##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937767_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2220935 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.749268663873547 33.0 31.0 34.0 30.0 34.0 2 31.898799829801412 33.0 31.0 34.0 30.0 34.0 3 31.922546585109426 33.0 31.0 34.0 30.0 34.0 4 35.52790468879098 37.0 35.0 37.0 33.0 37.0 5 35.5012073743716 37.0 35.0 37.0 33.0 37.0 6 35.56632454349182 37.0 35.0 37.0 33.0 37.0 7 35.503945860639774 37.0 35.0 37.0 33.0 37.0 8 35.490460549273166 37.0 35.0 37.0 33.0 37.0 9 37.137225537892824 39.0 37.0 39.0 34.0 39.0 10-11 37.00817921280902 39.0 37.0 39.0 33.0 39.0 12-13 36.89116385666397 39.0 37.0 39.0 33.0 39.0 14-15 38.06408967394363 40.0 38.0 41.0 33.0 41.0 16-17 37.97034244586176 40.0 38.0 41.0 33.0 41.0 18-19 37.889361687757635 40.0 38.0 41.0 33.0 41.0 20-21 37.781253165896345 40.0 37.5 41.0 32.5 41.0 22-23 37.69395975118587 40.0 37.0 41.0 32.0 41.0 24-25 37.72220618793436 40.0 37.0 41.0 32.0 41.0 26-27 37.64488042198444 40.0 37.0 41.0 32.0 41.0 28-29 37.52427175941664 40.0 37.0 41.0 32.0 41.0 30-31 37.30206332017821 39.5 37.0 41.0 31.5 41.0 32-33 37.18187249964542 39.0 36.5 41.0 31.0 41.0 34-35 37.20405775045195 40.0 37.0 41.0 31.0 41.0 36-37 37.08147627012947 40.0 36.0 41.0 31.0 41.0 38-39 36.94905411459588 39.5 36.0 41.0 31.0 41.0 40-41 36.737419600303475 39.0 36.0 41.0 30.5 41.0 42-43 36.48214873465455 39.0 36.0 41.0 30.0 41.0 44-45 36.26216480896559 39.0 35.0 41.0 30.0 41.0 46-47 35.93379995362314 39.0 35.0 40.5 28.5 41.0 48-49 35.634581156134686 39.0 35.0 40.0 27.5 41.0 50-51 34.72606199641142 38.0 34.0 39.5 26.0 40.5 52-53 34.81647842012485 38.0 34.0 39.5 26.5 40.5 54-55 35.10864050501253 38.0 35.0 40.0 26.5 41.0 56-57 34.863815239977754 38.0 34.0 40.0 26.0 41.0 58-59 34.59881198684337 38.0 34.0 40.0 26.0 41.0 60-61 34.25690058466367 38.0 34.0 40.0 25.0 41.0 62-63 33.86813751865768 37.0 33.0 40.0 23.5 41.0 64-65 33.45130721970702 36.5 33.0 39.0 22.5 41.0 66-67 32.993067559383775 36.0 32.0 39.0 22.0 40.5 68-69 32.46767780236702 35.5 32.0 39.0 20.0 40.0 70-71 31.99907966689705 35.0 31.0 38.0 18.5 40.0 72-73 31.52713046532204 35.0 31.0 37.0 17.5 39.0 74-75 30.985131712544494 35.0 30.5 36.5 14.0 39.0 76-77 30.50577999806388 34.0 30.0 36.0 9.5 39.0 78-79 29.95511147332092 34.0 29.5 35.5 7.0 37.5 80-81 29.41274035485055 34.0 29.0 35.0 3.5 37.0 82-83 28.93579303311443 34.0 29.0 35.0 2.0 36.5 84-85 28.456218664661506 34.0 27.5 35.0 2.0 36.0 86-87 27.95219648481383 33.0 26.5 35.0 2.0 35.5 88-89 27.49646027461407 33.0 26.0 35.0 2.0 35.0 90-91 26.965108163903942 33.0 25.5 35.0 2.0 35.0 92-93 26.344628050798427 32.0 24.5 34.5 2.0 35.0 94-95 25.610711704754976 32.0 22.0 34.0 2.0 35.0 96-97 24.78450449923118 31.5 19.0 34.0 2.0 35.0 98-99 23.734266423826 31.0 2.0 34.0 2.0 35.0 100-101 20.863634685391514 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08835566568435027 1101 2 0.22669559549574103 1101 3 0.21247815134331915 1101 4 0.10876385982430037 1101 5 0.06111095474314254 1101 6 0.14841960474588234 1101 7 0.12654625811774878 1101 8 0.1900201190094748 1101 9 0.21815057736724697 1101 10-11 0.18668550718086152 1101 12-13 0.18456233792787202 1101 14-15 0.340292634886012 