##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937766_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2202148 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18795239920296 33.0 31.0 34.0 31.0 34.0 2 32.3928868541079 34.0 31.0 34.0 31.0 34.0 3 32.48881819023971 34.0 31.0 34.0 31.0 34.0 4 36.12709863278944 37.0 35.0 37.0 35.0 37.0 5 36.03157962135152 37.0 35.0 37.0 35.0 37.0 6 36.06310157173814 37.0 35.0 37.0 35.0 37.0 7 36.00345753328114 37.0 35.0 37.0 35.0 37.0 8 35.979781558732654 37.0 35.0 37.0 35.0 37.0 9 37.6554223421859 39.0 37.0 39.0 35.0 39.0 10-11 37.5887694650859 39.0 37.0 39.0 35.0 39.0 12-13 37.49512748461956 39.0 37.0 39.0 35.0 39.0 14-15 38.725142224773265 40.0 38.0 41.0 34.5 41.0 16-17 38.68147690345971 40.0 38.0 41.0 34.5 41.0 18-19 38.6558998305291 40.0 38.0 41.0 34.0 41.0 20-21 38.53677568446807 40.0 38.0 41.0 34.0 41.0 22-23 38.377882867091586 40.0 38.0 41.0 34.0 41.0 24-25 38.44233652779014 40.0 38.0 41.0 34.0 41.0 26-27 38.32336450592785 40.0 38.0 41.0 34.0 41.0 28-29 38.17544279494385 40.0 38.0 41.0 34.0 41.0 30-31 38.239396262194916 40.0 38.0 41.0 34.0 41.0 32-33 38.180828218630175 40.0 38.0 41.0 34.0 41.0 34-35 38.06027660266249 40.0 37.0 41.0 33.0 41.0 36-37 37.911642178454855 40.0 37.0 41.0 33.0 41.0 38-39 37.777262699873035 40.0 37.0 41.0 33.0 41.0 40-41 37.61027119884767 40.0 37.0 41.0 33.0 41.0 42-43 37.39518438361091 40.0 37.0 41.0 32.5 41.0 44-45 37.09282414260986 40.0 36.0 41.0 31.5 41.0 46-47 36.75908249581772 39.5 36.0 41.0 31.0 41.0 48-49 36.44079303480056 39.0 36.0 41.0 30.0 41.0 50-51 36.18236966816036 39.0 35.5 41.0 30.0 41.0 52-53 35.93037252718709 39.0 35.0 40.0 29.0 41.0 54-55 35.69593324336057 39.0 35.0 40.0 28.5 41.0 56-57 35.4111617384481 38.0 35.0 40.0 28.0 41.0 58-59 35.13092671337258 38.0 34.0 40.0 28.0 41.0 60-61 34.75868424828849 38.0 34.0 40.0 26.5 41.0 62-63 34.387800002542974 37.0 34.0 40.0 26.0 41.0 64-65 33.97174463296744 36.5 33.0 39.0 26.0 41.0 66-67 33.509124500260654 36.0 33.0 39.0 25.5 40.0 68-69 33.059327529303204 35.5 32.0 38.5 24.5 40.0 70-71 32.623433120753006 35.0 32.0 38.0 23.5 40.0 72-73 32.12896998748495 35.0 31.5 37.0 22.5 39.0 74-75 31.615349422472967 35.0 31.0 36.5 20.5 39.0 76-77 30.374446449557432 33.5 29.5 35.0 20.0 37.0 78-79 30.98810320650565 34.0 31.0 36.0 20.0 37.0 80-81 30.818678399453624 34.0 31.0 35.0 20.0 37.0 82-83 30.485620176300593 34.0 30.5 35.0 18.5 36.5 84-85 30.127114072260355 34.0 30.0 35.0 17.5 36.0 86-87 29.733215478705336 34.0 30.0 35.0 12.0 36.0 88-89 29.389820529773658 34.0 29.5 35.0 7.0 35.0 90-91 28.883782561390063 34.0 29.0 35.0 2.0 35.0 92-93 28.419366227882957 33.0 29.0 35.0 2.0 35.0 94-95 27.947054421410368 33.0 27.5 35.0 2.0 35.0 96-97 27.38987933599377 33.0 27.0 35.0 2.0 35.0 98-99 26.512715766606057 32.5 25.0 34.0 2.0 35.0 100-101 23.872822353447635 30.