##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937769_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 663056 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.282499517386164 33.0 31.0 34.0 31.0 34.0 2 32.474337009242056 34.0 31.0 34.0 31.0 34.0 3 32.5194221905842 34.0 31.0 34.0 31.0 34.0 4 36.1184183538042 37.0 35.0 37.0 35.0 37.0 5 36.0579121522164 37.0 35.0 37.0 35.0 37.0 6 36.12183586303419 37.0 35.0 37.0 35.0 37.0 7 36.07758922323303 37.0 35.0 37.0 35.0 37.0 8 36.056541227286985 37.0 35.0 37.0 35.0 37.0 9 37.73987415844212 39.0 37.0 39.0 35.0 39.0 10-11 37.67074274269443 39.0 37.0 39.0 35.0 39.0 12-13 37.56510159021259 39.0 37.0 39.0 35.0 39.0 14-15 38.74475082044352 40.0 38.0 41.0 34.5 41.0 16-17 38.722508355252046 40.0 38.0 41.0 34.5 41.0 18-19 38.75859354262687 40.0 38.0 41.0 35.0 41.0 20-21 38.69077197099491 40.0 38.0 41.0 34.0 41.0 22-23 38.62123636615912 40.0 38.0 41.0 34.0 41.0 24-25 38.60964307690452 40.0 38.0 41.0 34.0 41.0 26-27 38.40314468159552 40.0 38.0 41.0 34.0 41.0 28-29 38.34435402137979 40.0 38.0 41.0 34.0 41.0 30-31 38.27136923578099 40.0 37.5 41.0 34.0 41.0 32-33 38.05496066697232 40.0 37.0 41.0 34.0 41.0 34-35 37.872108087401365 40.0 37.0 41.0 33.0 41.0 36-37 37.668381705315994 40.0 36.0 41.0 33.0 41.0 38-39 37.48042850076011 40.0 36.0 41.0 33.0 41.0 40-41 37.25242136410801 40.0 36.0 41.0 33.0 41.0 42-43 36.95126806785551 40.0 35.5 41.0 32.0 41.0 44-45 36.517898186578506 39.5 35.0 41.0 31.0 41.0 46-47 35.9882800246133 39.0 35.0 41.0 29.5 41.0 48-49 35.47076265051519 39.0 35.0 41.0 27.0 41.0 50-51 35.152192273352476 39.0 35.0 41.0 26.0 41.0 52-53 34.85705430612196 39.0 35.0 40.0 24.0 41.0 54-55 34.61485153591853 38.0 34.5 40.0 24.0 41.0 56-57 34.334836725706424 38.0 34.0 40.0 23.0 41.0 58-59 34.06125576120267 38.0 34.0 40.0 22.5 41.0 60-61 33.708631699283316 37.5 34.0 40.0 21.5 41.0 62-63 33.299884926763355 37.0 33.0 40.0 19.5 41.0 64-65 32.9072506696267 36.0 33.0 39.0 18.0 41.0 66-67 32.47900478994232 36.0 32.5 39.0 14.0 40.0 68-69 32.007181143070866 35.0 32.0 39.0 8.0 40.0 70-71 31.585531991264688 35.0 31.0 38.0 7.0 40.0 72-73 31.123621534229386 35.0 31.0 37.0 4.0 39.5 74-75 30.66262427306291 35.0 31.0 37.0 2.0 39.0 76-77 29.41579293453343 33.5 29.5 35.5 2.0 38.0 78-79 29.9951233379986 34.0 30.0 36.0 2.0 38.0 80-81 29.822742724596417 34.0 30.0 35.5 2.0 37.0 82-83 29.44219643589682 34.0 30.0 35.0 2.0 37.0 84-85 29.066688937284333 34.0 29.5 35.0 2.0 36.5 86-87 28.75338131319225 34.0 29.0 35.0 2.0 36.0 88-89 28.370700966434207 34.0 29.0 35.0 2.0 36.0 90-91 27.894103213001614 34.0 28.0 35.0 2.0 35.0 92-93 27.476450707029272 33.5 27.0 35.0 2.0 35.0 94-95 27.022850256991866 33.0 26.0 35.0 2.0 35.0 96-97 26.54302276127507 33.0 25.0 35.0 2.0 35.0 98-99 25.80518538403996 33.0 24.0 34.5 2.0 35.0 100-101 23.493931885089644 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23867367532216122 1101 2 0.25908616161821385 1101 3 0.2713583327850557 1101 4 0.2602452655533156 1101 5 0.09849281460981274 1101 6 0.17312908864926868 1101 7 0.16317609631970953 1101 8 0.2614877852314663 1101 9 0.3248797107096024 1101 10-11 0.17520898696803044 1101 12-13 0.2744167779320108 1101 14-15 0.3016559937482839 1101 16-17 0.41809697664896817 1101 18-19 0.3910637962330341 1101 20-21 0.27451318572189365 1101 22-23 