FastQCFastQC Report
Thu 26 May 2016
SRR937769_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937769_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663056
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80171.2090984773533457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60870.9180220071909462No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41760.6298110566829951No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13440.20269781134625128No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13300.2005863758113945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8820.1330204386959774No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7770.11718467218455153No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.1040636085036558No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.10240462344055404No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.10074563837745229No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30800.050.0148161
GTATCAA54250.042.067421
GTGTACG651.3202082E-436.5730361
ATAGCGA550.002576435534.552026
ATCAACG67600.033.1795043
TATCAAC67800.033.1592372
TCAACGC68350.032.812954
CAACGCA68550.032.7221575
AACGCAG70250.031.9882856
ACGCAGA81050.027.5499617
CGCAGAG80800.027.5176058
TACATGG47400.026.1718982
GTACATG47900.026.1050571
GCAGAGT84100.025.814499
TACCTGG4100.025.5042112
GAGTACT52200.024.65812912-13
ACATGGG48250.023.7352773
GTACTTT55600.023.19034214-15
GTATTAG1252.240104E-422.8215771
CATGGGG13850.022.3000264
Produced by FastQC (version 0.11.5)