##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937764_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1873907 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.248010707041495 33.0 31.0 34.0 31.0 34.0 2 32.466843872187894 34.0 31.0 34.0 31.0 34.0 3 32.58161050681811 34.0 31.0 34.0 31.0 34.0 4 36.19834121970834 37.0 35.0 37.0 35.0 37.0 5 36.038621447062205 37.0 35.0 37.0 35.0 37.0 6 36.12647212481729 37.0 35.0 37.0 35.0 37.0 7 36.08596211017943 37.0 35.0 37.0 35.0 37.0 8 36.08197845464049 37.0 35.0 37.0 35.0 37.0 9 37.75263500269757 39.0 38.0 39.0 35.0 39.0 10-11 37.71120711967029 39.0 37.0 39.0 35.0 39.0 12-13 37.63555421907277 39.0 37.0 39.0 35.0 39.0 14-15 38.90117439125848 40.0 38.0 41.0 35.5 41.0 16-17 38.871609690342154 40.0 38.0 41.0 35.0 41.0 18-19 38.840413905279185 40.0 38.0 41.0 35.0 41.0 20-21 38.74942006193477 40.0 38.0 41.0 35.0 41.0 22-23 38.60659894007547 40.0 38.0 41.0 34.0 41.0 24-25 38.54875161894374 40.0 38.0 41.0 34.0 41.0 26-27 38.47628564277736 40.0 38.0 41.0 34.0 41.0 28-29 38.3625326657086 40.0 38.0 41.0 34.0 41.0 30-31 38.448505448776274 40.0 38.0 41.0 34.0 41.0 32-33 38.38059546178118 40.0 38.0 41.0 34.0 41.0 34-35 38.2601030894276 40.0 38.0 41.0 34.0 41.0 36-37 38.113957096056524 40.0 38.0 41.0 34.0 41.0 38-39 37.98872409356494 40.0 37.5 41.0 33.0 41.0 40-41 37.81778471396926 40.0 37.0 41.0 33.0 41.0 42-43 37.62251114916589 40.0 37.0 41.0 33.0 41.0 44-45 37.33076614794651 40.0 37.0 41.0 32.5 41.0 46-47 36.98646624405586 40.0 36.5 41.0 31.5 41.0 48-49 36.667238822417545 39.5 36.0 41.0 31.0 41.0 50-51 36.412100493781175 39.0 36.0 41.0 30.5 41.0 52-53 36.18032191565537 39.0 35.5 40.5 30.0 41.0 54-55 35.96141404029122 39.0 35.0 40.0 29.5 41.0 56-57 35.66810305954351 38.5 35.0 40.0 29.0 41.0 58-59 35.4062461477544 38.0 35.0 40.0 28.0 41.0 60-61 35.046401982595725 38.0 34.0 40.0 28.0 41.0 62-63 34.672760974797576 37.5 34.0 40.0 27.0 41.0 64-65 34.25421512380284 37.0 34.0 39.5 26.0 41.0 66-67 33.80686795022378 36.0 33.0 39.0 26.0 40.0 68-69 33.34834279395936 36.0 33.0 39.0 25.5 40.0 70-71 32.91757275040864 35.0 32.5 38.0 25.0 40.0 72-73 32.47180036149072 35.0 32.0 37.0 24.0 39.0 74-75 31.980073183994726 35.0 31.5 37.0 22.5 39.0 76-77 30.74585291585975 33.5 30.5 35.5 21.5 37.5 78-79 31.311362570287642 34.0 31.0 36.0 22.0 37.5 80-81 31.130884296819424 34.0 31.0 35.0 21.5 37.0 82-83 30.810288877729793 34.0 31.0 35.0 20.0 37.0 84-85 30.44590606684323 34.0 31.0 35.0 18.5 36.0 86-87 30.067950277148228 34.0 30.0 35.0 17.5 36.0 88-89 29.677078424916495 34.0 30.0 35.0 12.5 35.0 90-91 29.199130479794356 34.0 29.0 35.0 7.0 35.0 92-93 28.791783957261487 34.0 29.0 35.0 2.0 35.0 94-95 28.35534741051717 33.5 29.0 35.0 2.0 35.0 96-97 27.838362576157728 33.0 28.0 35.0 2.0 35.0 98-99 27.067598872302625 33.0 26.5 35.0 2.0 35.0 100-101 24.660887119798367 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2624176437003811 1101 2 0.17290562802116938 1101 3 0.14106646686429514 1101 4 0.1020753587260117 1101 5 0.038161289570886936 1101 6 0.1593786348568642 1101 7 0.13399939592608945 1101 8 0.13781156805229955 1101 9 0.24775084833656535 1101 10-11 