1101 16-17 0.3631303806894337 1101 18-19 0.40422072325381464 1101 20-21 0.42151395035629236 1101 22-23 0.2662296845363983 1101 24-25 0.2976746236261931 1101 26-27 0.31753271938101335 1101 28-29 0.2854826761351674 1101 30-31 0.41206335299205676 1101 32-33 0.35472893128913796 1101 34-35 0.45630691807720325 1101 36-37 0.38347925663844507 1101 38-39 0.3337712305062581 1101 40-41 0.43881510190266226 1101 42-43 0.4509293827203109 1101 44-45 0.6812461832437009 1101 46-47 0.8016069412186013 1101 48-49 0.7261075779113071 1101 50-51 0.616824135900977 1101 52-53 0.594824082841896 1101 54-55 0.6849379664637709 1101 56-57 0.6048833141257504 1101 58-59 0.4983809189717263 1101 60-61 0.6313503239018061 1101 62-63 0.6473467870090488 1101 64-65 0.7559188383308992 1101 66-67 0.8428525949627286 1101 68-69 0.6669037840081558 1101 70-71 0.8286296533803821 1101 72-73 0.6013989860212945 1101 74-75 0.6184062710453766 1101 76-77 0.7364744274760433 1101 78-79 0.7730567577649659 1101 80-81 0.8509154063176112 1101 82-83 0.8993146177960867 1101 84-85 0.9698640913990069 1101 86-87 1.0987223014733587 1101 88-89 1.0361404592949306 1101 90-91 1.2082048816398832 1101 92-93 0.9917808948609057 1101 94-95 1.0650556085119511 1101 96-97 1.2909806025181219 1101 98-99 1.4956003457485672 1101 100-101 1.5099581046876445 1102 1 0.11070026700839009 1102 2 0.2798383298875251 1102 3 0.3077815278348126 1102 4 0.12793389517102582 1102 5 0.14249357821258712 1102 6 0.19531475579944413 1102 7 0.12999969221855423 1102 8 0.18139083004507484 1102 9 0.3134133344121821 1102 10-11 0.202107617439232 1102 12-13 0.33872680762928553 1102 14-15 0.2862359629466198 1102 16-17 0.5061889469010836 1102 18-19 0.38515340823828126 1102 20-21 0.5030133547345983 1102 22-23 0.3811795937285041 1102 24-25 0.32933162364572155 1102 26-27 0.30363891579946767 1102 28-29 0.3488958813610239 1102 30-31 0.42185107732662175 1102 32-33 0.4721480341461728 1102 34-35 0.41957404952407273 1102 36-37 0.5834210614698137 1102 38-39 0.5638452438247583 1102 40-41 0.4411253329257647 1102 42-43 0.49305799794870353 1102 44-45 0.4481956237498821 1102 46-47 0.46601805688638365 1102 48-49 0.5900656305213019 1102 50-51 0.48868345201010044 1102 52-53 0.5881132815842562 1102 54-55 0.5858081599919984 1102 56-57 0.5470552828019066 1102 58-59 0.5298020137439252 1102 60-61 0.5457854597230707 1102 62-63 0.5508189685467215 1102 64-65 0.6434678062454395 1102 66-67 0.6350306174987139 1102 68-69 0.55334001342991 1102 70-71 0.5065971187659741 1102 72-73 0.5540988122169281 1102 74-75 0.5957214818563976 1102 76-77 0.6846572930998853 1102 78-79 0.8031163199342011 1102 80-81 0.8211124496905882 1102 82-83 0.8435738011108711 1102 84-85 0.8816741954863367 1102 86-87 0.9561496063340229 1102 88-89 1.0357948034275708 1102 90-91 1.065151002287454 1102 92-93 0.9854278571902739 1102 94-95 1.199641114399821 1102 96-97 1.3049318201250912 1102 98-99 1.5330819400618445 1102 100-101 1.7352472253156854 1103 1 0.24641487210877244 1103 2 0.33683384357748025 1103 3 0.3460234706107599 1103 4 0.2085021611103457 1103 5 0.21788644085598463 1103 6 0.17395584602743952 1103 7 0.2610178312196396 1103 8 0.21198319189107195 1103 9 0.25600843872013 1103 10-11 