##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937764_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1873907 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84170559157952 33.0 31.0 34.0 30.0 34.0 2 31.957882648391834 33.0 31.0 34.0 30.0 34.0 3 31.99979508054562 34.0 31.0 34.0 30.0 34.0 4 35.617550390707756 37.0 35.0 37.0 33.0 37.0 5 35.609273032226255 37.0 35.0 37.0 33.0 37.0 6 35.66804008950284 37.0 35.0 37.0 33.0 37.0 7 35.59456685950797 37.0 35.0 37.0 33.0 37.0 8 35.5984731366071 37.0 35.0 37.0 33.0 37.0 9 37.17959642607664 39.0 37.0 39.0 34.0 39.0 10-11 37.11138146129984 39.0 37.0 39.0 33.0 39.0 12-13 37.01051412903628 39.0 37.0 39.0 33.0 39.0 14-15 38.177231580862866 40.0 38.0 41.0 33.0 41.0 16-17 38.07711001666571 40.0 38.0 41.0 33.0 41.0 18-19 37.991598035548186 40.0 38.0 41.0 33.0 41.0 20-21 37.999219811869 40.0 38.0 41.0 33.0 41.0 22-23 37.99940765470218 40.0 38.0 41.0 33.0 41.0 24-25 37.922150885823044 40.0 38.0 41.0 33.0 41.0 26-27 37.82518129234802 40.0 38.0 41.0 33.0 41.0 28-29 37.68262672587274 40.0 37.5 41.0 33.0 41.0 30-31 37.456428467367914 40.0 37.0 41.0 32.0 41.0 32-33 37.33407661105914 40.0 37.0 41.0 32.0 41.0 34-35 37.33400963868538 40.0 37.0 41.0 32.0 41.0 36-37 37.18731052288081 40.0 37.0 41.0 31.0 41.0 38-39 37.033369051932674 40.0 36.0 41.0 31.0 41.0 40-41 36.81809262679525 39.5 36.0 41.0 30.5 41.0 42-43 36.561147111356114 39.0 36.0 41.0 30.0 41.0 44-45 36.31790344985103 39.0 35.5 41.0 30.0 41.0 46-47 35.955720054410385 39.0 35.0 40.5 29.0 41.0 48-49 35.60711097188921 39.0 35.0 40.0 27.5 41.0 50-51 34.718905207142086 38.0 34.0 39.5 26.5 40.5 52-53 34.826267792371766 38.0 34.5 39.5 26.5 40.5 54-55 35.09605092461899 38.0 35.0 40.0 26.5 41.0 56-57 34.84459154056205 38.0 34.0 40.0 26.0 41.0 58-59 34.55339245757661 38.0 34.0 40.0 26.0 41.0 60-61 34.1903749759193 38.0 34.0 40.0 24.5 41.0 62-63 33.80581506979802 37.0 33.0 40.0 23.5 41.0 64-65 33.34849141392823 36.5 33.0 39.0 22.0 41.0 66-67 32.82192872965414 36.0 32.0 39.0 20.5 40.0 68-69 32.33907846013703 35.0 31.5 38.5 19.5 40.0 70-71 31.85305033814378 35.0 31.0 38.0 18.0 40.0 72-73 31.364010860731085 35.0 31.0 37.0 15.5 39.0 74-75 30.81252511464016 34.5 30.0 36.5 10.5 39.0 76-77 30.268219820940956 34.0 30.0 36.0 7.5 38.5 78-79 29.75081180656244 34.0 29.0 35.5 4.5 37.5 80-81 29.220171011688414 34.0 29.0 35.0 2.0 37.0 82-83 28.688791119303147 34.0 28.5 35.0 2.0 36.5 84-85 28.16195787731195 33.5 27.0 35.0 2.0 36.0 86-87 27.602130468587823 33.0 26.0 35.0 2.0 35.5 88-89 27.065074734231743 33.0 25.5 35.0 2.0 35.0 90-91 26.50692910587345 32.0 25.0 34.5 2.0 35.0 92-93 25.832124272976195 32.0 23.5 34.0 2.0 35.0 94-95 25.053941577677016 31.5 19.5 34.0 2.0 35.0 96-97 24.1415179622041 31.0 11.0 34.0 2.0 35.0 98-99 22.93548559240133 31.0 2.0 34.0 2.0 35.0 100-101 19.64966137593808 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14126836559123745 1101 2 0.124254411653137 1101 3 0.18638414908708612 1101 4 0.24244379882722455 1101 5 0.12595637615115152 1101 6 0.17784574898225003 1101 7 0.16516770666614633 1101 8 0.18013207278973198 1101 9 0.3198420189563933 1101 10-11 0.33873160064067775 1101 12-13 0.34587645484420193 1101 14-15 0.4981953384107527 1101 16-17 0.5455625891766474 1101 18-19 0.538699055477764 