FastQCFastQC Report
Thu 26 May 2016
SRR937764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873907
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80470.42942365869811044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69720.37205688435978945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42770.22823971520464997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62500.033.2265971
GTATCAA89250.030.4559561
ATCAACG110450.024.4706523
TCAACGC112050.024.1212274
TATCAAC113050.024.033912
CAACGCA113700.023.812965
AACGCAG115700.023.5232356
GTACATG56600.022.7529261
GAGTACT72950.022.2044312-13
TACATGG58300.021.4282232
CTATACG1150.003723874520.652434
GTACTTT78250.020.57770514-15
ACATGGG58050.020.29313
ACGCAGA131650.020.2058777
CGCAGAG131800.019.9305978
AGAGTAC108200.019.84365710-11
ACGGCGA1456.1464054E-419.655948
AGTACTT75100.019.51307912-13
GCATAAG7200.019.1403541
CCTACGG1250.00603210119.0002352
Produced by FastQC (version 0.11.5)