FastQCFastQC Report
Thu 26 May 2016
SRR937765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057274
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88520.4302781253250661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76150.3701500140477156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47950.23307541921980252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69800.034.521281
GTATCAA102600.031.8695131
TACCGTA1056.905245E-527.1447057
ATCAACG124550.026.010143
TCAACGC125550.025.802974
TATCAAC127850.025.4130882
CAACGCA127700.025.405745
AACGCAG129350.025.0063936
GTACATG63850.024.0422971
GAGTACT80950.023.32617212-13
TACATGG64800.022.9441182
ACATGGG65100.022.181693
GTACTTT86550.021.81425914-15
ACGCAGA147600.021.724047
GGACCGA5250.021.7131256
CGCAGAG148200.021.4437668
AGTACTT85300.021.02282512-13
AGAGTAC119300.020.18787810-11
GCAGAGT154650.020.1500959
ACCGTAC1200.004762879619.7930158
Produced by FastQC (version 0.11.5)