##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937766_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2202148 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.70802053267991 33.0 31.0 34.0 30.0 34.0 2 31.838001805509894 33.0 31.0 34.0 30.0 34.0 3 31.8886614341997 33.0 31.0 34.0 30.0 34.0 4 35.484605939292 37.0 35.0 37.0 33.0 37.0 5 35.45401171946663 37.0 35.0 37.0 33.0 37.0 6 35.53908729113574 37.0 35.0 37.0 33.0 37.0 7 35.47495218305037 37.0 35.0 37.0 33.0 37.0 8 35.459607619469715 37.0 35.0 37.0 33.0 37.0 9 37.07975758214253 39.0 37.0 39.0 33.0 39.0 10-11 36.9694066883788 39.0 37.0 39.0 33.0 39.0 12-13 36.84599286696444 39.0 37.0 39.0 33.0 39.0 14-15 38.00829939677079 40.0 38.0 41.0 33.0 41.0 16-17 37.93947386824137 40.0 38.0 41.0 33.0 41.0 18-19 37.87078343508247 40.0 38.0 41.0 33.0 41.0 20-21 37.68771989893504 40.0 37.0 41.0 32.0 41.0 22-23 37.55304094002764 40.0 37.0 41.0 32.0 41.0 24-25 37.405293377193544 39.5 37.0 41.0 32.0 41.0 26-27 37.5074191198775 40.0 37.0 41.0 32.0 41.0 28-29 37.45066498709441 40.0 37.0 41.0 32.0 41.0 30-31 37.26450265831361 39.5 37.0 41.0 31.5 41.0 32-33 37.04445341548343 39.0 36.0 41.0 31.0 41.0 34-35 36.790925723430036 39.0 36.0 41.0 30.5 41.0 36-37 36.8516166942458 39.0 36.0 41.0 30.0 41.0 38-39 36.825240628695255 39.0 36.0 41.0 30.0 41.0 40-41 36.650475127012356 39.0 36.0 41.0 30.0 41.0 42-43 36.43124008922197 39.0 35.5 41.0 30.0 41.0 44-45 36.2154623576617 39.0 35.0 41.0 30.0 41.0 46-47 35.88460244270594 39.0 35.0 40.5 28.5 41.0 48-49 35.57647919213423 39.0 35.0 40.0 27.5 41.0 50-51 34.66126958769347 38.0 34.0 39.5 26.0 40.5 52-53 34.76185796776602 38.0 34.0 39.5 26.0 40.5 54-55 35.0675724338237 38.0 34.5 40.0 26.0 41.0 56-57 34.78563407182442 38.0 34.0 40.0 25.5 41.0 58-59 34.552840681007815 38.0 34.0 40.0 25.5 41.0 60-61 34.23439069490334 38.0 34.0 40.0 24.5 41.0 62-63 33.85800545649066 37.0 33.0 40.0 23.5 41.0 64-65 33.46807094709347 36.5 33.0 39.0 22.5 41.0 66-67 33.03486141712546 36.0 32.0 39.0 22.0 40.5 68-69 32.54114641704372 35.5 32.0 39.0 20.5 40.0 70-71 32.08310862848455 35.0 31.0 38.0 19.5 40.0 72-73 31.604538387065716 35.0 31.0 37.0 18.0 39.5 74-75 31.107399457257188 35.0 31.0 37.0 14.5 39.0 76-77 30.653401133802085 34.5 30.0 36.0 10.0 39.0 78-79 30.13485446936355 34.0 30.0 36.0 7.0 37.5 80-81 29.635045873392706 34.0 29.0 35.0 4.5 37.0 82-83 29.141217120738478 34.0 29.0 35.0 2.0 36.5 84-85 28.69097149692028 34.0 29.0 35.0 2.0 36.0 86-87 28.2059209916863 33.5 27.5 35.0 2.0 36.0 88-89 27.739757046302067 33.0 26.5 35.0 2.0 35.0 90-91 27.246946617575205 33.0 26.0 35.0 2.0 35.0 92-93 26.69208586343879 32.5 25.0 35.0 2.0 35.0 94-95 26.057604211887664 32.0 24.5 34.0 2.0 35.0 96-97 25.27894015297791 32.0 21.5 34.0 2.0 35.0 98-99 24.337654190363224 31.5 10.0 34.0 2.0 35.0 100-101 21.766410114124938 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18925460521434445 1101 2 0.3562971723206445 1101 3 0.345844871527774 1101 4 0.1546153847083147 1101 5 0.1762037937662555 1101 6 0.1923682508717377 1101 7 0.13629248754543966 1101 8 0.26501998339431054 1101 9 0.26933698399033545 1101 10-11 0.11840890993863695 1101 12-13 0.216511996566787 1101 14-15 0.3775379911707262 1101 16-17 