0.31739815072037914 1101 24-25 0.2706414053781856 1101 26-27 0.26565268532070263 1101 28-29 0.30140042053941585 1101 30-31 0.46114074895071866 1101 32-33 0.42923033011280864 1101 34-35 0.36277954148924607 1101 36-37 0.26124829217174295 1101 38-39 0.4304229244821798 1101 40-41 0.09238204764991309 1101 42-43 0.2163857702952754 1101 44-45 0.17016452292094186 1101 46-47 0.3520974725853492 1101 48-49 0.23520948371766792 1101 50-51 0.13605265453922755 1101 52-53 0.3981978498517975 1101 54-55 0.34483190663196694 1101 56-57 0.43510912473513486 1101 58-59 0.36454758903034445 1101 60-61 0.32625924337028067 1101 62-63 0.27664827289704164 1101 64-65 0.30456691199822217 1101 66-67 0.3508406723004214 1101 68-69 0.22798110526816728 1101 70-71 0.19715198017365054 1101 72-73 0.22475746498651006 1101 74-75 0.21964598369971355 1101 76-77 0.24524883353772609 1101 78-79 0.3903729682820547 1101 80-81 0.5953632341724493 1101 82-83 0.5443061575186512 1101 84-85 0.6900030377019313 1101 86-87 0.609168683657213 1101 88-89 0.7046316265815946 1101 90-91 0.5712062188055818 1101 92-93 0.6448026818531041 1101 94-95 0.7098279351017389 1101 96-97 0.9348330254600938 1101 98-99 0.9465084874505152 1101 100-101 1.274041173397034 1102 1 0.234618440382512 1102 2 0.16780810266939028 1102 3 0.14515223036192282 1102 4 0.12740332413802946 1102 5 0.21182775607970683 1102 6 0.14015925486383907 1102 7 0.18320194787352762 1102 8 0.11929855003694456 1102 9 0.2347428977328505 1102 10-11 0.2293043873940377 1102 12-13 0.27170987447482275 1102 14-15 0.2898333454204405 1102 16-17 0.26568204100478 1102 18-19 0.3644385163262527 1102 20-21 0.33125802906511836 1102 22-23 0.20846320742995772 1102 24-25 0.21072371617940178 1102 26-27 0.3266444883755639 1102 28-29 0.31599539769174356 1102 30-31 0.31026871414213986 1102 32-33 0.4555819613094485 1102 34-35 0.41029314996564636 1102 36-37 0.4660820320905472 1102 38-39 0.44517264685105573 1102 40-41 0.5744321714460909 1102 42-43 0.5898548167006439 1102 44-45 0.47134081413323514 1102 46-47 0.44711444904586983 1102 48-49 0.252327035466287 1102 50-51 0.4654130561485701 1102 52-53 0.5773569688963818 1102 54-55 0.5867225745851457 1102 56-57 0.31094656943647436 1102 58-59 0.23911521268997404 1102 60-61 0.3058914731578213 1102 62-63 0.39687392783340414 1102 64-65 0.4629423830608701 1102 66-67 0.6105657264612461 1102 68-69 0.7286837168212621 1102 70-71 0.5958564441180165 1102 72-73 0.5663370495411151 1102 74-75 0.7587100482193669 1102 76-77 0.5262303050737067 1102 78-79 0.41988834267445085 1102 80-81 0.6530154989142112 1102 82-83 0.7986276452573655 1102 84-85 0.7488779034376982 1102 86-87 0.7992270999764592 1102 88-89 0.7402833315097723 1102 90-91 0.8984491141796127 1102 92-93 0.9907680416812461 1102 94-95 1.0772988976169842 1102 96-97 1.3711530931838816 1102 98-99 1.2913593403697377 1102 100-101 1.7774774274704725 1103 1 0.1347185531462216 1103 2 0.16885423847484304 1103 3 0.07949747707358767 1103 4 0.11561361689792449 1103 5 0.1858179005072671 1103 6 0.1284048242962399 1103 7 0.1251316791747641 1103 8 0.030451209229013898 1103 9 0.04219308767948604 1103 10-11 0.0751246490982922 1103 12-13 0.12908811217391047 1103 14-15 0.17364658341755757 1103 16-17 0.19348711427685572 1103 18-19 0.2310257902058197 1103 20-21 0.27961784625896513 1103 22-23 0.19661024546672223 