1101 20-21 0.528805906798425 1101 22-23 0.3959146148007804 1101 24-25 0.38273498454926624 1101 26-27 0.4816930288332344 1101 28-29 0.44110386067762875 1101 30-31 0.4111690429436763 1101 32-33 0.46449511722283887 1101 34-35 0.5653107682885548 1101 36-37 0.5145708078231976 1101 38-39 0.41091565536871855 1101 40-41 0.5403183411159986 1101 42-43 0.46845872274688105 1101 44-45 0.5530463855459615 1101 46-47 0.5363739493465758 1101 48-49 0.6065468813116226 1101 50-51 0.4214695466629905 1101 52-53 0.6417110457238877 1101 54-55 0.6047488357724546 1101 56-57 0.7184333132099923 1101 58-59 0.8179011918657935 1101 60-61 0.8679402643081886 1101 62-63 0.8143652603521403 1101 64-65 0.7368706222663235 1101 66-67 0.8562838367498955 1101 68-69 0.9588660462418446 1101 70-71 0.8827550189327091 1101 72-73 0.8364832258297561 1101 74-75 0.7914621122834014 1101 76-77 0.7965969070856573 1101 78-79 0.9191476600064377 1101 80-81 1.1811713090530418 1101 82-83 1.262446616940256 1101 84-85 1.467905900267862 1101 86-87 1.6900490320320536 1101 88-89 1.7498133578354498 1101 90-91 1.7267194176946212 1101 92-93 1.6666362175526892 1101 94-95 1.940645753286379 1101 96-97 2.3322520537558056 1101 98-99 2.778142075293662 1101 100-101 3.387783328849533 1102 1 0.056121768382929815 1102 2 0.0644334004997269 1102 3 -0.03387899123215732 1102 4 -0.048292860492885836 1102 5 -0.028254536825542687 1102 6 -0.034149175214636784 1102 7 0.02251760314647555 1102 8 -0.02162442223464467 1102 9 0.20891568488840306 1102 10-11 0.14327978737680525 1102 12-13 0.12293849985856298 1102 14-15 0.2390021239580733 1102 16-17 0.3637987475951334 1102 18-19 0.35252727961453445 1102 20-21 0.3433888227137416 1102 22-23 0.28746607409417635 1102 24-25 0.28684237785721933 1102 26-27 0.1979890282989416 1102 28-29 0.16438910745515756 1102 30-31 0.39863982368233764 1102 32-33 0.38304851333584367 1102 34-35 0.5538534785002653 1102 36-37 0.41319165868809193 1102 38-39 0.5047345293418743 1102 40-41 0.5882412690529506 1102 42-43 0.4936407170032098 1102 44-45 0.6421400875428844 1102 46-47 0.5934566982946805 1102 48-49 0.6564400222987317 1102 50-51 0.5738404810114872 1102 52-53 0.5556227534644833 1102 54-55 0.7339747757978401 1102 56-57 0.731827422606905 1102 58-59 0.7484595302081445 1102 60-61 0.7897261455611115 1102 62-63 0.7521532787853218 1102 64-65 0.8888608713813966 1102 66-67 0.9262364180629277 1102 68-69 0.7451580384945728 1102 70-71 0.9175276095959042 1102 72-73 0.8854981193046818 1102 74-75 0.9423336811072822 1102 76-77 0.9158014753084593 1102 78-79 0.9795697531073522 1102 80-81 1.1164715160923784 1102 82-83 1.3361592997693457 1102 84-85 1.3951616272430805 1102 86-87 1.5924166270363962 1102 88-89 1.6795702535811259 1102 90-91 1.8754237521633321 1102 92-93 1.9737323238774067 1102 94-95 1.9475915891242224 1102 96-97 1.8574340480121307 1102 98-99 2.2569339005791775 1102 100-101 2.7290208897925616 1103 1 0.08408284773233632 1103 2 -0.011328844719017184 1103 3 0.10243864229184041 1103 4 0.026700588209862985 1103 5 -0.009997700291229705 1103 6 0.003424833042615205 1103 7 -0.048205255815865655 1103 8 -0.046078952426967135 1103 9 -0.025568203676286316 1103 10-11 0.0076422522245067626 1103 12-13 0.10920277181801907 1103 14-15 0.08231875445586923 1103 16-17 