##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937759_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1253077 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45346774380186 31.0 31.0 34.0 30.0 34.0 2 31.585745329297403 33.0 31.0 34.0 30.0 34.0 3 31.588594316231166 33.0 31.0 34.0 28.0 34.0 4 35.20862883924931 37.0 35.0 37.0 33.0 37.0 5 35.19210710914014 37.0 35.0 37.0 33.0 37.0 6 35.25650538634098 37.0 35.0 37.0 33.0 37.0 7 35.18559753311249 37.0 35.0 37.0 32.0 37.0 8 35.17561889652431 37.0 35.0 37.0 32.0 37.0 9 36.754137215829516 39.0 37.0 39.0 33.0 39.0 10-11 36.6092199441854 39.0 37.0 39.0 32.0 39.0 12-13 36.47406703658275 39.0 37.0 39.0 32.0 39.0 14-15 37.53005641313343 40.0 37.0 41.0 32.0 41.0 16-17 37.443153932280296 40.0 37.0 41.0 32.0 41.0 18-19 37.38926698040105 40.0 37.0 41.0 32.0 41.0 20-21 37.293250135466536 40.0 37.0 41.0 32.0 41.0 22-23 37.186876784108236 39.5 37.0 41.0 31.0 41.0 24-25 37.21045314852958 40.0 37.0 41.0 31.0 41.0 26-27 37.1048032164025 39.5 36.5 41.0 31.0 41.0 28-29 36.97274429264921 39.0 36.0 41.0 31.0 41.0 30-31 36.699084334003416 39.0 36.0 41.0 30.0 41.0 32-33 36.521649108554385 39.0 35.0 40.5 30.0 41.0 34-35 36.48606709723345 39.0 35.0 41.0 30.0 41.0 36-37 36.31325768488289 39.0 35.0 41.0 30.0 41.0 38-39 36.13686908306512 39.0 35.0 41.0 30.0 41.0 40-41 35.88598146801833 39.0 35.0 40.5 28.5 41.0 42-43 35.57738630586947 38.5 35.0 40.0 27.0 41.0 44-45 35.28676928871889 38.0 35.0 40.0 26.5 41.0 46-47 34.8596411074499 38.0 34.0 40.0 24.5 41.0 48-49 34.48556792599338 38.0 34.0 40.0 23.5 41.0 50-51 33.56887565568596 37.5 32.5 39.5 22.5 40.5 52-53 33.662578596526785 37.5 33.0 39.5 22.0 40.5 54-55 33.91867259553882 38.0 33.0 40.0 22.0 41.0 56-57 33.65386564432991 38.0 33.0 40.0 20.0 41.0 58-59 33.36835804982455 37.5 33.0 40.0 18.5 41.0 60-61 33.00896114125469 37.0 32.5 40.0 17.5 41.0 62-63 32.60004732350845 36.0 32.0 39.5 13.5 41.0 64-65 32.17330299734174 36.0 31.0 39.0 9.5 41.0 66-67 31.6962560959941 35.0 31.0 39.0 7.5 40.0 68-69 31.16218716008673 35.0 30.0 38.0 4.5 40.0 70-71 30.67801539729801 35.0 30.0 37.5 2.0 40.0 72-73 30.191511375597827 34.5 29.0 37.0 2.0 39.0 74-75 29.628540384988312 34.0 29.0 36.5 2.0 39.0 76-77 29.112436027474768 34.0 28.5 36.0 2.0 38.5 78-79 28.52522111570159 34.0 27.5 35.5 2.0 37.5 80-81 27.933637757296637 33.5 26.0 35.0 2.0 37.0 82-83 27.409504364057433 33.0 26.0 35.0 2.0 36.5 84-85 26.853443563324518 33.0 25.0 35.0 2.0 36.0 86-87 26.281271222758058 32.0 24.0 35.0 2.0 35.5 88-89 25.750034116020004 32.0 22.0 35.0 2.0 35.0 90-91 25.129177616379522 31.5 19.5 34.0 2.0 35.0 92-93 24.44008787967539 31.0 15.0 34.0 2.0 35.0 94-95 23.62488498312554 31.0 4.5 34.0 2.0 35.0 96-97 22.711173774636354 30.5 2.0 34.0 2.0 35.0 98-99 21.599089281823865 30.0 2.0 34.0 2.0 35.0 100-101 18.86475172714845 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20130904994528365 1101 2 0.3473166433282273 1101 3 0.25719676663636903 1101 4 0.09818582476469118 1101 5 -0.034306372546211605 1101 6 0.12867524189322666 1101 7 0.22156712482706098 1101 8 0.1948712806051205 1101 9 0.28104255186221394 1101 10-11 0.2388557102011859 1101 12-13 0.17951643057080702 1101 14-15 0.3778676001499335 1101 16-17 0.3979017954175319 1101 18-19 0.4719157789978752 