FastQCFastQC Report
Thu 26 May 2016
SRR937769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937769_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663056
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71961.0852778649163872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62970.9496935402137979No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43410.6546958326295215No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.22381216669481915No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.22260563210347242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13140.19817330662870106No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.1312106368089573No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA6640.10014237108177891No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37400.047.7714581
GTATCAA58650.033.946751
TATCAAC73950.026.7184242
ATCAACG74850.026.3971613
GGCTTAC1806.682967E-926.3905667
TCAACGC75550.026.2154464
CAACGCA75700.026.1635025
GGACCGA2207.6397555E-1125.906836
AACGCAG77250.025.6385386
ATGGGAG11550.025.495615
TCGCACG851.12322596E-725.1467622-23
TACATGG49000.024.6202982
GTACATG49000.024.534471
GTGTACA6100.024.1481631
ACATGGG49550.023.4843273
GAGTACT55000.022.93100212-13
ACGCAGA87300.022.5816177
CGCAGAG87350.022.4599278
CTTACTA2554.8930815E-1022.354369
GTACTTT56500.022.23478314-15
Produced by FastQC (version 0.11.5)