##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937765_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2057274 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26041645400661 33.0 31.0 34.0 31.0 34.0 2 32.46740249475763 34.0 31.0 34.0 31.0 34.0 3 32.53723859826158 34.0 31.0 34.0 31.0 34.0 4 36.13877781958067 37.0 35.0 37.0 35.0 37.0 5 36.05294336097185 37.0 35.0 37.0 35.0 37.0 6 36.09479583176572 37.0 35.0 37.0 35.0 37.0 7 36.046520298219875 37.0 35.0 37.0 35.0 37.0 8 36.01269932930665 37.0 35.0 37.0 35.0 37.0 9 37.71690110311023 39.0 37.0 39.0 35.0 39.0 10-11 37.637522760701785 39.0 37.0 39.0 35.0 39.0 12-13 37.53746292423858 39.0 37.0 39.0 35.0 39.0 14-15 38.78522452526985 40.0 38.0 41.0 35.0 41.0 16-17 38.74872525487611 40.0 38.0 41.0 35.0 41.0 18-19 38.71361593059554 40.0 38.0 41.0 34.5 41.0 20-21 38.598343001466986 40.0 38.0 41.0 34.0 41.0 22-23 38.54172511780152 40.0 38.0 41.0 34.0 41.0 24-25 38.55046799794291 40.0 38.0 41.0 34.0 41.0 26-27 38.378898727150585 40.0 38.0 41.0 34.0 41.0 28-29 38.34510619392458 40.0 38.0 41.0 34.0 41.0 30-31 38.37223675601791 40.0 38.0 41.0 34.0 41.0 32-33 38.25470549863557 40.0 38.0 41.0 34.0 41.0 34-35 38.11531157249836 40.0 38.0 41.0 34.0 41.0 36-37 37.953488694262404 40.0 37.0 41.0 33.0 41.0 38-39 37.81945185716633 40.0 37.0 41.0 33.0 41.0 40-41 37.65144652583953 40.0 37.0 41.0 33.0 41.0 42-43 37.425768760019324 40.0 37.0 41.0 32.5 41.0 44-45 37.11954314301352 40.0 36.5 41.0 31.5 41.0 46-47 36.7859803798619 40.0 36.0 41.0 31.0 41.0 48-49 36.45109596485446 39.0 36.0 41.0 30.0 41.0 50-51 36.1929152363759 39.0 35.5 41.0 30.0 41.0 52-53 35.924160806970775 39.0 35.0 40.0 29.0 41.0 54-55 35.69010739454249 39.0 35.0 40.0 28.5 41.0 56-57 35.39635216310516 38.0 35.0 40.0 28.0 41.0 58-59 35.1284036545448 38.0 34.0 40.0 28.0 41.0 60-61 34.7716184134928 38.0 34.0 40.0 26.5 41.0 62-63 34.353860496948876 37.0 34.0 40.0 26.0 41.0 64-65 33.9401810356812 36.5 33.5 39.0 26.0 41.0 66-67 33.49705824309256 36.0 33.0 39.0 25.5 40.0 68-69 33.0193061303453 35.5 32.5 38.5 24.0 40.0 70-71 32.57923665005245 35.0 32.0 38.0 23.0 40.0 72-73 32.10322494718739 35.0 31.5 37.0 22.0 39.0 74-75 31.626274623603855 35.0 31.0 36.5 20.5 39.0 76-77 30.354038888354204 33.5 29.5 35.0 20.0 37.0 78-79 30.96878539270899 34.0 31.0 36.0 20.0 37.0 80-81 30.822165642495847 34.0 31.0 35.0 19.5 37.0 82-83 30.469276576673792 34.0 31.0 35.0 18.0 36.5 84-85 30.095827050747737 34.0 30.0 35.0 16.0 36.0 86-87 29.79983973938328 34.0 30.0 35.0 10.0 36.0 88-89 29.434790407111546 34.0 29.5 35.0 7.0 35.5 90-91 28.969888794589345 34.0 29.0 35.0 2.0 35.0 92-93 28.53497200664569 34.0 29.0 35.0 2.0 35.0 94-95 28.055036665023714 33.0 28.5 35.0 2.0 35.0 96-97 27.561394106959014 33.0 27.0 35.0 2.0 35.0 98-99 26.776090350629033 33.0 26.0 34.5 2.0 35.0 100-101 24.347513991816356 30.5 20.