1101 20-21 0.5853904706967512 1101 22-23 0.43044227694274895 1101 24-25 0.27886588833641923 1101 26-27 0.3801908140498611 1101 28-29 0.47429999030266856 1101 30-31 0.6953907382131419 1101 32-33 0.49350549875124017 1101 34-35 0.44545056687722706 1101 36-37 0.3958452033262603 1101 38-39 0.3450097778983121 1101 40-41 0.3240073164458579 1101 42-43 0.2670814671047239 1101 44-45 0.41891071769689603 1101 46-47 0.4809116118415133 1101 48-49 0.6040227894001617 1101 50-51 0.45119272212701134 1101 52-53 0.396192301941646 1101 54-55 0.5738500674815796 1101 56-57 0.5792937940523757 1101 58-59 0.5469054877554385 1101 60-61 0.7707175609986194 1101 62-63 0.696420071760322 1101 64-65 0.5353532638899807 1101 66-67 0.8759611868174808 1101 68-69 0.7701990880924932 1101 70-71 0.6457853514834007 1101 72-73 0.7187612635004861 1101 74-75 0.8261998343818142 1101 76-77 1.0329248732232514 1101 78-79 1.0223910474311886 1101 80-81 1.0540892862116564 1101 82-83 0.8620282153786789 1101 84-85 1.1566942355783176 1101 86-87 1.1666389652263653 1101 88-89 1.1368585418668182 1101 90-91 1.050915987191896 1101 92-93 1.137797398870731 1101 94-95 1.2833795863015247 1101 96-97 1.569315780260748 1101 98-99 1.8102513826198816 1101 100-101 2.3824563499972875 1102 1 0.18794282575267474 1102 2 0.3050173882601861 1102 3 0.32836925101752357 1102 4 0.14847778864225347 1102 5 0.24709505406605814 1102 6 0.16854218341707394 1102 7 0.13565585539515013 1102 8 0.11694121968227478 1102 9 0.22227659160149926 1102 10-11 0.1198451385499979 1102 12-13 0.073170564785066 1102 14-15 0.30471936415226253 1102 16-17 0.46837074891895014 1102 18-19 0.4208003936084239 1102 20-21 0.5518627822858946 1102 22-23 0.5330562952339832 1102 24-25 0.47722652050793357 1102 26-27 0.38620863376539916 1102 28-29 0.37424133877040333 1102 30-31 0.4800596876894545 1102 32-33 0.5658676892134622 1102 34-35 0.6277205536806321 1102 36-37 0.7143445866226514 1102 38-39 0.7190055860387972 1102 40-41 0.49765782806547065 1102 42-43 0.5435601901113571 1102 44-45 0.6766419384250355 1102 46-47 0.5781314142088476 1102 48-49 0.788894719179325 1102 50-51 0.6664872591967921 1102 52-53 0.5999526126797647 1102 54-55 0.7348157477099733 1102 56-57 0.6840902818606196 1102 58-59 0.7647645918245374 1102 60-61 0.7715642706189101 1102 62-63 0.8567293723408014 1102 64-65 0.6940910484145988 1102 66-67 0.6788474747217457 1102 68-69 0.872147814002961 1102 70-71 0.9140039836951637 1102 72-73 0.9052182221885339 1102 74-75 0.7758029302626888 1102 76-77 0.8011199298191052 1102 78-79 0.865092237999793 1102 80-81 0.7415168702572892 1102 82-83 0.8098496272130618 1102 84-85 1.1623572710251153 1102 86-87 1.2793643351766555 1102 88-89 1.1407976822768617 1102 90-91 1.436160526705489 1102 92-93 1.590753982361477 1102 94-95 1.492448435725219 1102 96-97 1.4461993138430635 1102 98-99 1.7778021348832844 1102 100-101 1.694191544079235 1103 1 0.28677178100350176 1103 2 0.35944511912505206 1103 3 0.25136642530104325 1103 4 0.2576981589670311 1103 5 0.3069231038840172 1103 6 0.36758094743282044 1103 7 0.32010757612791707 1103 8 0.3290783968653699 1103 9 0.30749505805535193 1103 10-11 0.45148988061104944 1103 12-13 0.4327948238209274 1103 14-15 0.3818387198998465 1103 16-17 0.41542781854427346 1103 18-19 0.37017931042623076 