##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937768_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 488896 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95124116376489 33.0 31.0 34.0 30.0 34.0 2 32.0158581784265 33.0 31.0 34.0 30.0 34.0 3 32.02525281450452 34.0 31.0 34.0 30.0 34.0 4 35.639680013745256 37.0 35.0 37.0 35.0 37.0 5 35.66695779879566 37.0 35.0 37.0 35.0 37.0 6 35.75300268359733 37.0 35.0 37.0 35.0 37.0 7 35.68820567155387 37.0 35.0 37.0 35.0 37.0 8 35.69591692302657 37.0 35.0 37.0 35.0 37.0 9 37.25999190011782 39.0 37.0 39.0 34.0 39.0 10-11 37.21539857147532 39.0 37.0 39.0 34.0 39.0 12-13 37.12560544573897 39.0 37.0 39.0 34.0 39.0 14-15 38.23293399823275 40.0 38.0 41.0 33.5 41.0 16-17 38.17534915401231 40.0 38.0 41.0 33.5 41.0 18-19 38.19096392688833 40.0 38.0 41.0 33.5 41.0 20-21 38.23651144619714 40.0 38.0 41.0 34.0 41.0 22-23 38.20998330933368 40.0 38.0 41.0 34.0 41.0 24-25 38.13369816893572 40.0 38.0 41.0 34.0 41.0 26-27 37.999773980560285 40.0 38.0 41.0 33.5 41.0 28-29 37.840674703822486 40.0 38.0 41.0 33.0 41.0 30-31 37.510052240149236 40.0 37.0 41.0 33.0 41.0 32-33 37.2678105363922 39.5 36.0 41.0 33.0 41.0 34-35 37.19328446131693 40.0 36.0 41.0 33.0 41.0 36-37 37.02028959124231 40.0 36.0 41.0 32.0 41.0 38-39 36.82667888467077 40.0 35.0 41.0 31.0 41.0 40-41 36.537930357376624 39.0 35.0 41.0 30.5 41.0 42-43 36.18611422470218 39.0 35.0 41.0 30.0 41.0 44-45 35.7763174581097 39.0 35.0 41.0 29.0 41.0 46-47 35.18089123249116 39.0 35.0 41.0 25.0 41.0 48-49 34.65309493225553 39.0 35.0 40.0 22.5 41.0 50-51 33.693788658528604 38.0 34.0 39.5 20.0 40.5 52-53 33.76290049417463 38.0 33.5 39.5 20.0 40.5 54-55 34.012623339115066 38.0 34.0 40.0 18.5 41.0 56-57 33.77789754876292 38.0 34.0 40.0 15.0 41.0 58-59 33.508599988545626 38.0 34.0 40.0 11.5 41.0 60-61 33.174498257298076 37.5 33.0 40.0 8.0 41.0 62-63 32.813154535934025 37.0 33.0 40.0 6.5 41.0 64-65 32.38211603285771 36.5 32.5 39.5 2.0 41.0 66-67 31.85487915630318 36.0 31.5 39.0 2.0 40.5 68-69 31.426914926691975 35.0 31.0 39.0 2.0 40.0 70-71 30.97402003698128 35.0 31.0 38.0 2.0 40.0 72-73 30.52187786359471 35.0 30.5 37.5 2.0 40.0 74-75 30.010406098638565 35.0 30.0 37.0 2.0 39.0 76-77 29.51640635227124 34.0 29.0 36.0 2.0 39.0 78-79 29.050200860714753 34.0 29.0 36.0 2.0 38.5 80-81 28.55706121547323 34.0 28.5 35.5 2.0 37.0 82-83 28.068689455426103 34.0 27.0 35.0 2.0 37.0 84-85 27.583436968189552 34.0 26.0 35.0 2.0 36.5 86-87 27.063335556028278 33.0 25.5 35.0 2.0 36.0 88-89 26.56389804784658 33.0 25.0 35.0 2.0 35.5 90-91 26.04940314504516 33.0 23.5 35.0 2.0 35.0 92-93 25.47350049908365 32.0 20.5 35.0 2.0 35.0 94-95 24.799163830344284 32.0 17.0 34.0 2.0 35.0 96-97 24.014807852794867 31.5 4.5 34.0 2.0 35.0 98-99 22.952435691844485 31.0 2.0 34.0 2.0 35.0 100-101 19.887100733080246 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09522358774842843 1101 2 -0.13155742856437413 1101 3 -0.10352883966315218 1101 4 -0.03312974439911898 1101 5 -0.004877397720896681 1101 6 -0.05068724354730847 1101 7 -0.07724839581901932 1101 8 -0.16220245111257015 1101 9 0.0702963546515818 1101 10-11 0.1156859183068022 1101 12-13 0.05722022382656178 1101 14-15 0.30720092295456425 