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2416738237221665 1101 2 0.23183354229984587 1101 3 0.2112069851993681 1101 4 0.16200412972685285 1101 5 0.13694155879797876 1101 6 0.10432442985099755 1101 7 0.13607621115254176 1101 8 0.08100043553253045 1101 9 0.2333315068985513 1101 10-11 0.14322745152404792 1101 12-13 0.26296150681538677 1101 14-15 0.283193667577315 1101 16-17 0.29114836959900714 1101 18-19 0.2720882594320386 1101 20-21 0.3366815755681003 1101 22-23 0.18921768739860312 1101 24-25 0.1044321692727479 1101 26-27 0.1486639341502709 1101 28-29 0.23140791418228446 1101 30-31 0.3302057721336027 1101 32-33 0.24549052045457387 1101 34-35 0.2731295259108535 1101 36-37 0.22814460634221945 1101 38-39 0.3108791751298199 1101 40-41 0.3195630962069558 1101 42-43 0.30843733516098126 1101 44-45 0.2308849206161696 1101 46-47 0.4525379728564687 1101 48-49 0.3732957399044423 1101 50-51 0.4720249372545169 1101 52-53 0.2458764810148253 1101 54-55 0.26699669420591476 1101 56-57 0.21333029450958207 1101 58-59 0.2239888311453626 1101 60-61 0.2710517129493084 1101 62-63 0.23321959473774 1101 64-65 0.3388310896021025 1101 66-67 0.20720255501585427 1101 68-69 0.31274621607046527 1101 70-71 0.2861554020431214 1101 72-73 0.26500367196240404 1101 74-75 0.2761794442493368 1101 76-77 0.25654448079667347 1101 78-79 0.3724644740463212 1101 80-81 0.3658635837147344 1101 82-83 0.448887420763203 1101 84-85 0.5144200847628149 1101 86-87 0.38394226096349726 1101 88-89 0.3648780002704193 1101 90-91 0.3999331184656931 1101 92-93 0.5627862343374197 1101 94-95 0.44829865477700537 1101 96-97 0.5528555948418656 1101 98-99 0.7786136633422025 1101 100-101 1.2262601803499145 1102 1 0.20179684319884217 1102 2 0.2091079360862622 1102 3 0.16574801707388076 1102 4 0.0971037410958644 1102 5 0.04997761856479599 1102 6 0.15174276939828957 1102 7 0.09860772749027547 1102 8 0.10186044376767 1102 9 0.10230744449197005 1102 10-11 0.18898853935240112 1102 12-13 0.20114506586303804 1102 14-15 0.2039766123736655 1102 16-17 0.14873400660976444 1102 18-19 0.18928384122222042 1102 20-21 0.216566255384663 1102 22-23 0.16284196752879154 1102 24-25 0.19333750180889098 1102 26-27 0.16494878543828406 1102 28-29 0.1857666871461987 1102 30-31 0.22692349009692947 1102 32-33 0.27139984082977975 1102 34-35 0.2815067376949969 1102 36-37 0.3418054228621443 1102 38-39 0.31885943617253076 1102 40-41 0.3531089591764598 1102 42-43 0.31164889933021556 1102 44-45 0.44698159046031805 1102 46-47 0.34592285573132386 1102 48-49 0.41101839895267034 1102 50-51 0.30267150867180703 1102 52-53 0.4090034939710705 1102 54-55 0.5068194553473333 1102 56-57 0.4500966054482163 1102 58-59 0.449042980020927 1102 60-61 0.4997482197827807 1102 62-63 0.4537331347768756 1102 64-65 0.5249971094905277 1102 66-67 0.47749851246386044 1102 68-69 0.5454358499451004 1102 70-71 0.5483018832122895 1102 72-73 0.6541624043146612 1102 74-75 0.64718666973992 1102 76-77 0.5136494055240952 1102 78-79 0.5502814846899575 1102 80-81 0.5874502303763975 1102 82-83 0.6268380409370415 1102 84-85 0.5842502772733518 1102 86-87 0.4614914884959447 1102 88-89 0.5746520133225559 1102 90-91 0.6697443447685067 1102 92-93 0.7901998781619071 1102 94-95 0.8849733779690823 1102 96-97 1.0068530227702794 1102 98-99 0.9634936474359712 1102 100-101 1.3475328622421898 1103 1 0.1376325772749496 1103 2 0.2202645002254897 1103 3 0.20758071732532812 1103 4 0.12823751273457162 1103 5 0.12767318497318314 1103 6 0.21008567076871287 1103 7 0.14937208362154308 1103 8 0.15215784863654136 1103 9 0.12921905290811964 1103 10-11 0.1604111609091703 1103 12-13 0.2506304490852429 1103 14-15 0.29030369329558425 1103 16-17 0.1855836615005657 1103 18-19 0.16863803559349577 1103 20-21 0.20678230670815623 1103 