FastQCFastQC Report
Thu 26 May 2016
SRR937765_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937765_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057274
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74820.3636851484051225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60490.29402986670710857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35630.17319034800420363No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26800.13026947309886772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.037.609371
GTATCAA84350.032.753711
ATCAACG101700.026.9650973
TCAACGC103150.026.581514
TATCAAC104600.026.3128642
CAACGCA104700.026.1886275
AACGCAG106750.025.7628146
GTACATG63250.024.884961
TACATGG63600.023.9174082
GAGTACT64100.023.78384212-13
ACATGGG65600.022.2424013
CGCAGAG121050.022.2092678
ACGCAGA122900.022.0295477
GTACTTT71650.021.17848814-15
GCAGAGT127650.020.5767299
AGTACTT67800.020.3493912-13
AGAGTAC98750.020.20054210-11
CATGGGG40450.019.7362124
CAGAGTA120750.018.48679410-11
GAGTACA52300.018.093431
Produced by FastQC (version 0.11.5)