1101 16-17 0.1745357524222797 1101 18-19 0.24965611692285705 1101 20-21 0.011477234969497374 1101 22-23 0.04625149554464514 1101 24-25 -8.437692764857729E-4 1101 26-27 0.26211206933735554 1101 28-29 0.24852162458530813 1101 30-31 0.3473806237638897 1101 32-33 0.31161352182894575 1101 34-35 0.48359302298381834 1101 36-37 0.6173891919954642 1101 38-39 0.5174566650078489 1101 40-41 0.6066149571680981 1101 42-43 0.6402228935328509 1101 44-45 1.0111915157419418 1101 46-47 0.9762504168736896 1101 48-49 1.109622539636348 1101 50-51 0.9446928437694169 1101 52-53 1.0885113654003646 1101 54-55 1.1471826998600676 1101 56-57 1.34873779428775 1101 58-59 1.313670967792568 1101 60-61 1.3815863826003252 1101 62-63 1.5089367864146084 1101 64-65 1.5098434398317018 1101 66-67 1.5540371775184276 1101 68-69 1.450558240331116 1101 70-71 1.504908744729775 1101 72-73 1.5474141112803963 1101 74-75 1.8656200940166876 1101 76-77 1.577368612219086 1101 78-79 1.620417762481413 1101 80-81 1.6996316344228966 1101 82-83 1.7179016221862113 1101 84-85 1.8575043851309374 1101 86-87 2.0227486438068247 1101 88-89 2.121103744120518 1101 90-91 2.0991349982488465 1101 92-93 2.1366356554770896 1101 94-95 2.2752461129657497 1101 96-97 2.4273628515228403 1101 98-99 2.8021802527879096 1101 100-101 3.8116593494391893 1102 1 0.037203773042385535 1102 2 0.07902276958268928 1102 3 0.03963980952030255 1102 4 0.184978487716009 1102 5 0.06540069266632287 1102 6 0.03623860748520258 1102 7 -0.03594808002121397 1102 8 -0.08365353682634691 1102 9 0.08735362121452539 1102 10-11 0.09224245179436963 1102 12-13 0.010405776338217265 1102 14-15 0.1429143226159013 1102 16-17 0.19496172824947422 1102 18-19 -0.11685840484447141 1102 20-21 0.1836027350823386 1102 22-23 -0.04432030324746705 1102 24-25 0.053528603122813934 1102 26-27 0.03669475441086689 1102 28-29 0.05153806908754177 1102 30-31 0.3538206274105846 1102 32-33 0.30379479844123125 1102 34-35 0.37558583720115024 1102 36-37 0.46603490810026216 1102 38-39 0.6167917409831531 1102 40-41 0.6083695555160276 1102 42-43 0.80642625058465 1102 44-45 0.7302749398690125 1102 46-47 1.108965456053248 1102 48-49 1.192544498868088 1102 50-51 0.943283485811925 1102 52-53 0.930856818016494 1102 54-55 0.9360540029108932 1102 56-57 0.8821424175198871 1102 58-59 0.9214621915128234 1102 60-61 0.842320145466509 1102 62-63 1.029900051940345 1102 64-65 1.219828060941584 1102 66-67 1.079074578560725 1102 68-69 0.917473450187952 1102 70-71 0.8043136160567386 1102 72-73 0.7105521521383267 1102 74-75 0.611379690130267 1102 76-77 0.7195120020612471 1102 78-79 0.7513243996158288 1102 80-81 0.9919366595387586 1102 82-83 1.2016753238357403 1102 84-85 1.374675984550457 1102 86-87 1.198839177494662 1102 88-89 1.1338619458216108 1102 90-91 1.0974267308624057 1102 92-93 1.0077055569415982 1102 94-95 1.4008187113405768 1102 96-97 1.3884717457223559 1102 98-99 1.6914245948060547 1102 100-101 2.5085061538732134 1103 1 0.2088788186519004 1103 2 -0.07790203456468348 1103 3 0.16290025017839938 1103 4 0.05614749470809244 1103 5 0.06473533030800382 1103 6 0.033918919858791696 1103 7 0.1261097553222399 1103 8 0.12224061607491876 1103 9 0.18890971060092454 1103 10-11 