##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937767_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2220935 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12528822320329 33.0 31.0 34.0 31.0 34.0 2 32.40814656890004 34.0 31.0 34.0 31.0 34.0 3 32.50408724253524 34.0 31.0 34.0 31.0 34.0 4 36.14732488794134 37.0 35.0 37.0 35.0 37.0 5 36.05262288180428 37.0 35.0 37.0 35.0 37.0 6 36.08479266615187 37.0 35.0 37.0 35.0 37.0 7 36.0275172393609 37.0 35.0 37.0 35.0 37.0 8 35.99922149905333 37.0 35.0 37.0 35.0 37.0 9 37.69678896500798 39.0 37.0 39.0 35.0 39.0 10-11 37.61326310765511 39.0 37.0 39.0 35.0 39.0 12-13 37.50856373554382 39.0 37.0 39.0 35.0 39.0 14-15 38.74771256250183 40.0 38.0 41.0 35.0 41.0 16-17 38.70077850094667 40.0 38.0 41.0 34.5 41.0 18-19 38.663027058423594 40.0 38.0 41.0 34.0 41.0 20-21 38.55139119334875 40.0 38.0 41.0 34.0 41.0 22-23 38.48949856704496 40.0 38.0 41.0 34.0 41.0 24-25 38.510331009237106 40.0 38.0 41.0 34.0 41.0 26-27 38.37515235700279 40.0 38.0 41.0 34.0 41.0 28-29 38.22203261239073 40.0 38.0 41.0 34.0 41.0 30-31 38.29152699201012 40.0 38.0 41.0 34.0 41.0 32-33 38.22899431995984 40.0 38.0 41.0 34.0 41.0 34-35 38.090016592110985 40.0 37.5 41.0 33.5 41.0 36-37 37.95520985530869 40.0 37.0 41.0 33.0 41.0 38-39 37.83247168422308 40.0 37.0 41.0 33.0 41.0 40-41 37.66391407222633 40.0 37.0 41.0 33.0 41.0 42-43 37.44499366257905 40.0 37.0 41.0 33.0 41.0 44-45 37.15857555489017 40.0 36.5 41.0 31.5 41.0 46-47 36.808416049996964 39.5 36.0 41.0 31.0 41.0 48-49 36.49570338618645 39.0 36.0 41.0 30.5 41.0 50-51 36.23021520215585 39.0 35.5 41.0 30.0 41.0 52-53 35.982184305258826 39.0 35.0 40.0 29.0 41.0 54-55 35.744908788415685 39.0 35.0 40.0 28.5 41.0 56-57 35.44649168030582 38.0 35.0 40.0 28.0 41.0 58-59 35.17234408030852 38.0 34.0 40.0 28.0 41.0 60-61 34.82558967281798 38.0 34.0 40.0 27.0 41.0 62-63 34.433399446629466 37.0 34.0 40.0 26.0 41.0 64-65 33.99883877736178 36.5 33.0 39.0 26.0 41.0 66-67 33.55045375033488 36.0 33.0 39.0 25.5 40.0 68-69 33.08118292520943 35.5 32.0 38.5 24.5 40.0 70-71 32.66732952562772 35.0 32.0 38.0 24.0 40.0 72-73 32.17387586759631 35.0 31.5 37.0 22.5 39.0 74-75 31.692542555275143 35.0 31.0 36.5 21.5 39.0 76-77 30.458438675602842 33.5 29.5 35.0 20.0 37.5 78-79 31.06833068054671 34.0 31.0 36.0 20.5 37.0 80-81 30.903852206390553 34.0 31.0 35.0 20.0 37.0 82-83 30.55911159038873 34.0 31.0 35.0 18.5 36.5 84-85 30.207474554635773 34.0 30.0 35.0 18.0 36.0 86-87 29.866963013325467 34.0 30.0 35.0 14.0 36.0 88-89 29.462513535965705 34.0 29.5 35.0 7.5 35.0 90-91 28.945648566932398 34.0 29.0 35.0 4.0 35.0 92-93 28.50972540844284 33.5 29.0 35.0 2.0 35.0 94-95 28.04346570250818 33.0 28.0 35.0 2.0 35.0 96-97 27.48142043778859 33.0 27.0 35.0 2.0 35.0 98-99 26.59171632668223 32.5 25.5 34.0 2.0 35.0 100-101 23.833757854237067 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3372258650947515 1101 2 0.22372081768143914 1101 3 0.2132293025914649 1101 4 0.06827912259867475 1101 5 0.036763018478943366 1101 6 0.0342684402876543 1101 7 0.11870842517106439 1101 8 0.06971514415361924 1101 9 0.08006099796843102 1101 10-11 0.1796646174888039 1101 12-13 0.15945292090095364 1101 14-15 0.1300626321017404 1101 16-17 0.1657090821371625 1101 