##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937758_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1198178 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.166042941866735 33.0 31.0 34.0 31.0 34.0 2 32.37124867924465 34.0 31.0 34.0 31.0 34.0 3 32.44805696649413 34.0 31.0 34.0 31.0 34.0 4 36.09316979614047 37.0 35.0 37.0 35.0 37.0 5 35.997169034984786 37.0 35.0 37.0 35.0 37.0 6 36.042742397206425 37.0 35.0 37.0 35.0 37.0 7 35.984670057370444 37.0 35.0 37.0 35.0 37.0 8 35.96369404212062 37.0 35.0 37.0 35.0 37.0 9 37.62205448606134 39.0 37.0 39.0 35.0 39.0 10-11 37.55091939594952 39.0 37.0 39.0 35.0 39.0 12-13 37.447704765068295 39.0 37.0 39.0 34.5 39.0 14-15 38.62109469544592 40.0 38.0 41.0 34.0 41.0 16-17 38.582841197217775 40.0 38.0 41.0 34.0 41.0 18-19 38.5809157737832 40.0 38.0 41.0 34.0 41.0 20-21 38.485903596961386 40.0 38.0 41.0 34.0 41.0 22-23 38.3239652205265 40.0 38.0 41.0 34.0 41.0 24-25 38.39372447165613 40.0 38.0 41.0 34.0 41.0 26-27 38.25814361472169 40.0 38.0 41.0 34.0 41.0 28-29 38.10196982418305 40.0 37.5 41.0 33.0 41.0 30-31 38.126558825149516 40.0 37.0 41.0 34.0 41.0 32-33 38.03056807920025 40.0 37.0 41.0 33.5 41.0 34-35 37.892406637411135 40.0 37.0 41.0 33.0 41.0 36-37 37.71625960416566 40.0 37.0 41.0 33.0 41.0 38-39 37.56961069223438 40.0 36.5 41.0 33.0 41.0 40-41 37.38426803029266 40.0 36.0 41.0 33.0 41.0 42-43 37.147657526678 40.0 36.0 41.0 32.0 41.0 44-45 36.806590506585835 39.0 36.0 41.0 31.0 41.0 46-47 36.39926121160629 39.0 35.0 41.0 30.0 41.0 48-49 36.04387328093155 39.0 35.0 41.0 29.5 41.0 50-51 35.771814788787644 39.0 35.0 40.0 28.5 41.0 52-53 35.51352887467472 39.0 35.0 40.0 28.0 41.0 54-55 35.277687038152926 38.0 35.0 40.0 27.5 41.0 56-57 34.99665867675755 38.0 34.0 40.0 27.0 41.0 58-59 34.72514058846015 38.0 34.0 40.0 26.0 41.0 60-61 34.35187551432258 37.5 34.0 40.0 26.0 41.0 62-63 33.984226884486276 37.0 33.0 40.0 25.5 41.0 64-65 33.57748890398589 36.0 33.0 39.0 24.5 41.0 66-67 33.12241586809306 36.0 32.5 39.0 23.0 40.0 68-69 32.66966928119194 35.0 32.0 38.5 22.0 40.0 70-71 32.24504831502498 35.0 31.5 38.0 21.5 40.0 72-73 31.738743325282222 35.0 31.0 37.0 19.5 39.0 74-75 31.214752315599185 34.5 31.0 36.5 18.0 39.0 76-77 29.992480249178335 33.5 29.5 35.0 17.5 37.5 78-79 30.60440977884755 34.0 30.0 36.0 18.0 38.0 80-81 30.405982667016087 34.0 30.0 35.0 14.5 37.0 82-83 30.045199461181895 34.0 30.0 35.0 9.5 37.0 84-85 29.634523000756147 34.0 30.0 35.0 7.0 36.0 86-87 29.199533374840797 34.0 29.0 35.0 2.0 36.0 88-89 28.812971444977293 34.0 29.0 35.0 2.0 35.5 90-91 28.254792693573076 33.0 28.5 35.0 2.0 35.0 92-93 27.741496255147396 33.0 27.0 35.0 2.0 35.0 94-95 27.22118333002275 33.0 26.0 35.0 2.0 35.0 96-97 26.6040951344458 33.0 25.0 34.5 2.0 35.0 98-99 25.66240032783109 32.0 23.5 34.0 2.0 35.0 100-101 22.991801301642994 29.5 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21912350857483887 1101 2 0.2363222654185435 1101 3 0.18485085021192305 1101 4 0.1470543949721872 1101 5 0.16732054375682281 1101 6 0.16732127417242282 1101 7 0.1703103257464207 1101 8 0.08359544836395827 1101 9 0.23144163706627552 1101 10-11 0.1842118805684123 1101 12-13 0.22291037280501058 1101 14-15 0.18784480975644868 1101 16-17 