##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937759_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1253077 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08429011146163 31.0 31.0 34.0 30.0 34.0 2 32.356622138942775 34.0 31.0 34.0 31.0 34.0 3 32.43945822962196 34.0 31.0 34.0 31.0 34.0 4 36.09871939234381 37.0 35.0 37.0 35.0 37.0 5 36.00183947195583 37.0 35.0 37.0 35.0 37.0 6 36.04830668825619 37.0 35.0 37.0 35.0 37.0 7 35.989539349936194 37.0 35.0 37.0 35.0 37.0 8 35.96199515273204 37.0 35.0 37.0 35.0 37.0 9 37.63713802104739 39.0 37.0 39.0 35.0 39.0 10-11 37.54748511065162 39.0 37.0 39.0 35.0 39.0 12-13 37.43409503167004 39.0 37.0 39.0 34.5 39.0 14-15 38.60563277436263 40.0 38.0 41.0 34.0 41.0 16-17 38.56596522001442 40.0 38.0 41.0 34.0 41.0 18-19 38.558460892666616 40.0 38.0 41.0 34.0 41.0 20-21 38.46424162282126 40.0 38.0 41.0 34.0 41.0 22-23 38.403312007163166 40.0 38.0 41.0 34.0 41.0 24-25 38.422225050814916 40.0 38.0 41.0 34.0 41.0 26-27 38.273134452232384 40.0 38.0 41.0 34.0 41.0 28-29 38.11315186536821 40.0 37.5 41.0 33.5 41.0 30-31 38.135993638060555 40.0 37.0 41.0 34.0 41.0 32-33 38.038426210041365 40.0 37.0 41.0 33.5 41.0 34-35 37.88923306388993 40.0 37.0 41.0 33.0 41.0 36-37 37.72773500750553 40.0 37.0 41.0 33.0 41.0 38-39 37.58773722604437 40.0 37.0 41.0 33.0 41.0 40-41 37.39925160225589 40.0 36.0 41.0 33.0 41.0 42-43 37.15758768216159 40.0 36.0 41.0 32.0 41.0 44-45 36.82928583000087 39.0 36.0 41.0 31.0 41.0 46-47 36.41314061306687 39.0 35.0 41.0 30.5 41.0 48-49 36.05693026047082 39.0 35.0 41.0 29.5 41.0 50-51 35.784567109603 39.0 35.0 40.0 28.5 41.0 52-53 35.52970527748894 39.0 35.0 40.0 28.0 41.0 54-55 35.289786661154906 38.0 35.0 40.0 27.5 41.0 56-57 35.007804388716735 38.0 34.0 40.0 27.0 41.0 58-59 34.729375768607994 38.0 34.0 40.0 26.0 41.0 60-61 34.3841292274936 37.5 34.0 40.0 26.0 41.0 62-63 33.99162701094984 37.0 33.0 40.0 25.5 41.0 64-65 33.56068661383139 36.0 33.0 39.0 24.5 40.5 66-67 33.11822657346676 36.0 32.5 39.0 23.0 40.0 68-69 32.66025671207755 35.0 32.0 38.5 22.0 40.0 70-71 32.25291622142933 35.0 31.5 38.0 21.5 40.0 72-73 31.75025876302893 35.0 31.0 37.0 20.0 39.0 74-75 31.25844181961683 34.5 31.0 36.5 18.5 39.0 76-77 30.049149413802983 33.5 29.5 35.0 18.0 37.5 78-79 30.64355382789725 34.0 30.0 36.0 18.0 37.5 80-81 30.457916792024754 34.0 30.0 35.0 16.0 37.0 82-83 30.08526770501733 34.0 30.0 35.0 11.0 37.0 84-85 29.68582337717475 34.0 30.0 35.0 7.0 36.0 86-87 29.302007777654524 34.0 29.0 35.0 3.5 36.0 88-89 28.849763821377298 34.0 29.0 35.0 2.0 35.5 90-91 28.266397436071365 33.0 28.5 35.0 2.0 35.0 92-93 27.79146812207071 33.0 27.0 35.0 2.0 35.0 94-95 27.271322911521 33.0 26.0 35.0 2.0 35.0 96-97 26.630099746464104 33.0 25.0 34.5 2.0 35.0 98-99 25.65889087422401 32.0 23.5 34.0 2.0 35.0 100-101 22.84985000921731 29.5 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.278343923545016 1101 2 0.3706717200828322 1101 3 0.3388583188015062 1101 4 0.22556436310821226 1101 5 0.07015243376896763 1101 6 0.06988430945961 1101 7 0.18017360064314403 1101 8 0.12621368325305582 1101 9 0.12023290367611139 1101 10-11 0.25804150602473186 