##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937765_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2057274 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.808681293789743 33.0 31.0 34.0 30.0 34.0 2 31.954713859213697 33.0 31.0 34.0 30.0 34.0 3 31.975194359137383 34.0 31.0 34.0 30.0 34.0 4 35.57984789580775 37.0 35.0 37.0 33.0 37.0 5 35.56529660123056 37.0 35.0 37.0 33.0 37.0 6 35.61219798626726 37.0 35.0 37.0 33.0 37.0 7 35.55310522565298 37.0 35.0 37.0 33.0 37.0 8 35.53447523275947 37.0 35.0 37.0 33.0 37.0 9 37.17972812566532 39.0 37.0 39.0 34.0 39.0 10-11 37.065193066164255 39.0 37.0 39.0 33.0 39.0 12-13 36.950541590473605 39.0 37.0 39.0 33.0 39.0 14-15 38.13242742580716 40.0 38.0 41.0 33.0 41.0 16-17 38.0549732315676 40.0 38.0 41.0 33.0 41.0 18-19 37.98072400662235 40.0 38.0 41.0 33.0 41.0 20-21 37.888706608842575 40.0 38.0 41.0 33.0 41.0 22-23 37.69571165532642 40.0 37.5 41.0 32.5 41.0 24-25 37.522626300628886 40.0 37.0 41.0 32.0 41.0 26-27 37.47795091951777 39.5 37.0 41.0 32.0 41.0 28-29 37.52675919687898 40.0 37.0 41.0 32.0 41.0 30-31 37.36327805630169 40.0 37.0 41.0 32.0 41.0 32-33 37.14592198219586 39.0 36.5 41.0 31.0 41.0 34-35 37.196335539164934 39.5 37.0 41.0 31.0 41.0 36-37 37.134319492687894 40.0 36.5 41.0 31.0 41.0 38-39 37.021373672150624 40.0 36.0 41.0 31.0 41.0 40-41 36.81139216263852 39.5 36.0 41.0 30.5 41.0 42-43 36.573662283196114 39.0 36.0 41.0 30.0 41.0 44-45 36.358145293237556 39.0 35.5 41.0 30.0 41.0 46-47 36.03655395440762 39.0 35.0 41.0 29.5 41.0 48-49 35.70798979620605 39.0 35.0 40.0 28.0 41.0 50-51 34.76123355469422 38.0 34.0 39.5 26.5 40.5 52-53 34.85521957697419 38.0 34.5 39.5 26.5 40.5 54-55 35.212883164809355 38.0 35.0 40.0 27.0 41.0 56-57 35.00170857163411 38.0 34.5 40.0 26.0 41.0 58-59 34.746540081680905 38.0 34.0 40.0 26.0 41.0 60-61 34.41864015196809 38.0 34.0 40.0 25.5 41.0 62-63 34.05169656545506 37.0 33.5 40.0 24.5 41.0 64-65 33.65824775892759 37.0 33.0 39.5 23.5 41.0 66-67 33.225627942607545 36.0 32.5 39.0 22.5 41.0 68-69 32.73158898620213 35.5 32.0 39.0 21.5 40.0 70-71 32.27382691853394 35.0 32.0 38.0 20.0 40.0 72-73 31.811745056808185 35.0 31.0 37.0 18.5 39.5 74-75 31.351188271469915 35.0 31.0 37.0 17.5 39.0 76-77 30.866789256073815 34.5 30.5 36.0 14.0 39.0 78-79 30.35879518236268 34.0 30.0 36.0 8.5 37.5 80-81 29.851760144735216 34.0 29.5 35.0 7.0 37.0 82-83 29.355235131538144 34.0 29.0 35.0 2.0 36.5 84-85 28.913757963207622 34.0 29.0 35.0 2.0 36.0 86-87 28.472877944308827 34.0 28.5 35.0 2.0 36.0 88-89 28.020875196983972 33.0 27.0 35.0 2.0 35.0 90-91 27.54471669792162 33.0 26.5 35.0 2.0 35.0 92-93 26.991300624029662 33.0 25.5 35.0 2.0 35.0 94-95 26.36457102943021 32.0 24.5 34.5 2.0 35.0 96-97 25.64437430308262 32.0 23.5 34.0 2.0 35.0 98-99 24.699598108953886 32.0 13.0 34.0 2.0 35.0 100-101 22.276380540462767 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15872995501243636 1101 2 0.2071323591563896 1101 3 0.25830069771958364 1101 4 0.15361365457754061 1101 5 0.11386426201824662 1101 6 0.12313752968498193 1101 7 0.11045479058905272 1101 8 7.124738705073241E-4 1101 9 0.11695092498996473 1101 10-11 0.2041680070075742 1101 12-13 0.29962075530245613 1101 14-15 0.3957549881621176 1101 16-17 