0.28532428299858736 1101 18-19 0.14365186321072798 1101 20-21 0.23141144902904642 1101 22-23 0.2729308864467086 1101 24-25 0.21400891216669748 1101 26-27 0.2213663055295001 1101 28-29 0.18254915096424895 1101 30-31 0.29182156012644356 1101 32-33 0.3674170096536997 1101 34-35 0.38731966778260585 1101 36-37 0.31798363567862253 1101 38-39 0.24108728526731227 1101 40-41 0.10887048387387921 1101 42-43 0.1496675598103252 1101 44-45 0.2359183287737565 1101 46-47 0.3509149412296253 1101 48-49 0.32976088420592475 1101 50-51 0.2887846434034387 1101 52-53 0.30292037688053597 1101 54-55 0.4750272241391613 1101 56-57 0.3297438623723892 1101 58-59 0.40687201914284543 1101 60-61 0.323482436014892 1101 62-63 0.32318363208861456 1101 64-65 0.4006955160972794 1101 66-67 0.1425183920915103 1101 68-69 0.2742557390739222 1101 70-71 0.15232444627307018 1101 72-73 -0.13195937040237737 1101 74-75 -0.060875903346438776 1101 76-77 0.23939644276103778 1101 78-79 0.211591963841812 1101 80-81 0.35118682396566925 1101 82-83 0.22066348956993664 1101 84-85 0.1566428611976569 1101 86-87 -0.1326815930774501 1101 88-89 0.314109016549164 1101 90-91 0.24461909254416625 1101 92-93 0.143754629114472 1101 94-95 -0.10399531340824808 1101 96-97 0.19821176573024601 1101 98-99 0.38217861116903507 1101 100-101 0.8580933516822071 1102 1 0.14966120487188306 1102 2 0.1398545448846562 1102 3 0.004365969264100045 1102 4 -0.04496724351797354 1102 5 -0.07912336135188269 1102 6 -0.016725662454149415 1102 7 0.137728384326266 1102 8 0.07327025552555 1102 9 0.18833821269092255 1102 10-11 0.12521976932404044 1102 12-13 0.13827828092349392 1102 14-15 0.21259289024426664 1102 16-17 0.14220106326399673 1102 18-19 0.24875274468643482 1102 20-21 0.03554274604649521 1102 22-23 0.1737907924159785 1102 24-25 0.017199513751542383 1102 26-27 0.13582207454592776 1102 28-29 0.08354546192993695 1102 30-31 -0.06296894453322466 1102 32-33 0.007705112531894542 1102 34-35 0.1676319354158693 1102 36-37 -0.05707021934282608 1102 38-39 0.06061460314029432 1102 40-41 0.18673971584166082 1102 42-43 0.3408280724491348 1102 44-45 0.14254273207121315 1102 46-47 0.13886505692434525 1102 48-49 0.24413347944405928 1102 50-51 0.21319964063375352 1102 52-53 0.1515995495565079 1102 54-55 0.23824395338024118 1102 56-57 0.12186181609782665 1102 58-59 0.10633493833176288 1102 60-61 -0.03425186590481388 1102 62-63 0.08069380840039031 1102 64-65 0.34288669047221276 1102 66-67 0.17691324463272196 1102 68-69 0.12380518671685792 1102 70-71 -0.06987846114952134 1102 72-73 -0.030134149562137225 1102 74-75 -0.15599094099920663 1102 76-77 -0.1544438735654765 1102 78-79 0.1410248351170864 1102 80-81 0.2501861325143224 1102 82-83 0.18643165763419844 1102 84-85 0.38738383420887246 1102 86-87 0.427027476135887 1102 88-89 0.3713966198302039 1102 90-91 0.5682546405734179 1102 92-93 0.47540896110981734 1102 94-95 0.31597681783110687 1102 96-97 0.5895493830676557 1102 98-99 0.7160445339294306 1102 100-101 1.1073291232825753 1103 1 0.26509133466657175 1103 2 0.27406536845963103 1103 3 0.21421379804054652 1103 4 0.06484615981376152 1103 5 0.16624130630810185 1103 6 0.16701315600004563 1103 7 0.24244897343168503 1103 8 0.08251456322447837 1103 9 0.17494671469997058 1103 10-11 