835129048122 1107 34-35 0.4173947985272619 1107 36-37 0.4055803692138866 1107 38-39 0.35443842062068853 1107 40-41 0.49536730747411895 1107 42-43 0.20546037070418777 1107 44-45 0.378334358796657 1107 46-47 0.26411046754137857 1107 48-49 0.5073168586431933 1107 50-51 0.6470763471304153 1107 52-53 0.6570056834909366 1107 54-55 0.8262111231810252 1107 56-57 0.6450442243484957 1107 58-59 0.5393401043915844 1107 60-61 0.6814686744189729 1107 62-63 0.7961906132091912 1107 64-65 0.9500510812090042 1107 66-67 1.0645621733575226 1107 68-69 0.9410492828652259 1107 70-71 0.7942615148149663 1107 72-73 0.559405783540452 1107 74-75 0.7313936822651961 1107 76-77 0.5787765811127663 1107 78-79 0.6922051142063594 1107 80-81 0.6045167235147417 1107 82-83 0.5627402397719621 1107 84-85 0.5210371213794396 1107 86-87 0.778443599002582 1107 88-89 0.6183800152010974 1107 90-91 0.8914394280439986 1107 92-93 1.0963272719051815 1107 94-95 1.2206571005878892 1107 96-97 1.1645639310858655 1107 98-99 1.4546430931102812 1107 100-101 1.5867468340525832 1108 1 0.21157361498964633 1108 2 0.1787731894762672 1108 3 -0.01978772913410154 1108 4 0.07074376098766777 1108 5 0.08370706818831053 1108 6 0.01351016559566176 1108 7 0.08423197926990156 1108 8 0.03774914456845124 1108 9 0.05271006837958936 1108 10-11 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-0.6707002981479064 2316 80-81 -0.6016814458772686 2316 82-83 -0.7216748164645352 2316 84-85 -0.6011732582987577 2316 86-87 -0.5813601575770413 2316 88-89 -0.5876101305556851 2316 90-91 -0.6837646221060574 2316 92-93 -0.8590364656017151 2316 94-95 -0.691369937810002 2316 96-97 -0.5597954028645233 2316 98-99 -0.2564331136605382 2316 100-101 0.26966271770893613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 12.0 5 17.0 6 49.0 7 107.0 8 249.0 9 454.0 10 794.0 11 1320.0 12 2022.0 13 3061.0 14 4006.0 15 5714.0 16 8129.0 17 11779.0 18 10975.0 19 5941.0 20 4601.0 21 4625.0 22 4628.0 23 4819.0 24 5469.0 25 6253.0 26 7325.0 27 8995.0 28 10983.0 29 13557.0 30 17007.0 31 21278.0 32 27108.0 33 35770.0 34 49837.0 35 72433.0 36 107981.0 37 156468.0 38 118853.0 39 10477.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.29740352388515 20.197326800640706 14.30794287483343 20.197326800640706 2 16.345019048554636 22.310291602352844 39.23396684972837 22.11072249936415 3 20.35766335935492 27.060924423463295 29.921773756191953 22.659638460989825 4 12.818463194724803 17.87861660610954 36.6003229713363 32.70259722782936 5 12.872329256835513 39.610583639990416 32.5941708683398 14.922916234834272 6 30.177459053345373 36.615480264459336 17.001322834984748 16.205737847210543 7 27.1159640479558 34.96186924792188 20.029578751744378 17.89258795237794 8 27.92944700653074 33.31687078876222 19.26790570864526 19.485776496061774 9 28.013823154591368 15.993788192991262 20.395612440300848 35.596776212116524 10-11 25.618993918727064 27.457438490864632 25.672351403106713 21.25121618730159 12-13 26.17242066108807 25.518047903450242 27.521067774850017 20.788463660611672 14-15 23.036101418245973 25.993034594220703 27.441222660176745 23.529641327356575 16-17 20.68123983146302 29.602772705190816 