1103 20-21 0.3864708337998195 1103 22-23 0.4921115813480341 1103 24-25 0.4212849934174372 1103 26-27 0.44957443685152754 1103 28-29 0.3171821081421484 1103 30-31 0.4830041225598265 1103 32-33 0.5153483646499737 1103 34-35 0.4879890104236466 1103 36-37 0.5724200357146216 1103 38-39 0.5835987840769334 1103 40-41 0.772908600145314 1103 42-43 0.753879115846722 1103 44-45 0.6978460273708222 1103 46-47 0.8188750727594467 1103 48-49 0.7377902825582581 1103 50-51 0.6977922587728003 1103 52-53 0.8247710474024288 1103 54-55 0.852688062527065 1103 56-57 0.7478111459335324 1103 58-59 0.8133111121995071 1103 60-61 1.0459788166304946 1103 62-63 0.899081200029535 1103 64-65 0.8753961847629483 1103 66-67 1.0297396406758317 1103 68-69 0.9005691056262002 1103 70-71 1.0534064527093925 1103 72-73 0.8894861209004112 1103 74-75 0.7802280624531548 1103 76-77 0.9393560043436118 1103 78-79 1.1195861666757523 1103 80-81 0.9680665692213744 1103 82-83 0.8950008016771207 1103 84-85 0.9182512013423505 1103 86-87 1.1540537935855575 1103 88-89 1.247016993983756 1103 90-91 1.0129095029645505 1103 92-93 0.9557200217902064 1103 94-95 1.1186097012523 1103 96-97 1.073234828148081 1103 98-99 1.2165382490717747 1103 100-101 1.4435127429708317 1104 1 0.24243095740846243 1104 2 0.28715050534316333 1104 3 0.3458298616036224 1104 4 0.32244370779994114 1104 5 0.19677509453271114 1104 6 0.12872535389355022 1104 7 0.2324096912964535 1104 8 0.25830472101109336 1104 9 0.2860286958941245 1104 10-11 0.3104256690592351 1104 12-13 0.44044878104077156 1104 14-15 0.565926244553502 1104 16-17 0.5104475017378292 1104 18-19 0.3096238899592052 1104 20-21 0.4093770807702697 1104 22-23 0.3521280780415381 1104 24-25 0.39417193067438205 1104 26-27 0.3764390956258481 1104 28-29 0.47879169593142024 1104 30-31 0.5849689455064464 1104 32-33 0.5503450351150079 1104 34-35 0.5144639785189113 1104 36-37 0.6444834097175445 1104 38-39 0.8513831891390993 1104 40-41 0.7676822632853799 1104 42-43 0.7619717134720148 1104 44-45 0.7779439903947107 1104 46-47 0.6575054657717132 1104 48-49 0.6508282328997126 1104 50-51 0.7840391338572275 1104 52-53 0.72461800004357 1104 54-55 0.7041304107153721 1104 56-57 0.9477564748105394 1104 58-59 0.9305512694107705 1104 60-61 0.9708799238796644 1104 62-63 0.8087745172793888 1104 64-65 0.9425391576959683 1104 66-67 0.9729412706381488 1104 68-69 0.7713291136997285 1104 70-71 0.7680978866662009 1104 72-73 0.8849201285639019 1104 74-75 0.9618363152498866 1104 76-77 0.797106986914013 1104 78-79 0.9457113349404374 1104 80-81 0.984125416963888 1104 82-83 1.0098753203249018 1104 84-85 0.9495337318524903 1104 86-87 1.1509539091259846 1104 88-89 1.3841119866492448 1104 90-91 1.445842815319427 1104 92-93 1.3773686740039892 1104 94-95 1.3587931355841754 1104 96-97 1.234033090114938 1104 98-99 1.393129949550307 1104 100-101 1.1571870397555664 1105 1 0.28954747647915013 1105 2 0.29989976658466944 1105 3 0.294526049542327 1105 4 0.30514069405978717 1105 5 0.33499625025049085 1105 6 0.10976860078707773 1105 7 0.2308322544056054 1105 8 0.13848647662204883 1105 9 0.25647679905091536 1105 10-11 0.17003664062851698 1105 12-13 0.2942867176972612 1105 14-15 0.17488202700943845 1105 16-17 0.2846300660512142 1105 18-19 0.4244902594095592 1105 