FastQCFastQC Report
Thu 26 May 2016
SRR937768_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937768_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488896
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65261.3348442204477027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48780.9977582144259719No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33480.6848082209713313No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11390.23297388401623248No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.23133754418117555No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6680.13663437622725488No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6470.1323389841602304No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.12967993192826288No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.1268163372169132No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5970.1221118601911245No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.10206669721167692No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.052.1758421
GTCGGGA707.881681E-847.5205082
GTATCAA40450.044.529351
CGGGAGT905.660677E-736.9679834
ATCAACG50700.034.960853
TATCAAC51050.034.8142432
TCAACGC51850.034.100794
CAACGCA52500.033.588055
GGTCGGG1001.2917553E-633.267771
CCATATA1001.2927903E-633.264362
AACGCAG53800.032.854686
GTACATG31200.029.0940671
CGCAGAG60800.029.072078
ACGCAGA60950.029.0005237
TACATGG31050.028.3133492
GCAGAGT62650.027.531019
ACATGGG31900.026.963053
TAGGCCA1501.052158E-625.3494744
ATGGGAG8850.025.242065
CATGGGA23050.024.7446044
Produced by FastQC (version 0.11.5)