22-23 0.22340832157487966 1103 24-25 0.15619673017096147 1103 26-27 0.12148524916940318 1103 28-29 0.06936831367369223 1103 30-31 0.13882923348101173 1103 32-33 0.17910049984046594 1103 34-35 0.25301339495276665 1103 36-37 0.13124737534723607 1103 38-39 0.242763653328538 1103 40-41 0.29956754335538704 1103 42-43 0.23169174395228254 1103 44-45 0.25987484066623523 1103 46-47 0.3552415998459111 1103 48-49 0.2786304381439706 1103 50-51 0.04839170433331219 1103 52-53 0.009429650073307982 1103 54-55 0.16541704505592492 1103 56-57 0.3300957592688647 1103 58-59 0.22448823338176993 1103 60-61 0.31497632333960013 1103 62-63 0.07072625976378077 1103 64-65 0.16797162784757091 1103 66-67 0.2697189802224855 1103 68-69 0.3557486413044444 1103 70-71 0.2180816553338616 1103 72-73 0.15298784527890064 1103 74-75 0.2309212589897136 1103 76-77 0.13046611376223538 1103 78-79 0.2335672301390268 1103 80-81 0.25480829931750293 1103 82-83 0.3957642199404603 1103 84-85 0.25514240648571374 1103 86-87 0.3297014814858521 1103 88-89 0.37749445783376245 1103 90-91 0.552251275287869 1103 92-93 0.7741341152768833 1103 94-95 0.7181541661269151 1103 96-97 0.8042279066146953 1103 98-99 0.7240083961866901 1103 100-101 1.4747985268461967 1104 1 0.21370347440486626 1104 2 0.23153908387929079 1104 3 0.2516542099287591 1104 4 0.1732631635191595 1104 5 0.20494865140514662 1104 6 0.19056874072227714 1104 7 0.2202289070474066 1104 8 0.2209193954541746 1104 9 0.2436511543165949 1104 10-11 0.2068108664735746 1104 12-13 0.2914711631311988 1104 14-15 0.30130406067931403 1104 16-17 0.3040117216880631 1104 18-19 0.3079101664984023 1104 20-21 0.3698329528013389 1104 22-23 0.3101336771309633 1104 24-25 0.2509328350048321 1104 26-27 0.3781166575705939 1104 28-29 0.3715609205387409 1104 30-31 0.263263290745293 1104 32-33 0.19548739983544294 1104 34-35 0.2466046633781218 1104 36-37 0.2860460939973777 1104 38-39 0.36475586426495 1104 40-41 0.37152420529100283 1104 42-43 0.32003563626391696 1104 44-45 0.18500421788474597 1104 46-47 0.21152815650890489 1104 48-49 0.09732458722775306 1104 50-51 0.055275854236441546 1104 52-53 0.18706001182733445 1104 54-55 0.20231466129057907 1104 56-57 0.04657761077712763 1104 58-59 0.02169109527456925 1104 60-61 0.026392772615544402 1104 62-63 0.08363391693925593 1104 64-65 0.09177287325197625 1104 66-67 0.031099616192790336 1104 68-69 -0.058900718681393016 1104 70-71 0.14169194049235045 1104 72-73 -0.030545765045900453 1104 74-75 0.19318777870288883 1104 76-77 -0.01673053696685045 1104 78-79 -0.009399287724015437 1104 80-81 0.0018249584514791195 1104 82-83 -0.037425463713077534 1104 84-85 -0.0060647794342401085 1104 86-87 0.11197310228241619 1104 88-89 0.14740685399499753 1104 90-91 0.056411763828933204 1104 92-93 0.242635916514363 1104 94-95 0.2950034410065925 1104 96-97 0.5088652671608571 1104 98-99 0.6043106816306327 1104 100-101 0.8385556597730321 1105 1 0.17588984983901668 1105 2 0.13182705632755898 1105 3 0.15917597504522263 1105 4 0.16239418889333734 1105 5 0.10160562146688079 1105 6 0.1392477872785136 1105 7 0.12882273214907514 1105 8 0.0771881251571358 1105 9 0.32495217120624176 1105 10-11 0.26410390456200616 1105 12-13 0.12942344496803315 1105 14-15 0.11949898751591803 1105 16-17 0.34756912182766797 1105 18-19 0.4219173720789655 1105 