0.19747867194180202 1103 12-13 0.17766433482984212 1103 14-15 0.3006142821658315 1103 16-17 0.3186019042485526 1103 18-19 0.15299143459350972 1103 20-21 0.11029152546017684 1103 22-23 0.11141658969193458 1103 24-25 0.00989040835160182 1103 26-27 -0.005755529152374095 1103 28-29 0.028049787224858846 1103 30-31 -0.018473551907042918 1103 32-33 0.14626902184118507 1103 34-35 0.3031678311418915 1103 36-37 0.22316361361549752 1103 38-39 0.3737297533162902 1103 40-41 0.3270533598037346 1103 42-43 0.4349369450875713 1103 44-45 0.6275614832042535 1103 46-47 0.42175263090485515 1103 48-49 0.495584032619135 1103 50-51 0.6418239812839914 1103 52-53 0.7108596247559689 1103 54-55 0.915301466526472 1103 56-57 0.9369687711525501 1103 58-59 0.859640452490062 1103 60-61 0.8712468978212868 1103 62-63 0.9324756481661041 1103 64-65 0.9075568308467332 1103 66-67 0.8376239895581854 1103 68-69 1.1229020145407098 1103 70-71 1.0649705581966273 1103 72-73 1.1034189063092263 1103 74-75 1.2185864064927365 1103 76-77 1.2352049777829492 1103 78-79 1.3673054788897083 1103 80-81 1.268790139482956 1103 82-83 1.198173503582641 1103 84-85 1.3020573791697707 1103 86-87 1.2287359059921528 1103 88-89 1.3761438250162392 1103 90-91 1.373587606565124 1103 92-93 1.420666089231485 1103 94-95 1.7155227241525175 1103 96-97 1.4757869343104808 1103 98-99 1.5848031661756607 1103 100-101 2.13351750465786 1104 1 0.022740359925762732 1104 2 0.05216351266327379 1104 3 0.12560187536664458 1104 4 0.1181988705034982 1104 5 -0.01277927201270046 1104 6 0.03710488073937057 1104 7 0.1406520128279638 1104 8 0.12738690042627354 1104 9 0.15578442225405809 1104 10-11 -0.13101913879818738 1104 12-13 -0.071200993036868 1104 14-15 -0.013081867696193683 1104 16-17 0.12466915761948627 1104 18-19 0.004290627657518087 1104 20-21 -0.09348026921073682 1104 22-23 0.003059100507663004 1104 24-25 0.0557900070598123 1104 26-27 0.034000213846788085 1104 28-29 0.07255835711143277 1104 30-31 0.20487188111378174 1104 32-33 0.15927313921686448 1104 34-35 0.36801432179728266 1104 36-37 0.4125054428445125 1104 38-39 0.7313982566981139 1104 40-41 0.7639661114815866 1104 42-43 0.79721551645396 1104 44-45 0.9168946076221189 1104 46-47 0.8261415966862486 1104 48-49 1.0938778896339016 1104 50-51 0.9605706625747388 1104 52-53 0.8292372220539974 1104 54-55 0.9381455024251082 1104 56-57 1.1280132614423337 1104 58-59 1.0309189281035387 1104 60-61 0.988198647053089 1104 62-63 1.184714090353097 1104 64-65 1.3237617072819425 1104 66-67 1.2522837620144252 1104 68-69 1.2922323534190063 1104 70-71 1.2139412142782433 1104 72-73 0.9241746580131931 1104 74-75 1.1606567587764438 1104 76-77 1.2566492800488085 1104 78-79 1.2275784617673473 1104 80-81 1.3239490458104832 1104 82-83 1.462650910245305 1104 84-85 1.8721537414291944 1104 86-87 1.6636569968790837 1104 88-89 1.8076622052249824 1104 90-91 1.7048135051192546 1104 92-93 1.6706630425475808 1104 94-95 1.6678151361686382 1104 96-97 2.2560061214552185 1104 98-99 2.499805908191174 1104 100-101 3.261321541949272 1105 1 -0.06708696291828886 1105 2 0.017577793258933383 1105 3 -0.049763604707703024 1105 4 -0.03705760986628803 1105 5 -0.08859923182403406 1105 6 0.01891311920608274 1105 