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2720491808882315 1101 2 0.2104151045574838 1101 3 0.16780516915292765 1101 4 0.14663607191089056 1101 5 0.12338019572550962 1101 6 0.18500676652560344 1101 7 0.14340397461925392 1101 8 0.19519925075000089 1101 9 0.27114069225810766 1101 10-11 0.21703872023356752 1101 12-13 0.26573839228085205 1101 14-15 0.21261380311234745 1101 16-17 0.2264549213906406 1101 18-19 0.3366723191861354 1101 20-21 0.26213924690924983 1101 22-23 0.25930457986527244 1101 24-25 0.2601910357505304 1101 26-27 0.24587830074167272 1101 28-29 0.2102817932341381 1101 30-31 0.3605707536222411 1101 32-33 0.3907049220530183 1101 34-35 0.2740388416287445 1101 36-37 0.217179563944903 1101 38-39 0.18534416348685312 1101 40-41 0.223760625881674 1101 42-43 0.2860325028753721 1101 44-45 0.29886581214487506 1101 46-47 0.3170196118845183 1101 48-49 0.32388536742896434 1101 50-51 0.2758346355167518 1101 52-53 0.15253074261578803 1101 54-55 0.2827710645653241 1101 56-57 0.2361780865779295 1101 58-59 0.28195227599484696 1101 60-61 0.16597352889094452 1101 62-63 0.34638471408361937 1101 64-65 0.3453155662335945 1101 66-67 0.2244104686784567 1101 68-69 0.1916801342342751 1101 70-71 0.2606825515495288 1101 72-73 0.21877492527703168 1101 74-75 0.12975837893258912 1101 76-77 0.03412064522550651 1101 78-79 0.13337613226218537 1101 80-81 0.0209883220227276 1101 82-83 0.13064042516808527 1101 84-85 0.06390983463739985 1101 86-87 -0.07560295212696388 1101 88-89 0.2864687715832588 1101 90-91 0.26233743891475214 1101 92-93 0.2912072788105249 1101 94-95 0.13271607174976907 1101 96-97 0.39047285606977056 1101 98-99 0.46924671973234666 1101 100-101 0.8279602715683332 1102 1 0.2300643142546761 1102 2 0.1579109414209583 1102 3 0.10370192336718986 1102 4 0.07616907024563346 1102 5 0.0474955657333993 1102 6 0.09725033192336952 1102 7 0.12903852427012907 1102 8 0.09347892587927475 1102 9 0.1574023417366206 1102 10-11 0.10439469031742021 1102 12-13 0.1227925714856184 1102 14-15 0.17997196432067852 1102 16-17 0.11934559888633345 1102 18-19 0.14185688490525195 1102 20-21 0.14356260137476085 1102 22-23 0.11095559216477824 1102 24-25 0.05107486233032432 1102 26-27 0.09067116644034456 1102 28-29 0.11690293320317124 1102 30-31 0.1015233084733893 1102 32-33 0.051663685090211686 1102 34-35 0.05567239796985746 1102 36-37 0.099969960976658 1102 38-39 0.1746191934322141 1102 40-41 0.08506018181378039 1102 42-43 0.24802869031876895 1102 44-45 0.03642197796917657 1102 46-47 -0.05484357430263742 1102 48-49 -0.10103414526495413 1102 50-51 -0.18407341029234914 1102 52-53 -0.25028865029665326 1102 54-55 -0.11650842066649147 1102 56-57 -0.33915321144421995 1102 58-59 -0.2891438705475622 1102 60-61 -0.24130538664851287 1102 62-63 -0.22751059398849094 1102 64-65 -0.0897213907327199 1102 66-67 0.03817613521852081 1102 68-69 -0.11481589252665714 1102 70-71 -0.2579821041517718 1102 72-73 -0.13545803507822285 1102 74-75 -0.2123984179355638 1102 76-77 -0.3559469874835983 1102 78-79 -0.15697977939167274 1102 80-81 -0.10793956076672018 1102 82-83 -0.24840066571976038 1102 84-85 -0.2558164939144092 1102 86-87 -0.15349830402155717 1102 88-89 -0.09329612075949356 1102 90-91 -0.05321939885439164 1102 92-93 -0.11760228325672628 1102 94-95 -0.14980686201984383 1102 96-97 0.10436400100535437 1102 98-99 0.13112845744013768 1102 100-101 0.46348665270712885 1103 1 0.20840896754799587 1103 2 0.2144952093091277 1103 3 0.18012113044348155 1103 4 0.13337725220740992 1103 5 0.10621377770623042 1103 6 0.10204973170223042 1103 7 0.1638963666331108 1103 8 0.11066657396015245 1103 