18-19 0.234458131594792 1101 20-21 0.175904027184977 1101 22-23 0.09258247134957998 1101 24-25 0.13538051100709225 1101 26-27 0.07176772500378092 1101 28-29 0.00369891957385704 1101 30-31 0.07297910827107046 1101 32-33 0.18759202518202045 1101 34-35 0.11421389129615278 1101 36-37 0.060798801951079895 1101 38-39 0.13646104367025913 1101 40-41 0.24839835026033086 1101 42-43 0.25381199057522963 1101 44-45 -0.05478206515061146 1101 46-47 -0.03832554541129696 1101 48-49 -0.18943188008589829 1101 50-51 -0.038561605509961794 1101 52-53 0.13328740787310522 1101 54-55 -0.09979456469376657 1101 56-57 -0.15737749320101813 1101 58-59 -0.11144887994566233 1101 60-61 0.01888520147647199 1101 62-63 -0.025653134414731937 1101 64-65 -0.03945775948882613 1101 66-67 0.03275125121003697 1101 68-69 0.12606779151469993 1101 70-71 0.025800451543929626 1101 72-73 -0.17541837657860526 1101 74-75 -0.17372699373378708 1101 76-77 0.0068697823496641774 1101 78-79 0.023376244152991887 1101 80-81 0.06933810735526436 1101 82-83 0.029273429772867843 1101 84-85 -0.11248788054795966 1101 86-87 -0.04780987238413559 1101 88-89 -0.11731161864161166 1101 90-91 -0.27157851955055534 1101 92-93 -0.4114928566894811 1101 94-95 -0.6137173646901637 1101 96-97 -0.5562983144099576 1101 98-99 -0.5982843849341428 1101 100-101 -0.556201698807584 1102 1 0.2469897645616257 1102 2 0.22398718751368563 1102 3 0.19028009642824628 1102 4 0.13251174704455337 1102 5 0.052578824911655886 1102 6 0.06575241708770108 1102 7 0.0790661887580697 1102 8 0.11795932951889654 1102 9 0.159807907570638 1102 10-11 0.1544282240269652 1102 12-13 0.2456020801955816 1102 14-15 0.22230041209274987 1102 16-17 0.24572587671602975 1102 18-19 0.2699649958906818 1102 20-21 0.2694541277571645 1102 22-23 0.24073916867611445 1102 24-25 0.2091481599613374 1102 26-27 0.23978576941176755 1102 28-29 0.12331498768953963 1102 30-31 0.22854065259117817 1102 32-33 0.2725393956587183 1102 34-35 0.1368666536138008 1102 36-37 0.0739653612105613 1102 38-39 0.2072216818642474 1102 40-41 0.26417197496007816 1102 42-43 0.2183769914540079 1102 44-45 0.20481838010100972 1102 46-47 0.16306723291978642 1102 48-49 0.10586173211556371 1102 50-51 0.22429698804170073 1102 52-53 0.1236742690444359 1102 54-55 0.13702735418451084 1102 56-57 0.13286056019011738 1102 58-59 0.07517521441643993 1102 60-61 -0.0482446831096226 1102 62-63 -0.18869998347833672 1102 64-65 -0.183583367315876 1102 66-67 -0.06354144314815358 1102 68-69 -0.09037292948048048 1102 70-71 -0.02117007982873531 1102 72-73 0.02905366825901723 1102 74-75 -0.13855445872880878 1102 76-77 -0.1974636095694109 1102 78-79 -0.05048357969921824 1102 80-81 -0.054964819978884805 1102 82-83 -0.1919728842076438 1102 84-85 -0.19093106214423017 1102 86-87 -0.3531056327293989 1102 88-89 -0.3418715420895744 1102 90-91 -0.18955887379899394 1102 92-93 -0.28892016155014844 1102 94-95 -0.552062122242841 1102 96-97 -0.49851754224989975 1102 98-99 -0.41013549903963664 1102 100-101 -0.41214209758119225 1103 1 0.24398647062633927 1103 2 0.2209684920710444 1103 3 0.22440486851078134 1103 4 0.16611088058556334 1103 5 0.1509398064348204 1103 6 0.1241047538938318 1103 7 0.0961857094234162 1103 8 0.15788649529376642 1103 9 0.15820316986824423 