20-21 0.3601535885976901 1105 22-23 0.2991414051579113 1105 24-25 0.2857894130227976 1105 26-27 0.2679598818589213 1105 28-29 0.19274476315281674 1105 30-31 0.21818086960335137 1105 32-33 0.18241579124391905 1105 34-35 0.29905044389212065 1105 36-37 0.3032183842769456 1105 38-39 0.33609654528426347 1105 40-41 0.3336128832508649 1105 42-43 0.3267448927600185 1105 44-45 0.49469058286997125 1105 46-47 0.38608062893418804 1105 48-49 0.4725288637649001 1105 50-51 0.38613174680475026 1105 52-53 0.4989275904764199 1105 54-55 0.5980772031385371 1105 56-57 0.5534488256530565 1105 58-59 0.5513953070283577 1105 60-61 0.469807126934505 1105 62-63 0.549660273314565 1105 64-65 0.4591634416318442 1105 66-67 0.41478789960114426 1105 68-69 0.4782128277264732 1105 70-71 0.28275612198604705 1105 72-73 0.4599174986139829 1105 74-75 0.39665818107019746 1105 76-77 0.35151893327251216 1105 78-79 0.5109426847827656 1105 80-81 0.6147663734034765 1105 82-83 0.709086161763917 1105 84-85 0.8119676665761482 1105 86-87 0.8825883142493645 1105 88-89 1.0271543243095955 1105 90-91 0.9575299035854314 1105 92-93 1.032955716396497 1105 94-95 1.3207997537889753 1105 96-97 1.2090432124001147 1105 98-99 1.302678872586874 1105 100-101 1.5007595894057708 1106 1 -0.3745969300023795 1106 2 -0.27859581499478026 1106 3 -0.30484765536046865 1106 4 -0.23841593438983466 1106 5 -0.2654665593230945 1106 6 -0.15451760275620785 1106 7 -0.2707466862399599 1106 8 -0.17332702528851485 1106 9 -0.5279455670185982 1106 10-11 -0.5120432044869929 1106 12-13 -0.7954290686132026 1106 14-15 -0.5495137969027866 1106 16-17 -0.3858622416717239 1106 18-19 -0.5055862319379898 1106 20-21 -0.48494969940602317 1106 22-23 -0.5921119646997397 1106 24-25 -0.5825397620034707 1106 26-27 -0.5839903596305689 1106 28-29 -0.5731902858102274 1106 30-31 -0.6545922415795573 1106 32-33 -0.8244128367319377 1106 34-35 -1.035961936007915 1106 36-37 -0.8758825933550725 1106 38-39 -0.8861915507359868 1106 40-41 -1.04526092593035 1106 42-43 -1.3082611750199362 1106 44-45 -1.2538647560976628 1106 46-47 -1.2014769606775602 1106 48-49 -1.1735607439063003 1106 50-51 -1.363386020795204 1106 52-53 -1.4584045139165482 1106 54-55 -1.440785094907561 1106 56-57 -1.5750547403554336 1106 58-59 -1.5711236225446754 1106 60-61 -1.6020797761629169 1106 62-63 -1.700059753617147 1106 64-65 -2.052718615386283 1106 66-67 -2.005082111448459 1106 68-69 -1.9496179205787527 1106 70-71 -1.8644431971194528 1106 72-73 -2.0207867575196303 1106 74-75 -2.0960845776384893 1106 76-77 -1.9612547616118192 1106 78-79 -1.990960113997705 1106 80-81 -1.934053463757401 1106 82-83 -1.9979951546321217 1106 84-85 -1.9550231353753134 1106 86-87 -1.7788713058288828 1106 88-89 -2.0394334521680513 1106 90-91 -2.388348485615822 1106 92-93 -2.3935522015812367 1106 94-95 -2.241956294308025 1106 96-97 -2.1547653308168684 1106 98-99 -2.20077684347903 1106 100-101 -1.3998357181777301 1107 1 0.14974588467261185 1107 2 0.21053487584295993 1107 3 0.12355214311258322 1107 4 0.10669441529590529 1107 5 0.1134835247604542 1107 6 0.12813344937103466 1107 7 0.1115929565892273 1107 8 0.14455704807884473 1107 9 0.154982318703766 1107 10-11 0.1161068890193846 1107 12-13 0.11976775072609058 1107 14-15 0.21555873988234708 1107 16-17 0.21052987871555473 1107 18-19 0.24159147881611887 