0.12659755689983143 1103 18-19 0.18836972412587727 1103 20-21 0.1442711195628874 1103 22-23 -0.05674300716902536 1103 24-25 -0.04534584578765788 1103 26-27 -0.07671019462922146 1103 28-29 -0.07000667745683131 1103 30-31 -0.059012757338543054 1103 32-33 0.046761151323288175 1103 34-35 0.01512770224882587 1103 36-37 0.12092889531048456 1103 38-39 0.22772772788255935 1103 40-41 0.1744855898748483 1103 42-43 0.053416132350697865 1103 44-45 0.20512480625562546 1103 46-47 -6.522705858245104E-4 1103 48-49 -0.002817614659058165 1103 50-51 0.15282962654634957 1103 52-53 0.1791106641214384 1103 54-55 0.10894950990553554 1103 56-57 0.18521702337965706 1103 58-59 0.11011420479058387 1103 60-61 0.3203948962774703 1103 62-63 0.3624886576452937 1103 64-65 0.25462990331605795 1103 66-67 0.48628791530187243 1103 68-69 0.41623369254232045 1103 70-71 0.4753407271240384 1103 72-73 0.3902511984143864 1103 74-75 0.33287113479044805 1103 76-77 0.37746568553590265 1103 78-79 0.6882062176991965 1103 80-81 0.8341978758967556 1103 82-83 0.7739645414729779 1103 84-85 1.0531305253457965 1103 86-87 1.0613123517858405 1103 88-89 1.1736475717297203 1103 90-91 1.1645008729407174 1103 92-93 1.3104393969480306 1103 94-95 1.6928256689963064 1103 96-97 1.9585247260511842 1103 98-99 2.412797109492555 1103 100-101 2.9528008384950724 1104 1 0.20746535532668275 1104 2 0.16908071966296845 1104 3 0.15780539849788866 1104 4 0.20044470492692312 1104 5 0.15546410774428665 1104 6 0.22677238694050317 1104 7 0.2992764111723929 1104 8 0.3151720464304688 1104 9 0.45495521277477735 1104 10-11 0.3226243056228455 1104 12-13 0.31956341112718434 1104 14-15 0.42126404181868793 1104 16-17 0.5319692631958404 1104 18-19 0.40357968370688013 1104 20-21 0.555590763035184 1104 22-23 0.4527659641113715 1104 24-25 0.3661922582874766 1104 26-27 0.4563945422027871 1104 28-29 0.47889920112857 1104 30-31 0.5295052059317911 1104 32-33 0.40003619145436886 1104 34-35 0.5701860880547969 1104 36-37 0.49466018220254426 1104 38-39 0.5374149529126484 1104 40-41 0.504482326856369 1104 42-43 0.6503280338823032 1104 44-45 0.6410135646013231 1104 46-47 0.664687047978731 1104 48-49 0.8017937477168218 1104 50-51 0.6069973497373198 1104 52-53 0.559868048089811 1104 54-55 0.6954626867419407 1104 56-57 0.7714316794847846 1104 58-59 0.7829511417557384 1104 60-61 0.7085511494028438 1104 62-63 0.7434480861018429 1104 64-65 0.7964649824821421 1104 66-67 1.0093343724012627 1104 68-69 1.014269466539396 1104 70-71 1.246219751976522 1104 72-73 1.1419976204590618 1104 74-75 1.2038644663121119 1104 76-77 1.3975620405523124 1104 78-79 1.2879512880662425 1104 80-81 1.3168700354795746 1104 82-83 1.3869099359193804 1104 84-85 1.5686907290470522 1104 86-87 1.6622217035159998 1104 88-89 1.636557759924056 1104 90-91 1.7964611792805698 1104 92-93 1.9368424696406095 1104 94-95 2.2428162739276054 1104 96-97 2.397334037473467 1104 98-99 2.744437225829774 1104 100-101 3.234509700469392 1105 1 0.2076957296062929 1105 2 0.1020239164123602 1105 3 0.08155801663381723 1105 4 0.07308241996098275 1105 5 0.11909424265146384 1105 6 0.14536733149890324 1105 7 0.19757619478318844 1105 8 0.12915104930554122 1105 9 0.2535696063429498 1105 10-11 0.24182283079381506 1105 12-13 0.2855664011143091 1105 14-15 