20-21 0.45539344272014404 1105 22-23 0.491288740430079 1105 24-25 0.3661424498893595 1105 26-27 0.26064437939059815 1105 28-29 0.36008814246804377 1105 30-31 0.4234659320777254 1105 32-33 0.5110967546457985 1105 34-35 0.605957394445781 1105 36-37 0.42441928449428445 1105 38-39 0.37866417897426174 1105 40-41 0.5680957500334003 1105 42-43 0.6145553308633751 1105 44-45 0.5147450895656007 1105 46-47 0.48044712863623573 1105 48-49 0.4890729541439356 1105 50-51 0.40955177489353645 1105 52-53 0.5491845941969302 1105 54-55 0.5254180746813475 1105 56-57 0.5534413725781988 1105 58-59 0.7542556974977614 1105 60-61 0.882802990221812 1105 62-63 0.8737938290725893 1105 64-65 0.8059735580701144 1105 66-67 0.8829479509977354 1105 68-69 0.790946376330254 1105 70-71 0.9632785807903375 1105 72-73 0.9400850016033324 1105 74-75 0.9838355646826749 1105 76-77 0.8624670645245338 1105 78-79 0.9293112338266809 1105 80-81 0.902398717897217 1105 82-83 0.9952579460955135 1105 84-85 1.1387664663411563 1105 86-87 1.1676908830736181 1105 88-89 1.211133755693588 1105 90-91 1.3841818505758425 1105 92-93 1.2388913599757032 1105 94-95 1.58391165097677 1105 96-97 1.5564543908593294 1105 98-99 1.6402586147293334 1105 100-101 1.6649792354137993 1106 1 -0.04443317466067853 1106 2 -0.06799545020789921 1106 3 -0.10314033636210596 1106 4 0.08397108114775165 1106 5 0.060593801764731836 1106 6 -0.2467122336305394 1106 7 -0.1268199755972006 1106 8 -0.0789790790208258 1106 9 -0.015268517080848198 1106 10-11 -0.17780798622055016 1106 12-13 -0.2819320532181564 1106 14-15 -0.19498204152538534 1106 16-17 -0.16241030094739273 1106 18-19 -0.18122014902828454 1106 20-21 -0.2650401582427122 1106 22-23 -0.13347965709970566 1106 24-25 -0.1875001895428383 1106 26-27 -0.08419000869101723 1106 28-29 0.10018151656727525 1106 30-31 -0.010167466989479124 1106 32-33 0.03694999845166791 1106 34-35 0.2539775460362961 1106 36-37 0.09728636616866737 1106 38-39 0.23377240780408926 1106 40-41 0.09863220279862617 1106 42-43 0.26297262827813483 1106 44-45 0.15422370513530126 1106 46-47 0.20221720704747526 1106 48-49 0.11542202369930266 1106 50-51 0.12306354080006798 1106 52-53 0.1909036818269172 1106 54-55 -0.0033415065275903544 1106 56-57 -0.08118684249657093 1106 58-59 -0.06999118902137269 1106 60-61 -0.3340138411663176 1106 62-63 -0.0617451315656794 1106 64-65 0.07817825536113077 1106 66-67 0.26736111134497165 1106 68-69 -0.004603164314961106 1106 70-71 -0.10361253743623067 1106 72-73 0.275300865917643 1106 74-75 0.2571125029573551 1106 76-77 0.3952772166899301 1106 78-79 0.3720344200226755 1106 80-81 0.0697843433280525 1106 82-83 0.00693656493520578 1106 84-85 -0.08183142535775545 1106 86-87 0.03087783854740067 1106 88-89 0.3185458786094273 1106 90-91 0.12670503983085624 1106 92-93 0.1683354771279184 1106 94-95 0.30914660255188053 1106 96-97 0.23782371590657192 1106 98-99 0.4245432960290394 1106 100-101 0.49909305962218653 1107 1 0.1731855879471098 1107 2 0.2608336496526569 1107 3 0.19991530100223542 1107 4 0.09481100932491415 1107 5 0.17517993902826845 1107 6 -0.024910666913100954 1107 7 2.4384897942297812E-4 1107 8 0.014594867150051982 1107 9 0.1669935433816505 1107 10-11 0.2438099593549694 1107 12-13 0.18623509216185852 1107 14-15 0.22442733070037235 