0.28481346355332704 1103 12-13 0.32894138540441276 1103 14-15 0.43524703712724033 1103 16-17 0.4063106684915141 1103 18-19 0.43929616530682836 1103 20-21 0.49840557150833575 1103 22-23 0.4540165513842922 1103 24-25 0.45198525368164866 1103 26-27 0.4596432679673583 1103 28-29 0.4193783956819388 1103 30-31 0.4201616313337482 1103 32-33 0.5778182729688979 1103 34-35 0.510099886794734 1103 36-37 0.4785504589727836 1103 38-39 0.494351406911818 1103 40-41 0.5538457359484354 1103 42-43 0.6346475328728616 1103 44-45 0.49474295357564557 1103 46-47 0.6150892466279814 1103 48-49 0.52213554342827 1103 50-51 0.4582237418565285 1103 52-53 0.4383882730140627 1103 54-55 0.4903347126875133 1103 56-57 0.4091213701731675 1103 58-59 0.49276362424476616 1103 60-61 0.6214297147124199 1103 62-63 0.5777966959797922 1103 64-65 0.643201012392133 1103 66-67 0.8412155647609652 1103 68-69 0.7203435259672375 1103 70-71 0.7673115996606512 1103 72-73 0.6448939274549872 1103 74-75 0.5572748480794019 1103 76-77 0.6108224668139819 1103 78-79 0.7297636601932425 1103 80-81 0.6579985629959992 1103 82-83 0.6617967652551684 1103 84-85 0.6793845875292241 1103 86-87 0.854612933357707 1103 88-89 1.0161489387322966 1103 90-91 0.9747769443877203 1103 92-93 0.816256458286265 1103 94-95 1.169215188197878 1103 96-97 1.4973455841044405 1103 98-99 1.5864372329637355 1103 100-101 1.2864092938871714 1104 1 0.2549472376712316 1104 2 0.2517091169641219 1104 3 0.1773825273419405 1104 4 0.1804822592011135 1104 5 0.3051956332154546 1104 6 0.22579055328463227 1104 7 0.34866144952065525 1104 8 0.2807698412771842 1104 9 0.3572013747134051 1104 10-11 0.3408484818153923 1104 12-13 0.46281910862058595 1104 14-15 0.5511417653958404 1104 16-17 0.4714809385883214 1104 18-19 0.4136827414270172 1104 20-21 0.5355915029483924 1104 22-23 0.46673796781890786 1104 24-25 0.4012444683174152 1104 26-27 0.31371457538512004 1104 28-29 0.30449417048979655 1104 30-31 0.346358311321147 1104 32-33 0.4286521045630991 1104 34-35 0.3656020755768594 1104 36-37 0.44778355385404467 1104 38-39 0.47885680745913106 1104 40-41 0.4841144552622296 1104 42-43 0.3347353994417901 1104 44-45 0.5099700310419806 1104 46-47 0.335300847041907 1104 48-49 0.3159379288498414 1104 50-51 0.35468513255047895 1104 52-53 0.3800463350626515 1104 54-55 0.43682393467614844 1104 56-57 0.4957232385294503 1104 58-59 0.5484071652262159 1104 60-61 0.47333494793416264 1104 62-63 0.6326856204006575 1104 64-65 0.7257109485209057 1104 66-67 0.7033206015309332 1104 68-69 0.5436868695585488 1104 70-71 0.44175047909728704 1104 72-73 0.5115425216086464 1104 74-75 0.5654206157520854 1104 76-77 0.4398116409470525 1104 78-79 0.5263922450910776 1104 80-81 0.6412391691364512 1104 82-83 0.7728620226036256 1104 84-85 0.8634572611764213 1104 86-87 0.8176080085509945 1104 88-89 0.7839436079602713 1104 90-91 0.935418839513094 1104 92-93 0.8815540617171536 1104 94-95 1.0534692813499547 1104 96-97 1.1433496257414113 1104 98-99 1.2392944728724977 1104 100-101 1.4362843814816202 1105 1 0.24357309218890677 1105 2 0.2830829485167641 1105 3 0.2282092482625373 1105 4 0.27404362589614095 1105 5 0.16837368101576544 1105 6 0.20834616883085744 1105 7 0.17443047511087428 