0.46997839975688294 1101 18-19 0.5073066340283816 1101 20-21 0.6744906017232566 1101 22-23 0.5769106938636241 1101 24-25 0.46811548916779344 1101 26-27 0.6434389787250296 1101 28-29 0.5742767495974661 1101 30-31 0.47691344865167906 1101 32-33 0.4923128571061852 1101 34-35 0.5822236660242694 1101 36-37 0.5950450085454406 1101 38-39 0.6178566119408018 1101 40-41 0.7825395355388594 1101 42-43 0.7444137993795579 1101 44-45 0.7005318829631548 1101 46-47 0.7603370624192038 1101 48-49 0.8890980803261641 1101 50-51 0.8888814762555199 1101 52-53 0.74308198210651 1101 54-55 0.8405750167088257 1101 56-57 1.006667710581496 1101 58-59 0.9366776259349976 1101 60-61 1.0382578134856715 1101 62-63 0.8803778048088304 1101 64-65 1.0272359064553171 1101 66-67 1.0425668747198742 1101 68-69 1.155928923595873 1101 70-71 1.149736333844423 1101 72-73 1.1682535290582514 1101 74-75 1.0398546244571314 1101 76-77 1.0575328553895211 1101 78-79 1.3089655970888288 1101 80-81 1.2148277475559865 1101 82-83 1.4070163828658373 1101 84-85 1.4772378852053443 1101 86-87 1.774555120734668 1101 88-89 1.8572182589968627 1101 90-91 2.0154822248153472 1101 92-93 2.2329232288696055 1101 94-95 2.0621368304858443 1101 96-97 2.232087184070515 1101 98-99 2.3822052609188056 1101 100-101 2.6921243572070956 1102 1 0.13389964304776214 1102 2 0.20481787114327688 1102 3 0.20173213086926722 1102 4 0.2134299863618807 1102 5 0.2037970626318426 1102 6 0.22005135584120694 1102 7 0.2551301690435892 1102 8 0.07684226859107923 1102 9 0.0906611535541586 1102 10-11 0.15714485022319735 1102 12-13 0.2655278895230566 1102 14-15 0.4423150807731062 1102 16-17 0.48422728228339196 1102 18-19 0.39649233913224435 1102 20-21 0.41704866652211336 1102 22-23 0.519792168636485 1102 24-25 0.5097361178744464 1102 26-27 0.5814776301609541 1102 28-29 0.48077766986975234 1102 30-31 0.4454332710245126 1102 32-33 0.4290012645969057 1102 34-35 0.4990149102908319 1102 36-37 0.5814108094744768 1102 38-39 0.6406475932724263 1102 40-41 0.7292243641929232 1102 42-43 0.8078086546192651 1102 44-45 0.8320003015555812 1102 46-47 0.7316594060459565 1102 48-49 0.7381523909984011 1102 50-51 0.6616331634213779 1102 52-53 0.6555174169716835 1102 54-55 0.7084131802543538 1102 56-57 0.7810619376988512 1102 58-59 0.8298745495962194 1102 60-61 0.7094926582079779 1102 62-63 0.7765253697391543 1102 64-65 0.7555027269876149 1102 66-67 0.7484114801713915 1102 68-69 0.7048521319864278 1102 70-71 0.7887376923415985 1102 72-73 0.7224554484832986 1102 74-75 0.565023633192407 1102 76-77 0.7624060623193181 1102 78-79 0.7467310883389366 1102 80-81 0.7709745821114815 1102 82-83 0.8143044280906473 1102 84-85 0.9355017112008461 1102 86-87 0.8831800440453108 1102 88-89 1.009481836664925 1102 90-91 1.2451834284730623 1102 92-93 1.3264472874164532 1102 94-95 1.1633458929767144 1102 96-97 1.2957541039337883 1102 98-99 1.6445192344500192 1102 100-101 1.769939075720636 1103 1 0.17383883116970011 1103 2 0.23757967812480274 1103 3 0.13879815519401717 1103 4 0.14373562528148653 1103 5 0.18131878115971745 1103 6 0.2563750013948365 1103 7 0.26478030330357427 1103 8 0.24301901791977798 1103 9 0.18582858365998334 1103 10-11 0.25897591956452715 1103 12-13 0.276326341584344 