0.2965291077545231 1105 16-17 0.3127659679221395 1105 18-19 0.43151242959483227 1105 20-21 0.3865461820887077 1105 22-23 0.29261057801490864 1105 24-25 0.2902020825308682 1105 26-27 0.3219982529090686 1105 28-29 0.3425374126869656 1105 30-31 0.19676560583986458 1105 32-33 0.4060286330097753 1105 34-35 0.4566085868947951 1105 36-37 0.4931188427576956 1105 38-39 0.4768731342892991 1105 40-41 0.5584443850256022 1105 42-43 0.6311863705041176 1105 44-45 0.7647600574917064 1105 46-47 0.8032037845346309 1105 48-49 0.770616518886321 1105 50-51 0.5846781474719549 1105 52-53 0.600652697465577 1105 54-55 0.6602501136184316 1105 56-57 0.6522127187600759 1105 58-59 0.7814599425952693 1105 60-61 0.8546919194708877 1105 62-63 0.9615401642621535 1105 64-65 0.890646053413569 1105 66-67 1.0438447105148114 1105 68-69 0.7787871115830427 1105 70-71 0.8741791128694807 1105 72-73 1.012791257142009 1105 74-75 1.0951391895377434 1105 76-77 1.1038630210252478 1105 78-79 1.1467692566095842 1105 80-81 1.2261462049136256 1105 82-83 1.4455523863645183 1105 84-85 1.5756886865757913 1105 86-87 1.6367761867620985 1105 88-89 1.569567900728817 1105 90-91 1.7186206915158841 1105 92-93 1.7475623540306628 1105 94-95 1.8888288474847563 1105 96-97 2.047724806457392 1105 98-99 2.405692987897048 1105 100-101 2.9992270935648833 1106 1 0.13490562182820298 1106 2 0.056022979211601154 1106 3 0.2544299596954822 1106 4 0.17765246213502195 1106 5 -0.12876479692150156 1106 6 0.10815944271339362 1106 7 0.08912152338071166 1106 8 0.15252673777457915 1106 9 0.060900995097895816 1106 10-11 -0.13625066136561514 1106 12-13 -0.14904474546253255 1106 14-15 -0.45397514700108843 1106 16-17 -0.11291545512892043 1106 18-19 0.15644994954431013 1106 20-21 0.19652490831062863 1106 22-23 0.06153838299565706 1106 24-25 -0.019776643691059803 1106 26-27 -0.06487762571664746 1106 28-29 0.1416058996510401 1106 30-31 0.26992838674541275 1106 32-33 0.15657788019307617 1106 34-35 0.026021291398116375 1106 36-37 -0.12220990757337802 1106 38-39 -0.31690110219769707 1106 40-41 0.07402662099645596 1106 42-43 0.043586113792606795 1106 44-45 0.025394824244166614 1106 46-47 0.21060512378092966 1106 48-49 0.21978229136386318 1106 50-51 0.1870478052578477 1106 52-53 0.2712754435996274 1106 54-55 0.30041001500860887 1106 56-57 0.1768464973769568 1106 58-59 -0.0058727365085431416 1106 60-61 0.24620244260548674 1106 62-63 0.13744400271046686 1106 64-65 0.46299599942265246 1106 66-67 0.6422206536579651 1106 68-69 0.42125648277566796 1106 70-71 0.5348559857429542 1106 72-73 0.3656957305778086 1106 74-75 0.5384376947704475 1106 76-77 0.767914750432297 1106 78-79 0.8889762285682643 1106 80-81 0.7944561704816877 1106 82-83 0.7218491869784494 1106 84-85 0.7963587172045976 1106 86-87 0.9923424291982776 1106 88-89 1.3753589355206977 1106 90-91 1.5861850561548927 1106 92-93 1.7140238948430415 1106 94-95 1.7622409543528086 1106 96-97 1.9280988548250981 1106 98-99 1.9646411328507654 1106 100-101 2.2713285625252055 1107 1 0.22596512882896036 1107 2 0.26786545238827486 1107 3 0.26224298325009343 1107 4 0.3755303977960054 1107 5 0.385667690097236 1107 6 0.35536849944273996 1107 7 0.3501137149788818 1107 8 0.3741337572482095 1107 9 0.3306109126232428 1107 10-11 0.2823792749239189 