0.388828851883396 1101 18-19 0.3830618947484581 1101 20-21 0.44305677088018314 1101 22-23 0.4158022732804909 1101 24-25 0.32242389541178085 1101 26-27 0.3174251234566299 1101 28-29 0.25642596962615016 1101 30-31 0.5110441417003955 1101 32-33 0.4078043391404904 1101 34-35 0.4707181558184459 1101 36-37 0.5753197353194892 1101 38-39 0.609824444367014 1101 40-41 0.5461041983820181 1101 42-43 0.6110206173791894 1101 44-45 0.5753129918483069 1101 46-47 0.5735921330456932 1101 48-49 0.6608366472255014 1101 50-51 0.45976143152688564 1101 52-53 0.5221006502156698 1101 54-55 0.5378515903172811 1101 56-57 0.5030573852808473 1101 58-59 0.5467097705969977 1101 60-61 0.4909616701124335 1101 62-63 0.5813064661894245 1101 64-65 0.5225338770735064 1101 66-67 0.6492297333038408 1101 68-69 0.6292062264018341 1101 70-71 0.6694186914266353 1101 72-73 0.6515104284298836 1101 74-75 0.6534060132389676 1101 76-77 0.7672098913411141 1101 78-79 0.8802755990158033 1101 80-81 0.765972171410418 1101 82-83 0.9220256420503326 1101 84-85 1.2329788066702072 1101 86-87 1.0275054232328102 1101 88-89 0.9985932258081327 1101 90-91 1.216871475204929 1101 92-93 1.1937653524976852 1101 94-95 1.44857477224717 1101 96-97 1.3918681676969342 1101 98-99 1.690965212833646 1101 100-101 2.138836175191635 1102 1 0.3554779201992204 1102 2 0.27673129084420367 1102 3 0.3065559476222788 1102 4 0.28749463422094834 1102 5 0.27283281054635466 1102 6 0.21590202720033602 1102 7 0.2172556919422064 1102 8 0.3369642999849276 1102 9 0.3756845463652354 1102 10-11 0.2843139833512893 1102 12-13 0.4347782523834951 1102 14-15 0.5660045524328865 1102 16-17 0.5330654514468165 1102 18-19 0.5480069138979786 1102 20-21 0.5101858651838356 1102 22-23 0.56608091436933 1102 24-25 0.6281968847117341 1102 26-27 0.5426562432666628 1102 28-29 0.44530542633243186 1102 30-31 0.7273734824942437 1102 32-33 0.668580881960402 1102 34-35 0.6188340146703837 1102 36-37 0.7462401674951948 1102 38-39 0.7974453627545017 1102 40-41 0.7693449239769521 1102 42-43 0.8255076272895607 1102 44-45 0.9337947645471232 1102 46-47 0.9513503413947717 1102 48-49 0.9511007076015048 1102 50-51 0.9038770421964699 1102 52-53 0.9230984560581135 1102 54-55 0.890668530454569 1102 56-57 1.0964878152567579 1102 58-59 0.881153813938667 1102 60-61 1.0030707829169572 1102 62-63 1.0072324965691948 1102 64-65 0.9868305198432168 1102 66-67 1.1722750858073852 1102 68-69 1.212636035921875 1102 70-71 1.3042092029642944 1102 72-73 1.3256374893304503 1102 74-75 1.3607718819617851 1102 76-77 1.3763526610561065 1102 78-79 1.5536581128773506 1102 80-81 1.5278595417492404 1102 82-83 1.465786817306551 1102 84-85 1.4928098593581929 1102 86-87 1.485979651752345 1102 88-89 1.5326150066292747 1102 90-91 1.536033620064451 1102 92-93 1.8029239861410602 1102 94-95 1.8945362391176488 1102 96-97 1.9665851552144886 1102 98-99 2.045841995939192 1102 100-101 2.6139805721931424 1103 1 0.3222129798936777 1103 2 0.3189952320370679 1103 3 0.32205461508389277 1103 4 0.24600059978533295 1103 5 0.12381034176765837 1103 6 0.18605518566178603 1103 7 0.25261182088130596 1103 8 0.28495551679858977 1103 9 0.26690508632081134 1103 10-11 0.34597260743262126 1103 12-13 0.34429703251893073 1103 14-15 0.3737883155709838 1103 