FastQCFastQC Report
Thu 26 May 2016
SRR937759_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937759_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253077
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84420.6737016161018038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66770.5328483405249638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45910.36637812361091937No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18560.148115399133493No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18300.14604050668873503No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17410.1389379902432173No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15930.1271270640192103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66450.029.057951
GTATCAA91800.028.0277671
GTACATG74300.027.0760611
TACATGG76300.025.7919242
ACATGGG75950.024.722633
GAGTACT58100.022.9724912-13
TCAACGC111450.022.9062654
ATCAACG111900.022.8123253
TATCAAC113200.022.7174072
GTATAGG4400.022.6986771
CGCGCGT1050.002195259822.6209437
CAACGCA112650.022.5778545
CATGGGA46750.022.3718134
ATGGGAG17000.022.371815
AACGCAG114900.022.118916
GTGTAGG5050.021.6605951
GTACTTT60800.021.60078214-15
GTACTAG1353.7644422E-421.1373921
CCTATAC2751.2478267E-920.743113
GTACCTG9200.020.6778831
Produced by FastQC (version 0.11.5)