##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937768_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 488896 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28748240934677 33.0 31.0 34.0 31.0 34.0 2 32.50080385194397 34.0 31.0 34.0 31.0 34.0 3 32.57432664615787 34.0 31.0 34.0 31.0 34.0 4 36.17781082275167 37.0 35.0 37.0 35.0 37.0 5 36.051254254483574 37.0 35.0 37.0 35.0 37.0 6 36.15940813588166 37.0 35.0 37.0 35.0 37.0 7 36.12697792577563 37.0 35.0 37.0 35.0 37.0 8 36.13286670375704 37.0 36.0 37.0 35.0 37.0 9 37.795950467993194 39.0 38.0 39.0 35.0 39.0 10-11 37.74965023236025 39.0 37.0 39.0 35.0 39.0 12-13 37.67106296635686 39.0 37.0 39.0 35.0 39.0 14-15 38.867521926953785 40.0 38.0 41.0 35.0 41.0 16-17 38.847845963149624 40.0 38.0 41.0 35.5 41.0 18-19 38.88732470709517 40.0 38.0 41.0 35.0 41.0 20-21 38.842799900183266 40.0 38.0 41.0 35.0 41.0 22-23 38.70011004385391 40.0 38.0 41.0 34.5 41.0 24-25 38.619268719727714 40.0 38.0 41.0 34.5 41.0 26-27 38.50545821606231 40.0 38.0 41.0 34.0 41.0 28-29 38.38467281384998 40.0 38.0 41.0 34.0 41.0 30-31 38.3612271321508 40.0 38.0 41.0 34.0 41.0 32-33 38.195439316337215 40.0 37.0 41.0 34.0 41.0 34-35 38.0355515283414 40.0 37.0 41.0 34.0 41.0 36-37 37.85465415957586 40.0 37.0 41.0 33.0 41.0 38-39 37.68525923713837 40.0 37.0 41.0 33.0 41.0 40-41 37.46277633688965 40.0 36.0 41.0 33.0 41.0 42-43 37.18294074813457 40.0 36.0 41.0 33.0 41.0 44-45 36.75681330998822 40.0 36.0 41.0 31.5 41.0 46-47 36.210711480560285 39.5 35.0 41.0 30.5 41.0 48-49 35.722084042413925 39.0 35.0 41.0 28.5 41.0 50-51 35.39905726371253 39.0 35.0 41.0 27.0 41.0 52-53 35.15459831947899 39.0 35.0 40.5 26.0 41.0 54-55 34.92124091831391 39.0 35.0 40.0 26.0 41.0 56-57 34.63827787504909 38.0 35.0 40.0 25.0 41.0 58-59 34.359853629401755 38.0 34.0 40.0 24.0 41.0 60-61 34.018671660230396 38.0 34.0 40.0 23.0 41.0 62-63 33.64582140168871 37.0 34.0 40.0 22.0 41.0 64-65 33.23420625245451 36.5 33.0 39.5 20.5 41.0 66-67 32.81091479578479 36.0 33.0 39.0 18.5 40.5 68-69 32.34445464884147 35.5 32.0 39.0 15.0 40.0 70-71 31.94459966945935 35.0 32.0 38.0 10.5 40.0 72-73 31.516309194593532 35.0 31.5 37.5 7.5 40.0 74-75 31.027563121809138 35.0 31.0 37.0 6.0 39.0 76-77 29.826826155255922 33.5 29.5 35.5 2.0 38.0 78-79 30.34191627667234 34.0 31.0 36.0 2.0 38.5 80-81 30.142469973164026 34.0 31.0 36.0 2.0 37.5 82-83 29.80904732294803 34.0 30.0 35.0 2.0 37.0 84-85 29.435767525199633 34.0 30.0 35.0 2.0 36.5 86-87 29.040543387550727 34.0 29.5 35.0 2.0 36.0 88-89 28.64436915008509 34.0 29.0 35.0 2.0 36.0 90-91 28.169922642034297 34.0 29.0 35.0 2.0 35.0 92-93 27.758000679081032 34.0 28.0 35.0 2.0 35.0 94-95 27.337159641314308 33.0 27.0 35.0 2.0 35.0 96-97 26.865626840882314 33.0 26.0 35.0 2.0 35.0 98-99 26.173479635750752 33.0 25.0 35.0 2.0 35.0 100-101 23.938732368438277 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10145769328986631 1101 2 0.15879357490226198 1101 3 0.12202390496077697 1101 4 -0.02985724841025217 1101 5 -0.0281798319153026 1101 6 0.014150179591823075 1101 7 -0.02464213713360408 1101 8 -0.10959360742079838 1101 9 0.0902649376164959 1101 10-11 0.10258252885008545 1101 12-13 0.15962979827483537 1101 14-15 0.25608939464240876 1101 16-17 -0.08729417227814196 1101 18-19 0.02426312128005037 1101 20-21 0.022056925739889266 1101 22-23 -0.024591892902158463 1101 24-25 -0.18445342227742145 1101 26-27 -0.06929965146096606 1101 28-29 -0.22385693026014764 1101 30-31 -0.029963574272258597 1101 32-33 0.07638053728410199 1101 34-35 -0.0031508200573071576 1101 36-37 0.3351978918545129 1101 38-39 0.43920020004374294 1101 40-41 0.32054377196287476 1101 42-43 0.3875631999102467 1101 44-45 0.5447994005197572 1101 46-47 0.5528534191219947 1101 48-49 0.7208138618940367 1101 50-51 0.765168631442215 1101 52-53 0.63128357482789 1101 54-55 0.5290020401652598 1101 56-57 0.6384025687757102 1101 58-59 0.4685931564510639 1101 60-61 0.6907545020304582 1101 62-63 0.7083742776719433 1101 64-65 0.5875975807350002 1101 66-67 0.7220152364130143 1101 68-69 0.5717750833954014 1101 70-71 0.40108638602821145 1101 72-73 0.516324051249935 1101 74-75 0.35198541476242795 1101 76-77 0.17043541696981634 1101 78-79 0.48610100134932566 1101 80-81 0.5772694296986352 1101 82-83 0.6293408579518776 1101 84-85 0.6092690743122589 1101 86-87 0.7921769324705892 1101 88-89 0.9202841935771637 1101 90-91 0.8150318638444389 1101 92-93 0.7573461421515546 1101 94-95 0.7745908564759745 1101 96-97 1.03690833444659 1101 98-99 1.4607823161187703 1101 100-101 2.0621322430069 1102 1 0.21497901076306647 1102 2 0.1854981432845193 1102 3 0.24145765093857108 1102 4 0.07588943481599841 1102 5 0.003352123744470248 1102 6 -0.11355140119069063 1102 7 -0.06204019033649644 1102 8 -0.054347672215392606 1102 9 0.16920392870749623 1102 10-11 0.021306066024699533 1102 12-13 0.15891166837178616 1102 14-15 0.03164727172786286 1102 16-17 -0.03324985297711436 1102 18-19 0.09846888156365452 1102 20-21 0.10257191679502142 1102 22-23 0.08756639757503848 1102 24-25 0.1692715122987778 1102 26-27 0.09130587926020439 1102 28-29 -0.07607311949372786 1102 30-31 -0.1726636829258439 1102 32-33 -0.09136593969463291 1102 34-35 -0.02390709080349751 1102 36-37 0.0870320748379001 1102 38-39 0.009550028693915635 1102 40-41 0.1828728144513576 1102 42-43 0.46678167131732096 1102 44-45 0.35852053894621605 1102 46-47 0.41351379972337554 1102 48-49 0.23406376960574704 1102 50-51 0.4119630519988178 1102 52-53 0.18438109851273055 1102 54-55 0.21322365759407091 1102 56-57 0.19489869266769944 1102 58-59 0.43918046864254023 1102 60-61 0.4093753638162312 1102 62-63 0.23757399251235256 1102 64-65 0.39838268129560817 1102 66-67 0.44624737545372994 1102 68-69 0.3824062456001016 1102 70-71 0.4176198138169269 1102 72-73 0.591199368654383 1102 74-75 0.4688993542541908 1102 76-77 0.3432558815049589 1102 78-79 0.5049449990238131 1102 80-81 0.7005792836963245 1102 82-83 0.7389076036800901 1102 84-85 0.6178432912816483 1102 86-87 0.6765961290319318 1102 88-89 0.6783842654648815 1102 90-91 0.7713327998793105 1102 92-93 1.1710054063262127 1102 94-95 1.000818215954915 1102 96-97 1.3312407127642985 1102 98-99 1.4736324324692625 1102 100-101 2.0177957546845455 1103 1 0.10908733439663365 1103 2 0.14744370904890047 1103 3 0.06105364555623538 1103 4 0.09172935160442819 1103 5 -0.10984863242416765 1103 6 0.06407393210962198 1103 7 -0.01035490838300035 1103 8 0.010321256353087449 1103 9 0.15306187466951116 1103 