27.91915230904009 21.796835154306077 18-19 20.652979877567116 26.638101684696963 32.51644123746301 20.19247720027291 20-21 21.53140793203611 25.62614343551108 33.00071793788323 19.841730694569577 22-23 21.229956203240114 25.986032675652996 32.7867429515746 19.997268169532283 24-25 20.919161729024722 26.06772161523131 33.01482571009863 19.99829094564534 26-27 20.359051375527017 26.381549111354236 32.947582886218086 20.31181662690066 28-29 20.334105335969177 26.55220303355318 32.988780617126615 20.124911013351024 30-31 20.416807405722775 26.338075174168786 32.933275557427585 20.311841862680847 32-33 20.34292781196634 26.406033634727983 32.807182334568246 20.443856218737427 34-35 20.78829122824488 26.41646290342766 32.82891558482629 19.966330283501165 36-37 20.925688432662586 26.275364990761684 32.659490432659894 20.139456143915837 38-39 20.669035573298057 26.64481674392191 32.505404727092404 20.18074295568763 40-41 20.717096356584765 26.641111353341568 32.524283603420294 20.11750868665337 42-43 20.610207725277988 26.82971803280454 32.06548255563938 20.4945916862781 44-45 20.707548502828015 27.095240081765464 31.808529392370623 20.3886820230359 46-47 21.10735956485054 27.535287935287396 30.827841706578248 20.52951079328382 48-49 21.06819934302336 27.749390728851125 30.376422585893437 20.80598734223208 50-51 21.197724131712917 28.223754240675227 30.05910367500515 20.519417952606712 52-53 21.341648039768486 28.39896595482983 29.605329558162353 20.654056447239338 54-55 20.991359182668305 28.498952363137715 29.691656988753884 20.818031465440097 56-57 21.166167183195824 28.70148203272503 29.460127223621015 20.672223560458132 58-59 21.03973753231391 28.15882363231916 29.641731070204454 21.159707765162477 60-61 20.864660389368336 28.387460116457923 30.0852104895566 20.662669004617136 62-63 20.970164217246374 28.308534102362938 30.320037249310662 20.40126443108003 64-65 20.73103117880342 28.39748567153052 30.067514375569402 20.803968774096656 66-67 20.71542091855616 28.57633080832888 29.803014664224648 20.90523360889031 68-69 20.97972206914696 28.47482534280449 29.725130652262116 20.82032193578644 70-71 20.846425990545026 28.786665608039826 29.377096787650668 20.98981161376448 72-73 20.988869615301283 28.73236612519698 29.062065587315857 21.21669867218588 74-75 20.796029072764 29.283704368585155 28.80052644256972 21.119740116081125 76-77 20.863449248487083 28.763855788109556 29.220926899573545 21.151768063829817 78-79 21.013765288971488 28.81916455502011 28.9071741257245 21.259896030283905 80-81 20.947463966803905 29.13943556683863 28.75839641932204 21.154704047035423 82-83 20.900561917584973 29.090118769097025 28.7283813799447 21.280937933373302 84-85 20.738087179794277 29.024965129024295 28.702866989684384 21.534080701497047 86-87 20.758550991049667 28.911749546831455 28.664071236615214 21.665628225503667 88-89 20.66650428407444 28.7932595972822 28.709018020430598 21.83121809821276 90-91 20.655418664183063 28.930452782346304 28.687520692968832 21.726607860501797 92-93 20.45762386830445 28.779568752922813 28.897995162012037 21.8648122167607 94-95 20.219727870101103 28.811561281710024 