0.23527331409293595 1101 12-13 0.298219291703262 1101 14-15 0.28677911694046543 1101 16-17 0.12768837804996735 1101 18-19 0.35500182925910195 1101 20-21 0.3647996553144637 1101 22-23 0.29257625506032525 1101 24-25 0.24320191080654752 1101 26-27 0.29644011732887776 1101 28-29 0.2681300574796808 1101 30-31 0.3151806875945553 1101 32-33 0.2944510618601015 1101 34-35 0.22507900266544567 1101 36-37 0.2211920891987802 1101 38-39 0.3464603462082394 1101 40-41 0.19962173596955068 1101 42-43 0.04688278767622478 1101 44-45 0.09793101171400309 1101 46-47 0.12358410735980385 1101 48-49 0.26490844162981375 1101 50-51 0.342462007826299 1101 52-53 0.3717004871648726 1101 54-55 0.3833066086205221 1101 56-57 0.3184534234790277 1101 58-59 0.40827677846368715 1101 60-61 0.47549215815163137 1101 62-63 0.3363270235811129 1101 64-65 0.16465101554242523 1101 66-67 0.2701800381943542 1101 68-69 0.251182239771353 1101 70-71 0.3217811948774596 1101 72-73 0.2261036591845098 1101 74-75 0.21346468884421554 1101 76-77 0.22956192791994567 1101 78-79 0.3350819084748302 1101 80-81 0.457201978293039 1101 82-83 0.34071568921319084 1101 84-85 0.5486939961628075 1101 86-87 0.6496739895830075 1101 88-89 0.7902581774877007 1101 90-91 0.905747214879991 1101 92-93 1.2217069328262546 1101 94-95 1.323865159982546 1101 96-97 1.3576346051808734 1101 98-99 1.594721096712206 1101 100-101 2.124714223445096 1102 1 0.08992048214291515 1102 2 0.10080250907373056 1102 3 0.05392294185261193 1102 4 0.008500256876011747 1102 5 -0.09614045356676115 1102 6 -0.016244152827638914 1102 7 -0.08090075768373595 1102 8 0.07057053419135428 1102 9 0.05782231030143947 1102 10-11 0.0170571917393616 1102 12-13 0.018993351677877968 1102 14-15 0.003131885256763667 1102 16-17 0.04573753319589002 1102 18-19 0.06186752083727498 1102 20-21 0.03415783336829037 1102 22-23 0.0869861929485225 1102 24-25 -0.029402109763815076 1102 26-27 -0.14603967963899578 1102 28-29 -0.20739922856854065 1102 30-31 -0.10383353801153561 1102 32-33 -0.056854621703649855 1102 34-35 -0.10806732739533231 1102 36-37 -0.140592456316476 1102 38-39 -0.11506373046309903 1102 40-41 -0.1940869396465601 1102 42-43 -0.12020463635369794 1102 44-45 -0.12471975967449822 1102 46-47 -0.01612069461708643 1102 48-49 -0.12398823906210055 1102 50-51 -0.07627839155927063 1102 52-53 -0.19587114176720632 1102 54-55 -0.23845589722333216 1102 56-57 -0.1661421819440676 1102 58-59 -0.06106804221352746 1102 60-61 -0.08095945475159283 1102 62-63 0.014835004613637182 1102 64-65 0.06081410911085072 1102 66-67 0.15844892218554207 1102 68-69 -0.03638872129189252 1102 70-71 -0.04041742931274683 1102 72-73 -0.14708474661259885 1102 74-75 -0.03399073091134497 1102 76-77 -0.12890330950742168 1102 78-79 0.07389176621876814 1102 80-81 0.025421439990765293 1102 82-83 0.09934121515989247 1102 84-85 0.21530723332303126 1102 86-87 0.011729021974645093 1102 88-89 0.06424468653732518 1102 90-91 0.1274429338407863 1102 92-93 0.22566405564733216 1102 94-95 0.13287137116266834 1102 96-97 0.08559159442818398 1102 98-99 0.2466579163208351 1102 100-101 1.227028388942994 1103 1 0.11333203630086075 1103 2 0.2194611267999491 