10-11 0.10431868809723 1103 12-13 0.21096677952692744 1103 14-15 0.21934137014535793 1103 16-17 0.09541273566220099 1103 18-19 0.11616702498805154 1103 20-21 0.12685929389697748 1103 22-23 0.10995922553016158 1103 24-25 0.15770939667762462 1103 26-27 0.2808532512439825 1103 28-29 0.16937294570517736 1103 30-31 0.1651794203374095 1103 32-33 0.2302764982062513 1103 34-35 0.24607895805028335 1103 36-37 0.15369626496973154 1103 38-39 0.23653765427486917 1103 40-41 0.2611791948305964 1103 42-43 0.48200080337440454 1103 44-45 0.48390171893250766 1103 46-47 0.6234463554247682 1103 48-49 0.5177574387572008 1103 50-51 0.6146748576403596 1103 52-53 0.5745220133446907 1103 54-55 0.6339993083036006 1103 56-57 0.875133468169814 1103 58-59 0.6846768900497011 1103 60-61 0.6081536066322215 1103 62-63 0.9341018906772476 1103 64-65 0.8811715897216033 1103 66-67 1.0593919968328294 1103 68-69 0.8745085760193803 1103 70-71 1.0740328007655933 1103 72-73 0.8880211489527206 1103 74-75 0.9697009260282989 1103 76-77 0.7147625227458683 1103 78-79 0.9571041246419121 1103 80-81 0.8874131990153415 1103 82-83 0.9423697993758715 1103 84-85 0.990708724633965 1103 86-87 0.9774868560600076 1103 88-89 1.0028557316560551 1103 90-91 1.0717926098465398 1103 92-93 1.281881521798777 1103 94-95 1.26653471875753 1103 96-97 1.48318933292164 1103 98-99 1.9194235615214943 1103 100-101 2.5361518308627566 1104 1 0.12755720269284154 1104 2 0.18433992158556833 1104 3 0.058104455538916966 1104 4 0.1245586763722244 1104 5 -0.09548917595257223 1104 6 0.027287400193436895 1104 7 0.15836174130928526 1104 8 0.0389896813916053 1104 9 0.22842638863637887 1104 10-11 0.15795947536035015 1104 12-13 0.020413240585952508 1104 14-15 0.11114579044845385 1104 16-17 0.10152264260776889 1104 18-19 0.11610783227223465 1104 20-21 0.21905456022067682 1104 22-23 0.2175097787209097 1104 24-25 0.254726257896813 1104 26-27 0.1512917125815676 1104 28-29 0.08349824275686046 1104 30-31 0.17356563539847514 1104 32-33 0.360342915915318 1104 34-35 0.3437373638511687 1104 36-37 0.17353017717300645 1104 38-39 0.01833241223759785 1104 40-41 0.3545026046792117 1104 42-43 0.4490469998071802 1104 44-45 0.5287001590303504 1104 46-47 0.5713071480848768 1104 48-49 0.5908726295231403 1104 50-51 0.5635967667795398 1104 52-53 0.5385145620263501 1104 54-55 0.6467910282872325 1104 56-57 0.7411820932448947 1104 58-59 0.6270301486957592 1104 60-61 0.5897707864475024 1104 62-63 0.6823622341690907 1104 64-65 0.4889919920579686 1104 66-67 0.46269460218280756 1104 68-69 0.5951529061406333 1104 70-71 0.629227396818834 1104 72-73 0.8606079576819106 1104 74-75 0.8681532106059855 1104 76-77 0.4973041535051905 1104 78-79 0.6505171940453707 1104 80-81 0.775330050659246 1104 82-83 0.6303841449046459 1104 84-85 0.5274900336634651 1104 86-87 0.5591945659112447 1104 88-89 0.7955680637620084 1104 90-91 0.7499966332102552 1104 92-93 0.8354082760358423 1104 94-95 0.8225789043789185 1104 96-97 1.0950400628200576 1104 98-99 0.8896087128546348 1104 100-101 1.3873761625508791 1105 1 0.07589025315497366 1105 2 0.08851991986738739 1105 3 0.046793797999562514 1105 4 0.1572669691082993 1105 5 0.04058235065094351 1105 6 0.11276688784034405 1105 7 0.06929293236468226 