FastQCFastQC Report
Thu 26 May 2016
SRR937767_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937767_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220935
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75620.34048722722637087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61580.2772706090002634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.16141850166709065No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27540.12400182805890313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58200.039.3024861
GTATCAA88400.033.5683671
ATCAACG110550.026.7492683
TCAACGC111200.026.5507554
CAACGCA112900.026.1521475
TATCAAC114950.025.766152
AACGCAG115500.025.5403736
GCGTACA1452.3199958E-522.9509412
GAGTACT69700.022.5245612-13
GTACATG68500.022.354411
CGCAGAG129900.022.160088
ACGCAGA130700.022.061787
ACCGTAT1100.002877653821.5916168
TACATGG70000.021.4614722
AGAGTAC107950.021.05581310-11
GTACTTT74950.020.85054414-15
GCAGAGT135750.020.6452459
CCCTATA2551.1814336E-820.5079842
ACATGGG70800.020.4136473
AGTACTT74250.020.40853712-13
Produced by FastQC (version 0.11.5)