1103 24-25 0.051651468337666984 1103 26-27 0.17830060650618407 1103 28-29 0.14410129582973497 1103 30-31 0.14581069155777726 1103 32-33 0.24872989342740226 1103 34-35 0.45014301731319506 1103 36-37 0.46760014544544504 1103 38-39 0.5459514098076355 1103 40-41 0.41524192921699665 1103 42-43 0.5521014784934479 1103 44-45 0.5916823166518697 1103 46-47 0.38198762940819364 1103 48-49 0.7039456873378427 1103 50-51 0.7815972636337989 1103 52-53 0.5362870152547927 1103 54-55 0.4475597508456488 1103 56-57 0.5847026713884915 1103 58-59 0.337504285193269 1103 60-61 0.5440303002449554 1103 62-63 0.4523373720774089 1103 64-65 0.4496406446507706 1103 66-67 0.8060672208540822 1103 68-69 0.9466327310815466 1103 70-71 0.6540580880109523 1103 72-73 0.6153965612876426 1103 74-75 0.7090167728046524 1103 76-77 0.5675520611707974 1103 78-79 0.6736474370614474 1103 80-81 0.716421668439299 1103 82-83 0.6900655102644855 1103 84-85 0.758307114712121 1103 86-87 0.7642873805111599 1103 88-89 0.7945084429049913 1103 90-91 0.6659837491816134 1103 92-93 0.6296200657715652 1103 94-95 0.7859707552257369 1103 96-97 0.7822546620226589 1103 98-99 1.0974732742904472 1103 100-101 1.2566745304041547 1104 1 0.16701121173253597 1104 2 0.07849435859590415 1104 3 0.17825773262475053 1104 4 0.1363840061941346 1104 5 0.12037569537926629 1104 6 0.09882259636142976 1104 7 0.11203540075933205 1104 8 0.0993797466022599 1104 9 0.022099326372419625 1104 10-11 0.08625831447570675 1104 12-13 0.1562773237455488 1104 14-15 0.11879465130502354 1104 16-17 0.08940657725890588 1104 18-19 0.016989939372244578 1104 20-21 0.11080700035642366 1104 22-23 0.13469747822805545 1104 24-25 0.044501809460051334 1104 26-27 0.16883957074287537 1104 28-29 0.34228145390989795 1104 30-31 0.18267712022903737 1104 32-33 0.08068711172874288 1104 34-35 0.37578708747872724 1104 36-37 0.13942381462399567 1104 38-39 0.29040547682515694 1104 40-41 0.4476501893033884 1104 42-43 0.5147510909998303 1104 44-45 0.2985404246275891 1104 46-47 0.5407215513295043 1104 48-49 0.6798999222061255 1104 50-51 0.6431380161015952 1104 52-53 0.7103600153161906 1104 54-55 0.7829843610334706 1104 56-57 0.786689188465509 1104 58-59 0.830622403801712 1104 60-61 0.561987719705975 1104 62-63 0.8051387817626008 1104 64-65 1.037728334593183 1104 66-67 0.8579749068516662 1104 68-69 0.7537878761320478 1104 70-71 0.79926598130751 1104 72-73 0.6473744180488907 1104 74-75 0.8840400563836219 1104 76-77 0.8018736893624094 1104 78-79 0.7233397541988147 1104 80-81 0.624545906742977 1104 82-83 0.521641308927169 1104 84-85 0.6228894275519892 1104 86-87 0.714103267161601 1104 88-89 0.6287966315666367 1104 90-91 0.7329098334518385 1104 92-93 0.5376443773548338 1104 94-95 0.4198706012210245 1104 96-97 0.7565859040791381 1104 98-99 1.0050057896615279 1104 100-101 1.4770253580978299 1105 1 0.16682129404563995 1105 2 0.06456088537198212 1105 3 0.09196562069374892 1105 4 0.10432497421848552 1105 5 0.20017354884618754 1105 6 0.19867761041997056 1105 7 0.20208880931859596 1105 8 0.09399720142732804 1105 9 0.27702444785025193 1105 10-11 0.20136232915032792 1105 12-13 0.15167297787301948 1105 14-15 0.17032487832614152 1105 16-17 0.17806339599868437 1105 18-19 0.2262236446663266 1105 20-21 0.09547925338236496 1105 22-23 0.19759265756130162 1105 24-25 