1103 14-15 0.4054733496089966 1103 16-17 0.4056180578322781 1103 18-19 0.29695632586419407 1103 20-21 0.24508847066046968 1103 22-23 0.14075094292174128 1103 24-25 0.2602230077812493 1103 26-27 0.01116093453624245 1103 28-29 0.2091491751611727 1103 30-31 0.187553789110126 1103 32-33 0.23742134041431484 1103 34-35 0.15160494346094566 1103 36-37 0.3079018851339299 1103 38-39 0.46715406934255554 1103 40-41 0.42269695935956264 1103 42-43 0.4084629746319024 1103 44-45 0.373799780310911 1103 46-47 0.39570813452486675 1103 48-49 0.4464891466452201 1103 50-51 0.2861818129238287 1103 52-53 0.37572788925094613 1103 54-55 0.3080392266925145 1103 56-57 0.5908771327707143 1103 58-59 0.4598283483641836 1103 60-61 0.48210322525899585 1103 62-63 0.4086743193521656 1103 64-65 0.5631547566827635 1103 66-67 0.694589809029388 1103 68-69 0.7471823325866467 1103 70-71 0.780299451937239 1103 72-73 0.7998962161345382 1103 74-75 0.8688634689213615 1103 76-77 0.7513817375680958 1103 78-79 0.714567922962221 1103 80-81 0.9139614720182898 1103 82-83 0.5734202865648221 1103 84-85 0.8634455589342203 1103 86-87 0.8889488799322152 1103 88-89 0.9366070933384307 1103 90-91 0.9147999977354253 1103 92-93 1.0651512836255783 1103 94-95 1.1111558756018916 1103 96-97 1.2042448636873821 1103 98-99 1.4391243521249528 1103 100-101 1.7282268659591438 1104 1 0.16705733179749416 1104 2 0.2942397027806507 1104 3 0.2876991841674936 1104 4 0.2187025260331268 1104 5 0.30342490010426815 1104 6 0.3265976448898087 1104 7 0.36299930730587704 1104 8 0.38426381559171574 1104 9 0.46698756072700576 1104 10-11 0.44182623498090123 1104 12-13 0.43786795925905864 1104 14-15 0.5441769607987226 1104 16-17 0.560504589619903 1104 18-19 0.5798028422276644 1104 20-21 0.6726069103134691 1104 22-23 0.6217647011918075 1104 24-25 0.6696982256094444 1104 26-27 0.6350871797233211 1104 28-29 0.555257140445427 1104 30-31 0.549396481726852 1104 32-33 0.4829237366669261 1104 34-35 0.7064220475568916 1104 36-37 0.665666484419333 1104 38-39 0.6691886521210719 1104 40-41 0.7594652380276941 1104 42-43 0.8956744128203624 1104 44-45 0.7922150243905648 1104 46-47 0.8748051756325168 1104 48-49 0.8249516509428929 1104 50-51 0.6787115452615495 1104 52-53 0.6860701459263581 1104 54-55 0.7168275757570939 1104 56-57 0.9039944076340944 1104 58-59 1.0482949936295185 1104 60-61 1.0437268074999508 1104 62-63 0.9610663930082026 1104 64-65 0.9064729824490811 1104 66-67 0.925598913459659 1104 68-69 0.8454610766957416 1104 70-71 1.0285251042821102 1104 72-73 1.054710660893459 1104 74-75 0.9892697016475402 1104 76-77 0.9691779259783253 1104 78-79 0.8579048590640532 1104 80-81 1.0143247564514795 1104 82-83 1.1224726128198874 1104 84-85 1.0787976266637394 1104 86-87 0.8513019753055389 1104 88-89 1.0415169893013712 1104 90-91 1.1542439493582854 1104 92-93 1.1740139991505991 1104 94-95 1.0636522332599263 1104 96-97 1.229199987375047 1104 98-99 1.43102638594792 1104 100-101 1.2538293482603073 1105 1 0.19274579689391302 1105 2 0.20438457026693868 1105 3 0.2047554999052963 1105 4 0.2091505316393878 1105 5 0.36945431677010987 1105 6 0.32025096635727834 1105 7 0.3634213082840958 1105 8 0.25893087618601385 1105 9 0.26873807663108096 1105 10-11 