16-17 0.47490158687588035 1103 18-19 0.35235520830318023 1103 20-21 0.29492628220274497 1103 22-23 0.5074186579273103 1103 24-25 0.461292181584831 1103 26-27 0.3039885448822446 1103 28-29 0.3751959741986255 1103 30-31 0.5456718003052288 1103 32-33 0.5047547384344071 1103 34-35 0.4612115690257639 1103 36-37 0.4219366934243851 1103 38-39 0.6376128558045764 1103 40-41 0.7895584212361868 1103 42-43 0.7235759124100838 1103 44-45 0.8205589863765326 1103 46-47 0.7758637106402659 1103 48-49 0.7599628642098892 1103 50-51 0.8506185918536104 1103 52-53 0.797565227216289 1103 54-55 0.9328589161493213 1103 56-57 0.8758941001179963 1103 58-59 0.9899142543556039 1103 60-61 1.0785810329435392 1103 62-63 1.108934423296276 1103 64-65 1.1699400426213558 1103 66-67 1.1254542941716679 1103 68-69 1.2041411522016716 1103 70-71 1.2025267810592695 1103 72-73 1.1226623538059037 1103 74-75 1.1473380861798042 1103 76-77 1.136309824197152 1103 78-79 1.1384850692013018 1103 80-81 1.0773133873238407 1103 82-83 1.2603569853701941 1103 84-85 1.3138262371982314 1103 86-87 1.4338051260979725 1103 88-89 1.341487643440562 1103 90-91 1.5463276052819346 1103 92-93 1.7747006056623427 1103 94-95 1.8755464996244378 1103 96-97 2.08940643747896 1103 98-99 2.0991184955203757 1103 100-101 2.3815183183905724 1104 1 0.33805673344527776 1104 2 0.42896917157997905 1104 3 0.3306742566336709 1104 4 0.2985728144748663 1104 5 0.24029014491341627 1104 6 0.1605953613187765 1104 7 0.2191587574560785 1104 8 0.2905587793775197 1104 9 0.45127885565140957 1104 10-11 0.5160986940558914 1104 12-13 0.4574604707019958 1104 14-15 0.5914146070305009 1104 16-17 0.6462091161278138 1104 18-19 0.6120773314110863 1104 20-21 0.696649483674399 1104 22-23 0.7342470527247329 1104 24-25 0.7148685564325277 1104 26-27 0.6971492607254675 1104 28-29 0.7241523665432936 1104 30-31 0.8944838161318032 1104 32-33 0.7296077849441289 1104 34-35 0.7920743128800112 1104 36-37 0.9470274821364555 1104 38-39 0.8993010765308327 1104 40-41 0.8804297285672078 1104 42-43 1.084675790565548 1104 44-45 1.1255165522346857 1104 46-47 1.0865186976761123 1104 48-49 0.924974652205691 1104 50-51 0.9601475081244857 1104 52-53 0.9704784083628866 1104 54-55 0.9783305813490912 1104 56-57 1.0611437096075917 1104 58-59 1.0604701006464836 1104 60-61 0.9846408160549913 1104 62-63 1.046716403520989 1104 64-65 0.9076042750404625 1104 66-67 1.1127124882824262 1104 68-69 1.1361920123817697 1104 70-71 1.0196489771153168 1104 72-73 0.9610442399380936 1104 74-75 0.9784757070662202 1104 76-77 1.204298609742846 1104 78-79 1.2407915253755917 1104 80-81 1.3226474157788992 1104 82-83 1.319921384565646 1104 84-85 1.305972088537331 1104 86-87 1.5430945161088374 1104 88-89 1.6160673326709336 1104 90-91 1.6218114152890415 1104 92-93 1.6085516460983378 1104 94-95 1.7709911396544626 1104 96-97 2.0538571063023525 1104 98-99 2.2306474857966023 1104 100-101 2.8908013650853093 1105 1 0.3096513234084526 1105 2 0.20965558254048489 1105 3 0.3385276707163172 1105 4 0.2620203754861521 1105 5 0.3175066759529983 1105 6 0.31256665602451505 1105 7 0.42969524720150076 1105 8 0.3277820395265607 1105 9 0.34043140581670883 1105 10-11 0.4061817212356331 1105 12-13 0.4202314636427005 1105 14-15 0.5455223939894012 