9 0.22255002772620003 1103 10-11 0.14075663945656913 1103 12-13 0.205592682052675 1103 14-15 0.2835736860076139 1103 16-17 0.309245298775771 1103 18-19 0.24545228847499345 1103 20-21 0.16324985457330854 1103 22-23 0.18138088042888256 1103 24-25 0.16852273786341954 1103 26-27 0.03264162924243408 1103 28-29 0.06769032855935109 1103 30-31 0.1847431789519831 1103 32-33 0.21224771604898507 1103 34-35 0.13871378163752723 1103 36-37 0.012058493593940511 1103 38-39 0.01930328757216415 1103 40-41 -0.0644229172227071 1103 42-43 -0.024998082702872182 1103 44-45 0.10682317841145306 1103 46-47 0.18167435335492144 1103 48-49 0.1814852039689825 1103 50-51 0.29695762745839005 1103 52-53 0.1498685128687285 1103 54-55 0.1820482844353819 1103 56-57 0.19935334360237533 1103 58-59 0.18191125502502103 1103 60-61 0.1877184642439218 1103 62-63 0.2867268882418088 1103 64-65 0.3171527207820475 1103 66-67 0.2908352727955261 1103 68-69 0.1640259176139196 1103 70-71 0.44500754993131153 1103 72-73 0.39720031273413525 1103 74-75 0.3641953271284102 1103 76-77 0.23348201267435442 1103 78-79 0.4532725504140416 1103 80-81 0.2822717012771925 1103 82-83 0.45608559237533086 1103 84-85 0.4956425962767099 1103 86-87 0.4832605018542928 1103 88-89 0.39372449209787774 1103 90-91 0.36000205115350425 1103 92-93 0.4530433493447603 1103 94-95 0.5578258184413798 1103 96-97 0.5542107866632264 1103 98-99 0.5915691461149137 1103 100-101 1.0272257934431899 1104 1 0.2058132646567259 1104 2 0.2696952579370091 1104 3 0.08337387953313424 1104 4 0.11120464983718392 1104 5 0.17753434748818364 1104 6 0.13264882334868844 1104 7 0.116746131186801 1104 8 0.1905219060258645 1104 9 0.2180335236906572 1104 10-11 0.19894549797413674 1104 12-13 0.22991364883565524 1104 14-15 0.27722575354717804 1104 16-17 0.3123801822493206 1104 18-19 0.19517201334758028 1104 20-21 0.13630934584212895 1104 22-23 0.15674563699506194 1104 24-25 0.09385843167495267 1104 26-27 0.1318613037775691 1104 28-29 0.09533138264457364 1104 30-31 0.2058458730503503 1104 32-33 0.20890217509234077 1104 34-35 0.1777819119526356 1104 36-37 0.21358609276347096 1104 38-39 0.12902287947790825 1104 40-41 0.2335991058869311 1104 42-43 0.24404039600438665 1104 44-45 0.22601352425822796 1104 46-47 0.23088082618124872 1104 48-49 0.1738708572256229 1104 50-51 0.2676787066933386 1104 52-53 0.24624127213984082 1104 54-55 0.14981816440430862 1104 56-57 0.3151985283978078 1104 58-59 0.23267353498862775 1104 60-61 0.236919903325159 1104 62-63 0.2904152937223188 1104 64-65 0.21123996983086357 1104 66-67 0.22803274946458885 1104 68-69 0.2469034912348107 1104 70-71 0.3571877883978587 1104 72-73 0.22048233352634128 1104 74-75 0.1847619748631253 1104 76-77 0.22529011491446482 1104 78-79 0.3027990934468612 1104 80-81 0.3111085072614621 1104 82-83 0.4209409339386774 1104 84-85 0.2872179115506128 1104 86-87 0.33246270074610607 1104 88-89 0.3472277887260269 1104 90-91 0.4983436126022376 1104 92-93 0.5134104898166392 1104 94-95 0.6172690292489236 1104 96-97 0.6500332531223378 1104 98-99 0.6168005559244456 1104 100-101 1.1004928079764689 1105 1 0.17534182155750244 1105 2 0.13930821503342372 1105 3 0.11535723008578458 1105 4 0.08147092029432201 1105 5 0.0018918656360256136 