1105 8 0.18551604955491285 1105 9 0.22897184997069786 1105 10-11 0.2811832426686962 1105 12-13 0.2213160132954144 1105 14-15 0.3339921953182454 1105 16-17 0.3269377138036944 1105 18-19 0.35442260058439956 1105 20-21 0.4074186360629639 1105 22-23 0.46211632081907794 1105 24-25 0.346798202650767 1105 26-27 0.3264539924606993 1105 28-29 0.4090226491800948 1105 30-31 0.4118661904186567 1105 32-33 0.3207120057870654 1105 34-35 0.489570434905076 1105 36-37 0.5916464543105988 1105 38-39 0.6902897395000238 1105 40-41 0.6890810808815147 1105 42-43 0.5343813469643592 1105 44-45 0.5778451764763517 1105 46-47 0.48891567209168585 1105 48-49 0.48278800785906384 1105 50-51 0.3959553823589559 1105 52-53 0.4615532319472848 1105 54-55 0.5320351523728348 1105 56-57 0.5362902643445864 1105 58-59 0.5540805841763401 1105 60-61 0.6858231997608115 1105 62-63 0.5736727529389682 1105 64-65 0.6243777794631029 1105 66-67 0.7295580274107394 1105 68-69 0.606169520105567 1105 70-71 0.510938646184421 1105 72-73 0.6813436471540726 1105 74-75 0.5998227936500165 1105 76-77 0.47485683714558036 1105 78-79 0.6331028576130429 1105 80-81 0.5371838426850566 1105 82-83 0.5379930570685865 1105 84-85 0.4648798786920203 1105 86-87 0.59261911972661 1105 88-89 0.7010178994198988 1105 90-91 0.745104666856502 1105 92-93 0.9011030651731744 1105 94-95 1.0376040062088627 1105 96-97 1.2090957473659394 1105 98-99 1.2789064858863917 1105 100-101 1.5651705783159606 1106 1 0.2325162628946238 1106 2 0.24036162794966032 1106 3 0.18755116660077675 1106 4 0.2817093134883919 1106 5 0.3049474975161459 1106 6 0.21051420343463434 1106 7 0.2963614477150429 1106 8 0.29992498709726334 1106 9 0.2864761345251665 1106 10-11 0.28719412964654367 1106 12-13 0.11372541947471149 1106 14-15 0.2814795418263998 1106 16-17 0.21268744165869435 1106 18-19 0.3767763178585568 1106 20-21 0.32524821117528546 1106 22-23 0.4620904162644024 1106 24-25 0.3504475067896635 1106 26-27 0.39012915405508153 1106 28-29 0.4696041298962186 1106 30-31 0.49068109236697666 1106 32-33 0.5383417625845084 1106 34-35 0.7082334301898641 1106 36-37 0.47472433341050646 1106 38-39 0.5315238148222221 1106 40-41 0.5529164385969167 1106 42-43 0.6969960114150098 1106 44-45 0.6455242108016606 1106 46-47 0.5991026087780185 1106 48-49 0.7689108790492512 1106 50-51 0.8027446128935978 1106 52-53 0.8466347400398107 1106 54-55 0.7436593208513571 1106 56-57 0.8223439585990064 1106 58-59 0.7205439883998466 1106 60-61 0.8144026646315936 1106 62-63 0.7618777321476173 1106 64-65 0.806620148363983 1106 66-67 0.8579005244802858 1106 68-69 0.6576189093762537 1106 70-71 0.6298570368286462 1106 72-73 0.8732896267377512 1106 74-75 0.8164658224105459 1106 76-77 0.8157023441477556 1106 78-79 0.9244287174823533 1106 80-81 0.9193053138518188 1106 82-83 0.8811350911530944 1106 84-85 0.8837989169501022 1106 86-87 1.1425293957690812 1106 88-89 1.2947223272736537 1106 90-91 1.1851113372793272 1106 92-93 1.20668364217358 1106 94-95 1.5119183155904956 1106 96-97 1.8766350737179955 1106 98-99 1.6383717746662434 1106 100-101 1.8528187273472163 1107 1 0.2815321122033936 1107 2 0.2838056262378075 1107 3 0.26873020395478164 1107 4 0.14264731994845192 1107 5 0.21536891887560472 