1107 20-21 0.18717688870160742 1107 22-23 0.21091272539177197 1107 24-25 0.26969527222647827 1107 26-27 0.16649787969104324 1107 28-29 0.22955855856339014 1107 30-31 0.20067006642080543 1107 32-33 0.1514820090566218 1107 34-35 0.12126993321538038 1107 36-37 0.19106432648172955 1107 38-39 0.20498077836695927 1107 40-41 0.06320438400093309 1107 42-43 -0.020341507676192805 1107 44-45 -0.24443527799320464 1107 46-47 -0.17670574924371607 1107 48-49 -0.12667438094349137 1107 50-51 -0.3088759053123624 1107 52-53 -0.10227363386231758 1107 54-55 -0.32853754503305055 1107 56-57 -0.2960970192210155 1107 58-59 -0.15152277053856267 1107 60-61 -0.10029798760359654 1107 62-63 -0.09755951234712512 1107 64-65 -0.12269527868657093 1107 66-67 -0.014471504534682822 1107 68-69 0.11200206128990686 1107 70-71 -0.06801277749200096 1107 72-73 0.08916435775576304 1107 74-75 0.21643551236472547 1107 76-77 -0.05468534005953174 1107 78-79 0.003448120946853095 1107 80-81 0.018936118697293836 1107 82-83 0.11782528120411229 1107 84-85 0.1962736814562902 1107 86-87 0.37731892284950064 1107 88-89 0.44352547569639356 1107 90-91 0.4149121551517432 1107 92-93 0.3832063528656171 1107 94-95 0.5941126990374848 1107 96-97 0.6384045240335681 1107 98-99 0.9708020426969917 1107 100-101 1.6925944446016423 1108 1 0.1330349339866288 1108 2 0.11018450291024351 1108 3 0.03529158596948179 1108 4 0.05643121782259897 1108 5 -9.98742135713826E-4 1108 6 0.06117824028918761 1108 7 -0.0041749394463792555 1108 8 0.04346805112580654 1108 9 -0.02348395024227301 1108 10-11 0.04836793635036685 1108 12-13 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40-41 -0.5489335494966952 2316 42-43 -0.41892717144990144 2316 44-45 -0.5770453712108221 2316 46-47 -0.6144624548403428 2316 48-49 -0.6466572374740807 2316 50-51 -0.6007524009014844 2316 52-53 -0.5889160139473191 2316 54-55 -0.5745999044030299 2316 56-57 -0.6444755327393565 2316 58-59 -0.5520013590595809 2316 60-61 -0.4697576111615831 2316 62-63 -0.48566265888807436 2316 64-65 -0.4714130900369682 2316 66-67 -0.46096959375215363 2316 68-69 -0.40252524325669015 2316 70-71 -0.30992320286433994 2316 72-73 -0.38732587403822905 2316 74-75 -0.42130154967320266 2316 76-77 -0.25319242959844246 2316 78-79 -0.2768297461198266 2316 80-81 -0.22765304520141427 2316 82-83 -0.16675631099560917 2316 84-85 -0.33919187088486424 2316 86-87 -0.17405791203745835 2316 88-89 0.022349719419384684 2316 90-91 -0.12102662837623868 2316 92-93 0.047372930243380296 2316 94-95 -0.011319637831689988 2316 96-97 -0.002413005163901971 2316 98-99 -0.2472950321985401 2316 100-101 0.34043901047899183 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 15.0 5 40.0 6 88.0 7 215.0 8 517.0 9 997.0 10 1728.0 11 2946.0 12 4312.0 13 6029.0 14 7696.0 15 10578.0 16 14590.0 17 20031.0 18 18610.0 19 11564.0 20 9984.0 21 10101.0 22 10608.0 23 11462.0 24 12956.0 25 15514.0 26 18621.0 27 23166.0 28 29216.0 29 36195.0 30 46388.0 31 58058.0 32 75700.0 33 100814.0 34 140018.0 35 203818.0 36 322768.0 37 483606.0 38 327654.0 39 20661.