1105 16-17 0.5445656042850047 1105 18-19 0.5815466094737758 1105 20-21 0.628148403255274 1105 22-23 0.6850106603633179 1105 24-25 0.6561492397088102 1105 26-27 0.4960659158983134 1105 28-29 0.6365778134439353 1105 30-31 0.6916816226928901 1105 32-33 0.6011309042416286 1105 34-35 0.6805461154036365 1105 36-37 0.6309923486618558 1105 38-39 0.6541227342323168 1105 40-41 0.8001579310590685 1105 42-43 0.929088644418492 1105 44-45 0.8284713000184709 1105 46-47 0.853930268400731 1105 48-49 0.8941947233419896 1105 50-51 0.790123078531451 1105 52-53 0.8309554437095201 1105 54-55 0.8406163260859714 1105 56-57 0.7760515082692976 1105 58-59 0.7404763909009731 1105 60-61 0.7126202233082211 1105 62-63 0.762368688283523 1105 64-65 0.7832541983453325 1105 66-67 0.8633159138588979 1105 68-69 0.918962984424617 1105 70-71 1.0047002186569074 1105 72-73 1.03764062711684 1105 74-75 1.1347430784085795 1105 76-77 1.0944997098059552 1105 78-79 0.959658945200971 1105 80-81 1.0738495649143545 1105 82-83 1.1562692335493878 1105 84-85 1.351881542309183 1105 86-87 1.4013501094198517 1105 88-89 1.3185665072056416 1105 90-91 1.3014237809680758 1105 92-93 1.5039840018299948 1105 94-95 1.6934486020696724 1105 96-97 1.8821770441103354 1105 98-99 1.9857319340305217 1105 100-101 2.3953257507928463 1106 1 0.13973923155828416 1106 2 0.16686348069951862 1106 3 0.2504830976464021 1106 4 0.12667019535317792 1106 5 0.08061620277475612 1106 6 -0.002668086970388117 1106 7 0.06523682710479761 1106 8 0.05693100284567976 1106 9 0.1370107797086959 1106 10-11 0.027100222941946583 1106 12-13 -0.08908434326344405 1106 14-15 -0.06533268787811863 1106 16-17 -0.12749735945220664 1106 18-19 -0.05353581459752377 1106 20-21 0.07220231826291723 1106 22-23 0.1520199914414846 1106 24-25 -0.07183164203328118 1106 26-27 -0.009027897138039975 1106 28-29 0.14503673308046672 1106 30-31 0.22541379604407297 1106 32-33 0.1984383438556776 1106 34-35 0.19113045483008761 1106 36-37 0.12170844171743767 1106 38-39 0.18500563015156501 1106 40-41 0.21119843993812282 1106 42-43 0.2638370012311029 1106 44-45 0.3439814686403153 1106 46-47 0.310133657126876 1106 48-49 0.41831380929270523 1106 50-51 0.34944886265965636 1106 52-53 0.1566459082001117 1106 54-55 0.2702182009892553 1106 56-57 0.2675213227608353 1106 58-59 0.4132934154354402 1106 60-61 0.42431040545020693 1106 62-63 0.6574497141862921 1106 64-65 0.572068646448848 1106 66-67 0.578863276478657 1106 68-69 0.569204677538842 1106 70-71 0.4676137982669957 1106 72-73 0.6367096912126833 1106 74-75 0.8103778197745477 1106 76-77 0.9433278273824293 1106 78-79 0.9787310499740194 1106 80-81 0.9807885531833129 1106 82-83 0.7005959271658639 1106 84-85 0.6583685692056029 1106 86-87 0.8402627008098449 1106 88-89 0.8496547167083008 1106 90-91 1.116532960283866 1106 92-93 1.04230162499778 1106 94-95 1.2731153628525362 1106 96-97 1.6708937561609218 1106 98-99 2.1295501965549875 1106 100-101 2.802015647871354 1107 1 0.13381956725416444 1107 2 0.1173191580679358 1107 3 0.19177659369071165 1107 4 0.23538646409529917 1107 5 0.14078096549804542 1107 6 0.04577019312016262 1107 7 0.10320436849875136 1107 8 0.19816097078525985 1107 9 0.21004150345034844 1107 10-11 0.2551640291721071 1107 12-13 