0.1852378426825041 1103 12-13 0.2768552191029201 1103 14-15 0.34709686799681805 1103 16-17 0.3061546642193065 1103 18-19 0.25707724753459615 1103 20-21 0.27229916988826375 1103 22-23 0.35469946278697506 1103 24-25 0.27513027557587577 1103 26-27 0.24935315695721272 1103 28-29 0.264671980818207 1103 30-31 0.3449112549191966 1103 32-33 0.40715134697993705 1103 34-35 0.3580113683642452 1103 36-37 0.2713498679041564 1103 38-39 0.32865353616666937 1103 40-41 0.2937221642438388 1103 42-43 0.5145423627739731 1103 44-45 0.5021165020339353 1103 46-47 0.6002418595535843 1103 48-49 0.6148220959177095 1103 50-51 0.6845270104759891 1103 52-53 0.6290143062385383 1103 54-55 0.733775638236807 1103 56-57 0.599753418978807 1103 58-59 0.598204616507644 1103 60-61 0.5158113370600788 1103 62-63 0.5941323724290228 1103 64-65 0.6401426060007722 1103 66-67 0.6651494246993153 1103 68-69 0.6358793735502033 1103 70-71 0.6985652398009421 1103 72-73 0.7478698739117107 1103 74-75 0.7039203596909154 1103 76-77 0.526476899500917 1103 78-79 0.608264452349168 1103 80-81 0.5874387668676881 1103 82-83 0.5391448129951577 1103 84-85 0.5629837244777711 1103 86-87 0.712092481057649 1103 88-89 0.6465858253016954 1103 90-91 0.45959852387115774 1103 92-93 0.651764328520752 1103 94-95 1.0467343389839243 1103 96-97 1.0054550143173522 1103 98-99 1.1018292458045984 1103 100-101 1.5107974868367862 1104 1 0.028938948448526958 1104 2 0.07754860353015403 1104 3 -0.05155512397475803 1104 4 -0.014147040647692677 1104 5 -0.059156219489203465 1104 6 0.036921875251934466 1104 7 0.00452626169703052 1104 8 0.0707846946963997 1104 9 -0.008846034939850256 1104 10-11 -0.002457065903833211 1104 12-13 0.03928710410087177 1104 14-15 0.1210374637405991 1104 16-17 0.08972545863294101 1104 18-19 0.15731754397108233 1104 20-21 -0.06910172643870993 1104 22-23 0.02005770118017125 1104 24-25 0.009319089032480576 1104 26-27 0.17361698330582698 1104 28-29 -0.04493269964600444 1104 30-31 0.06349382492148692 1104 32-33 0.025681551497726218 1104 34-35 0.04211326043287045 1104 36-37 0.07181477771325007 1104 38-39 -0.05132929220744842 1104 40-41 0.05917264031786118 1104 42-43 0.08125147117850418 1104 44-45 0.11040407094638027 1104 46-47 -0.07518729818612968 1104 48-49 -0.03873982028687806 1104 50-51 0.0018990605979922748 1104 52-53 0.057808943162321214 1104 54-55 -0.05347801804359875 1104 56-57 0.1411000891187868 1104 58-59 0.11792008851470115 1104 60-61 -0.008626597879136 1104 62-63 -0.1000965258756068 1104 64-65 -0.09809881748979166 1104 66-67 -0.10163809902464749 1104 68-69 -0.17863942220314044 1104 70-71 -0.04820856788747818 1104 72-73 -0.01064576396368011 1104 74-75 0.04283004119180944 1104 76-77 -0.04526221937670627 1104 78-79 0.13835759379977475 1104 80-81 0.15447084006240885 1104 82-83 0.18539363676536524 1104 84-85 -0.02660856902412334 1104 86-87 -0.16241031265424155 1104 88-89 0.020656936034757933 1104 90-91 -0.09807076018368122 1104 92-93 -0.0606877583899994 1104 94-95 0.034067739656968854 1104 96-97 0.49854436525115986 1104 98-99 0.5570945022359801 1104 100-101 0.7714574514302761 1105 1 0.06768979054969293 1105 2 0.09174687592216202 1105 3 0.12031969538731602 1105 4 0.02340252639933027 1105 5 -0.04913455870279648 1105 6 -0.07922187417658932 1105 7 -0.0790243626401903 1105 8 0.09654011593616474 1105 9 0.10170017211036253 1105 10-11 0.04255343868423722 1105 12-13 4.251204100498285E-4 1105 14-15 0.12822409437952587 1105 16-17 0.11844018891358843 1105 18-19 0.04807744765888344 1105 20-21 0.12946730588851807 1105 22-23 0.07117127159454384 1105 24-25 0.16333610867221893 1105 26-27 0.022135200542180655 1105 28-29 0.11907418850508833 1105 30-31 0.13827871471001885 1105 32-33 0.19632198980021087 1105 34-35 0.03738856051516137 1105 36-37 0.015364571772877866 1105 38-39 0.07541573584857986 1105 40-41 -0.008847326281852474 1105 42-43 0.03497663247831895 1105 44-45 0.011222157124990417 1105 46-47 0.10076428927309422 1105 48-49 0.028735473699704528 1105 50-51 -0.00750540965862001 1105 52-53 0.03925013691232948 1105 54-55 0.12088763410792325 1105 56-57 0.049441646404268624 1105 58-59 0.08271868313850206 1105 60-61 -0.05029076845809044 1105 62-63 -0.05228908932090093 1105 64-65 0.2738156239803615 1105 66-67 0.29915896746798154 1105 68-69 0.15828223427423183 1105 70-71 0.2663557623359907 1105 72-73 0.09405276644497462 1105 74-75 0.22428300596596884 1105 76-77 0.26458056388195317 1105 78-79 0.5125660712493314 1105 80-81 0.20445670432358298 1105 82-83 0.3316638511346319 1105 84-85 0.4051762440425506 1105 86-87 0.4189040798400505 1105 88-89 0.48571381005011816 1105 90-91 0.317732522881073 1105 92-93 0.3971593633318484 1105 94-95 0.26480556216463924 1105 96-97 0.6908053898137112 1105 98-99 0.4016770460173582 1105 100-101 0.36966667928262353 1106 1 0.003583476443381528 1106 2 0.03083856501385185 1106 3 0.020577095644718213 1106 4 -6.214976246994297E-4 1106 5 -0.01772002728826294 1106 6 0.0715132765585551 1106 7 0.061112527496703706 1106 8 0.060991580387629085 1106 9 -0.006183685773493153 1106 10-11 -0.18530916417837062 1106 12-13 -0.25270463271970556 1106 14-15 0.005486096641526217 1106 16-17 0.07579364626704432 1106 18-19 0.115776640358078 1106 20-21 0.12621567763688546 1106 22-23 0.07531990074317463 1106 24-25 0.11170005703616681 1106 26-27 0.023617202024183825 1106 28-29 0.01450045893135865 1106 30-31 0.0018614532927543337 1106 32-33 0.027973446451667883 1106 34-35 -0.12793260480600566 1106 36-37 -0.1978583914500831 1106 38-39 -0.10272181728897323 1106 40-41 -0.12384869128322151 1106 42-43 -0.35357100606932335 1106 44-45 -0.2821166362138072 1106 46-47 -0.022295458786651068 1106 48-49 0.1176848126490384 1106 50-51 -0.022822674975884638 1106 52-53 0.052705150367337694 1106 54-55 0.3048898181101123 1106 56-57 0.14962299658561307 1106 58-59 0.09616882158863405 1106 60-61 0.09039212222480586 1106 62-63 -0.12753966457147925 1106 64-65 -0.20470022953549005 1106 66-67 -0.1350577167487117 1106 68-69 -0.06452011352811837 1106 70-71 0.22388471986495517 1106 72-73 0.13920993660214265 1106 74-75 0.022920929603895246 1106 76-77 -0.14884422454283808 1106 78-79 6.369343201910738E-4 1106 80-81 0.16218066204753967 1106 82-83 -0.1319978625270828 1106 84-85 -0.05303521416890433 1106 86-87 0.17838596432193654 1106 88-89 -0.21584814151183096 1106 90-91 -0.08310987796132707 1106 92-93 -0.044438078265596204 1106 94-95 0.2733026956617728 1106 96-97 0.24433294334126643 1106 98-99 0.3966216659619768 1106 100-101 0.537030471646414 