1107 16-17 0.3814336670004508 1107 18-19 0.42937820571784613 1107 20-21 0.4299305014316772 1107 22-23 0.2890139063960575 1107 24-25 0.19228496474853074 1107 26-27 0.3002046748989855 1107 28-29 0.16226013533938044 1107 30-31 0.2272798982925579 1107 32-33 0.28634355783059817 1107 34-35 0.4072143766688754 1107 36-37 0.3675401106360141 1107 38-39 0.42263722907564727 1107 40-41 0.5092120318129787 1107 42-43 0.38664719397903724 1107 44-45 0.4384163474113407 1107 46-47 0.5170870888522572 1107 48-49 0.5088499807702789 1107 50-51 0.49138332554267805 1107 52-53 0.43313950014174196 1107 54-55 0.46941827859502894 1107 56-57 0.5008787185375283 1107 58-59 0.550477000027648 1107 60-61 0.7643546277215378 1107 62-63 0.8877952086110952 1107 64-65 0.622643970090202 1107 66-67 0.4655595235155836 1107 68-69 0.7349392976122893 1107 70-71 0.600041201330896 1107 72-73 0.48919178683124187 1107 74-75 0.5134376416892259 1107 76-77 0.4103693544960372 1107 78-79 0.6351987733479625 1107 80-81 0.7225580306664803 1107 82-83 0.8786260800043841 1107 84-85 0.8551605024900581 1107 86-87 0.8378740204521442 1107 88-89 1.0905469004275687 1107 90-91 1.3393957466693358 1107 92-93 1.0542430954788422 1107 94-95 1.0893913111067555 1107 96-97 1.121874010893226 1107 98-99 1.1526185411123322 1107 100-101 1.3045259019972342 1108 1 -0.06936723876413353 1108 2 -0.14639993084825065 1108 3 -0.12023814291358548 1108 4 0.0646579940233849 1108 5 -0.01956810720492541 1108 6 -0.022503283317597322 1108 7 0.06115708315998347 1108 8 0.06017799689159631 1108 9 0.030622763446508827 1108 10-11 0.05918435393728316 1108 12-13 0.06303348628698302 1108 14-15 0.018528169355668922 1108 16-17 0.11448554361668783 1108 18-19 0.004110678965169257 1108 20-21 0.14872738111964168 1108 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-0.9036046905387636 2316 88-89 -0.9828769656792247 2316 90-91 -1.1651674408206816 2316 92-93 -1.0290455670781142 2316 94-95 -0.953204981489538 2316 96-97 -0.9031314714293295 2316 98-99 -1.3118939112049972 2316 100-101 -0.8386331070154291 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5909.0 3 1309.0 4 1302.0 5 1533.0 6 1615.0 7 1911.0 8 2441.0 9 3066.0 10 3715.0 11 4763.0 12 6231.0 13 7498.0 14 8595.0 15 10582.0 16 12864.0 17 16634.0 18 14465.0 19 10789.0 20 10869.0 21 11600.0 22 13090.0 23 14069.0 24 15724.0 25 17568.0 26 20152.0 27 23541.0 28 27529.0 29 32729.0 30 38416.0 31 47516.0 32 57533.0 33 73054.0 34 95411.0 35 130238.0 36 169318.0 37 202620.0 38 126444.0 39 10434.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76282376759689 19.13590430686385 14.858992614470894 22.242279311068362 2 15.007656967840735 20.33135098051616 40.97682576455605 23.684166287087063 3 19.020703269152108 25.557006235075725 29.708867031746934 25.713423464025237 4 12.423510802147941 16.725077569892953 37.112991075859995 33.73842055209911 5 13.308680364302457 38.05697349712426 33.53337517773195 15.100970960841329 6 31.138372953195915 36.407381490293304 17.408401143611172 15.045844412899614 7 28.41013961239926 33.56239533868282 20.2872218428069 17.74024320611102 8 26.601207408230813 34.80875239014529 19.27846602055105 19.31157418107285 9 27.065408363435424 17.01572292591488 20.406536118106274 35.51233259254343 10-11 25.128731535017334 