0.19363351505984383 1105 26-27 0.2536378179415806 1105 28-29 0.1775533372911653 1105 30-31 0.16379883893777958 1105 32-33 0.27297182150209665 1105 34-35 0.22796627713238848 1105 36-37 0.27818184559447445 1105 38-39 0.3240695448620059 1105 40-41 0.38991752855660167 1105 42-43 0.5691648243646625 1105 44-45 0.6120180954029593 1105 46-47 0.6383994601968084 1105 48-49 0.7247552373536834 1105 50-51 0.799166976232776 1105 52-53 0.9861946109073187 1105 54-55 0.8564107077119942 1105 56-57 0.9823460952159238 1105 58-59 1.1387545865117872 1105 60-61 1.015365243642556 1105 62-63 0.8545729660198091 1105 64-65 0.963579374276712 1105 66-67 0.7984148673858442 1105 68-69 0.8061773648828954 1105 70-71 0.847979867996127 1105 72-73 1.0801366086799327 1105 74-75 1.1077237183671969 1105 76-77 1.024825473430628 1105 78-79 0.9921983489005761 1105 80-81 1.0640409109813795 1105 82-83 1.043877890779374 1105 84-85 1.046512408518531 1105 86-87 1.2437628512881922 1105 88-89 1.3213314768300464 1105 90-91 1.4929071468113904 1105 92-93 1.3085828023350317 1105 94-95 1.4125101328191612 1105 96-97 1.3730633478797145 1105 98-99 1.0938733707123767 1105 100-101 1.0282614443111981 1106 1 -0.2320492691131406 1106 2 -0.43638789356252516 1106 3 -0.4741369774008959 1106 4 -0.3782070958092021 1106 5 -0.301204130154602 1106 6 -0.28822794058664414 1106 7 -0.24571535231174835 1106 8 -0.2841042009459187 1106 9 -0.6296962862187101 1106 10-11 -0.5313159568090526 1106 12-13 -0.5665169338775442 1106 14-15 -0.661406578244474 1106 16-17 -0.7669724106583686 1106 18-19 -0.5516711058568049 1106 20-21 -0.418615962432348 1106 22-23 -0.439996534157018 1106 24-25 -0.4408818928501077 1106 26-27 -0.2857690002697666 1106 28-29 -0.47646077742010107 1106 30-31 -0.5394900447523199 1106 32-33 -0.8503466952834913 1106 34-35 -1.1865526399599275 1106 36-37 -0.9032688763873082 1106 38-39 -0.8016371654744816 1106 40-41 -0.9562730590364978 1106 42-43 -1.1792332988726244 1106 44-45 -1.0928661665640078 1106 46-47 -0.8926470559649218 1106 48-49 -0.683102731739524 1106 50-51 -0.5799221959900933 1106 52-53 -0.7473252289699914 1106 54-55 -0.7492969124125182 1106 56-57 -0.9803850884182808 1106 58-59 -0.8809065824358484 1106 60-61 -0.8569297616212239 1106 62-63 -0.9423948404812776 1106 64-65 -1.4264629958510469 1106 66-67 -1.6617169219072458 1106 68-69 -1.6475794097886087 1106 70-71 -1.394309678539532 1106 72-73 -1.7899359144221911 1106 74-75 -1.9787386359042571 1106 76-77 -1.5827244674331489 1106 78-79 -1.2549975544539471 1106 80-81 -1.3607846046734693 1106 82-83 -1.6310613483779015 1106 84-85 -1.7301021402915957 1106 86-87 -1.5683431780069874 1106 88-89 -1.4738589937903015 1106 90-91 -1.330314542534989 1106 92-93 -1.2988664119980307 1106 94-95 -1.3597959198705638 1106 96-97 -1.8917467013788105 1106 98-99 -1.7710913425094432 1106 100-101 -1.4295406378036688 1107 1 0.15793395227620977 1107 2 0.18802316681450293 1107 3 0.18087556759746803 1107 4 0.028741742414865712 1107 5 0.08790639569640746 1107 6 0.09064139922956826 1107 7 0.0560944520070592 1107 8 -0.0941604586404452 1107 9 0.06330708508727412 1107 10-11 0.2624993330245644 1107 12-13 0.043714122655465815 1107 14-15 0.05900748462077132 1107 16-17 0.13591943223566005 1107 18-19 0.3347615952917238 1107 20-21 0.15107241253583226 1107 22-23 0.2892691548828594 1107 24-25 -0.02636579733150768 1107 26-27 0.08593436636105167 1107 28-29 0.00984881132212223 1107 30-31 0.1976195149000617 1107 32-33 0.27559547824785824 1107 34-35 0.14182502942352215 1107 36-37 -0.029742199435297323 1107 38-39 -0.017969558582265677 1107 40-41 -0.1494179836366385 1107 42-43 -0.0349294553760231 1107 44-45 0.06198274802092385 1107 46-47 -0.09395532007999208 1107 48-49 -0.2750266617784334 1107 50-51 -0.3676286170553027 1107 52-53 -0.3917290688303652 1107 54-55 -0.2350831435625551 1107 56-57 -0.04292567135812675 1107 58-59 -0.1979405084748933 1107 60-61 -0.03482589007369086 1107 62-63 -0.11703524316792624 1107 64-65 -0.08218850193653537 1107 66-67 -0.10574752858648395 1107 68-69 -0.10243299952820095 1107 70-71 -0.23089743898460213 1107 72-73 0.13911731481103473 1107 74-75 0.1987588057472145 1107 76-77 0.1119723487865194 1107 78-79 0.2478490952782728 1107 80-81 0.41860523569597063 1107 82-83 0.14971805185169984 1107 84-85 0.15318522068748663 1107 86-87 0.18357883002389386 1107 88-89 0.3566315857730409 1107 90-91 0.2661520794579779 1107 92-93 0.31810725649194893 1107 94-95 0.266077424994819 1107 96-97 0.35947663075976166 1107 98-99 0.25056111688164506 1107 100-101 0.7983562698412463 1108 1 0.07611453296125603 1108 2 0.08819842729467808 1108 3 0.1022635852256073 1108 4 0.12373122111419121 1108 5 -0.012732255762117006 1108 6 -0.10937675072307229 1108 7 0.03575799725999218 1108 8 -0.05244959872972288 1108 9 0.03407148168642493 1108 10-11 0.02117869709559983 1108 12-13 0.18558719776790866 1108 14-15 -0.010535193291310918 1108 16-17 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70-71 -0.2167703609899938 2316 72-73 -0.09218425371702921 2316 74-75 -0.34706033010740356 2316 76-77 -0.06560642962017127 2316 78-79 -0.19357985162081093 2316 80-81 -0.4265882724188863 2316 82-83 -0.32197147158235495 2316 84-85 -0.2847776640524522 2316 86-87 -0.1344165329152922 2316 88-89 0.10643681044521003 2316 90-91 -0.2617430328546426 2316 92-93 0.22137431928000595 2316 94-95 0.378408815268255 2316 96-97 0.062434869052122366 2316 98-99 -0.4290428555963679 2316 100-101 0.33980586761846254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 6.0 5 19.0 6 56.0 7 105.0 8 231.0 9 447.0 10 797.0 11 1297.0 12 2002.0 13 2985.0 14 3771.0 15 5339.0 16 7455.0 17 10992.0 18 10172.0 19 5414.0 20 4258.0 21 4213.0 22 4335.0 23 4431.0 24 4741.0 25 5331.0 26 6403.0 27 7593.0 28 9293.0 29 11355.0 30 14190.0 31 17877.0 32 22830.0 33 29988.0 34 42445.0 35 62830.0 36 95719.0 37 142976.0 38 111642.0 39 9515.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.39748259514203 20.219209169123616 14.208007290877653 20.1753009448567 2 16.46150551386308 22.320739123090657 39.0820684829034 22.135686880142856 3 20.349412417654015 27.206299317101422 29.861881952655583 22.582406312588983 4 12.758047585724283 18.021403923650492 36.50611713037813 32.7144313602471 5 12.763306737454942 39.68265938673625 32.54008235169907 15.013951524109743 6 30.2336454236143 36.56523732535412 16.994039719118746 16.207077531912837 7 27.11339615356772 35.14725754687387 19.928784295745757 17.81056200381265 8 27.90684346420212 33.363999420863394 19.168064235901642 19.56109287903284 9 28.073797688279722 16.02866122921744 20.293459375980312 35.60408170652253 10-11 25.561415627036027 27.50815919017398 