1107 12-13 0.5051735015639593 1107 14-15 0.6241989949181672 1107 16-17 0.48535278508053636 1107 18-19 0.7347049716245948 1107 20-21 0.6713480696157177 1107 22-23 0.5650428048296092 1107 24-25 0.5640926169949481 1107 26-27 0.6821552278047562 1107 28-29 0.6266904425279307 1107 30-31 0.47106694832641693 1107 32-33 0.49491808063721976 1107 34-35 0.3882656234319697 1107 36-37 0.49087095641161227 1107 38-39 0.6883480202525547 1107 40-41 0.4912215121433121 1107 42-43 0.42931457829430286 1107 44-45 0.45342881239869115 1107 46-47 0.3032435270678988 1107 48-49 0.15031952050198072 1107 50-51 0.009346581194385806 1107 52-53 0.26182286363803087 1107 54-55 0.2444548087526499 1107 56-57 0.4032006045870773 1107 58-59 0.34150928822229076 1107 60-61 0.40696389465856697 1107 62-63 0.6176208529596963 1107 64-65 0.626215052939898 1107 66-67 0.6379773337815564 1107 68-69 0.6455348584247318 1107 70-71 0.7416665574732626 1107 72-73 0.7005731046993908 1107 74-75 0.6816521958900594 1107 76-77 0.9675177443847716 1107 78-79 1.0694643518943323 1107 80-81 1.1016713741032689 1107 82-83 1.175576491427556 1107 84-85 1.183768911340092 1107 86-87 1.4847662689723684 1107 88-89 1.666424104132723 1107 90-91 1.9457014159006043 1107 92-93 2.0602383566780027 1107 94-95 2.1081261183893325 1107 96-97 2.4559799794752912 1107 98-99 2.6131688828340103 1107 100-101 3.0723801201615615 1108 1 0.10996556221182274 1108 2 0.06282007985589999 1108 3 0.14205331191350368 1108 4 0.10838081879650474 1108 5 0.20259328091305662 1108 6 0.1775433296274116 1108 7 0.03526659299487278 1108 8 0.13968468415686175 1108 9 0.21154228777359663 1108 10-11 0.21669579034881536 1108 12-13 0.22241495330810324 1108 14-15 0.3704903652924685 1108 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-1.5083200515479902 2316 78-79 -1.5448291198188677 2316 80-81 -1.6117830357947085 2316 82-83 -1.5319086279058745 2316 84-85 -1.7856534795888557 2316 86-87 -2.29093376402788 2316 88-89 -2.21011045096634 2316 90-91 -2.2066649985789972 2316 92-93 -2.3988243141069994 2316 94-95 -2.284257721617095 2316 96-97 -2.557744805455645 2316 98-99 -3.1188289590546496 2316 100-101 -3.0787732297126382 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6246.0 3 1131.0 4 1021.0 5 1232.0 6 1481.0 7 1735.0 8 2134.0 9 2644.0 10 3439.0 11 4690.0 12 6155.0 13 7876.0 14 8665.0 15 11059.0 16 14304.0 17 19008.0 18 16273.0 19 12128.0 20 12331.0 21 13288.0 22 14859.0 23 16492.0 24 18598.0 25 21484.0 26 25462.0 27 29543.0 28 36062.0 29 43105.0 30 52264.0 31 65234.0 32 82745.0 33 108140.0 34 147150.0 35 204020.0 36 288288.0 37 357842.0 38 202554.0 39 13224.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83210841184125 18.675703841441578 15.002491592547809 23.489696154169362 2 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21.79724907800516 20-21 22.5159754785159 25.826787481362164 29.52978926214754 22.127447777974396 22-23 22.754593943781213 25.459621599267972 29.399769945271782 22.386014511679033 24-25 22.531331757756853 25.844132340939353 29.25450425344166 22.37003164786213 26-27 22.14639923223542 26.00326674205302 29.30361186185584 22.54672216385572 28-29 22.170771592800808 26.13426716181177 29.39618835225594 22.29877289313148 30-31 22.232505989135745 25.901859178515945 