1107 6 0.13019575299691866 1107 7 0.20074112295496604 1107 8 0.20396355576750835 1107 9 0.3039098417112953 1107 10-11 0.313210577306144 1107 12-13 0.23189488521196466 1107 14-15 0.22648584148235784 1107 16-17 0.41673140147666743 1107 18-19 0.519514441858945 1107 20-21 0.33438620658681373 1107 22-23 0.36122470442990817 1107 24-25 0.4244370160155171 1107 26-27 0.5205486718734846 1107 28-29 0.4947160627691076 1107 30-31 0.6339801286826798 1107 32-33 0.5268336588907729 1107 34-35 0.641724304840352 1107 36-37 0.6933325453930976 1107 38-39 0.556446059163676 1107 40-41 0.6904456156881409 1107 42-43 0.5547312767759465 1107 44-45 0.6969206511798873 1107 46-47 0.5558987162362357 1107 48-49 0.6026332402654688 1107 50-51 0.530577844061348 1107 52-53 0.656387459407938 1107 54-55 0.7119485565929295 1107 56-57 0.6095883033848679 1107 58-59 0.6931281718373086 1107 60-61 0.8080852045676821 1107 62-63 0.7126454049621955 1107 64-65 0.8886251627969486 1107 66-67 0.9229775014174848 1107 68-69 1.0102715204555324 1107 70-71 1.0205708181330735 1107 72-73 0.9931594671265032 1107 74-75 0.8022059038634701 1107 76-77 0.9059423367465804 1107 78-79 1.0502248269372991 1107 80-81 1.3008686202360664 1107 82-83 1.3243193825466264 1107 84-85 1.269271970196673 1107 86-87 1.3151085208457012 1107 88-89 1.3405504108936803 1107 90-91 1.522295514194166 1107 92-93 1.3702472374168586 1107 94-95 1.7084737382531507 1107 96-97 1.794498442067166 1107 98-99 1.9945743899719126 1107 100-101 1.5568295748689245 1108 1 0.1339544886604358 1108 2 0.14344575528638615 1108 3 0.2320546156889023 1108 4 0.27409254616430445 1108 5 0.22668517598987137 1108 6 0.18847194371912224 1108 7 0.27665684530313683 1108 8 0.1858083994209494 1108 9 0.21866021907441535 1108 10-11 0.37316975122037377 1108 12-13 0.4124539456026497 1108 14-15 0.32639036646185104 1108 16-17 0.5583185718593384 1108 18-19 0.45591824257942903 1108 20-21 0.46882367889309506 1108 22-23 0.5319756355017802 1108 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-1.7466035656136576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7427.0 3 1372.0 4 1274.0 5 1524.0 6 1789.0 7 2125.0 8 2634.0 9 3286.0 10 4257.0 11 5561.0 12 7292.0 13 9312.0 14 10269.0 15 13091.0 16 16529.0 17 20966.0 18 18804.0 19 14318.0 20 14361.0 21 15819.0 22 17260.0 23 19568.0 24 22187.0 25 25275.0 26 29525.0 27 34632.0 28 41262.0 29 49855.0 30 60952.0 31 75407.0 32 95913.0 33 125184.0 34 167729.0 35 234369.0 36 333793.0 37 427992.0 38 267105.0 39 20915.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.917248455137106 18.70273062757289 14.822645585602833 23.557375331687165 2 15.349556147839968 20.102819129341913 39.58933743598633 24.958287286831784 3 18.71112373098423 23.686293027133882 30.336885907870208 27.26569733401168 4 13.095484237945405 16.349740195835007 36.462148948368714 34.09262661785087 5 14.14086643626111 36.50129687778648 34.39008368191392 14.967753004038492 6 32.75340536649132 35.26407095900569 17.12367757027473 14.858846104228258 7 28.542160033560226 32.46627061991691 20.8919622357245 18.099607110798363 8 26.401693891534467 34.1656641437823 20.201163152085318 19.231478812597913 9 26.091965377549176 16.639173853364746 