FastQCFastQC Report
Thu 26 May 2016
SRR937767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220935
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93340.42027344339208483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81930.36889868456303315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51950.23391049265286917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66250.037.9447671
GTATCAA101900.032.9706341
GTATACG600.00394813631.6725674
TATCAAC126900.026.3938052
ATCAACG126000.026.3183943
TCAACGC126500.026.2143694
CAACGCA128950.025.7163075
AACGCAG131700.025.2097286
TATACGT1252.2539785E-422.8042475
GAGTACT89700.022.79747612-13
GGACCGA6250.022.0391456
GTACATG67800.022.0080761
ACGCAGA152200.021.7532527
CGCAGAG151800.021.6854048
GTACTTT94500.021.41186714-15
TACATGG69700.020.9938682
CTATACG1150.00372033720.6560234
AGAGTAC128150.020.10884910-11
AGTACTT93800.020.0032412-13
GCAGAGT161850.019.9573219
Produced by FastQC (version 0.11.5)