FastQCFastQC Report
Thu 26 May 2016
SRR937758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937758_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1198178
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83430.6963072264721936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72770.6073388094256447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52410.4374141404699469No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22170.18503093864183784No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18610.15531915959064513No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18460.15406725878792635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.123270499041044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78550.027.2870251
CTCGCGA350.008386848527.14717336-37
GTATCAA105850.025.4576341
GTATAGA3250.024.8594441
GAGTACT66150.023.48152212-13
GTACATG74600.022.2974431
GTACTTT69700.022.2155414-15
TACATGG77350.021.2545052
AGTACTT69200.021.21097612-13
GTATAGG4050.021.1224041
ATCAACG127350.021.1179943
TATCAAC128600.021.060522
TCAACGC127850.021.0354064
CAACGCA129200.020.8156095
ACATGGG76300.020.7374273
AACGCAG131450.020.5238976
TACCTGG8400.020.363782
GTACCTG8200.020.2852341
CGATCGT2000.020.18649924-25
TAGACTG4500.020.0620965
Produced by FastQC (version 0.11.5)