1103 10-11 0.15511880774409548 1103 12-13 0.2004058600235865 1103 14-15 0.2833306238883182 1103 16-17 0.22146105028108565 1103 18-19 0.15261867560888476 1103 20-21 0.030694992427591217 1103 22-23 0.0935042360701317 1103 24-25 0.13598329924560915 1103 26-27 0.040664400565546543 1103 28-29 -0.03534065944701581 1103 30-31 0.07034108880161938 1103 32-33 0.15021391709413479 1103 34-35 0.18922482311980815 1103 36-37 0.1725769898454388 1103 38-39 0.19905192744376876 1103 40-41 0.21183169107552402 1103 42-43 0.21083820201249637 1103 44-45 0.309850201602508 1103 46-47 0.14463460954626584 1103 48-49 0.111537435989284 1103 50-51 0.029089340944885578 1103 52-53 0.23506368373747222 1103 54-55 0.02238722779279101 1103 56-57 0.18782630425681646 1103 58-59 0.10069774781535301 1103 60-61 0.055363741018084056 1103 62-63 -0.0052166096539352225 1103 64-65 -0.008464750868057536 1103 66-67 0.041402937537363016 1103 68-69 0.16489413762538163 1103 70-71 0.14542901893467075 1103 72-73 0.17961358757498402 1103 74-75 0.19305638989424878 1103 76-77 0.18807785277887135 1103 78-79 0.06808889929759943 1103 80-81 6.535008593502312E-4 1103 82-83 0.0724260347059058 1103 84-85 0.05090613895474405 1103 86-87 -0.029831719405109425 1103 88-89 0.07731873608151929 1103 90-91 0.0628450034062773 1103 92-93 -0.029406998229578818 1103 94-95 -0.3051041683579321 1103 96-97 -0.37490951558044827 1103 98-99 -0.09137472419476822 1103 100-101 -0.007915625867799037 1104 1 0.2968848850870529 1104 2 0.2036729282255294 1104 3 0.17419293404625336 1104 4 0.13689984430428837 1104 5 0.11638733853276761 1104 6 0.09560854837088328 1104 7 0.1254140112415172 1104 8 0.04118967023832454 1104 9 0.11753870193015814 1104 10-11 0.19560499024758116 1104 12-13 0.17059381415881347 1104 14-15 0.1485714199496755 1104 16-17 0.23313593121456222 1104 18-19 0.30080361728327887 1104 20-21 0.06512810292290538 1104 22-23 0.14514207867374296 1104 24-25 0.07443794737209686 1104 26-27 0.1952539180575954 1104 28-29 0.08929127496496392 1104 30-31 0.10702594433598733 1104 32-33 0.22660068688860946 1104 34-35 0.24754743117673428 1104 36-37 0.14060919157297036 1104 38-39 0.006647600099704221 1104 40-41 -0.0387723212100326 1104 42-43 0.07774512237745057 1104 44-45 -0.07968564446697712 1104 46-47 -0.11829155363482613 1104 48-49 -0.1850045397210991 1104 50-51 -0.11338511825909592 1104 52-53 -0.009074666510464624 1104 54-55 -0.12424456624286506 1104 56-57 -0.005119916974017258 1104 58-59 -0.1191609412724901 1104 60-61 -0.1619737105211172 1104 62-63 -0.2960490412383123 1104 64-65 -0.264749500106781 1104 66-67 -0.2364320166920777 1104 68-69 -0.05864201697173854 1104 70-71 0.01785434267286945 1104 72-73 -0.03022170671045643 1104 74-75 0.03987316914347261 1104 76-77 -0.0800000087868078 1104 78-79 -0.09456831561367096 1104 80-81 -0.09529840636316322 1104 82-83 -0.10226394569020414 1104 84-85 0.0115978601673703 1104 86-87 -0.20327126206355928 1104 88-89 -0.29103942772113456 1104 90-91 -0.285208513752476 1104 92-93 -0.16365527364642318 1104 94-95 -0.21806512534910638 1104 96-97 -0.34376305015487674 1104 98-99 -0.1752507959248213 1104 100-101 -0.22942627025891582 1105 1 0.20580872338058498 1105 2 0.13477611368723075 1105 3 0.13726018186986977 1105 4 0.036915605294566944 