7 0.003161939511940659 1105 8 -0.07310801941640221 1105 9 0.18257127775297022 1105 10-11 0.03811793639734873 1105 12-13 0.04247551545240924 1105 14-15 0.1448000765299824 1105 16-17 0.22812039586199973 1105 18-19 -0.05505575127674689 1105 20-21 0.03334451198314525 1105 22-23 -0.05625480062150956 1105 24-25 -0.09555027861915022 1105 26-27 -0.0034634581567232203 1105 28-29 0.2181312132096167 1105 30-31 0.34594079901617647 1105 32-33 0.4215083665300625 1105 34-35 0.18743667784202245 1105 36-37 0.31296097025263947 1105 38-39 0.39143421277135815 1105 40-41 0.4909451098660824 1105 42-43 0.4921064434124247 1105 44-45 0.8314538838070789 1105 46-47 1.0053384100897347 1105 48-49 1.1667748469874084 1105 50-51 1.0182397200269335 1105 52-53 0.8937905265135129 1105 54-55 0.9657422077089279 1105 56-57 0.8175945911046298 1105 58-59 0.7819084534875671 1105 60-61 0.9190041594795133 1105 62-63 0.8627008435993133 1105 64-65 0.9407593332591553 1105 66-67 1.0382864566245793 1105 68-69 0.8760544253353437 1105 70-71 1.2948759045552265 1105 72-73 1.281302698092901 1105 74-75 1.6143633144737812 1105 76-77 1.2422741844488208 1105 78-79 1.4419246416440963 1105 80-81 1.5205319616468707 1105 82-83 1.536130372748108 1105 84-85 1.6865745154905305 1105 86-87 1.8254897089434365 1105 88-89 1.6726341590687852 1105 90-91 1.7099072821622627 1105 92-93 1.7929660751364374 1105 94-95 2.269182556529916 1105 96-97 1.871762257141203 1105 98-99 1.9100885908159668 1105 100-101 2.373442258807767 1106 1 0.002344413459777428 1106 2 -0.08634292253577058 1106 3 -0.1327004578384141 1106 4 0.17093472090471806 1106 5 0.13151385379793368 1106 6 0.03498832025705667 1106 7 0.04635461113756634 1106 8 0.20891367456005128 1106 9 -0.14777809823453225 1106 10-11 -0.3184701443425837 1106 12-13 -0.3065454151517173 1106 14-15 -0.6266830848966336 1106 16-17 -0.0036784999110466288 1106 18-19 0.1775065168267176 1106 20-21 -0.05320504508338075 1106 22-23 0.04655192415613385 1106 24-25 -0.09604292006493864 1106 26-27 -0.3570646227433443 1106 28-29 -0.13973729146825775 1106 30-31 -0.28415870567945234 1106 32-33 -0.22222976125491556 1106 34-35 0.09424755678537622 1106 36-37 -0.32008994490652043 1106 38-39 -0.40189160519096845 1106 40-41 -0.17834158082610685 1106 42-43 -0.15040673807490634 1106 44-45 -0.37776339326156716 1106 46-47 -0.2843613893031218 1106 48-49 -0.18165802062962655 1106 50-51 -0.36902305758317766 1106 52-53 -0.08510799822507664 1106 54-55 0.17513257501529722 1106 56-57 -0.002222909649866267 1106 58-59 -0.2426126635214345 1106 60-61 0.09068970333791526 1106 62-63 0.12934857389809196 1106 64-65 0.5161157216203911 1106 66-67 0.6119030661862546 1106 68-69 0.363978052596611 1106 70-71 0.4866443541992851 1106 72-73 0.043632048871359075 1106 74-75 0.18481547943321885 1106 76-77 0.5331989301393172 1106 78-79 0.5700158557611594 1106 80-81 0.7945737518437461 1106 82-83 1.0958908868704924 1106 84-85 0.6320577479285276 1106 86-87 0.25906078245796493 1106 88-89 0.6833124952721583 1106 90-91 0.7534017521510812 1106 92-93 0.19667464443466898 1106 94-95 0.5535565024547502 1106 96-97 0.7262976651903408 1106 98-99 0.620626235723563 1106 100-101 1.8162559071949218 1107 1 -0.16023738676247135 1107 2 