0.16580564900831973 1107 14-15 0.455305777173443 1107 16-17 0.4530961343883817 1107 18-19 0.49764743434829484 1107 20-21 0.42643362439618926 1107 22-23 0.3412317262070843 1107 24-25 0.3952986430148897 1107 26-27 0.3772305786585832 1107 28-29 0.36192080872754673 1107 30-31 0.4457370142745134 1107 32-33 0.2218010703360349 1107 34-35 0.31023748220872704 1107 36-37 0.3770627362857013 1107 38-39 0.42971896717804725 1107 40-41 0.49620424941611674 1107 42-43 0.5753752932521721 1107 44-45 0.4180013994433125 1107 46-47 0.4626005385428513 1107 48-49 0.4007200958394108 1107 50-51 0.4658037020802084 1107 52-53 0.47430257982896507 1107 54-55 0.5559221736995212 1107 56-57 0.5989708502135613 1107 58-59 0.6661119051535422 1107 60-61 0.7715431713620617 1107 62-63 0.8026423996393461 1107 64-65 0.7774306540585414 1107 66-67 0.7599599797803762 1107 68-69 0.742216365646243 1107 70-71 0.967992611188123 1107 72-73 1.1888357929807576 1107 74-75 1.0036124945691576 1107 76-77 1.1070272528178364 1107 78-79 1.2346119590946323 1107 80-81 1.1069482640544486 1107 82-83 1.0807187355032468 1107 84-85 1.2508872609825907 1107 86-87 1.4474213531325368 1107 88-89 1.2878510276707722 1107 90-91 1.2720991165219715 1107 92-93 1.2829048228323536 1107 94-95 1.4758148513601057 1107 96-97 1.471065570807795 1107 98-99 1.8696965011722533 1107 100-101 2.1595322157123036 1108 1 0.1791608327187575 1108 2 0.07054966335137891 1108 3 0.2325524274278905 1108 4 0.1715439480945662 1108 5 0.28407848169374716 1108 6 0.2896464652412831 1108 7 0.24856958632599202 1108 8 0.2731753143512705 1108 9 0.30758332260444377 1108 10-11 0.32102745614246686 1108 12-13 0.392195582331631 1108 14-15 0.39551343858730803 1108 16-17 0.5037425696640767 1108 18-19 0.5199342722465019 1108 20-21 0.551151348587517 1108 22-23 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-0.9573344612217731 2316 86-87 -1.287658471146905 2316 88-89 -1.3903071208345317 2316 90-91 -1.286670839547888 2316 92-93 -1.3260276390412855 2316 94-95 -1.1987449543472408 2316 96-97 -1.011049861886228 2316 98-99 -0.9218049810235414 2316 100-101 -0.8744600697264637 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7086.0 3 1200.0 4 1162.0 5 1317.0 6 1633.0 7 1931.0 8 2377.0 9 2861.0 10 3633.0 11 4816.0 12 6528.0 13 8235.0 14 9178.0 15 11567.0 16 14600.0 17 19101.0 18 17013.0 19 12446.0 20 12547.0 21 13665.0 22 14975.0 23 16510.0 24 18714.0 25 21522.0 26 24924.0 27 29836.0 28 35668.0 29 42787.0 30 52545.0 31 66289.0 32 84756.0 33 111025.0 34 150512.0 35 212500.0 36 308136.0 37 408078.0 38 278221.0 39 27379.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84375912428328 18.74483008055935 14.874208578861348 23.537202216296023 2 15.340441666699157 20.069946156275464 39.66066951805773 24.92894265896765 3 18.67528356428464 23.68037105548874 30.428460322441687 27.215885057784934 4 13.037264919951705 16.34444008095943 36.48831165287621 34.12998334621265 5 14.150691512323274 36.47426646489234 34.38647709234488 14.988564930439502 6 32.72877457161462 35.3177647154512 17.109310049078697 14.84415066385549 7 28.499128742535383 32.48192956499464 20.912955096880516 18.10598659558946 8 26.33644578503968 34.15725389900694 20.21819145303974 19.28810886291364 9 26.059930963909235 