1107 1 0.17722294324831722 1107 2 0.17581987726134685 1107 3 0.14978183995737737 1107 4 0.025001247422508754 1107 5 -0.018670618166915176 1107 6 0.07541285959483446 1107 7 0.06138798280812807 1107 8 -0.00767780842564747 1107 9 0.07511883977363709 1107 10-11 0.08449391227939174 1107 12-13 0.16775359529248135 1107 14-15 0.12545228585231882 1107 16-17 0.20775834477851163 1107 18-19 0.29702893581900724 1107 20-21 0.2519070164577286 1107 22-23 0.11278992472399096 1107 24-25 0.18536004796234806 1107 26-27 0.20173956899547107 1107 28-29 0.2152128619818896 1107 30-31 0.22499375825958623 1107 32-33 0.20203689839281935 1107 34-35 0.35192134986809265 1107 36-37 0.1274489302601154 1107 38-39 0.2025555824128844 1107 40-41 0.2755615102852573 1107 42-43 0.3436387936584637 1107 44-45 0.3914634186612105 1107 46-47 0.2713966145816826 1107 48-49 0.2786965438046636 1107 50-51 0.4511224932138873 1107 52-53 0.5071199968685178 1107 54-55 0.4416128720993484 1107 56-57 0.4160314914113741 1107 58-59 0.3315507862871456 1107 60-61 0.41091948411907 1107 62-63 0.4866122571344036 1107 64-65 0.5070627924793314 1107 66-67 0.4658723500087021 1107 68-69 0.39854456986031295 1107 70-71 0.4666526676688676 1107 72-73 0.3715294863677343 1107 74-75 0.3715366316368609 1107 76-77 0.36253856112052674 1107 78-79 0.6155044997273258 1107 80-81 0.4668234343162112 1107 82-83 0.5163213199647636 1107 84-85 0.6139339132318788 1107 86-87 0.6367895117542623 1107 88-89 0.7185664889459638 1107 90-91 0.6590153407825952 1107 92-93 0.7712445107982902 1107 94-95 1.0130433355822674 1107 96-97 1.0529131062379768 1107 98-99 1.2565256346788978 1107 100-101 1.2789963583964727 1108 1 0.14271947591148404 1108 2 0.17745104465215888 1108 3 0.03893973873061185 1108 4 0.028896581339878935 1108 5 -0.07261181944942763 1108 6 0.01725422045448255 1108 7 -0.028887250879463977 1108 8 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2316 78-79 0.16294347162673262 2316 80-81 0.1902856901525709 2316 82-83 0.34486361433439683 2316 84-85 0.25687084573715424 2316 86-87 0.3253309862669589 2316 88-89 0.49461231894862934 2316 90-91 0.7650074701560214 2316 92-93 0.8247965409690252 2316 94-95 0.35720390456297935 2316 96-97 0.5285787275870533 2316 98-99 0.6403365346768481 2316 100-101 1.2470578066737517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 10.0 5 40.0 6 50.0 7 156.0 8 337.0 9 559.0 10 1010.0 11 1763.0 12 2591.0 13 3787.0 14 4829.0 15 6812.0 16 9773.0 17 13739.0 18 12180.0 19 7388.0 20 6307.0 21 6634.0 22 6874.0 23 7768.0 24 8746.0 25 10648.0 26 12744.0 27 15684.0 28 19633.0 29 24194.0 30 30675.0 31 38222.0 32 48585.0 33 63680.0 34 87046.0 35 125472.0 36 181450.0 37 251679.0 38 173963.0 39 13146.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.029953436188876 19.86474957551635 15.47265857824273 21.632638410052042 2 15.600603583107016 21.050211237395445 39.937889028174446 23.411296151323093 3 19.414394188509554 25.555301466059298 30.005558439564073 25.024745905867075 4 13.034228694281975 17.43817268915418 36.60597441444329 32.92162420212056 5 13.30337382569778 38.377277747677276 33.55133703277319 14.768011393851763 6 31.408772319304813 36.77742372168409 16.916184406657443 14.897619552353659 7 28.412306017970618 33.80632927661833 