0.07074437219197094 1107 3 -0.044263613783595446 1107 4 -0.05409306030284 1107 5 -0.11286816867397675 1107 6 0.15864556189182366 1107 7 0.12627188487506658 1107 8 -0.08924041853786235 1107 9 -0.14342570905068186 1107 10-11 0.09270716149764269 1107 12-13 0.011549411370076257 1107 14-15 -0.1545568964783115 1107 16-17 -0.3810399025533471 1107 18-19 0.04756763548333964 1107 20-21 -0.08541581184470459 1107 22-23 -0.1500927233312126 1107 24-25 -0.0965985442160715 1107 26-27 0.06803415488352016 1107 28-29 0.14165547307057125 1107 30-31 -0.10231526821803527 1107 32-33 0.022275733250580743 1107 34-35 0.09182441812628639 1107 36-37 0.020245281664664105 1107 38-39 0.06932397697966763 1107 40-41 0.36930319013327306 1107 42-43 0.41103603263752575 1107 44-45 0.3322934038738836 1107 46-47 -0.0061788976374757 1107 48-49 -0.08389903803919196 1107 50-51 -0.24578253406176032 1107 52-53 -0.1329379772348318 1107 54-55 0.07823789665994241 1107 56-57 0.34839136371721224 1107 58-59 0.053334366976152126 1107 60-61 0.2730887649504652 1107 62-63 0.017544647487426346 1107 64-65 0.002981384047853197 1107 66-67 0.12804703457741695 1107 68-69 0.4861381958241715 1107 70-71 0.6276185485442625 1107 72-73 0.2550779063846349 1107 74-75 0.14098290751075737 1107 76-77 0.0059424787255615286 1107 78-79 0.10523625334110775 1107 80-81 0.47090724980646215 1107 82-83 0.3941221499893963 1107 84-85 0.5342061613128948 1107 86-87 0.7259702774573498 1107 88-89 0.538356327844081 1107 90-91 1.0298710693507331 1107 92-93 1.3703380596711163 1107 94-95 1.3740590336314433 1107 96-97 1.3717940227653465 1107 98-99 1.256615246712574 1107 100-101 1.8529579691778828 1108 1 0.24028330383768903 1108 2 0.33180904744546424 1108 3 0.3586892500147094 1108 4 0.22125635049464165 1108 5 0.24736751233452026 1108 6 0.3310215553146776 1108 7 0.3010115395749864 1108 8 0.22981959790499928 1108 9 0.6158576562548816 1108 10-11 0.27438364820633154 1108 12-13 0.3873201704821554 1108 14-15 0.6060795792684672 1108 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-1.3466170519993135 2316 76-77 -1.392402495462111 2316 78-79 -1.4466161608708568 2316 80-81 -1.5809831237131284 2316 82-83 -1.3809183399387344 2316 84-85 -1.4380961972254127 2316 86-87 -1.8190874206902379 2316 88-89 -1.7377596758000102 2316 90-91 -2.017388268638939 2316 92-93 -1.5403563425963185 2316 94-95 -1.3405628916646428 2316 96-97 -1.2004298865372114 2316 98-99 -1.4393306992333699 2316 100-101 -1.8406344996954829 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2044.0 3 331.0 4 302.0 5 350.0 6 423.0 7 432.0 8 566.0 9 729.0 10 860.0 11 1261.0 12 2040.0 13 2753.0 14 3285.0 15 4581.0 16 6243.0 17 9044.0 18 7550.0 19 4167.0 20 3746.0 21 3895.0 22 4040.0 23 4387.0 24 4729.0 25 5279.0 26 6018.0 27 6862.0 28 7971.0 29 9719.0 30 11565.0 31 14565.0 32 18319.0 33 24356.0 34 33759.0 35 49163.0 36 69183.0 37 92258.0 38 65923.0 39 6198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.30171324482662 18.95687349229532 14.255091323826246 20.48632193905181 2 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26.261458724000764 33.51450055026468 20.295134512559766 46-47 20.314788113925463 26.630943267840436 32.62204213639452 20.43222648183958 48-49 