29.213695517979705 22.651939314368605 32-33 22.15644004161868 25.98847038518666 29.268400920914882 22.586688652279776 34-35 22.722063290032665 25.856034972032475 29.007089525205792 22.414812212729064 36-37 22.54790054521989 25.667036277064515 28.989546833415492 22.795516344300104 38-39 22.298541309603106 26.03443741038885 29.057453776418246 22.60956750358979 40-41 22.52946382297196 26.00062598846439 29.053482455082925 22.416427733480734 42-43 22.332620954107007 25.992753245468776 28.694849083864277 22.979776716559943 44-45 22.460471120147318 26.107037184452604 28.6796655118058 22.752826183594273 46-47 22.58018899069963 26.365349406105988 28.212271163079528 22.84219044011485 48-49 22.588884394613533 26.488112344645632 27.98053553998986 22.94246772075098 50-51 22.650358402913632 26.60056961656475 27.936794695840046 22.81227728468157 52-53 22.73429199482188 26.579970772819898 27.69855473572822 22.98718249663 54-55 22.651046361553178 26.466408538130633 27.83289117861233 23.04965392170386 56-57 22.705840941084908 26.91212996820233 27.692812635892476 22.689216454820286 58-59 22.72769059488362 26.36048340674441 27.999632276502417 22.912193721869553 60-61 22.648605984892768 26.326867549387433 28.213547542662653 22.810978923057142 62-63 22.396841144235314 26.550643466659256 28.407839624033304 22.64467576507213 64-65 22.611360098530593 26.556459441659968 28.010095750868132 22.822084708941308 66-67 22.46609905610578 26.625140488291194 27.826905061320385 23.081855394282645 68-69 22.34640514840032 26.5168916517036 28.095992071489167 23.040711128406915 70-71 22.466700842949646 26.510496377545646 28.019135154726083 23.003667624778625 72-73 22.527041168868138 26.366947291606245 27.95011032984011 23.155901209685503 74-75 22.418929206870594 26.856865462259634 27.57593837678779 23.14826695408198 76-77 22.61486328087932 26.505062428645243 27.790336838554275 23.089737451921163 78-79 22.58712936074485 26.487662465411276 27.7443236007339 23.18088457310997 80-81 22.452756706359843 26.668293633472178 27.675614182196583 23.203335477971397 82-83 22.592817501766703 26.54823238613706 27.76135208268073 23.097598029415504 84-85 22.433137729275288 26.509098314825962 27.676228066705654 23.3815358891931 86-87 22.471698693148483 26.52316557684418 27.837821584498997 23.167314145508342 88-89 22.510691756655618 26.335320218111836 27.725756441783382 23.42823158344916 90-91 22.37795545823936 26.232312956626284 27.91686200885361 23.472869576280743 92-93 22.409072892163454 26.22432432938092 27.886469107675648 23.480133670779978 94-95 22.300152885581713 26.080890371415745 27.957534158701648 23.661422584300894 96-97 22.323783170879178 26.021221236846987 27.886499313848812 23.768496278425022 98-99 22.113072025052748 26.082492744357065 27.806793815717096 23.99764141487309 100-101 22.49051506738605 25.393000659589006 28.447314660300975 23.669169612723977 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2215.0 1 1294.0 2 358.0 3 339.0 4 308.0 5 321.0 6 506.5 7 957.0 8 1625.0 9 2099.0 10 2145.0 11 2097.0 12 2238.5 13 2431.0 14 2534.5 15 2623.0 16 2703.5 17 2790.0 18 2921.0 19 3014.5 20 3251.5 21 3709.0 22 4182.0 23 4924.0 24 6028.0 25 7461.5 26 9362.5 27 11105.5 28 12309.5 29 14097.5 30 16686.5 31 19707.0 32 23091.0 33 26001.0 34 