FastQCFastQC Report
Thu 26 May 2016
SRR937766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202148
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94140.4274917035548928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80650.36623333218294135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.2322278066687616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67800.035.8817671
GTATCAA101900.031.6613271
ATCAACG125250.025.6782593
TCAACGC127050.025.3144594
TATCAAC130000.024.886192
CAACGCA129700.024.8338685
AACGCAG133000.024.2490046
GTACATG67350.024.1280421
TACATGG67600.022.98032
GTATACG1050.002195062622.6222274
GAGTACT83600.022.32862912-13
ACATGGG69650.021.9628853
AGTACTT88900.021.5050212-13
ACGCAGA149900.021.3566957
CGCAGAG148950.021.2059088
GTACTTT88050.021.1731814-15
CCCTATA2257.1486284E-821.1140792
AGAGTAC123250.020.81050310-11
GCAGAGT158450.019.5148169
CATGGGG45050.019.297944
Produced by FastQC (version 0.11.5)