28.897013999442876 22.071696848745997 96-97 20.333776969752428 28.807255003021126 28.66790651607251 22.191061511153933 98-99 20.106043682461074 28.890982249794778 28.47562206462205 22.527352003122097 100-101 19.908620668769107 28.86403740778279 28.795189966444447 22.43215195700366 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1138.0 1 792.0 2 531.0 3 687.5 4 622.0 5 559.0 6 1054.5 7 2038.0 8 3010.0 9 3435.5 10 3062.0 11 2610.0 12 2584.5 13 2610.5 14 2649.5 15 2745.5 16 2751.5 17 2778.0 18 2795.5 19 2791.0 20 2909.5 21 3246.5 22 3712.5 23 4325.0 24 4959.5 25 5651.0 26 6642.5 27 7683.5 28 8766.0 29 9905.0 30 10972.5 31 11872.0 32 13010.5 33 14105.0 34 15229.0 35 16138.5 36 17440.5 37 19034.5 38 19902.5 39 20416.0 40 20615.0 41 21452.0 42 22346.0 43 23434.5 44 24751.0 45 25729.0 46 25836.0 47 25752.5 48 26760.5 49 26215.0 50 24937.0 51 23829.0 52 22422.5 53 21860.0 54 21720.0 55 20490.0 56 17944.0 57 15934.5 58 14930.5 59 13597.0 60 11164.5 61 9079.0 62 7471.0 63 5339.5 64 3602.5 65 2600.5 66 1828.0 67 1382.0 68 1030.5 69 699.0 70 472.5 71 313.5 72 244.0 73 212.0 74 155.5 75 109.5 76 81.0 77 51.5 78 31.0 79 25.5 80 18.0 81 13.0 82 8.5 83 4.0 84 4.5 85 3.0 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023011676743807368 2 0.0 3 0.0 4 1.3457120902811326E-4 5 0.019243682891020197 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003095137807646605 14-15 0.0 16-17 0.0 18-19 0.0 20-21 6.728560451405663E-5 22-23 0.0012784264857670761 24-25 0.0 26-27 5.38284836112453E-4 28-29 0.0016148545083373592 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.018568135421699E-4 40-41 9.419984631967929E-4 42-43 0.016754115524000104 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.364280225702832E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.018568135421699E-4 82-83 0.002758709785076322 84-85 6.055704406265097E-4 86-87 0.0 88-89 0.0 90-91 0.01278426485767076 92-93 0.0031624234121606616 94-95 0.0 96-97 0.0 98-99 0.001480283299309246 100-101 0.020723966190329443 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 743101.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.18159578021296 #Duplication Level Percentage of deduplicated Percentage of total 1 77.70054301730835 43.653404997014675 2 12.506174071931952 14.052336329325222 3 4.087464098361249 6.889207672207928 4 1.8253433892345974 4.102028178162484 5 0.9824658962109649 2.7598250924384544 6 0.6061441622973416 2.0432487786435027 7 0.43165672589157605 1.69758145828955 8 0.3035966967160017 1.3645237516085051 9 0.22244107260465645 1.124738498339263 >10 1.2247214607119914 12.326829599354912 >50 0.0745701342745383 2.8372116800285223 >100 0.031752167092188144 3.2149164964633674 >500 0.0016838270424797096 0.6711200689327763 >1k 9.621868814169767E-4 1.2564243416971448 >5k 4.8109344070848835E-4 2.00660305749373 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8038 1.0816833781679744 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6810 0.9164299334814514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4803 0.646345516962028 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1639 0.22056221159707765 