1101 12-13 0.2337355983769669 1101 14-15 0.2828475861549222 1101 16-17 0.3698644673678828 1101 18-19 0.3096794958005873 1101 20-21 0.30439926723487787 1101 22-23 0.2968908647797903 1101 24-25 0.20421231003419393 1101 26-27 -0.14223995825302183 1101 28-29 -0.057336033615669635 1101 30-31 -0.02229229465082483 1101 32-33 0.07721333107963346 1101 34-35 0.15023950112917106 1101 36-37 -0.04756364373597677 1101 38-39 -0.15473949904183115 1101 40-41 -0.25021620050320337 1101 42-43 -0.27737563988070235 1101 44-45 -0.19238119979006996 1101 46-47 -0.3237504986538795 1101 48-49 -0.21290719479285514 1101 50-51 -0.2850057708527558 1101 52-53 -0.2885865535430625 1101 54-55 -0.4083174855857834 1101 56-57 -0.4338494608846801 1101 58-59 -0.38674244552282744 1101 60-61 -0.1350432761387239 1101 62-63 -0.15692194882370103 1101 64-65 -0.34924136269894035 1101 66-67 -0.3775389033778467 1101 68-69 -0.20079423636894944 1101 70-71 -0.2921582287989004 1101 72-73 -0.12496213582229743 1101 74-75 -0.2387858723312668 1101 76-77 -0.14671117862046756 1101 78-79 -0.0906073666831162 1101 80-81 -0.11100548423835477 1101 82-83 -0.05852826871271688 1101 84-85 -0.2742099346971578 1101 86-87 -0.27931201366365244 1101 88-89 -0.33566237014631994 1101 90-91 -0.21896199111987968 1101 92-93 -0.26070948855707243 1101 94-95 -0.43209412322146434 1101 96-97 -0.4913122049841938 1101 98-99 -0.3616038185665431 1101 100-101 0.045461917195659396 1102 1 0.2676233434698645 1102 2 0.22340059762476017 1102 3 0.0972111307687129 1102 4 0.1286662816458346 1102 5 0.1836856998255385 1102 6 0.16030139660600895 1102 7 0.1910845106906649 1102 8 0.14698552591744374 1102 9 0.221250967773031 1102 10-11 0.17195801959365298 1102 12-13 0.26213559032674993 1102 14-15 0.2579109096842487 1102 16-17 0.1872072079469902 1102 18-19 0.27570530822493566 1102 20-21 0.23673581202710636 1102 22-23 0.2594257610039534 1102 24-25 0.255403597406584 1102 26-27 0.16633495326654213 1102 28-29 0.032874910645247724 1102 30-31 0.03828438791500588 1102 32-33 0.07305022231841463 1102 34-35 0.14878442561532523 1102 36-37 0.11174024307806008 1102 38-39 0.16049437394310218 1102 40-41 0.25745076229619457 1102 42-43 0.36071134264172144 1102 44-45 0.18067043368385072 1102 46-47 0.21560180442503452 1102 48-49 0.0899914559343955 1102 50-51 0.0691189556557319 1102 52-53 0.11421463213791583 1102 54-55 -0.17273210591773847 1102 56-57 0.09363884474730355 1102 58-59 -0.08884096284659648 1102 60-61 -0.08676932748181798 1102 62-63 -0.08564768678360934 1102 64-65 -8.57649239804914E-6 1102 66-67 0.15839062278102034 1102 68-69 0.2141984178141385 1102 70-71 0.020576053884234113 1102 72-73 -0.11217437674208952 1102 74-75 -0.23676958215568789 1102 76-77 -0.022340029424196217 1102 78-79 -1.1236500119338189E-4 1102 80-81 0.14351916521811248 1102 82-83 -0.005900593814711641 1102 84-85 0.13316147038079862 1102 86-87 0.18313059264943377 1102 88-89 0.03249099786042109 1102 90-91 0.17888429011421536 1102 92-93 0.0754825319594623 1102 94-95 -0.26076609727678957 1102 96-97 -0.30252557293342974 1102 98-99 -0.3210508841211315 1102 100-101 -0.4511630321311415 1103 1 0.3339891109951836 1103 2 