20.299838797447403 26.78801095726441 32.0817675096409 20.830382735647284 50-51 20.519321084878737 27.297507508830755 31.639153928212398 20.544017478078107 52-53 20.62116613140242 27.34807479079605 31.34513579042509 20.68562328737644 54-55 20.21487107018459 27.452628040838622 31.388207710067523 20.94429317890927 56-57 20.451087608000073 27.731519475104015 31.150330775039166 20.667062141856746 58-59 20.51389217545229 27.127004078070303 31.37932200160418 20.979781744873215 60-61 20.333674507525814 27.18564076713991 31.88288196909761 20.597802756236675 62-63 20.411799116285405 27.178475310596074 31.96237707546406 20.447348497654456 64-65 20.24175313026043 27.16546606327424 31.883832272751956 20.70894853371337 66-67 20.22982452725222 27.26225834973449 31.558968863243546 20.948948259769747 68-69 20.321887268763266 27.139426426352586 31.70145975230929 20.837226552574855 70-71 20.219975000512285 27.419110264133934 31.391774758713957 20.969139976639823 72-73 20.271283833631163 27.46098168762398 31.0349279472761 21.23280653146876 74-75 20.101240399838098 27.85535329108152 30.93939409465158 21.104012214428806 76-77 20.246732166791684 27.563747853210856 30.954854920092306 21.234665059905154 78-79 20.398901560562745 27.508940190382507 30.827825766192245 21.2643324828625 80-81 20.284400689791557 27.568197863203636 30.68527286830125 21.462128578703556 82-83 20.35024593850564 27.466940967586932 30.786624141827783 21.39618895207964 84-85 20.23452899545531 27.233209436892725 30.76108203802132 21.771179529630647 86-87 20.332573448426015 27.29171896211675 30.65095668656028 21.724750902896954 88-89 20.244006401914834 27.096043067427082 30.704395066181128 21.955555464476955 90-91 20.016864978734347 27.054356256794403 31.045393022652966 21.88338574181828 92-93 20.013769955677954 27.029582864601583 31.101657927252834 21.85498925246763 94-95 19.910801282346828 27.09577540693201 30.887591788308267 22.105831522412895 96-97 19.97721176053495 27.09976617462923 30.7427080707665 22.18031399406932 98-99 19.914810947725645 27.481820221794834 30.298674005863024 22.3046948246165 100-101 20.258119505766096 26.794906862458856 30.864827375665506 22.082146256109542 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1013.0 1 689.5 2 439.0 3 568.5 4 479.5 5 378.0 6 758.5 7 1668.5 8 2675.0 9 3012.5 10 2404.0 11 1804.0 12 1744.5 13 1793.0 14 1811.5 15 1825.5 16 1859.5 17 1893.0 18 1900.5 19 1969.0 20 2141.5 21 2352.5 22 2637.5 23 3061.5 24 3520.5 25 4003.5 26 4689.5 27 5509.0 28 6038.0 29 6631.0 30 7454.0 31 8213.0 32 9185.5 33 9905.5 34 10418.5 35 10930.0 36 11506.0 37 12479.0 38 13289.5 39 13535.0 40 13597.0 41 14177.0 42 14836.5 43 15395.0 44 16240.5 45 16971.0 46 17050.0 47 17046.0 48 17495.5 49 17207.0 50 16061.0 51 15096.5 52 14281.0 53 13901.0 54 13557.5 55 12464.5 56 11178.5 57 9926.5 58 9004.5 59 8050.5 60 6664.5 61 5452.0 62 4417.0 63 3175.0 64 2199.5 65 1645.5 66 1179.5 67 851.0 68 603.0 69 404.0 70 276.5 71 201.0 72 147.0 73 116.5 74 95.5 75 75.0 76 53.0 77 36.0 78 25.5 79 17.0 80 9.0 81 5.0 82 6.0 83 4.0 84 3.5 85 2.0 86 0.5 87 2.0 88 2.5 89 1.5 90 0.5 91 0.5 92 1.0 93 1.0 94 1.0 95 1.5 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2863594711349653 