27.07267068599902 26.40758941228361 21.391008366700028 12-13 26.110352090797758 24.541838945618828 27.468312068103113 21.879496895480305 14-15 23.029332006906163 25.684038970728768 26.351864128648504 24.934764893716558 16-17 21.583175684348546 29.22102407660881 26.850151291042216 22.345648948000427 18-19 21.48882761532642 27.282777846195184 30.00806081436616 21.22033372411223 20-21 22.462254921363336 26.197321511746225 29.99076038696827 21.349663179922175 22-23 22.16937719541634 26.073010362085274 30.127009308205473 21.630603134292915 24-25 21.886925826134274 26.385679792830217 30.174876466183182 21.552517914852327 26-27 21.605705058213616 26.536900607263895 30.25516758028785 21.602226754234636 28-29 21.339382112780815 26.635689664824575 30.508041491054787 21.51688673133982 30-31 21.517830685566736 26.503392503022198 30.349215667377543 21.62956114403352 32-33 21.31021602988107 26.686168808836353 30.216509769733424 21.78710539154916 34-35 21.916722484929068 26.60361702340368 29.899607106081362 21.58005338558589 36-37 21.9262019822753 26.268146070470237 30.007459917932906 21.798192029321555 38-39 21.56845814865271 26.808031288575062 29.956076392220073 21.667434170552156 40-41 21.60561887050117 26.68339844280947 30.095769296577796 21.615213390111567 42-43 21.562015598406013 26.671299196810434 29.681990665904884 22.08469453887867 44-45 21.632638862788156 26.705541876549884 29.54945113822744 22.11236812243452 46-47 21.86145117635228 27.06244009678081 29.062872739471928 22.01323598739498 48-49 21.989754055752908 27.205133208471217 28.703198698704302 22.101914037071573 50-51 21.854068946764396 27.31890577702068 28.6021905253182 22.224834750896726 52-53 22.031080754603476 27.544490789850517 28.328549463454593 22.095878992091414 54-55 21.888714880809353 27.291645759709194 28.438833005952034 22.38080635352942 56-57 21.944380153825797 27.682447781693842 28.23296202003389 22.14021004444647 58-59 22.213809999876084 27.10689860416181 28.40184342260371 22.277447973358395 60-61 22.168487556354446 27.259823830771378 28.71809445922714 21.853594153647034 62-63 21.977841434662373 27.412430254804317 28.81265075315617 21.79707755737714 64-65 21.899197305511176 27.33946010291406 28.66388002713356 22.097462564441205 66-67 21.894696448843195 27.592856114992887 28.26339478438834 22.24905265177558 68-69 21.73247346353975 27.449965300320134 28.547402641013424 22.270158595126695 70-71 21.99426607429959 27.402240644195196 28.452327748378835 22.15116553312637 72-73 21.991675370633075 27.34836211538162 28.291668215592953 22.368294298392357 74-75 21.70424786763145 28.045648297203225 28.032176708862988 22.217927126302335 76-77 21.940417005803027 27.60272833538281 28.19878214732883 22.25807251148533 78-79 21.971933914931828 27.489363879647332 28.28628334256823 22.252418862852615 80-81 21.830721298867218 27.994112629146002 27.966810386524603 22.208355685462173 82-83 22.04148835558341 27.714958624367135 28.008837141255956 22.234715878793498 84-85 21.827446876799904 27.627381565730953 27.884428985803833 22.660742571665306 86-87 21.863472653722265 27.595165494172303 28.057008766428808 22.484353085676624 88-89 21.995143351087748 27.510257922033798 27.898669917756745 22.595928809121705 90-91 21.831720392926115 27.41760172437847 