0.30005867690284305 1105 12-13 0.3373925438560619 1105 14-15 0.23631215964360308 1105 16-17 0.46628667858329464 1105 18-19 0.38766809375736244 1105 20-21 0.5414303607772197 1105 22-23 0.5406587703891574 1105 24-25 0.5549186884684119 1105 26-27 0.4754649719330999 1105 28-29 0.32103083442671476 1105 30-31 0.3387682272075736 1105 32-33 0.38574663947519383 1105 34-35 0.5076395451496367 1105 36-37 0.5452910431648377 1105 38-39 0.5125195504291895 1105 40-41 0.6399108296262241 1105 42-43 0.5226309525744739 1105 44-45 0.6372120834445809 1105 46-47 0.7589451898823683 1105 48-49 0.8300316509789241 1105 50-51 0.7019423162938239 1105 52-53 0.6833582154137474 1105 54-55 0.6934204830781141 1105 56-57 0.7828925121876509 1105 58-59 0.8635040530041991 1105 60-61 0.7409507886850975 1105 62-63 0.9999382227089697 1105 64-65 0.9497724446117601 1105 66-67 0.9878321228198743 1105 68-69 1.0521476529211853 1105 70-71 0.9873428530037174 1105 72-73 1.0279739489701463 1105 74-75 1.0093955903491754 1105 76-77 0.9940370262920553 1105 78-79 1.0755285566933672 1105 80-81 1.069367960884609 1105 82-83 1.187296151557053 1105 84-85 1.4238293645601452 1105 86-87 1.2466488853306075 1105 88-89 1.1279920606181832 1105 90-91 1.2151177030592386 1105 92-93 1.522352837562508 1105 94-95 1.5962037313408821 1105 96-97 1.5892643278870509 1105 98-99 1.8765688936515765 1105 100-101 1.8561026589728264 1106 1 0.1241347672050388 1106 2 0.25854949879706624 1106 3 0.20335748156647782 1106 4 0.20337240920849808 1106 5 0.17039770749595817 1106 6 0.2350094815478414 1106 7 0.22496581244210745 1106 8 0.2805822711035333 1106 9 0.33684661479949796 1106 10-11 0.14124591383024665 1106 12-13 -0.02014209917089005 1106 14-15 0.09778616657353467 1106 16-17 0.17341429287758814 1106 18-19 0.18296746580197976 1106 20-21 0.24585483041808942 1106 22-23 0.1549117197440566 1106 24-25 0.14672608898117545 1106 26-27 0.1519887059115419 1106 28-29 0.4415967310480937 1106 30-31 0.4380263366415349 1106 32-33 0.5851078974849742 1106 34-35 0.2404965274760329 1106 36-37 0.4703796550002082 1106 38-39 0.3997756657986926 1106 40-41 0.2538600578405905 1106 42-43 0.45398626443459733 1106 44-45 0.2954171392089435 1106 46-47 0.4767838773829993 1106 48-49 0.6987729680261623 1106 50-51 0.5355367897351826 1106 52-53 0.4738992895900864 1106 54-55 0.4645397034317753 1106 56-57 0.45704451799281287 1106 58-59 0.4396527205270857 1106 60-61 0.19672822204534413 1106 62-63 0.4705115930966386 1106 64-65 0.6882859018965348 1106 66-67 0.9168677843462589 1106 68-69 1.0392609716497603 1106 70-71 0.7434779670369593 1106 72-73 0.6902144409877984 1106 74-75 0.7495473131053139 1106 76-77 0.8338082230328574 1106 78-79 0.7359704417998465 1106 80-81 0.6084122941000913 1106 82-83 0.5923785246527693 1106 84-85 0.6791697946144417 1106 86-87 0.5005682396820106 1106 88-89 0.6488449286627471 1106 90-91 0.9209871008485919 1106 92-93 0.8758834500562038 1106 94-95 1.1190276064728018 1106 96-97 1.001788398980061 1106 98-99 1.258320792173734 1106 100-101 1.352775640353606 1107 1 0.41917142174224864 1107 2 0.49032260300447916 1107 3 0.545244627836027 1107 4 0.4172676471238361 1107 5 0.47382618177768165 1107 6 0.468344245916434 1107 7 0.3920981379831616 1107 8 