20.68285021310877 36.5860105559773 10-11 24.847119908695987 26.15097642064806 27.424157670845418 21.577745999810535 12-13 25.55230787967361 23.655214098521856 27.92478021804535 22.867697803759185 14-15 22.916074642417353 25.02956738250856 25.93041403356834 26.12394394150575 16-17 22.379746778342522 28.228339308450234 26.740946823903023 22.65096708930422 18-19 22.344810010103927 26.891125685623557 28.92778850317552 21.836275801096996 20-21 22.67665529381227 25.988787714160004 29.170345423108294 22.16421156891943 22-23 22.91839140364404 25.657410913365077 28.958306600145097 22.465891082845786 24-25 22.743343293181965 25.982823715189674 28.84425988642209 22.429573105206266 26-27 22.36988689974688 26.143744125335793 28.883818310730724 22.602550664186605 28-29 22.432476016595267 26.29061560130768 28.920033515306866 22.35687486679019 30-31 22.437418154046995 26.039564835527695 28.8087830434357 22.714233966989607 32-33 22.367654465620713 26.132873443648812 28.846514298886643 22.652957791843832 34-35 22.912167895384762 25.95936321081338 28.62610175256416 22.5023671412377 36-37 22.729982866263494 25.78746860431081 28.61390371823279 22.868644811192897 38-39 22.445532357909247 26.197029892515744 28.680034157205675 22.677403592369334 40-41 22.787623191700085 26.089377235851558 28.62735626958484 22.495643302863513 42-43 22.541488072390614 26.089460791499462 28.33166372210693 23.037387414002993 44-45 22.677236565384334 26.217310652405573 28.296460850591178 22.80899193161892 46-47 22.782030076171498 26.500887923206935 27.837779565001075 22.879302435620495 48-49 22.81021259896683 26.562522905725956 27.606922519161873 23.020341976145342 50-51 22.821734547093186 26.71065576321669 27.644589571173974 22.82302011851615 52-53 22.91950012734359 26.710143204578323 27.356335789143376 23.01402087893471 54-55 22.840304660977388 26.60271979425867 27.46707151017544 23.0899040345885 56-57 22.846742008607823 27.066320646035464 27.394024041203185 22.692913304153535 58-59 22.969124385930968 26.442185623490055 27.64107746932079 22.947612521258186 60-61 22.84437371449341 26.444919551695573 27.834698452454127 22.876008281356892 62-63 22.538639400620827 26.667872304058744 28.08302301700084 22.71046527831959 64-65 22.823453170639972 26.649516127504764 27.65232434226577 22.874706359589485 66-67 22.637843034860197 26.749390976989257 27.491154397367108 23.121611590783438 68-69 22.517550893758347 26.630250948690865 27.7819170095063 23.070281148044483 70-71 22.673010199215156 26.564861673243133 27.697483941044744 23.06464418649697 72-73 22.708447107325835 26.457566040024695 27.66012425811089 23.173862594538576 74-75 22.66270286700511 26.997124110614184 27.212601599800845 23.127571422579855 76-77 22.85543616476005 26.651795871730826 27.429242895087608 23.063525068421516 78-79 22.779059512295444 26.62397742804253 27.43702882538371 23.15993423427832 80-81 22.63528170252682 26.855791215877982 27.29558358625604 23.213343495339156 82-83 22.768468070109137 26.686407250560727 27.405889299249107 23.13923538008103 84-85 22.66470653888257 26.75355714715727 27.25375049181695 23.327985822143205 86-87 22.69045097580767 26.680283339221766 27.48682204764603 23.142443637324533 88-89 