1103 3 0.11621495897206557 1103 4 0.03910064106025857 1103 5 -0.09898259892993622 1103 6 -0.025819034274121577 1103 7 0.11906861410987091 1103 8 0.07810250475974101 1103 9 0.09785656252851993 1103 10-11 0.08222119769949643 1103 12-13 0.10737962567571913 1103 14-15 0.13110571050572872 1103 16-17 0.09680459802923735 1103 18-19 0.06741258163473418 1103 20-21 0.08944043802140555 1103 22-23 0.07715675020591561 1103 24-25 0.11033182909486783 1103 26-27 0.16133339521891088 1103 28-29 0.056531968304724955 1103 30-31 0.17864389389896473 1103 32-33 0.22272620941460275 1103 34-35 0.2830499933785333 1103 36-37 0.1194404214460647 1103 38-39 0.10876960358362453 1103 40-41 0.2681718810628624 1103 42-43 0.32840905683252686 1103 44-45 0.3156893551655813 1103 46-47 0.43375165615007205 1103 48-49 0.4407296604136519 1103 50-51 0.4894874776801714 1103 52-53 0.2240733136951505 1103 54-55 0.15113714713002224 1103 56-57 0.3822935442416622 1103 58-59 0.3561990609526333 1103 60-61 0.34658295984493037 1103 62-63 0.39715182867789167 1103 64-65 0.4847235856396068 1103 66-67 0.3845838898475762 1103 68-69 0.28470676499509295 1103 70-71 0.3851972868745932 1103 72-73 0.47027883064777143 1103 74-75 0.44992553708926764 1103 76-77 0.34164545853955985 1103 78-79 0.4579712578285893 1103 80-81 0.5961359760709328 1103 82-83 0.5855521017751286 1103 84-85 0.5915054311099048 1103 86-87 0.6654729872905811 1103 88-89 0.8348740200837206 1103 90-91 0.8414157926842272 1103 92-93 1.0528232371893687 1103 94-95 0.8746677440030624 1103 96-97 1.0214501139919498 1103 98-99 1.4537117254186498 1103 100-101 2.145247154236287 1104 1 0.2026498431887589 1104 2 0.23718699485883832 1104 3 0.14786724848969612 1104 4 0.08246241889011685 1104 5 0.014675002337284582 1104 6 0.07545778586890606 1104 7 0.0753335590881079 1104 8 0.06832607708813043 1104 9 0.11950158854680382 1104 10-11 0.12956603005812894 1104 12-13 0.10164004687786132 1104 14-15 0.17806362766950912 1104 16-17 0.1701462421258526 1104 18-19 0.08742761374465857 1104 20-21 0.16910683226475243 1104 22-23 0.29112489385146034 1104 24-25 0.21428977959599393 1104 26-27 0.14174189886733046 1104 28-29 0.27176566811308334 1104 30-31 0.2580188389681197 1104 32-33 0.20901547044699242 1104 34-35 0.1835935940739688 1104 36-37 0.21797726577381837 1104 38-39 0.1606609420374383 1104 40-41 0.019018447468319266 1104 42-43 0.2954588840339838 1104 44-45 0.3484996121560755 1104 46-47 0.2526442806170479 1104 48-49 0.08944955704769342 1104 50-51 0.1703046668170458 1104 52-53 0.2132199889015638 1104 54-55 0.22270180995313638 1104 56-57 0.439696656332913 1104 58-59 0.4994473402912618 1104 60-61 0.38622989720407475 1104 62-63 0.3944805479886 1104 64-65 0.37609487168308675 1104 66-67 0.4656042347173184 1104 68-69 0.3532127268228322 1104 70-71 0.41338375575317343 1104 72-73 0.5661827827389843 1104 74-75 0.7549819024503499 1104 76-77 0.38180887212159575 1104 78-79 0.48686665030505694 1104 80-81 0.6005332218004682 1104 82-83 0.5046279805928862 1104 84-85 0.42028914349553403 1104 86-87 0.5470771902200227 1104 88-89 0.5771117234036183 1104 90-91 0.6188092369467206 1104 92-93 0.8037844638418647 1104 94-95 