0.42455277574953243 1107 9 0.531417038048211 1107 10-11 0.5683599679130751 1107 12-13 0.5214160234711258 1107 14-15 0.7633063450293562 1107 16-17 0.6701954938825168 1107 18-19 0.6585676849220974 1107 20-21 0.8526516755005176 1107 22-23 0.8912772450742423 1107 24-25 0.8664596073187525 1107 26-27 0.8015637743007247 1107 28-29 0.8254804119226478 1107 30-31 0.830222157217186 1107 32-33 0.7732338537742862 1107 34-35 0.8535702366679203 1107 36-37 1.091958006756684 1107 38-39 0.900350941974196 1107 40-41 0.8877841797716641 1107 42-43 0.8899410254030542 1107 44-45 0.7780225049042855 1107 46-47 0.9176379306758946 1107 48-49 0.8175214761719047 1107 50-51 0.722029305134555 1107 52-53 0.8273391835331978 1107 54-55 0.7676360630497925 1107 56-57 0.7516613067840225 1107 58-59 0.8247089344542573 1107 60-61 1.1285590255945408 1107 62-63 1.1179437148010365 1107 64-65 1.1274258039106897 1107 66-67 1.1942996141194584 1107 68-69 1.32606482308082 1107 70-71 1.1830964644842865 1107 72-73 1.1583575353554103 1107 74-75 1.134329626883261 1107 76-77 1.1651614614942751 1107 78-79 1.2877708845049192 1107 80-81 1.2990624248680973 1107 82-83 1.4082106401750956 1107 84-85 1.4784858170004078 1107 86-87 1.2386635431960045 1107 88-89 1.485223161586056 1107 90-91 1.486476197218515 1107 92-93 1.3823038756231973 1107 94-95 1.8359338570631003 1107 96-97 1.7064323373722772 1107 98-99 1.9197182152799144 1107 100-101 2.2910067777347116 1108 1 0.28499873968066325 1108 2 0.24810968608989725 1108 3 0.2703319118711818 1108 4 0.30246958851536476 1108 5 0.46223396380895565 1108 6 0.29500208127236505 1108 7 0.259250526740459 1108 8 0.1910027759366386 1108 9 0.4059768757915876 1108 10-11 0.27550715517802615 1108 12-13 0.3501784050810457 1108 14-15 0.37393703533100364 1108 16-17 0.32164629861699723 1108 18-19 0.36694470704902216 1108 20-21 0.29026520087743535 1108 22-23 0.40345491158748104 1108 24-25 0.46188435220239654 1108 26-27 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-0.9246227668864222 2316 94-95 -0.8665622236845572 2316 96-97 -0.9799378081746752 2316 98-99 -1.1234109629822804 2316 100-101 -0.8096149933986503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7690.0 3 1347.0 4 1394.0 5 1541.0 6 1881.0 7 2123.0 8 2747.0 9 3401.0 10 4421.0 11 5684.0 12 7256.0 13 9198.0 14 10511.0 15 12751.0 16 16063.0 17 20555.0 18 18193.0 19 14032.0 20 14281.0 21 15288.0 22 16792.0 23 18718.0 24 21314.0 25 24252.0 26 28277.0 27 33899.0 28 40259.0 29 48741.0 30 59316.0 31 74686.0 32 94667.0 33 122779.0 34 165035.0 35 229325.0 36 328327.0 37 422649.0 38 276856.0 39 25895.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85752672378732 18.719107274526657 14.864578679356907 23.558787322329113 2 15.37487998404795 20.074861728420913 39.666882608123615 24.883375679407514 3 18.72455241383329 23.614631588147887 30.41887910439064 27.24193689362818 4 13.134433271858056 16.351163894967875 36.39516932713027 34.119233506043805 5 14.16455421888601 36.53177959156915 34.34853749412703 14.95512869541781 6 32.68639439872672 35.32632385500681 17.141932836045 14.845348910221468 7 28.53121623312367 32.47211637984964 20.86352210557445 18.133145281452244 8 26.465938275564778 34.09589193787975 20.178799314830105 