20.1453373371903 17.63602736822075 8 26.81513097386198 35.17073423147479 19.003186504843185 19.010948289820043 9 26.970283213345596 17.05823341773927 20.42826691860475 35.54321645031039 10-11 25.32530669508762 27.214593503297092 26.252131362895465 21.20796843871982 12-13 26.250782984785996 24.71469307205046 27.403612016797712 21.630911926365837 14-15 23.103620664041568 25.833932854717744 26.335694696447437 24.726751784793244 16-17 21.655922575777556 29.35469521223057 26.88744911023237 22.101933101759506 18-19 21.5174206169701 27.28476069498856 30.168222083864 21.029596604177343 20-21 22.475500301290793 26.14987088729721 30.330593617976625 21.044035193435366 22-23 22.215712270132965 26.02186425831824 30.431271806774607 21.33115166477419 24-25 22.086427965150392 26.413584971168703 30.431981251517932 21.068005812162976 26-27 21.807798559469855 26.56484472153367 30.477186042047457 21.150170676949013 28-29 21.421456384781568 26.654456333453098 30.727391165911556 21.19669611585378 30-31 21.623206234799838 26.471901503783247 30.635014163171082 21.269878098245837 32-33 21.43754934575664 26.685392320673557 30.44046877842858 21.43658955514122 34-35 22.025817532954203 26.496981249864376 30.195596981416784 21.281604235764636 36-37 22.11048775724475 26.182879338462232 30.13967874556201 21.566954158731004 38-39 21.736971358584185 26.744660119222736 30.289293531217204 21.229074990975878 40-41 21.71346088602508 26.718253605598196 30.300253344366812 21.26803216400991 42-43 21.684509800402594 26.685108722009804 29.8636921200698 21.7666893575178 44-45 21.775780299663445 26.699257831164413 29.757277198077915 21.767684671094226 46-47 22.140533376510003 27.145424135645957 29.140912285152957 21.573130202691086 48-49 22.257627831590966 27.19721109885176 28.836283089824715 21.70887797973256 50-51 22.148128241379826 27.34176391153902 28.64674530829309 21.86336253878806 52-53 22.367544722069677 27.678900797711194 28.231197701843968 21.722356778375165 54-55 22.18034382203646 27.480015490185934 28.314323915144495 22.025316772633115 56-57 22.199330984211027 27.81076768226424 28.177699807541117 21.812201525983618 58-59 22.433728544506742 27.263979141663423 28.281816224300567 22.020476089529268 60-61 22.37284443546785 27.476885738179135 28.64382420642008 21.506445619932933 62-63 22.155597916169384 27.534181064916897 28.793426352344976 21.51679466656874 64-65 22.100347360742727 27.439453904177842 28.604931821482282 21.855266913597145 66-67 22.185434885300847 27.652235310613282 28.19293126730753 21.969398536778343 68-69 22.05028053022194 27.515998255684153 28.487348493858384 21.946372720235523 70-71 22.302112040114242 27.537686387164513 28.383428839454574 21.77677273326668 72-73 22.338375433366327 27.564769174529996 28.117650299037372 21.979205093066305 74-75 22.08194441894276 28.22009751472653 27.792323010437514 21.905635055893196 76-77 22.180761122304034 27.72275905583311 28.209331167823144 21.887148654039716 78-79 22.202711116378367 27.65970498540284 28.19418316811025 21.943400730108547 80-81 22.115671509438293 28.232085813662835 27.801473487859685 21.850769189039188 82-83 22.35087426449109 27.95196761674248 27.837499478362478 21.859658640403957 84-85 22.11419650692743 27.913700167296586 