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.29339386521445 19.534952930724355 15.28415409759696 22.88749910646424 2 15.886410852419269 20.92127737967816 38.62543346195013 24.56687830595244 3 19.132745565247994 24.10447028446381 30.394687338682157 26.36809681160604 4 13.728124561008675 16.995799779611165 35.839531262987464 33.4365443963927 5 14.141330666820927 37.16809771335542 33.91140716701367 14.779164452809972 6 32.855565179942005 35.86338037616768 16.5888452388938 14.692209204996514 7 28.640569996996025 32.838114903508234 20.478750035240807 18.04256506425493 8 26.725657350455016 34.57730958540282 19.62796399507309 19.06906906906907 9 26.268596210324922 16.675221676840323 20.384887963392334 36.67129414944242 10-11 25.061513439629334 26.590454164102596 26.857895448054077 21.490136948213994 12-13 25.83424437853916 23.996882110831383 27.682568355126314 22.486305155503143 14-15 23.027802810904138 25.34817919246537 25.89990443664772 25.724113559982776 16-17 22.338346763727145 28.62474808897599 26.636242911736602 22.40066223556026 18-19 22.269517818239088 26.992928506363274 29.139847195852376 21.597706479545263 20-21 22.688590569772767 25.9733602946627 29.545623016938734 21.792426118625798 22-23 22.936454987683756 25.69509914363523 29.266092840294718 22.102353028386297 24-25 22.775891786898587 26.048158874316208 29.18775039202362 21.98819894676159 26-27 22.431422491891492 26.203467974883978 29.248329993522916 22.116779539701618 28-29 22.40626586620968 26.365645452903802 29.369522246172846 21.85856643471367 30-31 22.448260391304412 26.18169781064545 29.149023974941585 22.221017823108554 32-33 22.464033698979183 26.214581146984383 29.182126047442413 22.139259106594018 34-35 22.98728803261014 26.0056997755282 29.002213608882432 22.004798582979223 36-37 22.85534226785783 25.862493030788965 28.87229294455789 22.409871756795315 38-39 22.54410995912794 26.20453721879954 29.00180748937854 22.249545332693977 40-41 22.908752154857616 26.236097781016156 28.860108575901904 21.995041488224324 42-43 22.655397281559438 26.24074977112961 28.479352560552805 22.62450038675814 44-45 22.786536941603305 26.399060115473194 28.424361558061783 22.390041384861714 46-47 22.926236369098138 26.712751923176008 27.880364987843137 22.480646719882717 48-49 23.024303034014913 26.879890573642594 27.517530479654145 22.578275912688344 50-51 23.02821597900912 26.98865100127645 27.528175634358863 22.454957385355574 52-53 23.241823889282614 27.045060599609 27.13430491028419 22.578810600824198 54-55 23.13549386226628 26.941890093395436 27.332965856759966 22.589650187578318 56-57 23.061925635574067 27.3911496475433 27.21572333097098 22.331201385911648 58-59 23.12951507674719 26.794121735850453 27.44814254202406 22.628220645378303 60-61 23.041388750356052 26.8127629085868 27.70659134369073 22.43925699736642 62-63 22.703417240484253 27.039373462164008 27.913224004191957 22.343985293159783 64-65 23.01905336868108 27.0232356020637 27.547108455169315 22.410602574085903 66-67 22.853834734702332 27.161501093194197 27.288586741484117 22.696077430619354 68-69 22.73168279966597 26.960069490014455 27.597709396026005 22.710538314293576 70-71 22.892332280483785 26.959802145946533 27.467391314914785 22.6804742586549 72-73 22.934232387129764 26.73294855230757 27.43893132368367 22.893887736878995 74-75 22.861879360746308 27.404808499013743 26.847104469312306 22.886207670927643 76-77 23.123268947160174 26.978929398806383 27.190714508616743 22.7070871454167 78-79 22.953797112100773 26.976304566139465 27.13775607916106 