29385.0 35 33065.5 36 38104.0 37 43448.0 38 47037.0 39 49294.5 40 50658.5 41 54703.0 42 58825.5 43 62414.0 44 67137.5 45 70300.0 46 72568.0 47 76756.0 48 81849.0 49 83070.5 50 80564.5 51 76405.5 52 72198.5 53 71498.5 54 70850.0 55 65068.5 56 57602.0 57 50502.5 58 44132.5 59 38808.5 60 32302.5 61 25426.5 62 19630.0 63 14738.0 64 11130.0 65 8360.5 66 5958.0 67 4338.0 68 3117.5 69 2052.5 70 1395.0 71 934.5 72 597.0 73 426.5 74 281.5 75 182.0 76 140.0 77 96.5 78 57.5 79 36.0 80 31.0 81 21.0 82 15.0 83 10.0 84 7.0 85 7.5 86 4.5 87 4.5 88 6.0 89 5.0 90 5.0 91 3.0 92 2.0 93 2.5 94 1.5 95 1.5 96 1.0 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3001216175615972 2 0.25230707820612225 3 0.25945791333294554 4 0.2541214692084506 5 0.2542281980909405 6 0.1918985307168392 7 0.18522797556122048 8 0.1909379707744301 9 0.19195189515808414 10-11 0.19611432157519024 12-13 0.19896931918179503 14-15 0.19429993057286193 16-17 0.18685559101919144 18-19 0.17986484921610305 20-21 0.18058526917290987 22-23 0.18584166663553742 24-25 0.1857349377530475 26-27 0.18901685088961193 28-29 0.19283240843862584 30-31 0.1945400705584642 32-33 0.19173843739310437 34-35 0.19277904399738086 36-37 0.1851212466787306 38-39 0.17743676713945783 40-41 0.17426158288538332 42-43 0.18221288463088084 44-45 0.18183933354216616 46-47 0.18664213325421164 48-49 0.19352614617481018 50-51 0.18816301982969272 52-53 0.19904936584366245 54-55 0.18789619762346796 56-57 0.169298689849603 58-59 0.1566779994951724 60-61 0.17148663194064595 62-63 0.16558986118307897 64-65 0.17178013636749317 66-67 0.17073952976321663 68-69 0.1702325675713896 70-71 0.17151331416126842 72-73 0.18034512918730758 74-75 0.1837337712063619 76-77 0.18205279130714597 78-79 0.17903770037680633 80-81 0.17879756039120404 82-83 0.17039266089512445 84-85 0.1665504211254881 86-87 0.17020588535076714 88-89 0.17647620719704873 90-91 0.17386134957604618 92-93 0.17031261423325703 94-95 0.17220705189745278 96-97 0.17759686046319267 98-99 0.1757824694608644 100-101 0.16249472359087191 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1873907.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.45459117999789 #Duplication Level Percentage of deduplicated Percentage of total 1 66.2220583079627 26.789862959466138 2 15.056081590663428 12.181752510459626 3 6.744048167307601 8.184831345199289 4 3.5225250223904125 5.700092388084681 5 2.0809226309504476 4.209143715615299 6 1.3785004435932915 3.3460003132207405 7 0.9365636102585388 2.6521808576950447 8 0.6530457620956311 2.113495946192714 9 0.5073381738431101 1.8471742571546748 >10 2.604035987332821 19.02369988448186 >50 0.19695604342048462 5.476094584472567 >100 0.09488097372621956 6.7501354905063415 >500 0.0022493856408464697 0.5930926939371438 >1k 5.29267209610934E-4 0.4590580273650673 >5k 2.64633604805467E-4 0.673385026148851 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7008 0.37397800424460764 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5572 0.2973466666168599 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3519 0.18778946874097807 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2433 0.12983568554896266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 5.336444124494972E-5 0.0 0.0 0.0 0.0 4 