27.758672276881896 22.213431048894087 86-87 22.05836695382489 27.902406821023252 27.929823448602797 22.10940277654906 88-89 22.20325360672621 27.718168752889806 27.831298855428827 22.247278784955157 90-91 22.13718921414003 27.69701798325002 28.1113226154738 22.05447018713615 92-93 22.286377218021265 27.648772263675365 27.995643247032902 22.069207271270468 94-95 22.03729329031246 27.601741978236955 27.977938169453953 22.383026561996633 96-97 22.143287558275983 27.546282772676513 27.983655183119705 22.3267744859278 98-99 22.05278607060677 27.59575748420282 27.731215525177415 22.62024092001299 100-101 21.935076990371822 27.471238173467498 28.170604252960494 22.42308058320019 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1422.0 1 931.5 2 685.0 3 894.0 4 670.5 5 572.5 6 1118.0 7 2439.5 8 3572.5 9 3809.5 10 3219.5 11 2441.0 12 2296.5 13 2379.0 14 2439.5 15 2483.0 16 2479.5 17 2496.0 18 2500.0 19 2545.5 20 2677.5 21 2865.5 22 3240.5 23 3825.5 24 4460.0 25 5338.0 26 6675.5 27 8051.0 28 8677.0 29 9807.0 30 12157.5 31 14359.5 32 17326.0 33 20835.0 34 23777.5 35 26326.5 36 30317.0 37 34179.5 38 35372.5 39 35333.0 40 35306.0 41 37080.5 42 38329.5 43 39610.0 44 42142.0 45 43869.5 46 43939.5 47 45004.0 48 49702.0 49 48838.5 50 44601.5 51 41111.0 52 37163.0 53 36586.5 54 36444.0 55 34116.0 56 30478.0 57 27659.5 58 25471.5 59 22389.0 60 19071.0 61 15963.0 62 13205.0 63 10447.5 64 7983.5 65 6326.5 66 4715.0 67 3818.0 68 2947.5 69 1923.5 70 1355.0 71 1031.0 72 877.5 73 705.0 74 519.0 75 414.5 76 278.5 77 160.0 78 106.0 79 68.0 80 44.0 81 30.0 82 18.5 83 14.0 84 10.5 85 5.5 86 4.0 87 4.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021031933485675752 2 0.0 3 0.0 4 1.6692010702917264E-4 5 0.02829295814144476 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.346005351458632E-5 12-13 0.0032132120603115735 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001043250668932329 24-25 8.346005351458632E-5 26-27 6.676804281166906E-4 28-29 0.001043250668932329 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.173002675729316E-5 40-41 5.424903478448111E-4 42-43 0.015982600248043276 44-45 4.173002675729316E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.173002675729316E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.173002675729316E-5 82-83 0.002336881498408417 84-85 5.424903478448111E-4 86-87 0.0 88-89 0.0 90-91 0.012226897839886895 92-93 0.00317148203355428 94-95 0.0 96-97 0.0 98-99 0.0017526611238063126 100-101 0.01969657262944237 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1198178.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.85916805691296 #Duplication Level Percentage of deduplicated Percentage of total 1 73.51774666874478 35.18498192985008 2 13.963476064188468 13.365606952293547 3 4.955002323272477 7.114268667356749 4 2.3592626506022882 4.516493907422913 5 1.25929857538061 3.0134491076485843 6 0.8072401828035982 2.3180306142654326 7 0.5288556941407151 1.7717415480615086 8 0.3712289155314256 1.4213365644803233 9 0.29466731101400523 1.2692279122828123 >10 1.711792969885911 15.626568635231287 >50 0.15367334788357373 5.0608280774388295 >100 0.07357115716750963 6.015745226571851 >500 0.002963765397444357 0.9636169445279403 >1k 