25.60741475833112 21.323010424458868 12-13 26.10517028143947 25.668984344363672 27.564025217942145 20.661820156254713 14-15 23.0553678723969 26.098172703361406 27.3939908544678 23.452468569773895 16-17 20.651423107550492 29.76837552182621 27.851267464588208 21.728933906035085 18-19 20.54155305132598 26.705512053280568 32.53895598561811 20.213978909775342 20-21 21.5021054028619 25.59587727130137 33.07329999276079 19.82871733307594 22-23 21.10986350107195 26.024307532439327 32.90415212832302 19.961676838165708 24-25 20.768683188147005 26.101339856663692 33.11507625298618 20.014900702203132 26-27 20.333625166748487 26.4376189108053 33.06532920996095 20.163426712485265 28-29 20.277249703453066 26.642244119396995 33.0834773021883 19.997028874961636 30-31 20.352881204604138 26.33774522815569 33.051808595352426 20.257564971887746 32-33 20.26691561497068 26.46141502376873 32.86871697111556 20.40295239014503 34-35 20.697723872493423 26.551829709707775 32.88191344320842 19.86853297459038 36-37 21.001667283760234 26.307415152148128 32.60591700983856 20.08500055425308 38-39 20.638302043869597 26.62278902536136 32.59724668806255 20.1416622427065 40-41 20.655661554726564 26.746780623944755 32.57336678453011 20.024191036798577 42-43 20.56590752242926 26.95584292163052 32.05768440265946 20.420565153280762 44-45 20.62269250259405 27.204414106802442 31.79030127168746 20.382592118916047 46-47 21.00086568856929 27.54314869332304 31.029354986607466 20.426630631500206 48-49 21.046261552568712 27.829776067179846 30.402786491638718 20.721175888612727 50-51 21.280480080113897 28.23735853381916 30.136821022658722 20.34534036340822 52-53 21.399700779421345 28.461623150985737 29.56658261142347 20.572093458169448 54-55 20.87915651166719 28.65338674259791 29.616276754904565 20.85117999083034 56-57 21.102365411066334 28.867999083033713 29.414106802442024 20.615528703457926 58-59 20.983974204290437 28.26239412658961 29.715061774571076 21.03856989454888 60-61 20.824711638232667 28.537484013416663 30.09361200260611 20.544192345744552 62-63 20.839491686976665 28.415548611278684 30.3331845274004 20.41177517434425 64-65 20.657908230978983 28.517349967423566 30.137348881542437 20.687392920055018 66-67 20.658813131922493 28.639059144325667 29.907353225066963 20.794774498684877 68-69 20.85917328249801 28.49910113172945 29.85479356193142 20.786932023841125 70-71 20.823128061581524 28.744178470596754 29.47217127965059 20.960522188171137 72-73 20.83428850655148 28.82629823122029 29.160508313023335 21.178904949204895 74-75 20.66808836659347 29.300466325619556 28.944538621172267 21.086906686614707 76-77 20.808725054897323 28.857517313771385 29.1688786467508 21.164878984580486 78-79 21.025976689751698 28.96874472141116 28.85140923240269 21.15386935643445 80-81 20.963463116237545 29.30484001351319 28.74553582201202 20.986161048237253 82-83 20.7780651680479 29.175935631282474 28.78622437052733 21.259774830142295 84-85 20.67979482786444 29.101821423023498 28.66829650118467 21.55008724792739 86-87 20.57888021524577 29.00448830867981 28.786859631765644 21.629771844308777 88-89 20.57609010400328 28.84371757438286 28.783240027991603 21.796952293622258 90-91 20.439527289456024 28.990173231671907 28.73081593097883 21.83948354789324 92-93 20.293498834903133 28.823742336377418 28.962271976592486 