1105 6 -0.061615296119477136 1105 7 6.578814549840217E-5 1105 8 0.03501251886856949 1105 9 0.13349776530039748 1105 10-11 0.07311669615967276 1105 12-13 0.14419227937266754 1105 14-15 0.07650799158454902 1105 16-17 0.17015864659811797 1105 18-19 0.19761394430155832 1105 20-21 0.2303493589210106 1105 22-23 0.10950719878601234 1105 24-25 0.13166152250559549 1105 26-27 -0.04603282576997003 1105 28-29 0.07863126815935573 1105 30-31 0.1131153240527496 1105 32-33 0.10368656727131764 1105 34-35 0.17231796759011075 1105 36-37 0.008394985319405635 1105 38-39 0.04912260141663438 1105 40-41 0.08567605328408234 1105 42-43 0.16642937191565466 1105 44-45 0.10552616492146427 1105 46-47 -0.0014696697261982195 1105 48-49 0.019131385406915058 1105 50-51 0.08406436964921937 1105 52-53 0.2047892464901011 1105 54-55 0.22094289384178012 1105 56-57 0.06926451007476686 1105 58-59 0.14211221513890848 1105 60-61 0.10849987138459483 1105 62-63 0.02306146053926028 1105 64-65 0.07721142328870201 1105 66-67 0.05983631048628979 1105 68-69 -0.14202377957631285 1105 70-71 -0.12680634952665315 1105 72-73 0.02814469130931485 1105 74-75 0.036535369795693384 1105 76-77 -0.05397522506879682 1105 78-79 0.0660752352139724 1105 80-81 -0.026812491633318558 1105 82-83 0.19896301490341628 1105 84-85 0.1606757164069137 1105 86-87 0.3174267203211869 1105 88-89 0.13189131838364432 1105 90-91 -0.13254267051943813 1105 92-93 0.13748778490296232 1105 94-95 0.3149764875419869 1105 96-97 0.34132602632180564 1105 98-99 0.5861917485793775 1105 100-101 0.8091560782183294 1106 1 0.09836547417260277 1106 2 0.05587103521937564 1106 3 0.04126358326417545 1106 4 0.04207648618299231 1106 5 0.011441589769241034 1106 6 0.06412923146693572 1106 7 0.05530354667288151 1106 8 0.15563566135936924 1106 9 0.0770183544664107 1106 10-11 -0.014219165886260043 1106 12-13 -0.08554533312473467 1106 14-15 0.12135748000260094 1106 16-17 0.24929430287695453 1106 18-19 0.260435985445433 1106 20-21 0.1622443986194284 1106 22-23 0.09601850829057668 1106 24-25 0.23728094666515176 1106 26-27 0.2014104570898212 1106 28-29 0.1774567207330051 1106 30-31 0.11604175409961925 1106 32-33 0.015129725038285358 1106 34-35 0.006339664834733583 1106 36-37 -0.030432555668369332 1106 38-39 -0.07562807427013496 1106 40-41 -0.009004241786570333 1106 42-43 -0.2905860033707768 1106 44-45 -0.1344289076721239 1106 46-47 -0.08118480791931404 1106 48-49 -0.132283674179142 1106 50-51 -0.05920918768861583 1106 52-53 -0.1314140219310076 1106 54-55 -0.10923537933656746 1106 56-57 -0.24234675442818343 1106 58-59 -0.38717368504444494 1106 60-61 -0.4477979958357281 1106 62-63 -0.3580531801448501 1106 64-65 -0.3190744329001376 1106 66-67 -0.31615429483046853 1106 68-69 -0.28671311207357775 1106 70-71 -0.1490083383965768 1106 72-73 -0.17843948476625826 1106 74-75 -0.2529341995192489 1106 76-77 -0.2261105065992126 1106 78-79 -0.23442701778202846 1106 80-81 -0.2855827708500449 1106 82-83 -0.3217925881685666 1106 84-85 -0.20506642063439529 1106 86-87 -0.18308584223185065 1106 88-89 -0.3757674368685322 1106 90-91 -0.12556331242585017 1106 92-93 -0.13410358135982037 1106 94-95 0.05023700130246311 1106 96-97 0.008684590595404273 1106 98-99 0.29894178184028775 1106 