0.2175719077122622 1103 3 0.23231758067858266 1103 4 0.14408982454173724 1103 5 0.12299598428464975 1103 6 -0.019769779718146197 1103 7 0.12015437673584017 1103 8 0.1422876696870503 1103 9 0.19921311466105607 1103 10-11 0.20219400658386633 1103 12-13 0.217986847674851 1103 14-15 0.20629767996963722 1103 16-17 0.23861008180733023 1103 18-19 0.16364609127391816 1103 20-21 0.1510698264866832 1103 22-23 0.17112280480666442 1103 24-25 0.19866879076163002 1103 26-27 0.15702130420794447 1103 28-29 0.1328918704150297 1103 30-31 0.16406351153258214 1103 32-33 0.1930570062263257 1103 34-35 0.17760454537317827 1103 36-37 0.30081760424729964 1103 38-39 0.22603130110636016 1103 40-41 0.24457063777866495 1103 42-43 0.42871454858529745 1103 44-45 0.39443027787280727 1103 46-47 0.2821150125296228 1103 48-49 0.24907437006051936 1103 50-51 0.3107893835616622 1103 52-53 0.42969698713871907 1103 54-55 0.2502396287110997 1103 56-57 0.39216990984819944 1103 58-59 0.15854983128094347 1103 60-61 0.19891662681980904 1103 62-63 0.3048573040389684 1103 64-65 0.21964971150090662 1103 66-67 0.0014793798213759146 1103 68-69 0.2785443314670317 1103 70-71 0.09268954209856162 1103 72-73 -0.09392730572168873 1103 74-75 0.05908566894464329 1103 76-77 -0.01261332790793901 1103 78-79 0.024186334840969437 1103 80-81 0.260678349070222 1103 82-83 0.39511070084487443 1103 84-85 0.3186094408160862 1103 86-87 0.10681792014532476 1103 88-89 0.173521057684642 1103 90-91 0.276450563150096 1103 92-93 0.2625367567201806 1103 94-95 0.2535173114181042 1103 96-97 0.009658380388902543 1103 98-99 0.22870415662334764 1103 100-101 0.5439987832207329 1104 1 0.2005868030473721 1104 2 0.08308730224229066 1104 3 0.038012595276093464 1104 4 0.08651191421827775 1104 5 0.06360947692709118 1104 6 0.01592537791427162 1104 7 0.03375969890608843 1104 8 -0.02101370328919927 1104 9 0.04086551756815737 1104 10-11 0.04874456068522903 1104 12-13 0.1427322057945446 1104 14-15 0.09585883295819286 1104 16-17 0.0857820431497558 1104 18-19 0.1180537121741807 1104 20-21 0.058833761663265705 1104 22-23 0.036389477512578594 1104 24-25 0.029983742519135603 1104 26-27 9.066410631106692E-4 1104 28-29 -0.05204671198181643 1104 30-31 0.1678785705662662 1104 32-33 0.2585344170400816 1104 34-35 0.1988372838401986 1104 36-37 0.22318063639679053 1104 38-39 0.0451414014374123 1104 40-41 0.046704082997081287 1104 42-43 0.18238776466179019 1104 44-45 0.1519233208134807 1104 46-47 0.028068316397764193 1104 48-49 0.11619334491339117 1104 50-51 0.3126099001719851 1104 52-53 0.12612365711108708 1104 54-55 -0.029616923162095077 1104 56-57 0.12459449468936157 1104 58-59 0.1335687834071635 1104 60-61 0.18808808912931596 1104 62-63 0.16806189005619387 1104 64-65 0.15337448371780482 1104 66-67 0.10605776851716797 1104 68-69 0.31332565492142095 1104 70-71 0.18117968143046426 1104 72-73 0.29367267965696797 1104 74-75 0.14968318935493485 1104 76-77 0.029270473705818034 1104 78-79 -5.769821069208092E-4 1104 80-81 0.18422936565114867 1104 82-83 0.2711677685973335 1104 84-85 0.14036330315611067 1104 86-87 0.05857065409879425 1104 88-89 -5.896319845533071E-5 1104 90-91 -0.20124155260647214 