2 0.24851911244927347 3 0.254655386830737 4 0.25056453724309463 5 0.25015545228433045 6 0.2045424793821181 7 0.1965653226862155 8 0.20065617227385782 9 0.20495156434088233 10-11 0.206178819217175 12-13 0.2082242440109962 14-15 0.2040311231836628 16-17 0.19697440764497973 18-19 0.19094040450320723 20-21 0.1917585744207357 22-23 0.19707667888467076 24-25 0.19605396648776016 26-27 0.20096298599293103 28-29 0.20505383558057336 30-31 0.20167888467076842 32-33 0.2014743421913863 34-35 0.20464475062180912 36-37 0.19728122136405288 38-39 0.18541775755989004 40-41 0.18337233276606885 42-43 0.19226993061919098 44-45 0.19411081293363006 46-47 0.2005539010341668 48-49 0.20464475062180912 50-51 0.19850847624034562 52-53 0.19942891739756513 54-55 0.19953118863725616 56-57 0.17907694069904437 58-59 0.16312262730723917 60-61 0.17907694069904437 62-63 0.17232703887943449 64-65 0.1794860256578086 66-67 0.17825877078151592 68-69 0.17754287210367847 70-71 0.18122463673255662 72-73 0.18817908103154862 74-75 0.194008541693939 76-77 0.19595169524806913 78-79 0.19042904830475194 80-81 0.18869043723000392 82-83 0.1774406008639874 84-85 0.17467927739232883 86-87 0.18071328053410132 88-89 0.18756545359340226 90-91 0.18459958764236156 92-93 0.17938375441811755 94-95 0.18091782301348344 96-97 0.18920179342845922 98-99 0.18869043723000392 100-101 0.16925890168870272 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 488896.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.70074480835625 #Duplication Level Percentage of deduplicated Percentage of total 1 81.92426239288037 51.367122699088085 2 10.557738323024576 13.23956112690734 3 3.179720414119061 5.981125147428002 4 1.4270053051979488 3.578971819255484 5 0.7365814118663174 2.309210156800436 6 0.4899931609126367 1.8433761684133843 7 0.3137618595539423 1.3771171600540932 8 0.2162503576192784 1.0847246790241722 9 0.18003847512121932 1.015969183583505 >10 0.9073794446876856 10.00246266595354 >50 0.04147157593234655 1.7849924390965155 >100 0.02253180071949658 2.2819156313706914 >500 0.0016327391825722147 0.648231932608309 >1k 0.0013061913460577718 2.149034227623756 >5k 3.2654783651444295E-4 1.3361849627927433 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6526 1.3348442204477027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4878 0.9977582144259719 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3348 0.6848082209713313 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1139 0.23297388401623248 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1131 0.23133754418117555 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 668 0.13663437622725488 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 647 0.1323389841602304 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 634 0.12967993192826288 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 620 0.1268163372169132 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 597 0.1221118601911245 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 499 0.10206669721167692 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.045424793821181E-4 0.0 0.0 0.0 0.0 3 2.045424793821181E-4 0.0 0.0 0.0 0.0 4 2.045424793821181E-4 0.0 0.0 