0.9675917924492872 1104 96-97 1.1005166886610276 1104 98-99 1.3514703954001916 1104 100-101 1.9400007660340712 1105 1 0.1258782920814454 1105 2 0.1328778575102305 1105 3 0.04909576676568861 1105 4 0.03134155315983378 1105 5 -0.00613619699663559 1105 6 -0.016405177752560007 1105 7 0.051030969789614744 1105 8 0.07901706983323464 1105 9 0.1234038578147647 1105 10-11 0.11808553110539322 1105 12-13 0.12483521346285897 1105 14-15 0.18148536329307063 1105 16-17 0.08748930574601843 1105 18-19 0.16985553098771078 1105 20-21 0.1847023579083853 1105 22-23 0.23285104225368514 1105 24-25 0.1853891907051235 1105 26-27 0.05605540519638197 1105 28-29 0.05576538841160783 1105 30-31 -0.0028789485714995067 1105 32-33 0.07675868063940072 1105 34-35 -0.03089543035329001 1105 36-37 0.12083087132101156 1105 38-39 0.12369833747461456 1105 40-41 0.1341145216737729 1105 42-43 0.0066007056928469865 1105 44-45 0.18612124977570232 1105 46-47 0.23421488030570004 1105 48-49 -0.018376175921950733 1105 50-51 0.00777100506937245 1105 52-53 0.04424994081025346 1105 54-55 -0.10240616782686374 1105 56-57 0.08490996665412354 1105 58-59 0.08022252534956209 1105 60-61 0.1465197676740928 1105 62-63 0.032931424603383164 1105 64-65 0.06021351931559593 1105 66-67 0.1912677668482985 1105 68-69 0.028780694282048103 1105 70-71 -0.1289704200381152 1105 72-73 0.1056025322652232 1105 74-75 0.22420010429508253 1105 76-77 0.04967014235658951 1105 78-79 0.09195309757591019 1105 80-81 0.08353918876533939 1105 82-83 0.13811849242491192 1105 84-85 0.2579465568856456 1105 86-87 0.27370151861124015 1105 88-89 0.2857912505563398 1105 90-91 0.32936346867830935 1105 92-93 0.6980491741882169 1105 94-95 0.46178825780316757 1105 96-97 0.4998870351005813 1105 98-99 0.8332773319968538 1105 100-101 1.4200956151904123 1106 1 -0.10884465643363939 1106 2 0.08292777363755732 1106 3 -0.03704520724396332 1106 4 0.06410028508646093 1106 5 -0.0012693745825984593 1106 6 -0.033540501392430144 1106 7 -0.21011696531262913 1106 8 -0.15768979006883654 1106 9 0.043759620580729575 1106 10-11 -0.02061211638488203 1106 12-13 -0.19736914384152726 1106 14-15 0.012880485095209337 1106 16-17 0.14859878505629354 1106 18-19 0.21653475025873803 1106 20-21 0.003309018825085275 1106 22-23 0.1071973786816045 1106 24-25 -0.012213731595501542 1106 26-27 -0.20694443520783778 1106 28-29 -0.14219237982815258 1106 30-31 -0.24342671864491905 1106 32-33 -0.16903877194027928 1106 34-35 -0.4783625264571185 1106 36-37 -0.2582192960847749 1106 38-39 -0.4461524065133773 1106 40-41 -0.3616782618803924 1106 42-43 -0.5037267542893602 1106 44-45 -0.43642156943283794 1106 46-47 -0.5421073877201366 1106 48-49 -0.4522665947420279 1106 50-51 -0.5789540215865259 1106 52-53 -0.471626443768038 1106 54-55 -0.526436029216228 1106 56-57 -0.5691500481509308 1106 58-59 -0.43723963007635547 1106 60-61 -0.45754601974432063 1106 62-63 -0.44139190626243874 1106 64-65 -0.33796309888375475 1106 66-67 -0.21195224782093192 1106 68-69 -0.6082475643581944 1106 70-71 -0.42010877939348745 1106 72-73 -0.2601252075189464 1106 74-75 -0.19724905935511217 1106 76-77 -0.308247591111666 1106 78-79 -0.23586003274253287 1106 80-81 -0.15960073938260422 1106 82-83 -0.37661737655714234 1106 84-85 -0.4367454402348372 1106 86-87 -0.2630292806580137 1106 88-89 -0.15251224628909554 1106 90-91 0.04644641434104102 1106 92-93 0.40306268889479924 1106 94-95 0.20629451259952702 1106 96-97 0.38052127552653303 1106 98-99 0.7096984135808597 1106 100-101 1.2472597509622751 1107 1 0.09783304074641563 1107 2 0.16990167707589166 1107 3 0.1738613669266087 1107 4 0.11176989656127745 1107 5 -0.19739105531856183 1107 6 0.11165094194871017 1107 7 0.0539232529251521 1107 8 -0.012975539677718473 1107 9 0.1787798059580581 1107 10-11 0.08431776051551054 1107 12-13 0.13701832759043953 1107 14-15 0.1508337317404198 1107 16-17 0.15809353824469952 1107 18-19 0.20107450747959632 1107 20-21 0.12578526894350972 1107 22-23 0.21445024934959633 1107 24-25 0.13493847793495917 1107 26-27 0.07734239819520639 1107 28-29 0.12983533202586983 1107 30-31 0.2079296416554044 1107 32-33 0.17493530943441016 1107 34-35 0.19422533828352329 1107 36-37 0.10478299860093898 1107 38-39 0.15938753788729088 1107 40-41 0.25230557133085796 1107 42-43 0.14902294009917938 1107 44-45 0.22728680905859022 1107 46-47 0.08919924283546266 1107 48-49 0.09155369878362762 1107 50-51 0.1027426824313693 1107 52-53 0.3527931255335446 1107 54-55 0.06351079785505931 1107 56-57 0.18881722542505486 1107 58-59 0.1315633897577797 1107 60-61 0.28943310624154606 1107 62-63 0.14030398895384621 1107 64-65 0.2854681148501541 1107 66-67 0.24607961434078618 1107 68-69 0.20186834846685997 1107 70-71 0.23657977883676296 1107 72-73 0.15890335242134057 1107 74-75 0.2400168214645042 1107 76-77 0.21411866198777574 1107 78-79 0.40334801182936886 1107 80-81 0.16639425991258605 1107 82-83 0.15189576877988387 1107 84-85 0.14853171296836365 1107 86-87 0.24126025645326976 1107 88-89 0.25634852198000146 1107 90-91 0.36389526919350246 1107 92-93 0.5361648590250603 1107 94-95 0.3845364783109595 1107 96-97 0.6500278372184525 1107 98-99 1.0491287630524795 1107 100-101 1.794378221911277 1108 1 0.10798215982941883 1108 2 0.16261376934221516 1108 3 0.10947084474447877 1108 4 0.06682190250481312 1108 5 -0.06149788861651473 1108 6 0.03608754422706539 1108 7 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-0.5855449488525686 2316 92-93 -0.5882740058363112 2316 94-95 -0.6227292504968567 2316 96-97 -1.0651071407726533 2316 98-99 -0.9997094187561757 2316 100-101 -1.5630288492208386 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 4.0 5 22.0 6 48.0 7 128.0 8 337.0 9 772.0 10 1285.0 11 2084.0 12 3396.0 13 4802.0 14 6108.0 15 8513.0 16 12246.0 17 17513.0 18 15974.0 19 9478.0 20 8050.0 21 8495.0 22 8833.0 23 9412.0 24 10875.0 25 13027.0 26 15686.0 27 19683.0 28 24556.0 29 31255.0 30 40109.0 31 51112.0 32 66649.0 33 89317.0 34 126050.0 35 186016.0 36 297748.0 37 452785.0 38 311684.0 39 19850.