19.25937047172537 9 26.14232172246113 16.579630247267758 20.635343869312962 36.642704160958154 10-11 24.796965580441718 26.161244035575987 27.40203078783748 21.63975959614482 12-13 25.56047456007644 23.652633853923767 27.931978205726114 22.854913380273683 14-15 22.936198713518248 24.998714610263008 25.963712423962942 26.101374252255805 16-17 22.316000949919065 28.21832852459553 26.80122943548057 22.664441090004832 18-19 22.32881501905954 26.8536276823202 28.929055558045214 21.88850174057504 20-21 22.75930620428783 26.003964611914988 29.117424617214787 22.119304566582393 22-23 22.953337961745536 25.63959894777932 28.961023067145035 22.44604002333011 24-25 22.755666140282155 25.968931939892986 28.814047050677132 22.461354869147726 26-27 22.361269964322858 26.095018029649967 28.929854061288978 22.613857944738193 28-29 22.38392402353494 26.268035617721686 29.00763636636632 22.340403992377055 30-31 22.396390207060342 26.061553930779695 28.851909313848157 22.690146548311805 32-33 22.355592754311292 26.128882768636956 28.83988491236819 22.675639564683557 34-35 22.916668562417364 25.9562773158718 28.612110298112103 22.514943823598728 36-37 22.706106177419112 25.751483860753876 28.663044531517624 22.879365430309385 38-39 22.440215535897746 26.120274234881 28.71337369267507 22.72613653654619 40-41 22.782485364934477 26.072284794201607 28.60910529764468 22.536124543219245 42-43 22.535125406937915 26.054285381534438 28.3448295000646 23.065759711463045 44-45 22.64776865632599 26.163803986597607 28.35117816856716 22.837249188509247 46-47 22.760618485177623 26.49664200535687 27.844502954239147 22.898236555226358 48-49 22.78688871432169 26.557517426955346 27.649510320811586 23.006083537911376 50-51 22.784706750011487 26.689049777131917 27.62472263763702 22.90152083521957 52-53 22.916115880170093 26.69446195079666 27.336703653733707 23.052718515299542 54-55 22.851231294471372 26.48486986681989 27.54254955442474 23.121349284284 56-57 22.900510573667006 27.006228821336514 27.39821675625037 22.695043848746106 58-59 22.912359908339823 26.434635264148476 27.66743050145849 22.98557432605321 60-61 22.817948958782726 26.39008822379593 27.88718415924267 22.904778658178675 62-63 22.553915888371225 26.6421417841454 28.08782106463766 22.71612126284571 64-65 22.763886124962333 26.648168890758882 27.689487539587326 22.898457444691463 66-67 22.583450591232253 26.740941500381055 27.482583023027274 23.193024885359414 68-69 22.536474664979387 26.58544734272664 27.817861049090265 23.060216943203713 70-71 22.622501457890394 26.567107081480835 27.743449500587253 23.06694196004152 72-73 22.699663330140922 26.413516465846648 27.659556563743138 23.227263640269292 74-75 22.60895752973037 26.96377855610091 27.25483844993092 23.1724254642378 76-77 22.791342663237117 26.65729900718427 27.448167222274407 23.103191107304205 78-79 22.742343466076385 26.620166453658207 27.446680218235297 23.19080986203011 80-81 22.643855512882503 26.809489216778438 27.335902207500197 23.210753062838858 82-83 22.79724112210741 26.664695823024303 27.436076444476786 23.1019866103915 84-85 22.63924354462136 26.66220499711538 27.364087926271935 23.334463531991332 86-87 22.643202561539834 26.689680501119422 