0.0 0.0 5 2.045424793821181E-4 0.0 0.0 0.0 0.0 6 2.045424793821181E-4 0.0 0.0 0.0 0.0 7 2.045424793821181E-4 0.0 0.0 0.0 0.0 8 2.045424793821181E-4 0.0 0.0 0.0 0.0 9 2.045424793821181E-4 0.0 0.0 0.0 0.0 10-11 2.045424793821181E-4 0.0 0.0 0.0 0.0 12-13 2.045424793821181E-4 0.0 0.0 0.0 0.0 14-15 2.045424793821181E-4 0.0 0.0 0.0 0.0 16-17 2.045424793821181E-4 0.0 0.0 1.0227123969105904E-4 0.0 18-19 3.0681371907317716E-4 0.0 0.0 2.045424793821181E-4 0.0 20-21 4.090849587642362E-4 0.0 0.0 2.045424793821181E-4 0.0 22-23 4.090849587642362E-4 0.0 0.0 5.113561984552952E-4 0.0 24-25 6.136274381463542E-4 0.0 0.0 0.0010227123969105904 0.0 26-27 6.136274381463542E-4 0.0 0.0 0.002249967273203299 0.0 28-29 6.136274381463542E-4 0.0 0.0 0.0067499018196098964 0.0 30-31 6.136274381463542E-4 0.0 0.0 0.014727058515512502 0.0 32-33 6.136274381463542E-4 0.0 0.0 0.022806486451106167 0.0 34-35 6.136274381463542E-4 0.0 0.0 0.0317040843042283 0.0 36-37 6.136274381463542E-4 0.0 0.0 0.04203347951302526 0.0 38-39 0.0010227123969105904 0.0 0.0 0.05696508050791989 0.0 40-41 0.0010227123969105904 0.0 0.0 0.07557844613169262 0.0 42-43 0.0011249836366016494 0.0 0.0 0.09869174630187197 0.0 44-45 0.0012272548762927084 0.0 0.0 0.1176119256447179 0.0 46-47 0.0016363398350569447 0.0 0.0 0.15494092813195445 0.0 48-49 0.0016363398350569447 0.0 0.0 0.2062810904568661 0.0 50-51 0.0016363398350569447 0.0 0.0 0.22990574682550072 0.0 52-53 0.0016363398350569447 0.0 0.0 0.27705278832307895 0.0 54-55 0.0016363398350569447 0.0 0.0 0.3579493389187066 0.0 56-57 0.0016363398350569447 0.0 0.0 0.4737203822489855 0.0 58-59 0.0016363398350569447 0.0 0.0 0.6205818824453462 0.0 60-61 0.0016363398350569447 0.0 0.0 0.7848294933891871 0.0 62-63 0.0016363398350569447 0.0 0.0 0.9498952742505563 0.0 64-65 0.0016363398350569447 0.0 0.0 1.1263131627176333 0.0 66-67 0.0016363398350569447 0.0 0.0 1.3399577824322555 0.0 68-69 0.0016363398350569447 0.0 0.0 1.6051471069511716 0.0 70-71 0.0016363398350569447 0.0 0.0 1.7615198324388008 0.0 72-73 0.0017386110747480037 0.0 0.0 1.9881528995941877 0.0 74-75 0.0018408823144390627 0.0 0.0 2.2495581882445346 0.0 76-77 0.0018408823144390627 0.0 0.0 2.5287586726011257 0.0 78-79 0.0018408823144390627 0.0 0.0 2.8314815420866606 0.0 80-81 0.0020454247938211808 0.0 0.0 3.2660320395339704 0.0 82-83 0.0020454247938211808 0.0 0.0 3.7829108849325825 0.0 84-85 0.00214769603351224 0.0 0.0 4.423333387877994 0.0 86-87 0.002249967273203299 0.0 0.0 5.151811428197408 0.0 88-89 0.002249967273203299 0.0 0.0 6.035025854169394 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2095 0.0 52.175842 1 GTCGGGA 70 7.881681E-8 47.520508 2 GTATCAA 4045 0.0 44.52935 1 CGGGAGT 90 5.660677E-7 36.967983 4 ATCAACG 5070 0.0 34.96085 3 TATCAAC 5105 0.0 34.814243 2 TCAACGC 5185 0.0 34.10079 4 CAACGCA 5250 0.0 33.58805 5 GGTCGGG 100 1.2917553E-6 33.26777 1 CCATATA 100 1.2927903E-6 33.26436 2 AACGCAG 5380 0.0 32.85468 6 GTACATG 3120 0.0 29.094067 1 CGCAGAG 6080 0.0 29.07207 8 ACGCAGA 6095 0.0 29.000523 7 TACATGG 3105 0.0 28.313349 2 GCAGAGT 6265 0.0 27.53101 9 ACATGGG 3190 0.0 26.96305 3 TAGGCCA 150 1.052158E-6 25.349474 4 ATGGGAG 885 0.0 25.24206 5 CATGGGA 2305 0.0 24.744604 4 >>END_MODULE