28.220827258739522 22.529850623955895 92-93 22.140104291991996 27.278639327658006 28.137813161767706 22.4434432185823 94-95 21.85998376282695 27.16148871932196 28.12732469989011 22.851202817960985 96-97 21.83018590380074 27.203120727946008 28.07715799184882 22.88953537640443 98-99 21.868182957478645 27.147764399812434 27.963500927227713 23.020551715481204 100-101 22.162418148502645 26.36312591293019 28.609708481783358 22.864747456783807 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2508.0 1 1506.5 2 696.0 3 859.0 4 695.0 5 662.0 6 1186.0 7 2534.0 8 3932.5 9 4270.5 10 3414.0 11 2512.0 12 2357.0 13 2386.0 14 2420.5 15 2377.5 16 2358.5 17 2438.0 18 2428.5 19 2434.0 20 2597.5 21 2894.5 22 3311.0 23 3933.5 24 4635.0 25 5524.0 26 6908.5 27 8262.0 28 9075.0 29 10419.5 30 12669.0 31 14999.0 32 18111.0 33 21705.5 34 24977.0 35 27768.5 36 31814.5 37 35570.5 38 36758.0 39 36923.5 40 36918.5 41 38552.0 42 39811.5 43 41412.5 44 43768.5 45 45193.0 46 45541.5 47 46659.5 48 50881.0 49 50385.0 50 46249.5 51 42580.5 52 38929.0 53 38457.5 54 38449.0 55 35780.0 56 32243.0 57 29210.0 58 26549.0 59 23764.5 60 20433.5 61 17239.0 62 14211.0 63 11115.5 64 8669.5 65 6930.0 66 5280.5 67 4197.0 68 3209.0 69 2212.5 70 1651.5 71 1269.0 72 968.5 73 734.0 74 583.0 75 483.0 76 341.0 77 206.5 78 125.0 79 91.0 80 69.5 81 45.0 82 24.0 83 14.5 84 11.0 85 9.5 86 10.0 87 8.0 88 4.5 89 3.0 90 4.0 91 3.5 92 2.5 93 2.0 94 0.5 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3526519120532896 2 0.3102762240468862 3 0.3075629031575873 4 0.3097175991579129 5 0.3196132400482971 6 0.21163902936531437 7 0.2086862978093126 8 0.20988335114282683 9 0.20828728003147454 10-11 0.2157489124770465 12-13 0.2044168075864452 14-15 0.20748924447579836 16-17 0.21327500225445045 18-19 0.20613258403114892 20-21 0.19819213025217128 22-23 0.1963167466963323 24-25 0.20018721914136164 26-27 0.19691527336308945 28-29 0.21443215381018085 30-31 0.21918046536645394 32-33 0.22241250936694235 34-35 0.21862184047748062 36-37 0.1909699084733021 38-39 0.1810742675829179 40-41 0.18857580180627367 42-43 0.1995487906968207 44-45 0.20589317336444607 46-47 0.2092848244760697 48-49 0.20996315469839444 50-51 0.21483117158801893 52-53 0.24284221959225172 54-55 0.19922957647455025 56-57 0.17927868758264656 58-59 0.17967770536048464 60-61 0.17377224224848115 62-63 0.18147328536075596 64-65 0.17776242002686188 66-67 0.1769244826934019 68-69 0.18713933780605663 70-71 0.1816328924718912 72-73 0.17640575958221244 74-75 0.1832688653610273 76-77 0.18761815913946228 78-79 0.19041128358432882 80-81 0.18067524980507982 82-83 0.17716389336010477 84-85 0.1708195106924794 86-87 0.16850520758101856 88-89 0.17672497380448288 90-91 0.18414670447227105 92-93 0.17285450135945357 94-95 0.1790392769159437 96-97 0.1767648755822667 98-99 0.18398709736113583 100-101 0.15597604935690307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1253077.