21.92048685212696 94-95 20.023949711638235 28.901932868415336 29.00825872927777 22.065858690668662 96-97 20.289311913322557 28.89869030670109 28.72683453584614 22.08516324413021 98-99 19.912812399612097 29.117129399912223 28.44041124917616 22.52964695129952 100-101 19.739248546499567 29.106963603851465 28.73522648511958 22.418561364529385 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1110.0 1 760.5 2 490.0 3 580.0 4 521.0 5 534.5 6 965.5 7 1839.5 8 2700.5 9 3113.5 10 2823.5 11 2376.0 12 2351.5 13 2444.0 14 2436.5 15 2474.0 16 2510.5 17 2498.5 18 2576.0 19 2593.5 20 2711.0 21 3012.5 22 3330.5 23 3830.5 24 4427.5 25 5135.5 26 6018.5 27 6918.0 28 7861.0 29 8812.0 30 9941.5 31 10844.0 32 11937.0 33 12791.5 34 13537.0 35 14487.0 36 15721.5 37 17192.5 38 17909.5 39 18021.0 40 18170.5 41 19094.0 42 19872.5 43 20779.5 44 22170.0 45 23096.0 46 22956.0 47 22817.0 48 23774.0 49 23299.5 50 22072.5 51 21013.5 52 19582.0 53 19301.0 54 19362.5 55 18120.5 56 15836.0 57 14165.5 58 13324.0 59 12048.5 60 9887.5 61 8073.0 62 6597.0 63 4700.0 64 3141.0 65 2260.0 66 1659.5 67 1243.5 68 886.5 69 603.0 70 419.5 71 269.5 72 206.0 73 188.0 74 148.0 75 111.0 76 82.5 77 48.5 78 30.5 79 22.5 80 14.0 81 9.0 82 8.5 83 4.0 84 1.0 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04675321541468594 2 0.0 3 0.0 4 0.0 5 0.00693757390024372 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005429405661060303 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015835766511425881 24-25 0.0 26-27 9.803093554692213E-4 28-29 0.001131126179387563 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.262252358775126E-4 38-39 0.0 40-41 7.540841195917087E-5 42-43 0.02088813011269033 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5081682391834174E-4 84-85 4.524504717550252E-4 86-87 0.0 88-89 0.0 90-91 0.010707994498202263 92-93 0.0031671533022851763 94-95 0.0 96-97 0.0 98-99 4.524504717550252E-4 100-101 0.013196472092854902 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 663056.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.32407454048519 #Duplication Level Percentage of deduplicated Percentage of total 1 78.51812914021552 45.009790876131575 2 12.032588735250677 13.795140271490244 3 3.9849720437352736 6.853045034304915 4 1.7619935039204992 4.040185878343576 5 0.975210632925698 2.795152350725323 6 0.5901106946605699 2.0296529668716 7 0.3959602643647907 1.5888638996662239 8 0.2782822765554009 1.2761819171646174 9 0.21128651492396716 1.0900623537810747 >10 1.1528403114944088 11.897864775822487 >50 0.06742523670520982 2.6732766750819708 >100 0.027763286025802334 2.913362991302539 >500 0.0018508857350410407 0.6864690176139443 >1k 0.0010576489914520231 1.30579169501968 >5k 5.288244957260116E-4 2.0451592966802714 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7196 1.0852778649163872 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6297 0.9496935402137979 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4341 0.6546958326295215 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1484 0.22381216669481915 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1476 0.22260563210347242 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1314 0.19817330662870106 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 870 0.1312106368089573 