FastQCFastQC Report
Thu 26 May 2016
SRR937766_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937766_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202148
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76260.3462982506171247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61350.27859162962707323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36880.16747284923629113No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28590.12982778632498815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64100.037.7482641
GTATCAA93950.031.3206771
ATCAACG113550.025.6567613
TCAACGC115000.025.3780574
CAACGCA117000.024.9419695
TATCAAC117650.024.8445682
AACGCAG119200.024.5496376
GTACATG67100.024.3001461
TACATGG69900.022.7780152
ACATGGG69200.022.526633
GAGTACT68500.022.43261312-13
CGCAGAG133600.021.3716628
ACGCAGA135600.021.301217
GTACTTT76250.020.27624514-15
AGTACTT73100.019.85134712-13
GCAGAGT140950.019.7848829
AGAGTAC110050.019.70388410-11
GTATAGG5100.019.5743061
CATGGGG44700.018.7151344
GTCTTAG6150.018.5512581
Produced by FastQC (version 0.11.5)