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.3597671688421 19.60795828557426 15.258203304972406 22.77407124061124 2 15.801317781512104 20.945863375290237 38.70186727516361 24.55095156803406 3 19.042407120524125 24.191702149573057 30.44692185898233 26.31896887092049 4 13.719898436741223 17.04151649015479 35.77356601665185 33.465019056452135 5 14.117173619150245 37.13661111191169 33.94225272956965 14.80396253936841 6 32.80979258842621 35.963470972679005 16.589563943141254 14.637172495753525 7 28.686162120105212 32.867853100500724 20.467824710617975 17.97816006877609 8 26.696575657169753 34.70198894608964 19.545847259228978 19.055588137511627 9 26.347518846986535 16.75584754206052 20.352397424205147 36.544236186747796 10-11 25.075950941001874 26.609271431293013 26.856588934242737 21.458188693462375 12-13 25.794245341847766 24.080010096921374 27.667779266996597 22.45796529423427 14-15 23.002208754223126 25.42876994429286 25.904087022461624 25.664934279022383 16-17 22.278613613162232 28.773839897070662 26.618343386304655 22.329203103462444 18-19 22.27461128006886 27.10246559727884 29.09890939091428 21.52401373173802 20-21 22.67150682665343 26.11613182098346 29.471744832315405 21.74061652004771 22-23 22.85888559243909 25.835162627277903 29.235821626697028 22.070130153585982 24-25 22.73969700575859 26.1891662135112 29.15661853346262 21.91451824726759 26-27 22.454619438329665 26.26282436128344 29.257981455539987 22.02457474484691 28-29 22.358233806154143 26.480179949409237 29.284791820093282 21.876794424343334 30-31 22.382663099772188 26.25979308383216 29.155226615735426 22.202317200660225 32-33 22.456290520287293 26.34020791853598 29.13821230189118 22.065289259285546 34-35 22.93827815361168 26.120132962841808 28.992527377292472 21.949061506254044 36-37 22.84320709961284 25.979598752338035 28.84260408078318 22.33459006726595 38-39 22.521581914150488 26.300851643117827 28.992073779541887 22.185492663189795 40-41 22.846197810243517 26.338873807504854 28.81196879034018 22.002959591911445 42-43 22.65152871614635 26.335646970199527 28.453997621341852 22.55882669231227 44-45 22.722072119907764 26.49755297354671 28.417685616201872 22.362689290343653 46-47 22.848705938982032 26.8001560376262 27.893673485397088 22.45746453799468 48-49 22.98882041339843 26.9995061119645 27.51119119337064 22.500482281266436 50-51 22.955915101443132 27.13531141086511 27.464516648905203 22.444256838786554 52-53 23.240405260780364 27.10578367254041 27.10212820343833 22.551682863240895 54-55 23.04492698943971 27.09712915315435 27.322673964076127 22.53526989332982 56-57 22.991722641518496 27.48479513657828 27.254954488136285 22.268527733766938 58-59 23.041751805185637 26.919212106043684 27.443998021246518 22.59503806752416 60-61 23.014562622371333 26.96222384568711 27.694330615126578 22.328882916814976 62-63 22.706329609740507 27.117114136400577 27.90578721355969 22.270769040299225 64-65 22.950418563994905 27.174267452973922 27.53639855126215 22.338915431769028 66-67 22.79187280905616 27.261331538865058 27.34177843404182 22.60501721803697 68-69 22.687038364230457 27.08779037593648 27.608787415810927 22.616383844022142 70-71 22.843236083754423 27.079091972013554 27.491412327292657 22.58625961693937 72-73 22.928017734080576 26.86636896407824 27.42754972768111 22.778063574160072 74-75 22.855169134639322 27.49643592142938 26.834236393331256 22.814158550600045 76-77 23.061043050695684 