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.62112947097848 #Duplication Level Percentage of deduplicated Percentage of total 1 75.18456913922155 37.307432394770466 2 13.117250550745844 13.017855757636465 3 4.646570430478347 6.917042187803589 4 2.1451607146570644 4.257811902322197 5 1.2031613147831905 2.9851111687664695 6 0.7737726092408852 2.3037282494543283 7 0.5041514585442384 1.75115953581844 8 0.3642437531231256 1.445934914617419 9 0.26376278972418765 1.177938678467735 >10 1.580118409297086 15.000176229476764 >50 0.14443928737768025 4.9171936393421465 >100 0.06893408470057491 5.80356705153056 >500 0.0024159113164316866 0.7816991385798253 >1k 0.0011274252810014537 1.1250361694906654 >5k 3.221215088575582E-4 1.2083129819229506 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8442 0.6737016161018038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6677 0.5328483405249638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4591 0.36637812361091937 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1856 0.148115399133493 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1830 0.14604050668873503 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1741 0.1389379902432173 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1593 0.1271270640192103 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.990177778380738E-5 0.0 18-19 0.0 0.0 0.0 7.980355556761476E-5 0.0 20-21 0.0 0.0 0.0 1.1970533335142214E-4 0.0 22-23 0.0 0.0 0.0 4.3891955562188115E-4 0.0 24-25 0.0 0.0 0.0 6.384284445409181E-4 0.0 26-27 0.0 0.0 0.0 7.980355556761476E-4 0.0 28-29 0.0 0.0 0.0 0.001476365778000873 0.0 30-31 0.0 0.0 0.0 0.002753222667082709 0.0 32-33 0.0 0.0 0.0 0.004947820445192114 0.0 34-35 0.0 0.0 0.0 0.007581337778923402 0.0 36-37 0.0 0.0 0.0 0.009616328445897578 0.0 38-39 0.0 0.0 0.0 0.011531613779520333 0.0 40-41 0.0 0.0 0.0 0.013646408002062124 0.0 42-43 0.0 0.0 0.0 0.015920809335739146 0.0 44-45 0.0 0.0 0.0 0.018594228447254238 0.0 46-47 0.0 0.0 0.0 0.022265192003364517 0.0 48-49 0.0 0.0 0.0 0.02725291422634044 0.0 50-51 0.0 0.0 0.0 0.03315837733834393 0.0 52-53 0.0 0.0 0.0 0.04672498178483844 0.0 54-55 0.0 0.0 0.0 0.06396254978744323 0.0 56-57 0.0 0.0 0.0 0.08531000090178018 0.0 58-59 0.0 0.0 0.0 0.11268262046147204 0.0 60-61 0.0 0.0 0.0 0.15054940757830526 0.0 62-63 0.0 0.0 0.0 0.17700428624896952 0.0 64-65 0.0 0.0 0.0 0.20357887025298527 0.0 66-67 0.0 0.0 0.0 0.2506230662600941 0.0 68-69 0.0 0.0 0.0 0.3117126880471033 0.0 70-71 0.0 0.0 0.0 0.3688919356112992 0.0 72-73 0.0 0.0 0.0 0.42838548628695605 0.0 74-75 0.0 0.0 0.0 0.526144841857284 0.0 76-77 0.0 0.0 0.0 0.5997237200906249 0.0 78-79 0.0 0.0 0.0 0.6709084916569373 0.0 80-81 0.0 0.0 0.0 0.7622835627818562 0.0 82-83 0.0 0.0 0.0 0.8976702948023146 0.0 84-85 0.0 0.0 0.0 1.0276702868219592 0.0 86-87 0.0 0.0 0.0 1.2087046526270933 7.980355556761476E-5 88-89 0.0 0.0 0.0 1.4295210908826832 7.980355556761476E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6645 0.0 29.05795 1 GTATCAA 9180 0.0 28.027767 1 GTACATG 7430 0.0 27.076061 1 TACATGG 7630 0.0 25.791924 2 ACATGGG 7595 0.0 24.72263 3 GAGTACT 5810 0.0 22.97249 12-13 TCAACGC 11145 0.0 22.906265 4 ATCAACG 11190 0.0 22.812325 3 TATCAAC 11320 0.0 22.717407 2 GTATAGG 440 0.0 22.698677 1 CGCGCGT 105 0.0021952598 22.620943 7 CAACGCA 11265 0.0 22.577854 5 CATGGGA 4675 0.0 22.371813 4 ATGGGAG 1700 0.0 22.37181 5 AACGCAG 11490 0.0 22.11891 6 GTGTAGG 505 0.0 21.660595 1 GTACTTT 6080 0.0 21.600782 14-15 GTACTAG 135 3.7644422E-4 21.137392 1 CCTATAC 275 1.2478267E-9 20.74311 3 GTACCTG 920 0.0 20.677883 1 >>END_MODULE