1105 5 -0.012083008980944498 1105 6 0.05254169618467586 1105 7 0.06800659308309065 1105 8 0.09533522997172383 1105 9 0.1049453911696574 1105 10-11 0.15496614764181516 1105 12-13 0.18344998727426542 1105 14-15 0.13319123067587668 1105 16-17 0.0012652091784346453 1105 18-19 0.1464150415715011 1105 20-21 0.14335572473568448 1105 22-23 0.14364338696494627 1105 24-25 0.05209808752665168 1105 26-27 0.19322606131360232 1105 28-29 0.16838757891598277 1105 30-31 0.1104279960169876 1105 32-33 0.11253928181244532 1105 34-35 0.20346643559819455 1105 36-37 0.19210021780695996 1105 38-39 0.1766878282534705 1105 40-41 0.1178154824497426 1105 42-43 0.23313687544467143 1105 44-45 0.1490246012303018 1105 46-47 0.10699965377528287 1105 48-49 0.18921598470058854 1105 50-51 0.1311086948534026 1105 52-53 0.21975738820447788 1105 54-55 0.2595498459377765 1105 56-57 0.3108742843576877 1105 58-59 0.3095195026068822 1105 60-61 0.23945849690124987 1105 62-63 -0.02587448988361274 1105 64-65 0.1495202811723857 1105 66-67 0.062259821114651004 1105 68-69 0.14521370610616202 1105 70-71 -0.07182569202280575 1105 72-73 -0.00563187411883348 1105 74-75 -0.061770274252314294 1105 76-77 -0.08859071608113211 1105 78-79 0.01797246567990385 1105 80-81 0.09964751924883686 1105 82-83 -0.06340117102054421 1105 84-85 0.013084295717284533 1105 86-87 -0.07813985776362742 1105 88-89 -0.0877928572416593 1105 90-91 0.05068065874823091 1105 92-93 0.18360503405197193 1105 94-95 0.05655518637447088 1105 96-97 -0.06805968102280957 1105 98-99 0.1768954187605125 1105 100-101 0.06572106489735674 1106 1 0.23201942278589627 1106 2 0.16835695326562217 1106 3 0.2489715132874437 1106 4 0.19580266320243567 1106 5 0.16700018173015252 1106 6 0.11619095322438255 1106 7 0.05140883841924193 1106 8 0.07305554673732928 1106 9 0.14718777184533138 1106 10-11 0.07134536206264386 1106 12-13 -0.16330190537726708 1106 14-15 0.08577927230921034 1106 16-17 0.1913373769127915 1106 18-19 0.22131114370138505 1106 20-21 0.23828414279751797 1106 22-23 0.2757030404444336 1106 24-25 0.22140363351517323 1106 26-27 0.16452558096073489 1106 28-29 0.19038687478308702 1106 30-31 0.34981345755667803 1106 32-33 0.14567944959153323 1106 34-35 -0.22397181589146697 1106 36-37 0.11828901608877374 1106 38-39 0.07666960675422985 1106 40-41 -0.03395474061790793 1106 42-43 -0.07951108224612824 1106 44-45 0.02559543281949317 1106 46-47 0.167314140956357 1106 48-49 -0.10826618089664208 1106 50-51 0.12748720867378438 1106 52-53 0.17793566217222434 1106 54-55 0.2662020178286326 1106 56-57 0.23932767058341398 1106 58-59 0.304307388459641 1106 60-61 0.1980934858299932 1106 62-63 0.08432068916102509 1106 64-65 -0.07291256274147884 1106 66-67 -0.24164505500564815 1106 68-69 0.17866325669157135 1106 70-71 0.1717083250730198 1106 72-73 0.21626212562093627 1106 74-75 -0.027440410363087864 1106 76-77 -0.032220344533282486 1106 78-79 -0.07548689919929075 1106 80-81 -0.14696192778900752 1106 82-83 -0.15642236624492156 1106 84-85 -0.310135516792613 1106 86-87 -0.22033407951721173 1106 88-89 -0.2504737549286773 1106 90-91 -0.2178480155331961 1106 92-93 -0.4014933414380799 1106 94-95 -0.25106566867600577 1106 96-97 -0.11370733315760617 1106 98-99 -0.18259077028504223 1106 100-101 -0.5416827511967917 1107 1 0.3028311499299292 1107 2 0.2655250698848022 1107 3 0.22045600242294938 1107 4 0.08507214937280594 1107 5 0.11300850273373442 1107 6 0.14438532473303667 1107 7 0.10915135853454672 1107 8 0.1583980809627974 1107 9 0.1708346104030838 1107 10-11 0.19110238769322763 1107 12-13 0.30984718381827037 1107 14-15 0.27294344873152454 1107 16-17 0.2684276280537432 1107 18-19 0.2832459467547679 1107 20-21 0.33182787731876573 1107 22-23 0.3238506487382793 1107 24-25 0.19048223977703316 1107 26-27 0.23696297209782102 1107 28-29 0.3079110667265752 1107 30-31 0.25432325247341936 1107 32-33 0.1847466346070732 1107 34-35 0.19325792620534799 1107 36-37 0.3000169521510898 1107 38-39 0.3341523201388483 1107 40-41 0.20291215726909684 1107 42-43 0.22166505320612373 1107 44-45 0.20602433522840613 1107 46-47 0.15030656739214976 1107 48-49 0.041968633538061795 1107 50-51 0.1933186135070457 1107 52-53 0.25652528467873736 1107 54-55 0.24785714011783 1107 56-57 0.31810498489998196 1107 58-59 0.3972424557458183 1107 60-61 0.4230472783693173 1107 62-63 0.2659782407945741 1107 64-65 0.3596653110564034 1107 66-67 0.13894801117967148 1107 68-69 0.1712324580629172 1107 70-71 0.35979848103692547 1107 72-73 0.33350328230832815 1107 74-75 0.35855195683663865 1107 76-77 0.2579199118324311 1107 78-79 0.3637227518869608 1107 80-81 0.3203459193925866 1107 82-83 0.3177210901995977 1107 84-85 0.4247865744668715 1107 86-87 0.33970400461531014 1107 88-89 0.3634362049172566 1107 90-91 0.4203201124007343 1107 92-93 0.37120738397079833 1107 94-95 0.4180345021767842 1107 96-97 0.49110023947479675 1107 98-99 0.3300030506609666 1107 100-101 0.47636561338856254 1108 1 0.16505403889873804 1108 2 0.10890695870723732 1108 3 0.0479806924685775 1108 4 0.09850885933605724 1108 5 0.10441343272864856 1108 6 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-0.42789714683794244 2316 52-53 -0.38709440415969 2316 54-55 -0.30907770502193443 2316 56-57 -0.43811654943879574 2316 58-59 -0.4981380930365802 2316 60-61 -0.26084913944878707 2316 62-63 -0.27512791934905323 2316 64-65 -0.14157339154672854 2316 66-67 -0.18135332532535386 2316 68-69 -0.16474096427253926 2316 70-71 -0.11866545087669778 2316 72-73 -0.07402827290392366 2316 74-75 -0.09565191414102259 2316 76-77 -0.05903808052142878 2316 78-79 -0.047733389778599644 2316 80-81 -0.07441431735716719 2316 82-83 -0.06766048976749417 2316 84-85 0.04794493305141856 2316 86-87 -0.13946062049781816 2316 88-89 -0.04140690449528606 2316 90-91 0.03722539264278524 2316 92-93 0.18402654684097897 2316 94-95 0.20126154662853324 2316 96-97 0.2513747779876212 2316 98-99 0.1875445654520611 2316 100-101 0.2965350094922208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 13.0 5 42.0 6 87.0 7 196.0 8 499.0 9 963.0 10 1726.0 11 2765.0 12 4425.0 13 5963.0 14 7696.0 15 10637.0 16 14851.0 17 20791.0 18 19005.0 19 11776.0 20 10101.0 21 10510.0 22 11213.0 23 12239.0 24 14255.0 25 16955.0 26 20653.0 27 25983.0 28 32834.0 29 41450.0 30 51929.0 31 66026.0 32 85051.0 33 112067.0 34 155474.0 35 225403.0 36 353470.0 37 517940.0 38 335783.0 39 20154.