16.646640323136054 20.692659837740347 36.60076887521437 10-11 24.759766071161554 26.192743374297343 27.43452228163906 21.61296827290204 12-13 25.51515110189832 23.666704981141486 27.994568436146004 22.82357548081419 14-15 22.915520108160443 24.995032256242066 26.000294168355865 26.089153467241626 16-17 22.351984866815116 28.172796502691362 26.855022997843403 22.62019563265012 18-19 22.280666508380058 26.79780103373331 29.045032540073905 21.876499917812723 20-21 22.629059717512035 25.92570227537025 29.25801619504175 22.18722181207596 22-23 22.847703080414096 25.569555224432953 29.147773200494736 22.434968494658214 24-25 22.66940687719476 25.91911769181494 28.960829289445165 22.450646141545135 26-27 22.30439251355623 26.073254905360226 29.023422956606183 22.59892962447736 28-29 22.31287782242775 26.200989172388212 29.14736788528542 22.338765119898614 30-31 22.368259882259114 26.003932813422704 28.974923748470406 22.652883555847776 32-33 22.259292929563518 26.09228771533853 28.93805979981331 22.710359555284644 34-35 22.835114671487574 25.874205569288332 28.79368288325232 22.49699687597177 36-37 22.711701974575966 25.698705570092855 28.7304370353579 22.85915541997327 38-39 22.37912857160249 26.155537429541887 28.781120268806077 22.68421373004955 40-41 22.668962439427364 26.051753736494337 28.76173623333712 22.517547590741174 42-43 22.47784085250473 26.04156698367987 28.421223594509453 23.059368569305942 44-45 22.58148245002749 26.12985085311318 28.464498541256084 22.824168155603246 46-47 22.730656111599576 26.417243470793185 27.975570305010823 22.87653011259642 48-49 22.684154428747906 26.593380797147592 27.768742114763988 22.953722659340514 50-51 22.76677778145779 26.63445409257661 27.75476686350415 22.84400126246145 52-53 22.888406484354775 26.603351680455628 27.47314118175147 23.035100653438132 54-55 22.80157974721025 26.50060279058804 27.608490739475883 23.089326722725826 56-57 22.81931052749328 27.014649629273848 27.47531555603811 22.69072428719476 58-59 22.878851764284295 26.43948187416499 27.695066469834416 22.986599891716295 60-61 22.743870046992615 26.367599004151966 28.0031620561058 22.88536889274962 62-63 22.515449348949428 26.59547504084968 28.21553537006733 22.673540240133562 64-65 22.642247139257186 26.656331320553896 27.792716258961768 22.908705281227153 66-67 22.528143928631685 26.713864763550582 27.61463603586627 23.14335527195146 68-69 22.423329321687792 26.569064296460198 27.94737253448753 23.060233847364476 70-71 22.57067189426386 26.563896858182922 27.80627895970037 23.05915228785285 72-73 22.59532290771175 26.415957029552406 27.800391539036674 23.188328523699163 74-75 22.530251691443336 26.897193308192673 27.37434479319824 23.198210207165744 76-77 22.73077804365448 26.583533593117085 27.558075743124306 23.127612620104127 78-79 22.657478411911516 26.60459018245743 27.574602214463876 23.16332919116718 80-81 22.590402883327922 26.742467167009053 27.39159092663336 23.275539023029662 82-83 22.686706642405596 26.605975031355072 27.549975152124755 23.15734317411458 84-85 22.544988229324748 26.662228975606737 27.44613720013021 23.346645594938305 86-87 22.611590039830066 26.6747484112652 27.607549573322355 23.106111975582387 88-89 22.63379124939523 