100-101 0.26208208422172774 1107 1 0.3012363598746717 1107 2 0.29806745682984115 1107 3 0.30625227522353526 1107 4 0.1598951076152062 1107 5 0.11218738810660511 1107 6 0.2107264299578233 1107 7 0.23404580405659203 1107 8 0.16714187176619788 1107 9 0.25828804908920944 1107 10-11 0.2994887208925121 1107 12-13 0.296581093688971 1107 14-15 0.34906381095984784 1107 16-17 0.3755710296070305 1107 18-19 0.2783013158686174 1107 20-21 0.39562278094282277 1107 22-23 0.407773718833063 1107 24-25 0.4150854180206949 1107 26-27 0.45552557071115984 1107 28-29 0.4142543042296225 1107 30-31 0.4214735632659128 1107 32-33 0.3845762063229188 1107 34-35 0.3556202261668986 1107 36-37 0.3286177406417963 1107 38-39 0.41532411942105085 1107 40-41 0.3057705676424618 1107 42-43 0.30635009289096615 1107 44-45 0.24688303111162213 1107 46-47 -0.11644391402600718 1107 48-49 3.5629394883329724E-4 1107 50-51 0.09481096243271026 1107 52-53 0.015798464481314056 1107 54-55 0.09482074701125498 1107 56-57 -0.04761353660801859 1107 58-59 -0.23579516877614282 1107 60-61 -0.015110288371388947 1107 62-63 0.21192461591201806 1107 64-65 0.18334825255774945 1107 66-67 0.12838118253714015 1107 68-69 0.03296923244300842 1107 70-71 0.12784718485574587 1107 72-73 0.3019988928518913 1107 74-75 0.2454673913340315 1107 76-77 0.12498603848097645 1107 78-79 0.19348916018609685 1107 80-81 0.2070088740791931 1107 82-83 0.30552495912857935 1107 84-85 0.37819527507370054 1107 86-87 0.4380619968762858 1107 88-89 0.32934462177872703 1107 90-91 0.35370974455740267 1107 92-93 0.44685597930090637 1107 94-95 0.6565380785285697 1107 96-97 0.6791468207093061 1107 98-99 1.1021185083986431 1107 100-101 1.2358616830395768 1108 1 0.1260493493856174 1108 2 0.09729546555637114 1108 3 0.005379996184053937 1108 4 0.017410515632988677 1108 5 -0.06649248062119995 1108 6 -0.06600680832665518 1108 7 0.023259256088728364 1108 8 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-0.10156793566505229 2316 64-65 -0.14335752551892256 2316 66-67 -0.3557158703915775 2316 68-69 -0.06596641217998211 2316 70-71 -0.108094755642405 2316 72-73 -0.11149572343007463 2316 74-75 -0.2011834360964606 2316 76-77 -0.12719613338211389 2316 78-79 -0.15765667729508337 2316 80-81 -0.3133008906476853 2316 82-83 -0.33113549867548997 2316 84-85 -0.07378544058251535 2316 86-87 -0.16985245945004834 2316 88-89 -0.02724377782425691 2316 90-91 0.051311294855825196 2316 92-93 -0.04273525337216455 2316 94-95 0.03290369647262281 2316 96-97 0.07042506476627253 2316 98-99 0.21273920293587523 2316 100-101 0.6943674561019613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 10.0 5 36.0 6 63.0 7 212.0 8 538.0 9 1026.0 10 1725.0 11 2791.0 12 4333.0 13 6116.0 14 7803.0 15 10784.0 16 14909.0 17 20831.0 18 19075.0 19 11776.0 20 10236.0 21 10712.0 22 11485.0 23 12650.0 24 14302.0 25 17079.0 26 21133.0 27 26460.0 28 33013.0 29 41522.0 30 52808.0 31 66452.0 32 85411.0 33 112959.0 34 155571.0 35 225575.0 36 347115.0 37 502358.0 38 331575.0 39 21695.