FastQCFastQC Report
Thu 26 May 2016
SRR937768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937768_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488896
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52581.075484356591177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44140.9028505039926691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.6269226993061919No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10690.2186559104594842No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10390.21251963607802066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.20904241392852466No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.12599816729938473No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA4920.10063489985600209No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.047.634971
GTATCAA39850.036.5013961
TATCAAC51250.028.5500322
ATCAACG51750.028.0905863
TCAACGC52450.027.6251144
CAACGCA53100.027.2869555
TAGCGTA350.00839405327.1408164-65
AACGCAG54750.026.5459446
GTACATG32200.024.0629081
TACATGG32400.023.8996932
GCCGTAT801.858647E-623.75063992-93
ACGCAGA61500.023.7095957
CGCAGAG61400.023.5161448
GCACTGT3850.023.4397936
GTAAGGT2653.092282E-1123.30493
TGCACTG4150.022.8945245
CTATTAC1252.2420143E-422.816951
GAGTACT38000.022.74828512-13
ACATGGG32600.022.5872753
AATATGC2554.838512E-1022.3695581
Produced by FastQC (version 0.11.5)