27.52943530827054 23.137681629070205 88-89 22.68292710660822 26.513864014964156 27.415351902073493 23.387856976354136 90-91 22.60385352090125 26.319709687294317 27.601517791796702 23.47491900000773 92-93 22.600381209002464 26.391980283202678 27.59146275593504 23.41617575185982 94-95 22.542956514382443 26.286296102648514 27.60936106669384 23.561386316275204 96-97 22.505967846408012 26.21806383412298 27.542862055413636 23.73310626405537 98-99 22.366506284211297 26.211477391259645 27.533347556529243 23.88866876799981 100-101 22.65892522710914 25.723343601429573 27.99201294485134 23.625718226609948 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2515.0 1 1470.0 2 437.0 3 428.0 4 359.0 5 361.5 6 558.0 7 1048.5 8 1821.0 9 2347.0 10 2322.0 11 2269.5 12 2459.5 13 2649.0 14 2798.0 15 2921.0 16 3005.5 17 3106.0 18 3209.0 19 3308.5 20 3487.0 21 3941.5 22 4688.0 23 5574.5 24 6696.5 25 8296.5 26 10461.5 27 12512.0 28 13813.0 29 15725.0 30 18815.0 31 22534.0 32 26590.5 33 29930.5 34 33689.0 35 37871.0 36 44071.5 37 50925.5 38 54817.5 39 57129.5 40 58905.5 41 63641.5 42 68394.0 43 72568.0 44 78697.0 45 82490.5 46 85264.5 47 90468.0 48 96083.5 49 97496.0 50 95151.0 51 90438.0 52 85366.0 53 85165.0 54 84438.0 55 77524.5 56 69156.5 57 60501.5 58 52843.5 59 46677.0 60 38984.5 61 31080.0 62 24221.5 63 18225.5 64 13710.5 65 10251.0 66 7322.0 67 5293.0 68 3865.0 69 2672.0 70 1802.0 71 1184.5 72 782.0 73 542.0 74 380.0 75 261.5 76 182.5 77 120.0 78 71.5 79 46.5 80 30.0 81 23.0 82 24.5 83 18.5 84 12.0 85 11.0 86 9.5 87 9.0 88 7.0 89 5.0 90 6.0 91 6.5 92 3.0 93 2.0 94 3.0 95 4.0 96 2.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29266879428630593 2 0.2527078107375163 3 0.24866630217405916 4 0.2543425782463304 5 0.2562952172151917 6 0.1980793298179777 7 0.18949680039670358 8 0.18858859622514018 9 0.20021360962115173 10-11 0.20114451889700422 12-13 0.1963083316834291 14-15 0.19848802169518126 16-17 0.1846379080788394 18-19 0.18924704424952365 20-21 0.17939302898806075 22-23 0.18754416142784228 24-25 0.1877712124707331 26-27 0.1862726755876535 28-29 0.1956952938676238 30-31 0.19244846395428464 32-33 0.19728465116785973 34-35 0.19299338645722267 36-37 0.18150460368694565 38-39 0.17317183041285147 40-41 0.17012934643811406 42-43 0.18273067931855624 44-45 0.1831393711957598 46-47 0.1832528967172052 48-49 0.19669431845634353 50-51 0.19122238832267405 52-53 0.2058444754848448 54-55 0.18320748650862703 56-57 0.1697660647694887 58-59 0.17303559978711694 60-61 0.1630907641084977 62-63 0.16804047684351825 64-65 0.1673593237148457 66-67 0.16558832558029704 68-69 0.1697660647694887 70-71 0.17101484550538837 72-73 0.16817670746925276 74-75 0.18050557909822593 76-77 0.17276313853564793 78-79 0.1761234939704325 80-81 0.17160517821690457 82-83 0.1556661950059669 84-85 0.15528020823305247 86-87 0.15759612887053912 88-89 0.16168304764257443 90-91 0.1676090798620256 92-93 0.16422601932295197 94-95 0.16422601932295197 96-97 0.16801777173922916 98-99 0.17532881532031452 100-101 0.16304535389991953 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2202148.