1105 8 0.06692478445101813 1105 9 0.02050557581120671 1105 10-11 0.08014894039852294 1105 12-13 -0.02293939084962915 1105 14-15 0.24200620084219082 1105 16-17 0.07269911442855204 1105 18-19 0.14448505254927824 1105 20-21 0.07917684439518524 1105 22-23 0.12026703960569307 1105 24-25 0.07725705418973661 1105 26-27 0.08962578976139923 1105 28-29 0.018346718081062363 1105 30-31 0.12947125280155092 1105 32-33 0.17022727397579018 1105 34-35 0.04879279613075482 1105 36-37 -0.07575478727951435 1105 38-39 -0.09720371251069793 1105 40-41 0.11472735109042276 1105 42-43 0.154694366276253 1105 44-45 0.027396856142338777 1105 46-47 0.179872069121906 1105 48-49 -0.007211389510949573 1105 50-51 0.16761584277551123 1105 52-53 0.27608656322488656 1105 54-55 0.12246305693475534 1105 56-57 0.39171357681006924 1105 58-59 0.2709916865931632 1105 60-61 0.09652302689222125 1105 62-63 0.16359739652668992 1105 64-65 0.17666551258918872 1105 66-67 0.01544798668439995 1105 68-69 -0.06638331499340211 1105 70-71 0.2527991591483456 1105 72-73 0.3485084037802757 1105 74-75 0.4124717948251231 1105 76-77 0.16003471972953065 1105 78-79 0.47193536359627686 1105 80-81 0.6498358118027987 1105 82-83 0.7955547371950438 1105 84-85 0.8234937968981733 1105 86-87 0.7793647496099005 1105 88-89 0.6747935833545071 1105 90-91 0.675197105081331 1105 92-93 0.7992227628131836 1105 94-95 1.100481005797679 1105 96-97 1.2777182873946487 1105 98-99 1.182465272009292 1105 100-101 1.629289712919764 1106 1 -0.01079245024825326 1106 2 0.04925062696320026 1106 3 -0.0346429397376653 1106 4 -0.1105790782207805 1106 5 -0.21425529958251133 1106 6 -0.15865635773476328 1106 7 -0.15011447767277275 1106 8 -0.21922000775193595 1106 9 -0.10659591720304462 1106 10-11 0.0617325829272346 1106 12-13 -0.16632689994796124 1106 14-15 -0.18412864976761512 1106 16-17 -0.07780062815519528 1106 18-19 -0.07240145351331506 1106 20-21 0.040748592517822146 1106 22-23 0.009411618774500141 1106 24-25 0.07721489118311808 1106 26-27 0.08445971189742352 1106 28-29 0.05005551816627474 1106 30-31 -0.13421397150564474 1106 32-33 -0.09132538983859462 1106 34-35 -0.5351715028981658 1106 36-37 -0.26914096491447737 1106 38-39 -0.15304306701725778 1106 40-41 -0.3271919977352269 1106 42-43 -0.34960182218794955 1106 44-45 -0.45688299970456114 1106 46-47 -0.43504431497864715 1106 48-49 -0.7618981405628062 1106 50-51 -0.8216902872818324 1106 52-53 -0.7322483974525653 1106 54-55 -0.7394242480058608 1106 56-57 -0.812699336609306 1106 58-59 -0.6813641159241826 1106 60-61 -0.6732314512629074 1106 62-63 -0.3743537476441716 1106 64-65 -0.6893959300069881 1106 66-67 -0.6830576675966995 1106 68-69 -1.0358351071430931 1106 70-71 -0.8702790953919433 1106 72-73 -0.6007095834021676 1106 74-75 -0.3094365472366931 1106 76-77 -0.5098134046824079 1106 78-79 -0.08904787771814426 1106 80-81 -0.16430521679703602 1106 82-83 -0.2611278774439505 1106 84-85 -0.21940817189232575 1106 86-87 -0.35643341829650765 1106 88-89 -0.24704285968548945 1106 90-91 -0.07234412590093342 1106 92-93 -0.2778223649185314 1106 94-95 -0.43829786476633004 1106 96-97 -0.14231402278305794 1106 98-99 0.12458434033998955 1106 100-101 0.6120184139441065 1107 1 0.14857907710080553 1107 2 0.08771216542474036 