22.9321422425987 80-81 22.821972182606693 27.28943738170025 26.972586053194664 22.916004382498393 82-83 22.94370695144536 27.246788219339223 27.035756123406262 22.773748705809155 84-85 22.805813428125553 27.19672634360608 27.00416546868024 22.993294759588125 86-87 22.778030539442 27.051452553233062 27.16602163834278 23.004495268982158 88-89 22.921059615782827 27.02500979451449 26.99771639557978 23.056214194122905 90-91 22.806659765162507 26.92242905398754 27.053807182603933 23.21710399824602 92-93 22.659476060180214 26.965041675153035 27.141104431658047 23.234377833008704 94-95 22.68142211489573 26.867884394592068 27.13622492677203 23.314468563740174 96-97 22.653302379751068 26.7381982176414 27.11794831412831 23.49055108847922 98-99 22.366456573459427 26.856770517505918 26.85261450719804 23.924158401836618 100-101 22.209574285521217 26.904154567671483 27.236582208266547 23.64968893854075 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 317.0 1 252.0 2 236.5 3 328.5 4 319.0 5 313.5 6 546.0 7 1157.0 8 1931.5 9 2473.5 10 2585.0 11 2501.5 12 2651.5 13 2967.0 14 3224.0 15 3356.5 16 3444.0 17 3491.5 18 3544.0 19 3716.5 20 4006.5 21 4364.0 22 4944.5 23 5724.5 24 6685.5 25 8178.0 26 10164.0 27 12003.0 28 13164.0 29 14897.5 30 17854.0 31 21064.5 32 24871.0 33 28044.5 34 31663.5 35 35654.5 36 41000.5 37 47373.0 38 50809.5 39 53109.5 40 55150.5 41 59406.5 42 63960.0 43 67475.0 44 72623.0 45 76232.5 46 78633.5 47 83613.5 48 89785.5 49 90808.5 50 88459.5 51 84906.5 52 80309.0 53 79553.0 54 78689.5 55 72306.5 56 63836.0 57 55808.0 58 49248.0 59 43679.5 60 36379.0 61 28793.0 62 22414.0 63 16956.0 64 12562.0 65 9156.0 66 6603.5 67 4942.5 68 3506.5 69 2314.5 70 1569.0 71 955.5 72 613.5 73 437.5 74 301.0 75 206.0 76 129.5 77 79.0 78 39.5 79 19.5 80 8.0 81 10.0 82 7.5 83 2.0 84 1.0 85 2.0 86 2.5 87 2.0 88 2.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040684906337221 2 0.0 3 0.0 4 4.8608012350323786E-5 5 0.007534241914300186 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0054927053955865866 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.1595208027710456E-4 22-23 0.001531152389035199 24-25 0.0 26-27 0.0010450722655319612 28-29 0.0015797604013855226 30-31 4.8608012350323786E-5 32-33 4.8608012350323786E-5 34-35 0.0 36-37 5.346881358535615E-4 38-39 2.4304006175161893E-5 40-41 1.7012804322613323E-4 42-43 0.021047269347690196 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.9443204940129515E-4 84-85 1.2152003087580944E-4 86-87 0.0 88-89 0.0 90-91 0.011398578896150926 92-93 0.0025762246545671602 94-95 0.0 96-97 0.0 98-99 6.076001543790472E-4 100-101 0.013853283519842276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2057274.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.53313572729658 #Duplication Level Percentage of deduplicated Percentage of total 1 63.927984539922264 23.9941772050942 2 15.760606811726142 11.830899892181444 3 6.918277577671722 7.789939539655961 4 3.8589911921960116 5.793601607485398 5 2.295940050352877 4.308691476581533 6 1.5047659905459354 3.388715169658828 7 1.048594948831086 2.754993957620434 8 0.7791825360388017 2.339613110518681 9 0.578770789707549 1.955077434457926 >10 2.9884911713750184 20.204433482610327 >50 0.21983693058575948 5.712276407084942 >100 0.1133755576563682 