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.741204762114435 #Duplication Level Percentage of deduplicated Percentage of total 1 65.30510279565297 25.299983594173238 2 15.186524880181034 11.766885400160778 3 6.669954877278491 7.752062629641297 4 3.656159720363246 5.665761294783503 5 2.252887206676879 4.36397822899085 6 1.4516781329957102 3.374385587944248 7 0.9972712794770979 2.7044843589118672 8 0.7340038887185565 2.2748955959227084 9 0.5649525596799603 1.9698248515897816 >10 2.856599339967362 19.822121147533363 >50 0.21127890265743274 5.6070818642267914 >100 0.10947678315606375 7.482758496636372 >500 0.003170288464986123 0.7668566499922264 >1k 7.045085477746909E-4 0.5229436497989443 >5k 2.3483618259156365E-4 0.6259766496940912 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7626 0.3462982506171247 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6135 0.27859162962707323 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3688 0.16747284923629113 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2859 0.12982778632498815 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.541020857817004E-5 0.0 18-19 0.0 0.0 0.0 6.811531286725506E-5 0.0 20-21 0.0 0.0 0.0 1.3623062573451012E-4 0.0 22-23 0.0 0.0 0.0 3.178714600471903E-4 0.0 24-25 0.0 0.0 0.0 5.449225029380405E-4 0.0 26-27 0.0 0.0 0.0 9.082041715634008E-4 0.0 28-29 0.0 0.0 0.0 0.002429446158932097 0.0 30-31 0.0 0.0 0.0 0.005857916906583936 0.0 32-33 0.0 0.0 0.0 0.01017188672151009 0.0 34-35 0.0 0.0 0.0 0.015439470916577814 0.0 36-37 0.0 0.0 0.0 0.0206162346944892 0.0 38-39 0.0 0.0 0.0 0.026292510766760452 0.0 40-41 0.0 0.0 0.0 0.03457987383227648 0.0 42-43 0.0 0.0 0.0 0.04450200440660664 0.0 44-45 0.0 0.0 0.0 0.05490094217100758 0.0 46-47 0.0 0.0 0.0 0.06707087806995715 0.0 48-49 0.0 0.0 0.0 0.07865048125739052 0.0 50-51 0.0 0.0 0.0 0.0941126572782574 0.0 52-53 0.0 0.0 0.0 0.1343006918699379 0.0 54-55 0.0 0.0 0.0 0.19222141291139377 0.0 56-57 0.0 0.0 0.0 0.23726833982093848 0.0 58-59 0.0 0.0 0.0 0.3071546508227422 0.0 60-61 0.0 0.0 0.0 0.3878940016747285 0.0 62-63 0.0 0.0 0.0 0.44447512156312835 0.0 64-65 0.0 0.0 0.0 0.49551619600499147 0.0 66-67 0.0 0.0 0.0 0.6186686816689886 0.0 68-69 0.0 0.0 0.0 0.7407313223271097 0.0 70-71 0.0 0.0 0.0 0.8057360359067601 0.0 72-73 0.0 0.0 0.0 0.9381294990164148 0.0 74-75 0.0 0.0 0.0 1.1222678948008944 0.0 76-77 0.0 0.0 0.0 1.3083362244499461 0.0 78-79 0.0 0.0 0.0 1.5000127148584017 0.0 80-81 0.0 0.0 0.0 1.7393699242739362 0.0 82-83 0.0 0.0 0.0 1.996754986495004 0.0 84-85 0.0 0.0 0.0 2.2815678146972864 0.0 86-87 0.0 0.0 0.0 2.6509344512721214 0.0 88-89 0.0 4.541020857817004E-5 0.0 3.0690489467556223 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6410 0.0 37.748264 1 GTATCAA 9395 0.0 31.320677 1 ATCAACG 11355 0.0 25.656761 3 TCAACGC 11500 0.0 25.378057 4 CAACGCA 11700 0.0 24.941969 5 TATCAAC 11765 0.0 24.844568 2 AACGCAG 11920 0.0 24.549637 6 GTACATG 6710 0.0 24.300146 1 TACATGG 6990 0.0 22.778015 2 ACATGGG 6920 0.0 22.52663 3 GAGTACT 6850 0.0 22.432613 12-13 CGCAGAG 13360 0.0 21.371662 8 ACGCAGA 13560 0.0 21.30121 7 GTACTTT 7625 0.0 20.276245 14-15 AGTACTT 7310 0.0 19.851347 12-13 GCAGAGT 14095 0.0 19.784882 9 AGAGTAC 11005 0.0 19.703884 10-11 GTATAGG 510 0.0 19.574306 1 CATGGGG 4470 0.0 18.715134 4 GTCTTAG 615 0.0 18.551258 1 >>END_MODULE