FastQCFastQC Report
Thu 26 May 2016
SRR937757_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937757_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157798
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79550.6870801296944717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61570.5317853373386376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42470.36681700953015983No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17290.1493352035501875No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16770.14484391923288864No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14880.12851982815655236No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14750.12739700707722765No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12000.10364502270689706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84650.030.8960251
GGTATCA58800.030.083781
GTACATG68400.028.5727841
TACATGG70950.026.6687952
ACATGGG71500.025.3975853
ATCAACG102050.025.340843
TATCAAC102350.025.31522
TCAACGC102700.025.2267444
CATGGGG26950.024.8711264
CAACGCA103750.024.8339775
AACGCAG106000.024.3796376
TAGGGCG1000.001646099423.768685
GAGTACT55950.023.55812312-13
GTATAGG3900.023.1662541
GTACTTT57450.022.48684114-15
ACGCAGA117000.021.8845257
CGCAGAG117150.021.7754028
CATGGGA43750.021.6227114
AGTACTT58200.021.34157612-13
GGACCGA2457.510607E-921.3285246
Produced by FastQC (version 0.11.5)