1107 3 -0.010411553146724373 1107 4 0.06394864125076083 1107 5 -0.27543569222357434 1107 6 -0.07000343762116756 1107 7 0.12649374556466597 1107 8 0.028125343525999824 1107 9 0.2331991192212044 1107 10-11 0.04631401273338298 1107 12-13 0.03233960208932274 1107 14-15 -0.001822192180263471 1107 16-17 -0.10413103931706047 1107 18-19 -0.006698897642223756 1107 20-21 -0.019598363896548676 1107 22-23 0.10693909130197454 1107 24-25 -0.04229060332237822 1107 26-27 -0.1905742429118078 1107 28-29 -0.1715459141093163 1107 30-31 -0.23490541489372418 1107 32-33 -0.0886398731081286 1107 34-35 0.08292949722530807 1107 36-37 -0.03526949930144241 1107 38-39 0.06301755775123041 1107 40-41 0.11965329180668505 1107 42-43 -0.1498086378442025 1107 44-45 -0.30314488388778926 1107 46-47 -0.20984675972491829 1107 48-49 -0.3055609384390934 1107 50-51 -0.20152113059582888 1107 52-53 -0.21223049400769867 1107 54-55 -0.21226040061729634 1107 56-57 -0.2853502811703592 1107 58-59 -0.5418407179738196 1107 60-61 -0.46711032519795737 1107 62-63 -0.6964614152574526 1107 64-65 -0.5059164881005813 1107 66-67 -0.4667655327652369 1107 68-69 -0.4800103139587506 1107 70-71 -0.1397105066772859 1107 72-73 -0.3117695908104885 1107 74-75 -0.024185281648346546 1107 76-77 -0.08689082164746509 1107 78-79 0.05998286681821696 1107 80-81 0.06425695137187404 1107 82-83 0.03532847519217697 1107 84-85 -0.19202824844498778 1107 86-87 0.29282883630282797 1107 88-89 0.4771977873079649 1107 90-91 0.5214174496022146 1107 92-93 0.7133648186843793 1107 94-95 0.29298068814143363 1107 96-97 0.8829668760933451 1107 98-99 1.1761802163740676 1107 100-101 1.65938537282792 1108 1 0.00997946076756051 1108 2 0.1456389946930301 1108 3 0.16145060086807206 1108 4 0.07110813700301577 1108 5 -0.13197172949406166 1108 6 0.04263016062493108 1108 7 -0.02930236295821942 1108 8 -0.05535643230299314 1108 9 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0.24520255833947147 2316 88-89 0.039451051808420345 2316 90-91 0.2862342326774261 2316 92-93 0.2196407575445889 2316 94-95 0.31654076507230045 2316 96-97 0.058136427667392354 2316 98-99 -0.2429411521855016 2316 100-101 -0.7396175626918726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 3.0 5 12.0 6 33.0 7 62.0 8 143.0 9 272.0 10 468.0 11 808.0 12 1274.0 13 1863.0 14 2433.0 15 3567.0 16 5340.0 17 7885.0 18 7190.0 19 3891.0 20 3018.0 21 2955.0 22 3098.0 23 2943.0 24 3331.0 25 3893.0 26 4362.0 27 5276.0 28 6299.0 29 7909.0 30 9652.0 31 12258.0 32 15951.0 33 21530.0 34 30537.0 35 46013.0 36 70299.0 37 108247.0 38 87897.0 39 8181.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.43434310363173 20.25467983924435 14.167205520041254 20.14377153708266 2 16.305308286424925 22.521354234847493 39.31388270716062 21.85945477156696 3 20.341954117031026 27.23217207749705 29.89511061657285 22.53076318889907 4 12.702497054588296 18.09321409870402 36.53844580442466 32.66584304228302 5 12.6525076304958 39.83548405574149 32.55950968438797 14.95249862937475 6 29.94235992931012 36.74012468909543 17.10036490378322 16.21715047781123 7 26.98631201727975 35.210965113234714 20.045367521926956 17.757355347558583 8 27.79077758868962 33.36374198193481 19.32169622987302 19.523784199502554 