6.973565641045546E-4 0.6175191797083113 >5k 5.23017423078416E-4 1.7405847328597597 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8343 0.6963072264721936 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7277 0.6073388094256447 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5241 0.4374141404699469 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2217 0.18503093864183784 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1861 0.15531915959064513 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1846 0.15406725878792635 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1477 0.123270499041044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.346005351458631E-5 0.0 24-25 0.0 0.0 0.0 5.00760321087518E-4 0.0 26-27 0.0 0.0 0.0 7.094104548739837E-4 0.0 28-29 0.0 0.0 0.0 0.0017526611238063126 0.0 30-31 0.0 0.0 0.0 0.004339922782758488 0.0 32-33 0.0 0.0 0.0 0.007720054950099234 0.0 34-35 8.346005351458631E-5 0.0 0.0 0.01030731660905141 0.0 36-37 8.346005351458631E-5 0.0 0.0 0.012059977732857722 0.0 38-39 8.346005351458631E-5 0.0 0.0 0.01414647907072238 0.0 40-41 8.346005351458631E-5 0.0 0.0 0.016441630542373506 0.0 42-43 8.346005351458631E-5 0.0 0.0 0.018945432147811095 0.0 44-45 8.346005351458631E-5 0.0 0.0 0.022242104261637252 0.0 46-47 8.346005351458631E-5 0.0 0.0 0.026248186830337396 0.0 48-49 8.346005351458631E-5 0.0 0.0 0.031088869934183402 0.0 50-51 8.346005351458631E-5 0.0 0.0 0.038057784402651354 0.0 52-53 8.346005351458631E-5 0.0 0.0 0.056502456229374935 0.0 54-55 8.346005351458631E-5 0.0 0.0 0.07678324923341942 0.0 56-57 8.346005351458631E-5 0.0 0.0 0.09994341408371711 0.0 58-59 8.346005351458631E-5 0.0 0.0 0.12882059259976397 0.0 60-61 8.346005351458631E-5 0.0 0.0 0.17238674053437802 0.0 62-63 8.346005351458631E-5 0.0 0.0 0.20447713111073645 0.0 64-65 8.346005351458631E-5 0.0 0.0 0.23640060158006573 0.0 66-67 8.346005351458631E-5 0.0 0.0 0.29115039668563436 0.0 68-69 8.346005351458631E-5 0.0 0.0 0.36154895182518787 0.0 70-71 8.346005351458631E-5 0.0 0.0 0.4217236504092047 0.0 72-73 8.346005351458631E-5 0.0 0.0 0.48527848116056216 0.0 74-75 8.346005351458631E-5 0.0 0.0 0.5919821595789607 0.0 76-77 8.346005351458631E-5 0.0 0.0 0.6721038109529636 0.0 78-79 8.346005351458631E-5 0.0 0.0 0.7482611097850236 0.0 80-81 1.6692010702917261E-4 0.0 0.0 0.8404010088651268 0.0 82-83 1.6692010702917261E-4 0.0 0.0 0.9849120915256331 0.0 84-85 1.6692010702917261E-4 0.0 0.0 1.1229132900120016 0.0 86-87 1.6692010702917261E-4 0.0 0.0 1.3168744543799002 0.0 88-89 1.6692010702917261E-4 0.0 0.0 1.5592424497862587 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7855 0.0 27.287025 1 CTCGCGA 35 0.0083868485 27.147173 36-37 GTATCAA 10585 0.0 25.457634 1 GTATAGA 325 0.0 24.859444 1 GAGTACT 6615 0.0 23.481522 12-13 GTACATG 7460 0.0 22.297443 1 GTACTTT 6970 0.0 22.21554 14-15 TACATGG 7735 0.0 21.254505 2 AGTACTT 6920 0.0 21.210976 12-13 GTATAGG 405 0.0 21.122404 1 ATCAACG 12735 0.0 21.117994 3 TATCAAC 12860 0.0 21.06052 2 TCAACGC 12785 0.0 21.035406 4 CAACGCA 12920 0.0 20.815609 5 ACATGGG 7630 0.0 20.737427 3 AACGCAG 13145 0.0 20.523897 6 TACCTGG 840 0.0 20.36378 2 GTACCTG 820 0.0 20.285234 1 CGATCGT 200 0.0 20.186499 24-25 TAGACTG 450 0.0 20.062096 5 >>END_MODULE