1104 92-93 0.011721060386484794 1104 94-95 -0.12772636127351333 1104 96-97 -0.1752833805616092 1104 98-99 0.03918527839850938 1104 100-101 -0.4362655770873971 1105 1 0.18584485334783096 1105 2 0.1449841817824833 1105 3 0.12448510069123131 1105 4 0.07234456887482565 1105 5 0.037736189904684636 1105 6 0.08304819842749112 1105 7 0.06622354736418856 1105 8 0.15913176971606902 1105 9 0.2134295350855453 1105 10-11 0.1303373411214821 1105 12-13 0.1893205767782149 1105 14-15 0.20713431448131558 1105 16-17 0.10146064466552929 1105 18-19 0.22425222422400282 1105 20-21 0.0029505034126060536 1105 22-23 0.055380229341110976 1105 24-25 0.03636408284894088 1105 26-27 0.07623275628608184 1105 28-29 0.03198748813725416 1105 30-31 0.049676952434730026 1105 32-33 -2.96815898913394E-4 1105 34-35 -0.09634922380588051 1105 36-37 -0.05309868879865576 1105 38-39 -0.013122026889909932 1105 40-41 0.1359566976545281 1105 42-43 0.10746626018459438 1105 44-45 0.009436752904015577 1105 46-47 -0.03084783548672476 1105 48-49 -0.203389192304833 1105 50-51 -0.13939558322496026 1105 52-53 -0.031624184489125184 1105 54-55 0.027668587724505755 1105 56-57 0.09516094454280477 1105 58-59 0.18629405348937667 1105 60-61 0.07346256558792419 1105 62-63 0.0481582595636425 1105 64-65 0.022015100615533356 1105 66-67 -0.21524105533467264 1105 68-69 0.11386553386520148 1105 70-71 -0.21337730483976358 1105 72-73 -0.1630804268140551 1105 74-75 0.0056254133281292695 1105 76-77 -0.11811813895426937 1105 78-79 0.1142104895456697 1105 80-81 0.0023208902109423946 1105 82-83 -0.14366335637625482 1105 84-85 -0.10002427189412444 1105 86-87 -0.21710499913085002 1105 88-89 -0.14272201302397391 1105 90-91 -0.018824653528692892 1105 92-93 0.08566283069734482 1105 94-95 0.06672738307582549 1105 96-97 -0.3091773554170878 1105 98-99 -0.10558491359824629 1105 100-101 -0.09696352154023202 1106 1 9.394536941371712E-4 1106 2 0.1052970250987002 1106 3 0.04409234298552178 1106 4 0.05201945781376338 1106 5 -0.0027758486769968727 1106 6 -0.10105130637965942 1106 7 -0.08525936771781062 1106 8 -0.015542246345916055 1106 9 -0.09241230594114569 1106 10-11 -0.2103183134070079 1106 12-13 -0.30291609259762 1106 14-15 -0.10916675475338877 1106 16-17 -0.03147587687501385 1106 18-19 -0.05146296789595084 1106 20-21 -0.014400233311363309 1106 22-23 0.04680783936184696 1106 24-25 0.07924844801424058 1106 26-27 0.10130937694958675 1106 28-29 0.020820728557097823 1106 30-31 -0.049679650233265704 1106 32-33 -0.0797890072103371 1106 34-35 -0.47392098035602004 1106 36-37 -0.07066988628561433 1106 38-39 -0.1354914632223725 1106 40-41 -0.28816999028114765 1106 42-43 -0.3772884561198424 1106 44-45 -0.2584040870831288 1106 46-47 -0.13900098082363854 1106 48-49 -0.3529879062485506 1106 50-51 -0.16379658131095454 1106 52-53 -0.0031805267180757824 1106 54-55 0.07024045766952725 1106 56-57 -0.14319458693905318 1106 58-59 -0.07313974209075269 1106 60-61 -0.10550489746248815 1106 62-63 -0.3643958205690936 1106 64-65 -0.585945273408278 1106 66-67 -0.7513351762579319 1106 68-69 -0.34772999025388884 1106 70-71 -0.5808948902775413 1106 72-73 -0.3121664903710979 1106 74-75 -0.40363757790248656 1106 76-77 -0.2510572975852803 1106 78-79 -0.049726612682192695 1106 80-81 -0.42321584999714545 1106 82-83 -0.6839006101313423 1106 84-85 -0.6965739554696384 1106 86-87 -0.47360450414896604 1106 88-89 -0.39150750100965226 1106 90-91 -0.3759784734333458 1106 92-93 -0.29506051266923805 1106 94-95 -0.13227590221147878 1106 96-97 -0.20441313503860314 1106 98-99 -0.5133083714145563 1106 100-101 -0.9880348078971402 1107 1 0.18819818374730346 1107 2 0.02700971052755108 1107 3 0.04191306245949278 1107 4 -0.0018540035212950556 1107 5 -0.06902354879449035 1107 6 -0.039522653775662775 1107 7 -0.0398037666660116 1107 8 -0.06733934788698548 1107 9 -0.10269910115844993 1107 10-11 0.06768668167918435 1107 12-13 0.07323516815495168 1107 14-15 -0.13970491934421858 1107 16-17 -0.0975734182834529 1107 18-19 0.011593397830495178 1107 20-21 0.04908259370475321 1107 22-23 -0.11565939753845811 1107 24-25 -0.08850521992231108 1107 26-27 0.037137508827456145 1107 28-29 0.1096294448347237 1107 30-31 0.15152325561484048 1107 32-33 0.11297281521498803 1107 34-35 0.13037730609296005 1107 36-37 0.2915073780496584 1107 38-39 0.15032389592875717 1107 40-41 -0.0556118875694267 1107 42-43 0.05639628553856113 1107 44-45 0.016863216530914826 1107 46-47 -0.18536967349811562 1107 48-49 -0.020272361206743028 1107 50-51 -0.00982690228641303 1107 52-53 -0.2098751772678824 1107 54-55 -0.37760934350873043 1107 56-57 -0.29657181200609983 1107 58-59 -0.18934252654518247 1107 60-61 -0.13440715021257432 1107 62-63 -0.2617429463361205 1107 64-65 -0.10603619199957137 1107 66-67 -0.04723386425610698 1107 68-69 -0.08366336517774897 1107 70-71 -0.04852869514616742 1107 72-73 -0.0036035382456027776 1107 74-75 -0.21140065121054974 1107 76-77 -0.09583198562990347 1107 78-79 -0.10677082426188633 1107 80-81 -0.06447956880292693 1107 82-83 -0.16141243018156715 1107 84-85 -0.012686011340289127 1107 86-87 -0.21599153677009042 1107 88-89 -0.3695109654798294 1107 90-91 -0.26147361715819173 1107 92-93 -0.5251939084939288 1107 94-95 -0.42584251198145395 1107 96-97 -0.46591571439048707 1107 98-99 -0.3707673799342821 1107 100-101 -0.07449218129672275 1108 1 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98-99 -0.3403601178197029 2316 100-101 0.18318103979113332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 13.0 5 29.0 6 60.0 7 179.0 8 329.0 9 637.0 10 1103.0 11 1818.0 12 2654.0 13 3907.0 14 5033.0 15 7212.0 16 10082.0 17 14376.0 18 12335.0 19 7513.0 20 6627.0 21 6790.0 22 7139.0 23 7877.0 24 9381.0 25 10974.0 26 13246.0 27 16418.0 28 20634.0 29 25561.0 30 31770.0 31 40021.0 32 50458.0 33 66352.0 34 90612.0 35 130957.0 36 191533.0 37 267032.0 38 180235.0 39 12175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99586778508651 19.878372240966318 15.451546447707965 21.674213526239207 2 15.644050605030657 21.03661626540109 39.81934071090603 23.49999241866222 3 19.318685124697048 25.59419732386757 30.02584837164835 25.06126917978704 4 12.98443750862876 17.426702429300033 36.63422120109139 32.95463886097981 5 13.345080092994738 38.331108527923966 33.55714449951459 14.766666879566706 6 31.322815756733224 