FastQCFastQC Report
Thu 26 May 2016
SRR937764_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937764_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873907
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70080.37397800424460764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55720.2973466666168599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35190.18778946874097807No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24330.12983568554896266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53850.035.317631
GTATCAA79100.032.8196141
ATCAACG97650.026.3303363
TCAACGC98700.026.0048584
TATCAAC99650.025.897962
CAACGCA100800.025.509565
AACGCAG102250.025.1330186
GTACATG57300.024.8104081
GTATAGG4350.024.0464361
TACATGG59850.023.3467372
ACATGGG60700.022.2362233
GGACCGT1954.2227657E-721.9239436
GAGTACT60550.021.84704612-13
ACGCAGA118650.021.4600647
CGCAGAG117850.021.4041948
GTACACG2457.4414856E-921.3473471
AGTACTT63650.020.74569912-13
GCAGAGT121000.020.1795629
CATGGGG37200.020.060174
GTACTTT65300.019.96584314-15
Produced by FastQC (version 0.11.5)