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.1504874278896 19.583368723312738 15.305957916280406 22.960185932517252 2 15.845665232309544 20.873846807753157 38.634869227681335 24.64561873225596 3 19.041090789538213 24.08453019506409 30.437009683272876 26.437369332124817 4 13.706330606141382 16.984546416831243 35.811025896138446 33.49809708088893 5 14.152368970270631 37.029197958845344 33.98813187112656 14.830301199757459 6 32.91908627394707 35.820934832717874 16.587622630268264 14.672356263066789 7 28.656520815131408 32.86268679489299 20.40362409792621 18.0771682920494 8 26.66614596294164 34.61983481582527 19.626201327067935 19.087817894165152 9 26.250824195285695 16.697787796278906 20.34522656969468 36.70616143874072 10-11 25.034455011404777 26.58707615794949 26.8590380206226 21.519430810023128 12-13 25.798669397109876 23.967404520580388 27.657067655668982 22.576858426640758 14-15 23.06068892735638 25.276389234511033 25.887451706243176 25.77547013188941 16-17 22.32474838203427 28.67184676052654 26.600687147276204 22.402717710162985 18-19 22.307674143608878 27.010900266467104 29.052634064558784 21.628791525365234 20-21 22.67883448342255 26.02232002572034 29.44581835553287 21.853027135324236 22-23 22.9380034752693 25.746464277905297 29.191535564520105 22.123996682305304 24-25 22.819520490793646 26.056519828939706 29.13442446520953 21.98953521505712 26-27 22.47799127984003 26.189928562622196 29.226000090366703 22.10608006717107 28-29 22.42886867406195 26.357689212778208 29.240451491378323 21.972990621781516 30-31 22.466064951129532 26.172196419132593 29.077927550736828 22.283811079001048 32-33 22.512655825130736 26.21983172793109 29.0744536697806 22.193058777157574 34-35 23.0130082083493 26.000772881750002 28.91492760704548 22.07129130285521 36-37 22.84083540252517 25.870332057609208 28.83566408797229 22.453168451893333 38-39 22.58104652939599 26.185758388807646 28.948632140791787 22.28456294100458 40-41 22.912250977856296 26.214881579882466 28.783702750757957 22.08916469150328 42-43 22.66146347223531 26.18392921415036 28.48851137848698 22.666095935127352 44-45 22.847419882769003 26.331154854260475 28.38796484160011 22.43346042137041 46-47 22.925570851732036 26.68517284033589 27.82683089419966 22.56242541373241 48-49 23.034101250233864 26.78843565464265 27.55988698307289 22.617576112050596 50-51 23.03762054139867 26.931727567811066 27.508936729048184 22.52171516174208 52-53 23.204321417089133 26.992145850324317 27.13911599038757 22.66441674219898 54-55 23.111866232423978 26.89390086406545 27.318418198958472 22.6758147045521 56-57 23.096721927863157 27.26608293357213 27.248895169625293 22.388299968939418 58-59 23.159160964658142 26.819609762831565 27.397227615945884 22.62400165656441 60-61 23.089706050637833 26.776356539160854 27.64998083689198 22.48395657330933 62-63 22.737322832071232 27.01512341586487 27.840295021043094 22.407258731020804 64-65 23.052310743873708 27.00211339110723 27.489614685298175 22.45596117972089 66-67 22.871742242515545 27.104269809356545 27.28838554184041 22.7356024062875 68-69 22.75786891514005 26.91517728615632 27.560547756597426 22.766406042106198 70-71 22.875801263130363 26.94421537517006 27.446611217774645 22.73337214392493 72-73 22.947662918205317 26.650502146086456 27.42460997171852 22.977224963989702 74-75 22.893556654684428 27.35513235259392 26.841111496593328 22.910199496128325 76-77 23.097312260574675 26.95368340365861 