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1564 0.21046937091996915 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1291 0.17373143085529424 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 976 0.13134150001143854 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 749 0.10079383556205684 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.728560451405664E-5 0.0 18-19 0.0 0.0 0.0 1.3457120902811328E-4 0.0 20-21 0.0 0.0 0.0 2.0185681354216992E-4 0.0 22-23 0.0 0.0 0.0 3.3642802257028315E-4 0.0 24-25 1.3457120902811328E-4 0.0 0.0 8.747128586827362E-4 0.0 26-27 1.3457120902811328E-4 0.0 0.0 0.001480283299309246 0.0 28-29 1.3457120902811328E-4 0.0 0.0 0.0030278522031325487 0.0 30-31 1.3457120902811328E-4 0.0 0.0 0.008276129355228966 0.0 32-33 1.3457120902811328E-4 0.0 0.0 0.015273832224690857 0.0 34-35 1.3457120902811328E-4 0.0 0.0 0.022742534325751143 0.0 36-37 2.6914241805622656E-4 0.0 0.0 0.03256623258480341 0.0 38-39 5.382848361124531E-4 0.0 0.0 0.04487949821087578 0.0 40-41 5.382848361124531E-4 0.0 0.0 0.059749616808482287 0.0 42-43 6.055704406265097E-4 0.0 0.0 0.08208843750714909 0.0 44-45 6.728560451405663E-4 0.0 0.0 0.1013321203981693 0.0 46-47 6.728560451405663E-4 0.0 0.0 0.1351767794687398 0.0 48-49 6.728560451405663E-4 0.0 0.0 0.1772302822900252 0.0 50-51 6.728560451405663E-4 0.0 0.0 0.19533010990430644 0.0 52-53 6.728560451405663E-4 0.0 0.0 0.2365088998669091 0.0 54-55 6.728560451405663E-4 0.0 0.0 0.2985462272288693 0.0 56-57 8.074272541686796E-4 0.0 0.0 0.39604306816973733 0.0 58-59 8.074272541686796E-4 0.0 0.0 0.5209924357523406 0.0 60-61 8.074272541686796E-4 0.0 0.0 0.6539487902721164 0.0 62-63 8.074272541686796E-4 0.0 0.0 0.7881835712776595 0.0 64-65 8.074272541686796E-4 0.0 0.0 0.9321747649377407 0.0 66-67 8.074272541686796E-4 0.0 0.0 1.1137113259166656 0.0 68-69 8.074272541686796E-4 0.0 0.0 1.3326586830054057 0.0 70-71 9.419984631967929E-4 0.0 0.0 1.4731510252307558 0.0 72-73 9.419984631967929E-4 0.0 0.0 1.6579845808308695 0.0 74-75 9.419984631967929E-4 0.0 0.0 1.8646186722935374 0.0 76-77 9.419984631967929E-4 0.0 0.0 2.0846425990545026 0.0 78-79 9.419984631967929E-4 0.0 0.0 2.3278800593728173 0.0 80-81 0.0010765696722249063 0.0 0.0 2.6822733383483537 0.0 82-83 0.0010765696722249063 0.0 0.0 3.073606414202107 0.0 84-85 0.0010765696722249063 0.0 0.0 3.5601486204432504 0.0 86-87 0.0010765696722249063 0.0 0.0 4.122790845389792 0.0 88-89 0.0010765696722249063 0.0 0.0 4.777143349288993 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3455 0.0 49.514885 1 GTATCAA 5940 0.0 37.76042 1 GTATACG 95 3.48372E-5 30.006908 4 TATCAAC 7760 0.0 28.714727 2 ATCAACG 7945 0.0 27.926508 3 TCAACGC 7995 0.0 27.751856 4 CAACGCA 8155 0.0 27.265627 5 AACGCAG 8430 0.0 26.479986 6 GTACATG 5510 0.0 25.87325 1 TACATGG 5435 0.0 25.700489 2 ACATGGG 5330 0.0 24.958841 3 CATGGGG 1635 0.0 23.537529 4 ATCTAGG 285 3.6379788E-12 23.34342 1 TGTATAC 185 2.5334884E-7 23.113428 3 ACGCAGA 9620 0.0 23.108763 7 CGCAGAG 9665 0.0 23.00117 8 GTGGTAT 1820 0.0 22.715858 1 ATACGTG 105 0.0021968526 22.616644 6 GAGTACA 4435 0.0 22.286968 1 GAGTACT 6190 0.0 22.139156 12-13 >>END_MODULE