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 664 0.10014237108177891 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.5081682391834174E-4 0.0 0.0 0.0 0.0 8 1.5081682391834174E-4 0.0 0.0 0.0 0.0 9 1.5081682391834174E-4 0.0 0.0 0.0 0.0 10-11 1.5081682391834174E-4 0.0 0.0 1.5081682391834174E-4 0.0 12-13 1.5081682391834174E-4 0.0 0.0 1.5081682391834174E-4 0.0 14-15 1.5081682391834174E-4 0.0 0.0 1.5081682391834174E-4 0.0 16-17 2.262252358775126E-4 0.0 0.0 1.5081682391834174E-4 0.0 18-19 3.016336478366835E-4 0.0 0.0 1.5081682391834174E-4 0.0 20-21 3.016336478366835E-4 0.0 0.0 2.262252358775126E-4 0.0 22-23 3.016336478366835E-4 0.0 0.0 6.786757076325379E-4 0.0 24-25 3.016336478366835E-4 0.0 0.0 0.001206534591346734 0.0 26-27 3.016336478366835E-4 0.0 0.0 0.0017343934750609301 0.0 28-29 4.524504717550252E-4 0.0 0.0 0.004222871069713569 0.0 30-31 4.524504717550252E-4 0.0 0.0 0.010255544026447239 0.0 32-33 4.524504717550252E-4 0.0 0.0 0.014704640332038318 0.0 34-35 4.524504717550252E-4 0.0 0.0 0.019757003933302767 0.0 36-37 6.03267295673367E-4 0.0 0.0 0.027599478777056537 0.0 38-39 7.540841195917087E-4 0.0 0.0 0.043359836876523256 0.0 40-41 7.540841195917087E-4 0.0 0.0 0.06010050433145918 0.0 42-43 7.540841195917087E-4 0.0 0.0 0.08249680268333293 0.0 44-45 7.540841195917087E-4 0.0 0.0 0.10240462344055404 0.0 46-47 7.540841195917087E-4 0.0 0.0 0.13430238169928332 0.0 48-49 7.540841195917087E-4 0.0 0.0 0.17879334475519412 0.0 50-51 7.540841195917087E-4 0.0 0.0 0.20081260104727203 0.0 52-53 8.294925315508795E-4 0.0 0.0 0.23844139861489827 0.0 54-55 9.049009435100504E-4 0.0 0.0 0.30653519461402956 0.0 56-57 0.0010557177674283922 0.0 0.0 0.40961849376221615 0.0 58-59 0.0010557177674283922 0.0 0.0 0.5437700586375811 0.0 60-61 0.0010557177674283922 0.0 0.0 0.6887804348350667 0.0 62-63 0.0010557177674283922 0.0 0.0 0.8369579643348375 0.0 64-65 0.0010557177674283922 0.0 0.0 0.986342028425955 0.0 66-67 0.001206534591346734 0.0 0.0 1.1759941845032698 0.0 68-69 0.0013573514152650757 0.0 0.0 1.3998063511980887 0.0 70-71 0.001658985063101759 0.0 0.0 1.5403676310899832 0.0 72-73 0.001658985063101759 0.0 0.0 1.7331869404695832 0.0 74-75 0.001658985063101759 0.0 0.0 1.9472714220216694 0.0 76-77 0.00173439347506093 0.0 0.0 2.1833751598658333 0.0 78-79 0.0018098018870201008 0.0 0.0 2.4486619531381963 0.0 80-81 0.0018098018870201008 0.0 0.0 2.816127144615236 0.0 82-83 0.0019606187109384426 0.0 0.0 3.2472370357858162 0.0 84-85 0.0020360271228976132 0.0 0.0 3.768535387659564 0.0 86-87 0.0021114355348567843 0.0 0.0 4.3905039694988055 0.0 88-89 0.0021114355348567843 0.0 0.0 5.130939166525904 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3740 0.0 47.771458 1 GTATCAA 5865 0.0 33.94675 1 TATCAAC 7395 0.0 26.718424 2 ATCAACG 7485 0.0 26.397161 3 GGCTTAC 180 6.682967E-9 26.390566 7 TCAACGC 7555 0.0 26.215446 4 CAACGCA 7570 0.0 26.163502 5 GGACCGA 220 7.6397555E-11 25.90683 6 AACGCAG 7725 0.0 25.638538 6 ATGGGAG 1155 0.0 25.49561 5 TCGCACG 85 1.12322596E-7 25.14676 22-23 TACATGG 4900 0.0 24.620298 2 GTACATG 4900 0.0 24.53447 1 GTGTACA 610 0.0 24.148163 1 ACATGGG 4955 0.0 23.484327 3 GAGTACT 5500 0.0 22.931002 12-13 ACGCAGA 8730 0.0 22.581617 7 CGCAGAG 8735 0.0 22.459927 8 CTTACTA 255 4.8930815E-10 22.35436 9 GTACTTT 5650 0.0 22.234783 14-15 >>END_MODULE