7.621427605331128 >500 0.004404617881888982 1.0401490093802872 >1k 5.181903390457626E-4 0.46532470174688423 >5k 2.590951695228813E-4 0.8006794005920526 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8852 0.4302781253250661 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7615 0.3701500140477156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4795 0.23307541921980252 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.860801235032378E-5 0.0 16-17 0.0 0.0 0.0 4.860801235032378E-5 0.0 18-19 0.0 0.0 0.0 7.291201852548567E-5 0.0 20-21 0.0 0.0 0.0 1.7012804322613323E-4 0.0 22-23 0.0 0.0 0.0 6.319041605542091E-4 0.0 24-25 0.0 0.0 0.0 7.534241914300186E-4 0.0 26-27 0.0 0.0 0.0 0.0011908963025829325 0.0 28-29 0.0 0.0 0.0 0.0026005286607423224 0.0 30-31 0.0 0.0 0.0 0.005371185364710778 0.0 32-33 2.430400617516189E-5 0.0 0.0 0.010329202624443803 0.0 34-35 4.860801235032378E-5 0.0 0.0 0.016064948081782007 0.0 36-37 4.860801235032378E-5 0.0 0.0 0.020463973199486313 0.0 38-39 4.860801235032378E-5 0.0 0.0 0.02573794253949644 0.0 40-41 4.860801235032378E-5 0.0 0.0 0.033758264577299864 0.0 42-43 4.860801235032378E-5 0.0 0.0 0.04306669894238686 0.0 44-45 4.860801235032378E-5 0.0 0.0 0.05269108538775097 0.0 46-47 4.860801235032378E-5 0.0 0.0 0.06445422437652933 0.0 48-49 4.860801235032378E-5 0.0 0.0 0.07560976321092863 0.0 50-51 4.860801235032378E-5 0.0 0.0 0.09152888725565966 0.0 52-53 4.860801235032378E-5 0.0 0.0 0.13231100961758133 0.0 54-55 4.860801235032378E-5 0.0 0.0 0.19112670456147307 0.0 56-57 4.860801235032378E-5 0.0 0.0 0.2399534529673733 0.0 58-59 4.860801235032378E-5 0.0 0.0 0.31276825546815834 0.0 60-61 4.860801235032378E-5 0.0 0.0 0.39729758894537137 0.0 62-63 4.860801235032378E-5 0.0 0.0 0.4517628667839092 0.0 64-65 4.860801235032378E-5 0.0 0.0 0.5012215193503636 0.0 66-67 4.860801235032378E-5 0.0 0.0 0.6305431362084 0.0 68-69 4.860801235032378E-5 0.0 0.0 0.7611771693998952 0.0 70-71 4.860801235032378E-5 0.0 0.0 0.8267250740543068 0.0 72-73 4.860801235032378E-5 0.0 0.0 0.9598867238880189 0.0 74-75 4.860801235032378E-5 0.0 0.0 1.153419525060833 0.0 76-77 4.860801235032378E-5 2.430400617516189E-5 0.0 1.3502090630611188 0.0 78-79 4.860801235032378E-5 4.860801235032378E-5 0.0 1.5508629380432553 0.0 80-81 4.860801235032378E-5 4.860801235032378E-5 0.0 1.8039891623575666 0.0 82-83 4.860801235032378E-5 4.860801235032378E-5 0.0 2.0685139655680285 0.0 84-85 4.860801235032378E-5 4.860801235032378E-5 0.0 2.365849177114959 0.0 86-87 4.860801235032378E-5 4.860801235032378E-5 0.0 2.7580672287697214 0.0 88-89 4.860801235032378E-5 4.860801235032378E-5 0.0 3.2047262542568467 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6980 0.0 34.52128 1 GTATCAA 10260 0.0 31.869513 1 TACCGTA 105 6.905245E-5 27.144705 7 ATCAACG 12455 0.0 26.01014 3 TCAACGC 12555 0.0 25.80297 4 TATCAAC 12785 0.0 25.413088 2 CAACGCA 12770 0.0 25.40574 5 AACGCAG 12935 0.0 25.006393 6 GTACATG 6385 0.0 24.042297 1 GAGTACT 8095 0.0 23.326172 12-13 TACATGG 6480 0.0 22.944118 2 ACATGGG 6510 0.0 22.18169 3 GTACTTT 8655 0.0 21.814259 14-15 ACGCAGA 14760 0.0 21.72404 7 GGACCGA 525 0.0 21.713125 6 CGCAGAG 14820 0.0 21.443766 8 AGTACTT 8530 0.0 21.022825 12-13 AGAGTAC 11930 0.0 20.187878 10-11 GCAGAGT 15465 0.0 20.150095 9 ACCGTAC 120 0.0047628796 19.793015 8 >>END_MODULE