27.098409899744226 27.214877792761328 22.625669256798766 78-79 22.90401818233242 27.106281154827855 27.152921676475938 22.836778986363786 80-81 22.799824110801655 27.388205497924922 27.018203144553066 22.793767246720357 82-83 22.89537700560514 27.30974283908278 27.07605963911181 22.718820516200267 84-85 22.768159700987564 27.28723273682402 27.04597145421692 22.898636107971495 86-87 22.729623188343925 27.228432360837544 27.12506543814608 22.916879012672453 88-89 22.88224549030448 27.175681610666913 26.946027737769267 22.99604516125934 90-91 22.716550645003597 27.154245837842915 27.003748556400193 23.1254549607533 92-93 22.59065294777341 27.115155399526163 27.10013296059185 23.194058692108577 94-95 22.55405417664804 27.021671833233984 27.19606682722248 23.22820716289549 96-97 22.592930172094984 26.912781690873665 27.13210954439041 23.362178592640937 98-99 22.24840346592113 27.05019950338945 26.886290272228607 23.815106758460818 100-101 22.07753878340136 27.175639740994797 27.226234617729823 23.52058685787403 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 315.0 1 231.5 2 212.0 3 271.5 4 267.0 5 325.0 6 544.0 7 1032.5 8 1768.5 9 2310.5 10 2390.0 11 2369.0 12 2514.0 13 2770.5 14 2988.5 15 3048.0 16 3111.5 17 3239.0 18 3338.5 19 3450.5 20 3660.5 21 3989.5 22 4554.0 23 5229.5 24 6045.5 25 7474.5 26 9438.5 27 11044.5 28 12024.5 29 13697.5 30 16487.5 31 19547.0 32 22942.5 33 25942.5 34 29507.0 35 32973.0 36 37691.0 37 43446.5 38 46834.0 39 49310.0 40 50914.0 41 54579.5 42 58498.0 43 61479.5 44 66316.0 45 69811.0 46 72215.0 47 76570.0 48 81707.0 49 82531.0 50 80621.5 51 77027.0 52 72376.0 53 71817.0 54 71151.0 55 65462.5 56 57796.0 57 50294.0 58 44320.0 59 39189.5 60 32565.0 61 25822.5 62 19920.0 63 14947.0 64 11046.0 65 8053.0 66 5741.5 67 4240.5 68 3022.5 69 1933.0 70 1334.5 71 877.0 72 520.5 73 359.0 74 253.0 75 169.0 76 114.5 77 62.5 78 29.5 79 15.5 80 8.0 81 6.0 82 4.0 83 2.5 84 1.0 85 0.0 86 0.5 87 1.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04215790858351028 2 0.0 3 0.0 4 5.336444124494972E-5 5 0.013874754723686928 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0036554642252790556 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.0672888248989945E-4 22-23 2.9350442684722346E-4 24-25 5.336444124494972E-4 26-27 0.0017076621198383911 28-29 0.002508128738512637 30-31 4.269155299595978E-4 32-33 0.0 34-35 0.0 36-37 1.0672888248989945E-4 38-39 0.0 40-41 0.0 42-43 0.008618357261059379 44-45 0.0 46-47 0.0 48-49 2.668222062247486E-5 50-51 0.0 52-53 1.334111031123743E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.336444124494972E-5 74-75 2.668222062247486E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.334111031123743E-4 84-85 2.668222062247486E-4 86-87 0.0 88-89 0.0 90-91 0.006350368508149017 92-93 0.002321353194155313 94-95 0.0 96-97 0.0 98-99 2.134577649797989E-4 100-101 0.01064620602836747 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1873907.