22.731610000883855 26.479303415820244 27.35995443648977 23.429132146806133 90-91 22.615930506186057 26.392234890678566 27.537243968853698 23.454590634281676 92-93 22.658011508144085 26.379038222190154 27.523561932935298 23.43938833673046 94-95 22.633757568268877 26.22075399033679 27.541396051918348 23.60409238947599 96-97 22.612835654646346 26.155887611922463 27.508765521455516 23.722511211975675 98-99 22.37754874024975 26.215752509590434 27.431702318458168 23.97499643170164 100-101 22.69606579973447 25.614313692203677 28.046566437635505 23.64305407042635 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2511.0 1 1440.0 2 369.5 3 363.0 4 336.5 5 341.5 6 549.0 7 1075.0 8 1782.5 9 2240.0 10 2256.5 11 2243.0 12 2432.0 13 2618.0 14 2766.0 15 2829.0 16 2852.0 17 2980.5 18 3161.5 19 3322.5 20 3600.0 21 4099.0 22 4788.0 23 5589.0 24 6680.5 25 8325.5 26 10533.5 27 12753.0 28 13974.0 29 15665.0 30 18872.5 31 22522.0 32 26603.5 33 30243.0 34 34227.0 35 38421.0 36 44315.5 37 51120.5 38 55352.0 39 57609.5 40 59434.5 41 64468.0 42 69366.5 43 73693.0 44 79467.5 45 83225.5 46 86248.5 47 91139.5 48 96822.0 49 98638.0 50 96105.5 51 91084.0 52 86328.5 53 86247.0 54 85244.5 55 78108.5 56 69538.0 57 60914.0 58 53420.5 59 47259.5 60 39483.5 61 31321.5 62 24169.0 63 18270.5 64 13812.5 65 10292.0 66 7308.0 67 5213.0 68 3760.0 69 2594.5 70 1802.5 71 1173.0 72 728.5 73 508.0 74 367.5 75 287.5 76 191.5 77 109.0 78 62.5 79 48.0 80 38.0 81 23.5 82 20.5 83 17.5 84 12.0 85 9.5 86 9.5 87 9.0 88 7.5 89 8.0 90 7.0 91 3.0 92 1.0 93 1.0 94 1.5 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.292624502743214 2 0.2603858284911535 3 0.2581795505046298 4 0.26137640228102127 5 0.27015648814575843 6 0.17978914286100223 7 0.18122997746444627 8 0.18136505570851916 9 0.1797441167796446 10-11 0.187533628854514 12-13 0.17852841258298868 14-15 0.17852841258298868 16-17 0.18471949876966232 18-19 0.17897867339656495 20-21 0.17170696125730828 22-23 0.1715268569318778 24-25 0.1740933435692625 26-27 0.17051377010133117 28-29 0.18179280348141663 30-31 0.18541740303070553 32-33 0.18782629838333856 34-35 0.18577761168156653 36-37 0.16533577074520417 38-39 0.15941484104667628 40-41 0.16475043168755502 42-43 0.17344046538957691 44-45 0.17774045615923023 46-47 0.17848338650163106 48-49 0.17864097778638277 50-51 0.18109489922037342 52-53 0.20218961833642135 54-55 0.17028863969454305 56-57 0.1590996584771729 58-59 0.16002269314500425 60-61 0.1541693025685128 62-63 0.16092321477215676 64-65 0.15813159772798394 66-67 0.15529495460245346 68-69 0.16520069250113129 70-71 0.16022531051111355 72-73 0.1555200850092416 74-75 0.1591221715178517 76-77 0.16270174498578302 78-79 0.16526823162316773 80-81 0.15871693678563306 82-83 0.157883954280517 84-85 0.15275098100574758 86-87 0.14905884233442224 88-89 0.15178292025655862 90-91 0.1588970411110636 92-93 0.15196302458198913 94-95 0.155475058927884 96-97 0.1548897198702348 98-99 0.15720856306015257 100-101 0.14023373038832745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2220935.