9 28.13972705851551 16.118970087707815 20.236001112711087 35.50530174106559 10-11 25.531196818955358 27.513417986647465 25.66629712658725 21.28908806780992 12-13 26.130241082642296 25.767676189151484 27.48027115149383 20.62181157671239 14-15 22.915712135096218 26.253845398612384 27.36215882314439 23.468283643147007 16-17 20.6167569380809 29.944814439062707 27.781982262076188 21.65644636078021 18-19 20.549973818562638 26.93507412619453 32.332029715931405 20.18292233931143 20-21 21.430631463542348 25.86521468778636 32.94166857572981 19.762485272941486 22-23 21.11209973511695 26.389511040202905 32.68534143323208 19.81304779144806 24-25 20.731487807172925 26.397542207513286 32.973098463061525 19.897871522252267 26-27 20.336395034051662 26.698773444715012 32.78890219569044 20.17592932554289 28-29 20.215489248549 26.992713047480247 32.93047991613612 19.861317787834626 30-31 20.278649644554488 26.68628239074563 32.86247413802801 20.17259382667186 32-33 20.152445509883492 26.818075827987958 32.691410852205784 20.338067809922762 34-35 20.566337216913208 26.846302690142686 32.80585236942008 19.781507723524022 36-37 20.864786033007054 26.562015809104402 32.565752804024584 20.007445353863964 38-39 20.53064455426103 26.98252798141118 32.46794819348082 20.01887927084697 40-41 20.565007690797223 27.06434497316403 32.41824437099097 19.95240296504778 42-43 20.46238174775514 27.288051166182697 31.93774631089818 20.311820775163987 44-45 20.542610289304882 27.57846249509098 31.672482491163766 20.206444724440374 46-47 20.98350160361304 27.92955966095039 30.779756839900514 20.307181895536065 48-49 20.951695248069118 28.166113038355807 30.292638925251996 20.58955278832308 50-51 21.08873870925514 28.717252749050925 29.896746956407906 20.297261585286034 52-53 21.29606429185787 28.82579201485798 29.48360130130058 20.394542391983563 54-55 20.76443660819479 29.053725127634504 29.568865361958373 20.61297290221233 56-57 20.975524446917134 29.269721985862024 29.28127863594711 20.473474931273728 58-59 20.840628681764628 28.52007379892656 29.594433171881136 21.044864347427676 60-61 20.653165499410918 28.84918264825239 30.017324748003666 20.480327104333025 62-63 20.784277228694855 28.657833158790417 30.238128354496663 20.319761258018065 64-65 20.468565911768554 28.858284788584893 30.044426626521798 20.628722673124756 66-67 20.51162210367849 28.9119771894227 29.846327235240217 20.730073471658596 68-69 20.726187164550332 28.774422372038227 29.723704018850633 20.775686444560808 70-71 20.594666350307634 29.195063980887554 29.394901983243884 20.815367685560936 72-73 20.67668788453986 29.07315666317581 29.09749721822228 21.15265823406205 74-75 20.509372136405286 29.535320395339703 28.90553410132216 21.049773366932843 76-77 20.563371350962168 29.210097853122136 29.12347411310381 21.103056682811886 78-79 20.785095398612384 29.259290319413534 28.759695313522716 21.19591896845137 80-81 20.701232982065715 29.543093009556227 28.643412913993977 21.11226109438408 82-83 20.624959730658187 29.51960081448898 28.589852023591956 21.265587431260872 84-85 20.407121767330647 29.337966907038414 28.761872424679712 21.493038900951227 86-87 20.40638499803639 29.220120434611864 28.708457422437494 21.66503714491426 