5.336444124494972E-5 0.0 0.0 0.0 0.0 5 5.336444124494972E-5 0.0 0.0 0.0 0.0 6 5.336444124494972E-5 0.0 0.0 0.0 0.0 7 5.336444124494972E-5 0.0 0.0 0.0 0.0 8 5.336444124494972E-5 0.0 0.0 0.0 0.0 9 5.336444124494972E-5 0.0 0.0 0.0 0.0 10-11 5.336444124494972E-5 0.0 0.0 0.0 0.0 12-13 5.336444124494972E-5 0.0 0.0 2.668222062247486E-5 0.0 14-15 5.336444124494972E-5 0.0 0.0 8.004666186742458E-5 0.0 16-17 5.336444124494972E-5 0.0 0.0 1.0672888248989945E-4 0.0 18-19 5.336444124494972E-5 0.0 0.0 1.0672888248989945E-4 0.0 20-21 5.336444124494972E-5 0.0 0.0 1.8677554435732403E-4 0.0 22-23 5.336444124494972E-5 0.0 0.0 3.201866474696983E-4 0.0 24-25 5.336444124494972E-5 0.0 0.0 4.5359775058207265E-4 0.0 26-27 5.336444124494972E-5 0.0 0.0 7.204199568068212E-4 0.0 28-29 5.336444124494972E-5 0.0 0.0 0.0018143910023282906 0.0 30-31 5.336444124494972E-5 0.0 0.0 0.004722753050178051 0.0 32-33 5.336444124494972E-5 0.0 0.0 0.009445506100356101 0.0 34-35 5.336444124494972E-5 0.0 0.0 0.015315594637300572 0.0 36-37 5.336444124494972E-5 0.0 0.0 0.020118394349346046 0.0 38-39 5.336444124494972E-5 0.0 0.0 0.023960634118982426 0.0 40-41 5.336444124494972E-5 0.0 0.0 0.030604507053978665 0.0 42-43 5.336444124494972E-5 0.0 0.0 0.03994328427184486 0.0 44-45 5.336444124494972E-5 0.0 0.0 0.049415472592823445 0.0 46-47 5.336444124494972E-5 0.0 0.0 0.05998163195932349 0.0 48-49 5.336444124494972E-5 0.0 0.0 0.07065452020831343 0.0 50-51 5.336444124494972E-5 0.0 0.0 0.08439586382888799 0.0 52-53 5.336444124494972E-5 0.0 0.0 0.12159087937661794 0.0 54-55 5.336444124494972E-5 0.0 0.0 0.17887760705307149 0.0 56-57 5.336444124494972E-5 0.0 0.0 0.21994154459106027 0.0 58-59 5.336444124494972E-5 0.0 0.0 0.2863535917204002 0.0 60-61 5.336444124494972E-5 0.0 0.0 0.3638387604080672 0.0 62-63 5.336444124494972E-5 0.0 0.0 0.41213357973474674 0.0 64-65 5.336444124494972E-5 0.0 0.0 0.4565594770711674 0.0 66-67 5.336444124494972E-5 0.0 0.0 0.5826596517329836 0.0 68-69 5.336444124494972E-5 0.0 0.0 0.7080127242173704 0.0 70-71 5.336444124494972E-5 0.0 0.0 0.7676741695292242 0.0 72-73 5.336444124494972E-5 0.0 0.0 0.8968694817832475 0.0 74-75 5.336444124494972E-5 0.0 0.0 1.0947181476988987 0.0 76-77 5.336444124494972E-5 0.0 0.0 1.288377704976821 0.0 78-79 5.336444124494972E-5 0.0 0.0 1.4845720732138787 0.0 80-81 5.336444124494972E-5 0.0 0.0 1.7477654974339707 0.0 82-83 5.336444124494972E-5 0.0 0.0 2.0239531630972083 0.0 84-85 5.336444124494972E-5 0.0 0.0 2.3202592231097916 0.0 86-87 5.336444124494972E-5 0.0 0.0 2.726549396528216 0.0 88-89 5.336444124494972E-5 0.0 0.0 3.1878849910908067 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5385 0.0 35.31763 1 GTATCAA 7910 0.0 32.819614 1 ATCAACG 9765 0.0 26.330336 3 TCAACGC 9870 0.0 26.004858 4 TATCAAC 9965 0.0 25.89796 2 CAACGCA 10080 0.0 25.50956 5 AACGCAG 10225 0.0 25.133018 6 GTACATG 5730 0.0 24.810408 1 GTATAGG 435 0.0 24.046436 1 TACATGG 5985 0.0 23.346737 2 ACATGGG 6070 0.0 22.236223 3 GGACCGT 195 4.2227657E-7 21.923943 6 GAGTACT 6055 0.0 21.847046 12-13 ACGCAGA 11865 0.0 21.460064 7 CGCAGAG 11785 0.0 21.404194 8 GTACACG 245 7.4414856E-9 21.347347 1 AGTACTT 6365 0.0 20.745699 12-13 GCAGAGT 12100 0.0 20.179562 9 CATGGGG 3720 0.0 20.06017 4 GTACTTT 6530 0.0 19.965843 14-15 >>END_MODULE