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.47691054518207 #Duplication Level Percentage of deduplicated Percentage of total 1 65.3402231691458 25.14089921881456 2 14.979031960303107 11.52693745580012 3 6.76710456979259 7.811318315454069 4 3.661353370058687 5.635102643761961 5 2.249230777318633 4.327172570717969 6 1.4506807123100085 3.3490627198303926 7 1.0147513740800878 2.7331148492256094 8 0.7426080194714862 2.2858609868271365 9 0.5669912468310259 1.9634464335796769 >10 2.8956969105065573 19.950411032045455 >50 0.21654359361549455 5.724742981534554 >100 0.11132376998620999 7.571442285799695 >500 0.003521468352869536 0.8488775708485827 >1k 7.042936705739051E-4 0.5119461168166355 >5k 2.347645568579684E-4 0.6196648189434705 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7562 0.34048722722637087 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6158 0.2772706090002634 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3585 0.16141850166709065 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2754 0.12400182805890313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1256520339406601E-4 0.0 18-19 0.0 0.0 0.0 1.8010432543050562E-4 0.0 20-21 0.0 0.0 0.0 1.8010432543050562E-4 0.0 22-23 0.0 0.0 0.0 3.37695610182198E-4 0.0 24-25 0.0 0.0 0.0 4.727738542550773E-4 0.0 26-27 0.0 0.0 0.0 8.780085864737149E-4 0.0 28-29 0.0 0.0 0.0 0.002206277986523694 0.0 30-31 0.0 0.0 0.0 0.004637686379835519 0.0 32-33 0.0 0.0 0.0 0.00907275539356172 0.0 34-35 0.0 0.0 0.0 0.013980598261543 0.0 36-37 0.0 0.0 0.0 0.01963137147192511 0.0 38-39 0.0 0.0 0.0 0.02483188386873096 0.0 40-41 0.0 0.0 0.0 0.032441291618169825 0.0 42-43 0.0 0.0 0.0 0.04164912525580443 0.0 44-45 0.0 0.0 0.0 0.04950617645271023 0.0 46-47 0.0 0.0 0.0 0.05945694043274567 0.0 48-49 0.0 0.0 0.0 0.06967786090092687 0.0 50-51 0.0 0.0 0.0 0.08446892862690714 0.0 52-53 0.0 0.0 0.0 0.12064738499775995 0.0 54-55 0.0 0.0 0.0 0.1706938744267617 0.0 56-57 0.0 0.0 0.0 0.21477440807587794 0.0 58-59 0.0 0.0 0.0 0.27634757433243207 0.0 60-61 0.0 0.0 0.0 0.3464531830062564 0.0 62-63 0.0 0.0 0.0 0.39575674209285727 0.0 64-65 0.0 0.0 0.0 0.4445199882031667 0.0 66-67 0.0 0.0 0.0 0.5610699997973827 0.0 68-69 0.0 0.0 0.0 0.6804791675578079 0.0 70-71 0.0 0.0 0.0 0.7463072985026576 0.0 72-73 0.0 0.0 0.0 0.8712771873107497 0.0 74-75 0.0 0.0 0.0 1.0473741915004267 0.0 76-77 0.0 0.0 0.0 1.2265554822631008 0.0 78-79 0.0 0.0 0.0 1.4124456591480614 0.0 80-81 0.0 0.0 0.0 1.649327873170534 0.0 82-83 0.0 0.0 0.0 1.896746190230691 0.0 84-85 0.0 0.0 0.0 2.1707749213732055 0.0 86-87 0.0 4.5026081357626405E-5 0.0 2.5284395986375108 0.0 88-89 0.0 4.5026081357626405E-5 0.0 2.9377491912190137 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5820 0.0 39.302486 1 GTATCAA 8840 0.0 33.568367 1 ATCAACG 11055 0.0 26.749268 3 TCAACGC 11120 0.0 26.550755 4 CAACGCA 11290 0.0 26.152147 5 TATCAAC 11495 0.0 25.76615 2 AACGCAG 11550 0.0 25.540373 6 GCGTACA 145 2.3199958E-5 22.950941 2 GAGTACT 6970 0.0 22.52456 12-13 GTACATG 6850 0.0 22.35441 1 CGCAGAG 12990 0.0 22.16008 8 ACGCAGA 13070 0.0 22.06178 7 ACCGTAT 110 0.0028776538 21.591616 8 TACATGG 7000 0.0 21.461472 2 AGAGTAC 10795 0.0 21.055813 10-11 GTACTTT 7495 0.0 20.850544 14-15 GCAGAGT 13575 0.0 20.645245 9 CCCTATA 255 1.1814336E-8 20.507984 2 ACATGGG 7080 0.0 20.413647 3 AGTACTT 7425 0.0 20.408537 12-13 >>END_MODULE