36.81465704022977 16.920269065667952 14.942258137369052 7 28.49122599808312 33.81037238733134 20.065486797698785 17.632914816886753 8 26.783030891158326 35.12377930486315 18.973534746867113 19.119655057111416 9 26.991158564078667 16.991773051456533 20.479348036872437 35.53772034759237 10-11 25.388503659392043 27.227736204558855 26.19643485595857 21.18732528009053 12-13 26.255398463789643 24.710129978128816 27.36320333658282 21.671268221498725 14-15 23.13393350927357 25.83328877634814 26.242202195076597 24.790575519301687 16-17 21.674087067275195 29.312284879540524 26.86806157961562 22.145566473568664 18-19 21.56659167792562 27.264046822342124 30.1030982134378 21.066263286294458 20-21 22.49482673451033 26.222770029295884 30.211591147231974 21.070812088961812 22-23 22.263498658094647 25.971714247180323 30.37558187138936 21.389205223335665 24-25 22.056301498314145 26.378349260818386 30.41350278315344 21.151846457714026 26-27 21.7593470073097 26.530182277565785 30.489485152963365 21.220985562161154 28-29 21.455918669789146 26.653775603729894 30.640880816590048 21.249424909890912 30-31 21.634584307269225 26.525544719119416 30.505587445943068 21.33428352766829 32-33 21.47042839346664 26.70238939825725 30.3273462045828 21.49983600369331 34-35 22.018918230882857 26.548847357345156 30.131268868553168 21.300965543218815 36-37 22.07182798822419 26.267579725747105 30.09679373254796 21.56379855348075 38-39 21.686177305943687 26.782033346713728 30.236011035235666 21.29577831210692 40-41 21.67779932485815 26.7003838572466 30.27197203667792 21.34984478121733 42-43 21.662370105511837 26.68595463469919 29.8343895158587 21.817285743930277 44-45 21.765901057955737 26.787260479603408 29.67782506581798 21.769013396622874 46-47 22.078810799336352 27.196253701887436 29.04011485327717 21.68482064549904 48-49 22.15797592645943 27.285154862789756 28.72309522878482 21.833773981965994 50-51 22.16304345223797 27.345725761465577 28.6297649705485 21.861465815747955 52-53 22.303178495814702 27.659672949068575 28.25971588338147 21.777432671735255 54-55 22.10316852289885 27.443826232407293 28.38215718759277 22.070848057101085 56-57 22.22253700291363 27.75515790330522 28.1472726735867 21.875032420194447 58-59 22.43664994250154 27.255148725896333 28.272963273605694 22.035238057996438 60-61 22.38206431049329 27.461680327705317 28.577254230985005 21.579001130816383 62-63 22.14636450912434 27.55066129216321 28.743604742565697 21.559369456146747 64-65 22.10394891934015 27.500584561044533 28.516005002086864 21.87946151752845 66-67 22.18179728779636 27.71202408152093 28.121216812693874 21.98496181798884 68-69 21.98883229043387 27.58070733083442 28.38121679673316 22.04924358199855 70-71 22.26000477225262 27.550581488607644 28.378702984732783 21.81071075440695 72-73 22.352018271822082 27.553773630830346 28.101425530913104 21.992782566434464 74-75 22.084037932226032 28.236892066489126 27.76589148152907 21.913178519755768 76-77 22.17792681535133 27.776744765086264 28.14443964736405 21.900888772198357 78-79 22.145606375346446 27.693150540629187 28.13997064825226 22.021272435772104 80-81 22.10402872289572 28.206127795817814 