26.44619265102306 27.47788559800864 23.442130501573068 90-91 22.498289476567653 26.336533615133444 27.68184507649266 23.48333183180624 92-93 22.503789174586906 26.367079407707884 27.68314343836702 23.445987979338195 94-95 22.473787678614126 26.217361514551364 27.68065452523205 23.62819628160246 96-97 22.456380734242018 26.166763319743474 27.638689861457433 23.73816608455708 98-99 22.239982236198664 26.175117092326722 27.61185217576132 23.97304849571329 100-101 22.440071711393774 25.729846508013487 28.169290111735002 23.66079166885774 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2518.0 1 1474.0 2 408.5 3 402.5 4 353.5 5 362.5 6 544.5 7 1023.0 8 1803.5 9 2335.0 10 2306.5 11 2233.0 12 2428.5 13 2585.0 14 2681.5 15 2762.0 16 2816.5 17 2896.0 18 3020.0 19 3202.5 20 3478.5 21 3811.5 22 4387.5 23 5202.0 24 6193.5 25 7795.5 26 9904.5 27 11846.0 28 13017.0 29 14774.5 30 17753.0 31 21182.0 32 25047.0 33 28281.0 34 31760.5 35 35610.0 36 41409.5 37 47689.5 38 51326.5 39 53591.5 40 55236.0 41 59750.5 42 64397.0 43 68098.0 44 73135.5 45 76993.5 46 80024.0 47 84192.0 48 89163.5 49 90992.5 50 88877.0 51 84370.0 52 79717.0 53 79208.0 54 78497.0 55 72074.5 56 63886.0 57 56077.0 58 49334.0 59 43411.5 60 36072.5 61 28717.0 62 22331.0 63 16759.0 64 12652.5 65 9517.0 66 6770.0 67 4979.0 68 3627.5 69 2388.0 70 1622.0 71 1079.5 72 705.5 73 489.0 74 354.5 75 265.0 76 186.0 77 103.5 78 66.0 79 46.5 80 20.0 81 13.5 82 16.0 83 12.0 84 10.0 85 8.0 86 9.0 87 9.5 88 9.0 89 10.0 90 8.0 91 5.0 92 3.5 93 3.5 94 3.5 95 2.0 96 4.0 97 3.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2791558149279095 2 0.26296934681525164 3 0.25188671999937784 4 0.23759596436838262 5 0.23409618747915933 6 0.1964249779076584 7 0.1905434084132692 8 0.20041083492038494 9 0.20148021119209206 10-11 0.20274401951320048 12-13 0.19880677051282428 14-15 0.19579307374710417 16-17 0.19627915387060743 18-19 0.1962062418520819 20-21 0.1850020950053323 22-23 0.1776136771280831 24-25 0.1779296292083602 26-27 0.18872060795013207 28-29 0.2014072991735666 30-31 0.1962062418520819 32-33 0.2012614751365156 34-35 0.19421331334571865 36-37 0.1820127022457874 38-39 0.18223143830136385 40-41 0.177151901010755 42-43 0.18906086403658431 44-45 0.18446740686947874 46-47 0.18420006280155193 48-49 0.19397027328396704 50-51 0.20133438715504107 52-53 0.2121010618906378 54-55 0.18993580825889017 56-57 0.16524293798492568 58-59 0.16575332211460408 60-61 0.17241261980659844 62-63 0.18026281380117573 64-65 0.1691801869853019 66-67 0.1744784603314872 68-69 0.1783671013195131 70-71 0.172947307942452 72-73 0.17418681225738525 74-75 0.17637417281314982 76-77 0.18954694416008758 78-79 0.19445635340747028 80-81 0.18536665509795974 82-83 0.1836653746656984 84-85 0.17758937312190792 86-87 0.17861014138126471 88-89 0.18492918298680683 90-91 0.18514791904238329 92-93 0.18113775802348156 94-95 0.18252308637546577 96-97 0.1719265396830952 98-99 0.17788102119600985 100-101 0.16944753105322868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2057274.