27.192995202865568 22.756009132901152 78-79 23.010510646877503 26.90078051066504 27.130964857947788 22.95774398450967 80-81 22.821773478337278 27.23133378471101 27.026896932856886 22.91999580409482 82-83 22.94774437669369 27.134279321059484 27.0707719763908 22.847204325856026 84-85 22.819252450059384 27.1269703272793 27.004975386447867 23.048801836213446 86-87 22.839087109494912 27.030290425529984 27.12560645333556 23.005016011639544 88-89 22.92902202758398 26.94762114081342 26.974254228144524 23.149102603458076 90-91 22.78315335518685 26.89944385254054 27.070656977002944 23.24674581526967 92-93 22.66217457970128 26.878592176675433 27.1481537236481 23.311079519975188 94-95 22.667368405756562 26.840839035341858 27.157143843193097 23.334648715708482 96-97 22.694387479860573 26.693210447254227 27.115979489116988 23.49642258376821 98-99 22.379472312684353 26.736594783614052 26.96187784626486 23.922055057436737 100-101 22.32274751989027 26.738245523198977 27.269228017944673 23.66977893896608 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 317.0 1 242.5 2 224.5 3 307.5 4 310.5 5 338.5 6 575.5 7 1189.0 8 2027.0 9 2604.5 10 2641.0 11 2632.5 12 2817.5 13 2984.0 14 3217.5 15 3471.0 16 3630.0 17 3624.0 18 3714.5 19 3929.0 20 4088.5 21 4456.0 22 5150.5 23 6050.0 24 7125.0 25 8649.0 26 10726.5 27 12732.5 28 14016.0 29 15881.5 30 19017.5 31 22357.5 32 26330.5 33 29602.0 34 33701.0 35 38059.5 36 43687.5 37 50645.0 38 54656.0 39 57143.5 40 58849.5 41 63624.5 42 68313.5 43 71935.0 44 77964.5 45 82173.0 46 84673.5 47 89703.5 48 95994.0 49 96947.0 50 94893.0 51 90842.0 52 85863.5 53 85230.5 54 84307.5 55 78050.0 56 69062.0 57 60003.0 58 52886.5 59 46872.5 60 38933.0 61 30872.0 62 24201.5 63 18298.0 64 13573.5 65 9985.0 66 7052.0 67 5228.0 68 3711.5 69 2443.5 70 1695.5 71 1104.5 72 705.0 73 483.5 74 326.0 75 223.5 76 157.5 77 89.5 78 53.0 79 34.5 80 20.5 81 13.0 82 6.5 83 6.5 84 5.0 85 5.0 86 5.5 87 5.0 88 3.0 89 1.5 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02220559199472515 2 0.0 3 0.0 4 2.270510428908502E-4 5 0.020570824485911028 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.541020857817004E-5 12-13 0.0028835482447137975 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.492684415398057E-4 24-25 2.270510428908502E-5 26-27 4.313969814926154E-4 28-29 0.0010898450058760812 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.135255214454251E-4 40-41 4.313969814926154E-4 42-43 0.013418716634849248 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.270510428908502E-5 68-69 9.082041715634008E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.541020857817004E-5 82-83 0.0018391134474158868 84-85 3.405765643362753E-4 86-87 0.0 88-89 0.0 90-91 0.00971778463572839 92-93 0.0024294461589320976 94-95 0.0 96-97 0.0 98-99 0.0013396011530560164 100-101 0.0172785843639937 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2202148.