88-89 20.205728825762534 29.212961447833486 28.61160655845006 21.96970316795392 90-91 20.156470704956885 29.324602558477064 28.73198178156493 21.78694495500112 92-93 20.085807939535574 29.03887948535691 28.91993474914729 21.955377825960227 94-95 19.713088264170704 29.3889702513418 28.82586480560283 22.07207667888467 96-97 19.880404012305274 29.434890037963086 28.53244861892918 22.15225733080246 98-99 19.63458411315313 29.646856687018687 28.128125671156383 22.5904335286718 100-101 19.3873000286404 29.66991939773332 28.41270815433084 22.530072419295447 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 855.0 1 598.5 2 374.0 3 473.0 4 430.0 5 380.5 6 704.5 7 1340.0 8 1963.5 9 2270.0 10 2037.5 11 1764.5 12 1757.5 13 1746.0 14 1875.0 15 1930.5 16 1920.5 17 1916.0 18 1887.5 19 1950.0 20 2039.0 21 2239.0 22 2555.5 23 2933.5 24 3342.5 25 3917.0 26 4537.5 27 5228.0 28 5934.5 29 6625.5 30 7456.0 31 8053.5 32 8865.5 33 9532.5 34 10234.5 35 10896.5 36 11649.0 37 12821.5 38 13475.5 39 13557.0 40 13473.5 41 14130.5 42 14741.5 43 15429.0 44 16391.0 45 16996.0 46 16890.0 47 16749.0 48 17603.5 49 17203.0 50 16293.0 51 15571.0 52 14378.0 53 14046.0 54 14002.0 55 13121.0 56 11527.5 57 10265.5 58 9586.5 59 8618.0 60 6985.5 61 5626.0 62 4601.0 63 3283.0 64 2177.5 65 1558.5 66 1125.5 67 848.5 68 607.0 69 393.0 70 282.5 71 194.5 72 144.0 73 120.0 74 82.0 75 67.0 76 51.0 77 30.0 78 23.0 79 14.0 80 8.5 81 7.5 82 3.5 83 2.0 84 3.0 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04172666579395209 2 0.0 3 0.0 4 0.0 5 0.01390888859798403 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003681764628878126 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.0454247938211806E-4 24-25 4.090849587642361E-4 26-27 0.0013295261159837674 28-29 0.0017386110747480037 30-31 5.113561984552952E-4 32-33 0.0 34-35 0.0 36-37 1.0227123969105903E-4 38-39 0.0 40-41 0.0 42-43 0.011761192564471789 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.0227123969105903E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.0227123969105903E-4 84-85 1.0227123969105903E-4 86-87 0.0 88-89 0.0 90-91 0.010329395208796964 92-93 0.002761323471658594 94-95 0.0 96-97 0.0 98-99 2.0454247938211806E-4 100-101 0.015545228433040974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 488896.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.64177075084491 #Duplication Level Percentage of deduplicated Percentage of total 1 80.54709729895058 48.8451860904896 2 11.216060865265359 13.603235834378902 3 3.4088044519496346 6.201478143287678 4 1.5476870657433313 3.754179369394197 5 0.8777683082082094 2.6614712259359607 6 0.5220062862843963 1.8993231326014968 7 0.3545596116803493 1.5050785882319835 8 0.24941193537672923 1.2099825126112127 9 0.18515572566218327 1.0105353961931205 >10 1.0092413848327135 10.839046236630976 >50 0.057409066937915275 2.356013520219387 >100 0.022420108783106678 2.718984194811193 >500 3.3969861792585874E-4 0.1268955423898549 >1k 0.0016984930896292937 2.1854461188538483 >5k 3.3969861792585874E-4 1.083144093970547 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5258 1.075484356591177 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4414 0.9028505039926691 