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.57182133974394 #Duplication Level Percentage of deduplicated Percentage of total 1 64.92256752827242 25.04181675617986 2 15.579372355885557 12.018495341931269 3 6.754213916603241 7.815669974448973 4 3.5887184998224595 5.536936352551432 5 2.1959351354842584 4.235060885978262 6 1.4605330614059655 3.3801252183204107 7 1.0236243853322913 2.763813983702965 8 0.7167380803696064 2.2116714546725995 9 0.5509616826094179 1.912643602798966 >10 2.8856260838936594 19.991251812512985 >50 0.2101821994410565 5.589279069124533 >100 0.10692692796396078 7.326796158804233 >500 0.0037637532949780245 0.9022883884175172 >1k 5.575930807374851E-4 0.4666026240800439 >5k 2.7879654036874253E-4 0.8075483764759367 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8047 0.42942365869811044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6972 0.37205688435978945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4277 0.22823971520464997 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.668222062247486E-5 0.0 14-15 0.0 0.0 0.0 8.004666186742458E-5 0.0 16-17 0.0 0.0 0.0 1.0672888248989945E-4 0.0 18-19 0.0 0.0 0.0 1.0672888248989945E-4 0.0 20-21 0.0 0.0 0.0 1.8677554435732403E-4 0.0 22-23 0.0 0.0 0.0 3.201866474696983E-4 0.0 24-25 0.0 0.0 0.0 4.5359775058207265E-4 0.0 26-27 0.0 0.0 0.0 7.204199568068212E-4 0.0 28-29 0.0 0.0 0.0 0.0018677554435732402 0.0 30-31 0.0 0.0 0.0 0.004856164153290425 0.0 32-33 0.0 0.0 0.0 0.00971232830658085 0.0 34-35 0.0 0.0 0.0 0.01563578128477027 0.0 36-37 0.0 0.0 0.0 0.020411898776193267 0.0 38-39 0.0 0.0 0.0 0.024494278531431924 0.0 40-41 0.0 0.0 0.0 0.03140497367265291 0.0 42-43 0.0 0.0 0.0 0.040877161993631485 0.0 44-45 0.0 0.0 0.0 0.050536125858967385 0.0 46-47 0.0 0.0 0.0 0.06134242521106971 0.0 48-49 0.0 0.0 0.0 0.0721754067837945 0.0 50-51 0.0 0.0 0.0 0.08602347928685895 0.0 52-53 0.0 0.0 0.0 0.12356536370268108 0.0 54-55 0.0 0.0 0.0 0.1815991935565639 0.0 56-57 0.0 0.0 0.0 0.22338355105135954 0.0 58-59 0.0 0.0 0.0 0.29051601813750627 0.0 60-61 0.0 0.0 0.0 0.36898842898820483 0.0 62-63 0.0 0.0 0.0 0.41752338830048663 0.0 64-65 0.0 0.0 0.0 0.46218942562250953 0.0 66-67 0.0 0.0 0.0 0.588636469152418 0.0 68-69 0.0 0.0 0.0 0.7149234193585914 0.0 70-71 0.0 0.0 0.0 0.7746649113323126 0.0 72-73 0.0 0.0 0.0 0.9057546612505316 0.0 74-75 0.0 0.0 0.0 1.1055511292716234 0.0 76-77 0.0 0.0 0.0 1.3030262440985598 0.0 78-79 0.0 0.0 0.0 1.502449161030937 0.0 80-81 0.0 0.0 0.0 1.7689244983875936 0.0 82-83 0.0 0.0 0.0 2.047646975009966 0.0 84-85 0.0 0.0 0.0 2.3485423769696148 0.0 86-87 0.0 0.0 0.0 2.763771094296569 0.0 88-89 0.0 0.0 0.0 3.235779577108149 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6250 0.0 33.226597 1 GTATCAA 8925 0.0 30.455956 1 ATCAACG 11045 0.0 24.470652 3 TCAACGC 11205 0.0 24.121227 4 TATCAAC 11305 0.0 24.03391 2 CAACGCA 11370 0.0 23.81296 5 AACGCAG 11570 0.0 23.523235 6 GTACATG 5660 0.0 22.752926 1 GAGTACT 7295 0.0 22.20443 12-13 TACATGG 5830 0.0 21.428223 2 CTATACG 115 0.0037238745 20.65243 4 GTACTTT 7825 0.0 20.577705 14-15 ACATGGG 5805 0.0 20.2931 3 ACGCAGA 13165 0.0 20.205877 7 CGCAGAG 13180 0.0 19.930597 8 AGAGTAC 10820 0.0 19.843657 10-11 ACGGCGA 145 6.1464054E-4 19.65594 8 AGTACTT 7510 0.0 19.513079 12-13 GCATAAG 720 0.0 19.140354 1 CCTACGG 125 0.006032101 19.000235 2 >>END_MODULE