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.81964301402077 #Duplication Level Percentage of deduplicated Percentage of total 1 63.3448020831504 23.323329994953966 2 15.830947534816106 11.657796736112422 3 7.126391549028027 7.871735784400393 4 3.843062213459109 5.6600071512094745 5 2.3794858985329315 4.380591067043949 6 1.5524982024555065 3.4297457756592418 7 1.0873687524525857 2.8025570502933728 8 0.7936518854581042 2.3377583279977516 9 0.5748728127509759 1.904995056956125 >10 3.1184597695610656 20.60791625162852 >50 0.2213423860378398 5.669162088180771 >100 0.12143611772613479 7.912860053118388 >500 0.004939821366201878 1.1872377527973617 >1k 3.7048660246514033E-4 0.22698930915221838 >5k 3.7048660246514033E-4 1.0273176004960076 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9414 0.4274917035548928 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8065 0.36623333218294135 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5114 0.2322278066687616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.541020857817004E-5 0.0 18-19 0.0 0.0 0.0 6.811531286725506E-5 0.0 20-21 0.0 0.0 0.0 1.3623062573451012E-4 0.0 22-23 0.0 0.0 0.0 3.178714600471903E-4 0.0 24-25 0.0 0.0 0.0 5.449225029380405E-4 0.0 26-27 0.0 0.0 0.0 9.763194844306559E-4 0.0 28-29 0.0 0.0 0.0 0.0025429716803775223 0.0 30-31 0.0 0.0 0.0 0.005994147532318445 0.0 32-33 0.0 0.0 0.0 0.010240002034377345 0.0 34-35 0.0 0.0 0.0 0.015552996438023239 0.0 36-37 0.0 0.0 0.0 0.02075246532022371 0.0 38-39 0.0 0.0 0.0 0.026337920975338623 0.0 40-41 0.0 0.0 0.0 0.03462528404085466 0.0 42-43 0.0 0.0 0.0 0.04443388909373938 0.0 44-45 0.0 0.0 0.0 0.05499176258816392 0.0 46-47 0.0 0.0 0.0 0.06741145463429343 0.0 48-49 0.0 0.0 0.0 0.07910458334317222 0.0 50-51 0.0 0.0 0.0 0.09520250228413349 0.0 52-53 0.0 0.0 0.0 0.1361171002130647 0.0 54-55 0.0 0.0 0.0 0.1946281539660368 0.0 56-57 0.0 0.0 0.0 0.24017459316994133 0.0 58-59 0.0 0.0 0.0 0.3107420573004176 4.541020857817004E-5 60-61 0.0 0.0 0.0 0.392026330655342 4.541020857817004E-5 62-63 0.0 0.0 0.0 0.4491069628381017 4.541020857817004E-5 64-65 0.0 0.0 0.0 0.5011243567643955 4.541020857817004E-5 66-67 0.0 0.0 0.0 0.6251396363913779 4.541020857817004E-5 68-69 0.0 0.0 0.0 0.7478380199695933 4.541020857817004E-5 70-71 0.0 0.0 0.0 0.8127973233406656 4.541020857817004E-5 72-73 0.0 0.0 0.0 0.9469390794805799 4.541020857817004E-5 74-75 0.0 0.0 0.0 1.1337339724668825 4.541020857817004E-5 76-77 0.0 0.0 0.0 1.3217322359805064 4.541020857817004E-5 78-79 0.0 0.0 0.0 1.514475866290549 4.541020857817004E-5 80-81 0.0 0.0 0.0 1.7566258035336408 4.541020857817004E-5 82-83 0.0 0.0 0.0 2.017552862023806 4.541020857817004E-5 84-85 0.0 0.0 0.0 2.3053854690965365 4.541020857817004E-5 86-87 0.0 0.0 4.541020857817004E-5 2.682199379878192 4.541020857817004E-5 88-89 0.0 0.0 4.541020857817004E-5 3.1073070474827302 4.541020857817004E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6780 0.0 35.881767 1 GTATCAA 10190 0.0 31.661327 1 ATCAACG 12525 0.0 25.678259 3 TCAACGC 12705 0.0 25.314459 4 TATCAAC 13000 0.0 24.88619 2 CAACGCA 12970 0.0 24.833868 5 AACGCAG 13300 0.0 24.249004 6 GTACATG 6735 0.0 24.128042 1 TACATGG 6760 0.0 22.9803 2 GTATACG 105 0.0021950626 22.622227 4 GAGTACT 8360 0.0 22.328629 12-13 ACATGGG 6965 0.0 21.962885 3 AGTACTT 8890 0.0 21.50502 12-13 ACGCAGA 14990 0.0 21.356695 7 CGCAGAG 14895 0.0 21.205908 8 GTACTTT 8805 0.0 21.17318 14-15 CCCTATA 225 7.1486284E-8 21.114079 2 AGAGTAC 12325 0.0 20.810503 10-11 GCAGAGT 15845 0.0 19.514816 9 CATGGGG 4505 0.0 19.29794 4 >>END_MODULE