FastQCFastQC Report
Thu 26 May 2016
SRR937759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937759_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253077
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86320.6888642916596506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76990.6144075743150661No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52830.4216021840637088No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21710.17325351913729164No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19740.15753221869047154No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17930.14308777513273327No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14510.11579495912860902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGT300.00395034831.669194-95
GTATCAA102000.027.489671
GTACATG70150.027.3020361
GTATAGG4200.027.1568661
GGTATCA74450.026.9380051
GTGTACG1109.444108E-525.9224641
TACATGG71700.025.8449942
ACATGGG70600.025.1035483
CATGGGG28050.022.8681964
GAGTACT66800.022.64776412-13
ATCAACG124450.022.5262093
TCAACGC124800.022.4249634
TATCAAC125750.022.255552
CAACGCA126650.022.0973975
GTACCTG8700.021.8503511
GTACTTT70250.021.80511714-15
AACGCAG129200.021.6534846
AGTACTT70100.021.47996712-13
CATGGGA43850.021.3465484
GCGTCGC2257.153176E-821.111057
Produced by FastQC (version 0.11.5)