FastQCFastQC Report
Thu 26 May 2016
SRR937758_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937758_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198178
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80310.6702676897756427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61760.5154492905060851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42870.3577932494170315No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17440.1455543333294385No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16850.14063019017207795No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15300.12769388187731706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15240.12719312155622955No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.10223856555536823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG600.003936438431.6905864
GTATCAA93400.028.6149371
GGTATCA66550.028.5120321
GTACATG66500.025.601461
CCGTAAG950.001218219425.029361
GAGTACT56950.024.27234812-13
TCAACGC111150.023.8641644
ATCAACG111550.023.77663
CAACGCA112200.023.7245775
TATCAAC113150.023.5664122
AACGCAG113700.023.4804556
TACATGG70350.023.3098852
GTACTTT59100.023.18791814-15
ATACCGT1252.2546711E-422.8019496
ACATGGG70850.022.3403283
AGTACTT59500.021.51564212-13
ACGCAGA124300.021.4025637
CGCAGAG124500.021.1773958
CATGGGG28800.020.1367264
GCAGAGT129700.019.8155529
Produced by FastQC (version 0.11.5)