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3065 0.6269226993061919 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1069 0.2186559104594842 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1039 0.21251963607802066 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1022 0.20904241392852466 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 616 0.12599816729938473 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 492 0.10063489985600209 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.0227123969105904E-4 0.0 18-19 0.0 0.0 0.0 2.045424793821181E-4 0.0 20-21 0.0 0.0 0.0 2.045424793821181E-4 0.0 22-23 0.0 0.0 0.0 5.113561984552952E-4 0.0 24-25 0.0 0.0 0.0 0.0010227123969105904 0.0 26-27 0.0 0.0 0.0 0.002249967273203299 0.0 28-29 0.0 0.0 0.0 0.006545359340227779 0.0 30-31 2.045424793821181E-4 0.0 0.0 0.014113431077366147 0.0 32-33 2.045424793821181E-4 0.0 0.0 0.021988316533577693 0.0 34-35 2.045424793821181E-4 0.0 0.0 0.031090456866081948 0.0 36-37 2.045424793821181E-4 0.0 0.0 0.041419852074878916 0.0 38-39 2.045424793821181E-4 0.0 0.0 0.05655599554915565 0.0 40-41 2.045424793821181E-4 0.0 0.0 0.07496481869354628 0.0 42-43 3.0681371907317716E-4 0.0 0.0 0.09858947506218091 0.0 44-45 4.090849587642362E-4 0.0 0.0 0.11802101060348213 0.0 46-47 6.136274381463542E-4 0.0 0.0 0.15616818300824714 0.0 48-49 6.136274381463542E-4 0.0 0.0 0.2072015316140856 0.0 50-51 6.136274381463542E-4 0.0 0.0 0.2314398154208666 0.0 52-53 6.136274381463542E-4 0.0 0.0 0.27991638303442856 0.0 54-55 6.136274381463542E-4 0.0 0.0 0.3609152048697474 0.0 56-57 6.136274381463542E-4 0.0 0.0 0.4777089605969368 0.0 58-59 6.136274381463542E-4 0.0 0.0 0.6251840882314439 0.0 60-61 6.136274381463542E-4 0.0 0.0 0.7905566828118864 0.0 62-63 6.136274381463542E-4 0.0 0.0 0.9560315486320199 0.0 64-65 6.136274381463542E-4 0.0 0.0 1.1350062180913731 0.0 66-67 6.136274381463542E-4 0.0 0.0 1.3524348736745648 0.0 68-69 6.136274381463542E-4 0.0 0.0 1.6209991491032858 0.0 70-71 6.136274381463542E-4 0.0 0.0 1.7796218418641183 0.0 72-73 6.136274381463542E-4 0.0 0.0 2.009425317449928 0.0 74-75 6.136274381463542E-4 0.0 0.0 2.275125998167299 0.0 76-77 6.136274381463542E-4 0.0 0.0 2.558212789632151 0.0 78-79 6.136274381463542E-4 0.0 0.0 2.865537864903783 0.0 80-81 8.181699175284724E-4 0.0 0.0 3.306735992931012 0.0 82-83 8.181699175284724E-4 0.0 0.0 3.82995565519047 0.0 84-85 8.181699175284724E-4 0.0 0.0 4.479480298468387 0.0 86-87 8.181699175284724E-4 0.0 0.0 5.215935495483702 0.0 88-89 8.181699175284724E-4 0.0 0.0 6.113365623772745 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2385 0.0 47.63497 1 GTATCAA 3985 0.0 36.501396 1 TATCAAC 5125 0.0 28.550032 2 ATCAACG 5175 0.0 28.090586 3 TCAACGC 5245 0.0 27.625114 4 CAACGCA 5310 0.0 27.286955 5 TAGCGTA 35 0.008394053 27.14081 64-65 AACGCAG 5475 0.0 26.545944 6 GTACATG 3220 0.0 24.062908 1 TACATGG 3240 0.0 23.899693 2 GCCGTAT 80 1.858647E-6 23.750639 92-93 ACGCAGA 6150 0.0 23.709595 7 CGCAGAG 6140 0.0 23.516144 8 GCACTGT 385 0.0 23.439793 6 GTAAGGT 265 3.092282E-11 23.3049 3 TGCACTG 415 0.0 22.894524 5 CTATTAC 125 2.2420143E-4 22.81695 1 GAGTACT 3800 0.0 22.748285 12-13 ACATGGG 3260 0.0 22.587275 3 AATATGC 255 4.838512E-10 22.369558 1 >>END_MODULE