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.239223966980155 #Duplication Level Percentage of deduplicated Percentage of total 1 65.47405968521873 25.69151292015724 2 15.09272858346331 11.8445391431872 3 6.869592314067073 8.086724141205702 4 3.675735626493869 5.769320539656043 5 2.156157886091775 4.230298110026283 6 1.4506522017872667 3.415347998647407 7 0.9643810224341239 2.648909405015853 8 0.6970599088296069 2.188167190477417 9 0.5450759920864727 1.9249523039254508 >10 2.7576432099680868 19.45619150231914 >50 0.20719974234604835 5.567965294871239 >100 0.10611131038125807 7.350578111612506 >500 0.0027329438038231515 0.6810138167623674 >1k 6.211235917779888E-4 0.4849134241752222 >5k 2.4844943671119556E-4 0.6595660979608898 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7482 0.3636851484051225 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6049 0.29402986670710857 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3563 0.17319034800420363 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2680 0.13026947309886772 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.860801235032378E-5 0.0 16-17 0.0 0.0 0.0 4.860801235032378E-5 0.0 18-19 0.0 0.0 0.0 7.291201852548567E-5 0.0 20-21 0.0 0.0 0.0 1.7012804322613323E-4 0.0 22-23 0.0 0.0 0.0 5.832961482038853E-4 0.0 24-25 0.0 0.0 0.0 7.534241914300186E-4 0.0 26-27 0.0 0.0 0.0 0.0011908963025829325 0.0 28-29 0.0 0.0 0.0 0.0024790086298665127 0.0 30-31 0.0 0.0 0.0 0.005371185364710778 0.0 32-33 0.0 0.0 0.0 0.010207682593567993 0.0 34-35 0.0 0.0 0.0 0.01584621202620555 0.0 36-37 0.0 0.0 0.0 0.020123717113034047 0.0 38-39 0.0 0.0 0.0 0.0254462944653945 0.0 40-41 0.0 0.0 0.0 0.03332079246614695 0.0 42-43 0.0 0.0 0.0 0.04236188276330717 0.0 44-45 0.0 0.0 0.0 0.05215639725189741 0.0 46-47 0.0 0.0 0.0 0.06401675226537643 0.0 48-49 0.0 0.0 0.0 0.0751236830874254 0.0 50-51 0.0 0.0 0.0 0.09109141514450676 0.0 52-53 0.0 0.0 0.0 0.13170340946320228 0.0 54-55 0.0 0.0 0.0 0.19005732828976596 0.0 56-57 0.0 0.0 0.0 0.23842230057833813 0.0 58-59 0.0 0.0 0.0 0.310459374881518 0.0 60-61 0.0 0.0 0.0 0.3947456682969794 0.0 62-63 0.0 0.0 0.0 0.44904081809229107 0.0 64-65 0.0 0.0 0.0 0.49818351857846843 0.0 66-67 0.0 0.0 0.0 0.6266058872080238 0.0 68-69 0.0 0.0 0.0 0.7561462401216368 0.0 70-71 0.0 0.0 0.0 0.8213052806772457 0.0 72-73 0.0 0.0 0.0 0.9523767859798938 0.0 74-75 0.0 0.0 0.0 1.1449860349180518 0.0 76-77 0.0 0.0 0.0 1.3405603726095796 0.0 78-79 0.0 0.0 0.0 1.5395129671594547 0.0 80-81 0.0 0.0 0.0 1.789965750794498 0.0 82-83 0.0 0.0 0.0 2.051015081121912 0.0 84-85 0.0 0.0 0.0 2.3450935558413706 2.430400617516189E-5 86-87 0.0 9.721602470064756E-5 0.0 2.7320619421622983 4.860801235032378E-5 88-89 0.0 9.721602470064756E-5 0.0 3.1693639252719863 4.860801235032378E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5500 0.0 37.60937 1 GTATCAA 8435 0.0 32.75371 1 ATCAACG 10170 0.0 26.965097 3 TCAACGC 10315 0.0 26.58151 4 TATCAAC 10460 0.0 26.312864 2 CAACGCA 10470 0.0 26.188627 5 AACGCAG 10675 0.0 25.762814 6 GTACATG 6325 0.0 24.88496 1 TACATGG 6360 0.0 23.917408 2 GAGTACT 6410 0.0 23.783842 12-13 ACATGGG 6560 0.0 22.242401 3 CGCAGAG 12105 0.0 22.209267 8 ACGCAGA 12290 0.0 22.029547 7 GTACTTT 7165 0.0 21.178488 14-15 GCAGAGT 12765 0.0 20.576729 9 AGTACTT 6780 0.0 20.34939 12-13 AGAGTAC 9875 0.0 20.200542 10-11 CATGGGG 4045 0.0 19.736212 4 CAGAGTA 12075 0.0 18.486794 10-11 GAGTACA 5230 0.0 18.09343 1 >>END_MODULE