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.17085208074292 19.539938456106633 15.342605298106893 22.946604165043556 2 15.870838183017513 20.858062032432287 38.583209324000926 24.687890460549273 3 18.999250315745396 24.05383318287118 30.458027812610457 26.48888868877297 4 13.662450122335917 17.023288400285647 35.77202204117727 33.542239436201164 5 14.101975091773078 37.09069207035561 33.97117311893341 14.83615971893791 6 32.8579629750533 35.908795169601994 16.573019921789697 14.660221933555013 7 28.664098679159906 32.7776364459113 20.50096018118495 18.057304693743852 8 26.678583569532655 34.59777075871198 19.66595150240777 19.057694169347595 9 26.22152381767139 16.656002989731803 20.405639966950854 36.716833225645956 10-11 25.054402762800354 26.555459750060223 26.85434287811215 21.535794609027278 12-13 25.750428270996423 23.97446291361162 27.69450505065969 22.580603764732263 14-15 22.99317629737025 25.267150997215136 25.875250738990562 25.86442196642405 16-17 22.331045257965677 28.6110804683613 26.621355420127106 22.436518853545916 18-19 22.312111790754795 27.0130373018571 29.02772931220409 21.64712159518401 20-21 22.668740868147875 26.013863530450017 29.453383372318413 21.864012229083695 22-23 22.92010508241279 25.728441344332666 29.201726218845447 22.1497273544091 24-25 22.771126162856927 26.069855389474057 29.13737527190174 22.02164317576728 26-27 22.40584786395328 26.252868470564028 29.179952303410282 22.16133136207241 28-29 22.421581905844253 26.377449664913037 29.26931950600563 21.93164892323708 30-31 22.49770929811093 26.15882499938089 29.06690200298523 22.27656369952295 32-33 22.434672784210257 26.231429555570063 29.107943276142706 22.225954384076978 34-35 22.987210341590366 26.025277642074172 28.903277223331614 22.084234793003848 36-37 22.86311846136875 25.865817774946137 28.81725489489787 22.453808868787245 38-39 22.513535965708137 26.228435321159782 28.976309527293687 22.281719185838398 40-41 22.88945382044722 26.200201898494225 28.77026526868619 22.14007901237236 42-43 22.633347825724755 26.21074699008941 28.453412665479505 22.70249251870633 44-45 22.78909558361681 26.306488033193226 28.408778284821484 22.49563809836848 46-47 22.95715543228415 26.678516030410616 27.824902574816463 22.53942596248877 48-49 23.04027357847033 26.802990632323777 27.544277522755056 22.61245826645084 50-51 23.013010286208285 26.949775657549637 27.456611742351754 22.58060231389032 52-53 23.192889481232005 26.98800280062226 27.147980467685905 22.67112725045983 54-55 23.09185791537779 26.919826379039414 27.298879298311967 22.689436407270826 56-57 23.065015410176347 27.295215753725344 27.250684959262657 22.38908387683566 58-59 23.130100610778793 26.77271059261077 27.40246787951921 22.694720917091225 60-61 23.038292430890593 26.772868183895522 27.691580347916528 22.497259037297354 62-63 22.7077109415629 26.983522705527175 27.898700322161613 22.41006603074831 64-65 23.026518110615573 26.955268839475266 27.505937814479037 22.512275235430124 66-67 22.832613291248958 27.034559768746046 27.36628942314836 22.766537516856637 68-69 22.732430260228238 26.9083741757413 27.59252296892975 22.76667259510071 70-71 22.888648249498523 26.903849054564855 27.479620069925502 22.727882626011116 72-73 23.001798791950236 26.703055244750523 27.43932172711043 22.855824236188813 74-75 22.893263422837677 27.324617784851874 26.86627478967192 