27.86201486421026 21.827828617076207 82-83 22.287723756081583 28.00745211846299 27.835394210921766 21.86942991453367 84-85 22.074957943492652 27.928473612135257 27.721542827410307 22.27502561696178 86-87 22.05862049977775 27.89162198332584 27.9158822642184 22.13387525267801 88-89 22.18179728779636 27.703046181519568 27.86524690821075 22.249909622473318 90-91 22.068675162768795 27.769643494934865 28.057090294530212 22.10459104776613 92-93 22.254676276791056 27.647542582978303 27.988709181212133 22.109071959018507 94-95 22.024105461994754 27.570492475721764 28.012883486010836 22.392518576272646 96-97 22.13810484112309 27.60069812150411 27.953549542446314 22.30764749492649 98-99 22.015466881393575 27.608435160360862 27.757350080542544 22.618747877703026 100-101 21.93020299757153 27.398466293782764 28.221072397079457 22.45025831156625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1562.0 1 1004.0 2 702.5 3 944.5 4 727.0 5 665.5 6 1242.5 7 2576.0 8 3785.0 9 4122.5 10 3417.0 11 2562.5 12 2468.0 13 2552.0 14 2552.0 15 2494.0 16 2493.0 17 2498.0 18 2545.5 19 2543.5 20 2674.0 21 3026.0 22 3449.0 23 3999.5 24 4604.0 25 5480.0 26 6803.0 27 8220.5 28 9088.0 29 10356.5 30 12573.0 31 14778.0 32 17855.5 33 21608.0 34 24896.5 35 27635.0 36 31867.5 37 35754.0 38 36958.5 39 37096.0 40 36981.0 41 38339.5 42 39624.5 43 41344.5 44 43955.0 45 45830.0 46 45866.5 47 47025.0 48 51988.0 49 50976.0 50 46534.0 51 43109.5 52 39234.5 53 38727.0 54 38398.5 55 35589.0 56 32037.5 57 29052.5 58 26510.5 59 23694.0 60 20296.5 61 17023.0 62 13938.0 63 10776.5 64 8309.0 65 6566.5 66 4945.0 67 4044.5 68 3111.5 69 1996.5 70 1376.0 71 1079.0 72 918.5 73 739.5 74 563.5 75 417.5 76 292.0 77 171.5 78 98.0 79 73.5 80 47.0 81 28.0 82 21.0 83 14.0 84 6.5 85 5.0 86 3.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018993246225092315 2 0.0 3 0.0 4 0.0 5 0.04253529511753867 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0046685080007054635 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.384284445409181E-4 24-25 1.5960711113522952E-4 26-27 0.0012369551112980286 28-29 9.975444445951846E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 5.586248889733034E-4 42-43 0.010933087112763223 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 7.980355556761476E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.37937333459955E-4 84-85 7.980355556761476E-5 86-87 0.0 88-89 0.0 90-91 0.011930631557358407 92-93 0.0018753835558389467 94-95 0.0 96-97 0.0 98-99 9.975444445951846E-4 100-101 0.018314916002767585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1253077.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.24016141134001 #Duplication Level Percentage of deduplicated Percentage of total 1 73.06258655416109 34.51488381948569 2 13.996707489269031 13.22413442040761 3 5.212622394822299 7.387353699233133 4 2.386855636226168 4.510217820835632 5 1.3188456796530161 3.115124139172845 6 0.830337576768593 2.3535168691469552 7 0.5456557636444579 1.8043806444714527 8 0.3896745076332681 1.4726629310784023 9 0.2881836691044151 1.2252458740144292 >10 1.726010322905672 15.639739905959674 >50 0.16014683913191932 