22.915844002638526 76-77 23.09108551128241 26.923030165223206 27.220067223939466 22.765817099554916 78-79 22.917802637177584 26.92978407742685 27.18449661966694 22.967916665728623 80-81 22.83078973495397 27.184181437097436 27.017674988236934 22.967353839711652 82-83 22.95971020887834 27.12520993646748 27.05429306005196 22.860786794602213 84-85 22.847629489381724 27.118736027844133 26.991222165439332 23.042412317334815 86-87 22.808771981170093 26.979763027733817 27.19359188810118 23.01787310299491 88-89 22.96494494435902 26.93338616393546 26.961324847417867 23.14034404428765 90-91 22.82061194773544 26.864606006267948 27.06013315389747 23.25464889209914 92-93 22.662396328698055 26.86670255299481 27.15507644080568 23.315824677501457 94-95 22.65633618273385 26.783877060787457 27.159349553228708 23.400437203249982 96-97 22.65716916523896 26.65170299896215 27.147440154709617 23.54368768108927 98-99 22.40276932276226 26.764641785507166 26.917687572267866 23.914901319462707 100-101 22.35132893750525 26.71277309830672 27.28739217870453 23.6485057854835 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 373.0 1 284.5 2 244.0 3 314.5 4 297.0 5 323.0 6 594.5 7 1260.5 8 2094.0 9 2632.0 10 2673.0 11 2631.5 12 2810.5 13 3023.5 14 3240.5 15 3369.5 16 3475.0 17 3657.5 18 3837.5 19 3904.0 20 4077.0 21 4548.0 22 5235.0 23 6050.0 24 7069.0 25 8654.0 26 10828.5 27 12834.5 28 14138.5 29 16172.0 30 19291.5 31 22572.0 32 26563.5 33 29848.0 34 33738.5 35 38315.0 36 44192.5 37 51034.0 38 55156.0 39 57221.5 40 59067.0 41 64275.5 42 68969.0 43 72751.0 44 78743.5 45 82797.5 46 85210.5 47 90394.0 48 96949.0 49 98326.5 50 96055.0 51 91530.0 52 86617.5 53 86201.5 54 85303.0 55 78282.5 56 69457.0 57 60687.0 58 53170.0 59 47375.0 60 39544.5 61 31246.0 62 24366.5 63 18472.5 64 13572.0 65 9965.0 66 7112.5 67 5262.0 68 3716.5 69 2381.5 70 1651.0 71 1060.0 72 667.5 73 449.5 74 325.0 75 224.0 76 147.5 77 82.5 78 40.5 79 27.0 80 23.0 81 15.0 82 10.0 83 6.5 84 2.5 85 2.5 86 2.0 87 1.0 88 1.5 89 1.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01828058903119632 2 0.0 3 0.0 4 4.5026081357626405E-5 5 0.03534547386573673 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003894756037434684 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.177999356127036E-4 24-25 1.8010432543050562E-4 26-27 9.680607491889677E-4 28-29 8.554955457949017E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 7.654433830796489E-4 42-43 0.01033348567157526 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.2513040678813205E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0011256520339406602 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012224581088595568 92-93 0.0015083737254804846 94-95 0.0 96-97 0.0 98-99 0.0013958085220864185 100-101 0.0189334672108819 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2220935.