5.1679271749940074 >100 0.07798481570599244 6.2282160228024255 >500 0.003038366058982443 0.9628665579809952 >1k 8.439905719395674E-4 0.6702189167561297 >5k 5.063943431637404E-4 1.7235112036605573 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8632 0.6888642916596506 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7699 0.6144075743150661 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5283 0.4216021840637088 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2171 0.17325351913729164 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1974 0.15753221869047154 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1793 0.14308777513273327 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1451 0.11579495912860902 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.980355556761476E-5 0.0 12-13 0.0 0.0 0.0 7.980355556761476E-5 0.0 14-15 0.0 0.0 0.0 1.1970533335142214E-4 0.0 16-17 0.0 0.0 0.0 1.995088889190369E-4 0.0 18-19 0.0 0.0 0.0 2.3941066670284429E-4 0.0 20-21 0.0 0.0 0.0 2.793124444866517E-4 0.0 22-23 0.0 0.0 0.0 4.7882133340568857E-4 0.0 24-25 0.0 0.0 0.0 6.384284445409181E-4 0.0 26-27 0.0 0.0 0.0 7.980355556761476E-4 0.0 28-29 0.0 0.0 0.0 0.001476365778000873 0.0 30-31 0.0 0.0 0.0 0.0027931244448665166 0.0 32-33 0.0 0.0 0.0 0.0050276240007597305 0.0 34-35 0.0 0.0 0.0 0.00762123955670721 0.0 36-37 0.0 0.0 0.0 0.009576426668113772 0.0 38-39 0.0 0.0 0.0 0.01141190844616891 0.0 40-41 0.0 0.0 0.0 0.01356660444649451 0.0 42-43 0.0 0.0 0.0 0.01584100578017153 0.0 44-45 0.0 0.0 0.0 0.018554326669470433 0.0 46-47 0.0 0.0 0.0 0.02238489733671594 0.0 48-49 0.0 0.0 0.0 0.02745242311525948 0.0 50-51 0.0 0.0 0.0 0.03335788622726297 0.0 52-53 0.0 0.0 0.0 0.04680478534040606 0.0 54-55 0.0 0.0 0.0 0.06424186223192987 0.0 56-57 0.0 0.0 0.0 0.08546960801291541 0.0 58-59 0.0 0.0 0.0 0.1130018346837425 0.0 60-61 0.0 0.0 0.0 0.1509484253561433 0.0 62-63 0.0 0.0 0.0 0.17796192891578091 0.0 64-65 0.0 0.0 0.0 0.2043370040308776 0.0 66-67 0.0 0.0 0.0 0.25174031603804076 0.0 68-69 0.0 0.0 0.0 0.3129496431584013 0.0 70-71 0.0 0.0 0.0 0.3704481049448677 0.0 72-73 0.0 0.0 0.0 0.4305800840650654 0.0 74-75 0.0 0.0 0.0 0.529297082302205 0.0 76-77 0.0 0.0 0.0 0.603434585424519 0.0 78-79 0.0 0.0 0.0 0.6748188658797505 0.0 80-81 0.0 0.0 0.0 0.7668722672269941 0.0 82-83 0.0 0.0 0.0 0.9034959543587505 0.0 84-85 0.0 0.0 0.0 1.035171821045315 0.0 86-87 0.0 0.0 0.0 1.2179219632951526 0.0 88-89 0.0 0.0 0.0 1.4438857308848538 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGT 30 0.003950348 31.6691 94-95 GTATCAA 10200 0.0 27.48967 1 GTACATG 7015 0.0 27.302036 1 GTATAGG 420 0.0 27.156866 1 GGTATCA 7445 0.0 26.938005 1 GTGTACG 110 9.444108E-5 25.922464 1 TACATGG 7170 0.0 25.844994 2 ACATGGG 7060 0.0 25.103548 3 CATGGGG 2805 0.0 22.868196 4 GAGTACT 6680 0.0 22.647764 12-13 ATCAACG 12445 0.0 22.526209 3 TCAACGC 12480 0.0 22.424963 4 TATCAAC 12575 0.0 22.25555 2 CAACGCA 12665 0.0 22.097397 5 GTACCTG 870 0.0 21.850351 1 GTACTTT 7025 0.0 21.805117 14-15 AACGCAG 12920 0.0 21.653484 6 AGTACTT 7010 0.0 21.479967 12-13 CATGGGA 4385 0.0 21.346548 4 GCGTCGC 225 7.153176E-8 21.11105 7 >>END_MODULE