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.552201744810205 #Duplication Level Percentage of deduplicated Percentage of total 1 63.36251082147704 23.160392786043484 2 15.751989370658666 11.515397867168431 3 7.0471612721378545 7.7276778164218864 4 3.8906483944342733 5.688470601259339 5 2.371486549649533 4.334152739894679 6 1.5827020831972953 3.471074750817535 7 1.0902204874094479 2.789497144148081 8 0.7979998422908545 2.3334920982193634 9 0.6027120573325722 1.9827407442284812 >10 3.1436758172658625 20.71473765048255 >50 0.2297225672294457 5.797698486306965 >100 0.12361541538380169 8.083785187298407 >500 0.00481461199559727 1.1481871544487592 >1k 3.7035476889209733E-4 0.2273812171848022 >5k 3.7035476889209733E-4 1.025313756077213 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9334 0.42027344339208483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8193 0.36889868456303315 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5195 0.23391049265286917 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.1256520339406601E-4 0.0 18-19 0.0 0.0 0.0 1.8010432543050562E-4 0.0 20-21 0.0 0.0 0.0 1.8010432543050562E-4 0.0 22-23 0.0 0.0 0.0 3.37695610182198E-4 0.0 24-25 0.0 0.0 0.0 4.727738542550773E-4 0.0 26-27 0.0 0.0 0.0 8.780085864737149E-4 0.0 28-29 0.0 0.0 0.0 0.0021837649458448806 0.0 30-31 0.0 0.0 0.0 0.004750251583229586 0.0 32-33 0.0 0.0 0.0 0.009252859718992226 0.0 34-35 0.0 0.0 0.0 0.014160702586973505 0.0 36-37 0.0 0.0 0.0 0.01996906708210731 0.0 38-39 0.0 0.0 0.0 0.025057014275519096 0.0 40-41 0.0 0.0 0.0 0.0325763698622427 0.0 42-43 0.0 0.0 0.0 0.04173917741851968 0.0 44-45 0.0 0.0 0.0 0.04966376773746192 0.0 46-47 0.0 0.0 0.0 0.05977212300224905 0.0 48-49 4.5026081357626405E-5 0.0 0.0 0.07035325212129126 0.0 50-51 4.5026081357626405E-5 0.0 0.0 0.08539196329473847 0.0 52-53 4.5026081357626405E-5 0.0 0.0 0.12161544574694891 0.0 54-55 4.5026081357626405E-5 0.0 0.0 0.1715268569318778 0.0 56-57 4.5026081357626405E-5 0.0 0.0 0.21581000794710337 0.0 58-59 4.5026081357626405E-5 0.0 0.0 0.2778559480579125 0.0 60-61 4.5026081357626405E-5 0.0 0.0 0.3478489915283428 0.0 62-63 4.5026081357626405E-5 0.0 0.0 0.39690490716747673 0.0 64-65 4.5026081357626405E-5 0.0 0.0 0.44600584888796835 0.0 66-67 4.5026081357626405E-5 0.0 0.0 0.563073660417797 0.0 68-69 4.5026081357626405E-5 0.0 0.0 0.6836760193341993 0.0 70-71 4.5026081357626405E-5 0.0 0.0 0.7499544110926254 0.0 72-73 4.5026081357626405E-5 0.0 0.0 0.8753295346329362 0.0 74-75 4.5026081357626405E-5 0.0 0.0 1.053700355931173 0.0 76-77 4.5026081357626405E-5 0.0 0.0 1.2359209071854873 0.0 78-79 4.5026081357626405E-5 0.0 0.0 1.4247377793586935 0.0 80-81 4.5026081357626405E-5 0.0 0.0 1.6651770538084185 0.0 82-83 4.5026081357626405E-5 0.0 0.0 1.9157471965636095 0.0 84-85 4.5026081357626405E-5 0.0 0.0 2.1948863879402145 0.0 86-87 4.5026081357626405E-5 0.0 0.0 2.5578416297640407 0.0 88-89 4.5026081357626405E-5 0.0 0.0 2.973837595427151 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6625 0.0 37.944767 1 GTATCAA 10190 0.0 32.970634 1 GTATACG 60 0.003948136 31.672567 4 TATCAAC 12690 0.0 26.393805 2 ATCAACG 12600 0.0 26.318394 3 TCAACGC 12650 0.0 26.214369 4 CAACGCA 12895 0.0 25.716307 5 AACGCAG 13170 0.0 25.209728 6 TATACGT 125 2.2539785E-4 22.804247 5 GAGTACT 8970 0.0 22.797476 12-13 GGACCGA 625 0.0 22.039145 6 GTACATG 6780 0.0 22.008076 1 ACGCAGA 15220 0.0 21.753252 7 CGCAGAG 15180 0.0 21.685404 8 GTACTTT 9450 0.0 21.411867 14-15 TACATGG 6970 0.0 20.993868 2 CTATACG 115 0.003720337 20.656023 4 AGAGTAC 12815 0.0 20.108849 10-11 AGTACTT 9380 0.0 20.00324 12-13 GCAGAGT 16185 0.0 19.957321 9 >>END_MODULE