##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937757_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1157798 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.552614532068635 33.0 31.0 34.0 30.0 34.0 2 31.679927759419172 33.0 31.0 34.0 30.0 34.0 3 31.68687283964906 33.0 31.0 34.0 30.0 34.0 4 35.30847781737401 37.0 35.0 37.0 33.0 37.0 5 35.29042026329291 37.0 35.0 37.0 33.0 37.0 6 35.34585825852178 37.0 35.0 37.0 33.0 37.0 7 35.27803295566239 37.0 35.0 37.0 33.0 37.0 8 35.26463079051786 37.0 35.0 37.0 33.0 37.0 9 36.84675219684263 39.0 37.0 39.0 33.0 39.0 10-11 36.720991917415645 39.0 37.0 39.0 32.0 39.0 12-13 36.57557017718116 39.0 37.0 39.0 32.0 39.0 14-15 37.6600261876424 40.0 37.0 41.0 32.0 41.0 16-17 37.5866446478574 40.0 37.0 41.0 32.0 41.0 18-19 37.544739669614216 40.0 37.0 41.0 32.0 41.0 20-21 37.470731941150355 40.0 37.0 41.0 32.0 41.0 22-23 37.257602362415554 39.5 37.0 41.0 31.5 41.0 24-25 37.06615704984807 39.0 36.0 41.0 31.0 41.0 26-27 36.99896916387833 39.0 36.0 41.0 31.0 41.0 28-29 37.03492405410961 39.0 36.0 41.0 31.0 41.0 30-31 36.830181948837364 39.0 36.0 41.0 30.5 41.0 32-33 36.568210948714714 39.0 35.5 40.5 30.0 41.0 34-35 36.568167331434324 39.0 35.0 41.0 30.0 41.0 36-37 36.453329941837865 39.0 35.0 41.0 30.0 41.0 38-39 36.30248540764451 39.0 35.0 41.0 30.0 41.0 40-41 36.05057531624688 39.0 35.0 40.5 29.5 41.0 42-43 35.76471629766159 39.0 35.0 40.0 28.5 41.0 44-45 35.48230174866427 38.5 35.0 40.0 27.0 41.0 46-47 35.07570750683625 38.0 34.5 40.0 25.5 41.0 48-49 34.67208053563748 38.0 34.0 40.0 24.0 41.0 50-51 33.7144368015837 37.5 33.0 39.5 22.5 40.5 52-53 33.80923615345682 38.0 33.0 39.5 23.0 40.5 54-55 34.14038372842241 38.0 34.0 40.0 23.0 41.0 56-57 33.914143486169436 38.0 33.5 40.0 21.5 41.0 58-59 33.637881996686815 38.0 33.0 40.0 20.5 41.0 60-61 33.29488736377157 37.0 33.0 40.0 18.5 41.0 62-63 32.91744371643413 36.5 32.0 40.0 17.5 41.0 64-65 32.52676503155127 36.0 32.0 39.0 14.0 41.0 66-67 32.08657511932133 35.5 31.0 39.0 10.0 40.5 68-69 31.581357024282305 35.0 31.0 38.5 7.5 40.0 70-71 31.114853368204123 35.0 30.5 38.0 6.5 40.0 72-73 30.637479508515305 35.0 30.0 37.0 2.0 39.5 74-75 30.160908465898196 34.5 29.5 37.0 2.0 39.0 76-77 29.652027383015 34.0 29.0 36.0 2.0 39.0 78-79 29.099189582293285 34.0 28.5 36.0 2.0 37.5 80-81 28.54806322000902 34.0 27.5 35.0 2.0 37.0 82-83 27.9906728116649 34.0 26.5 35.0 2.0 36.5 84-85 27.492262035346407 33.0 26.0 35.0 2.0 36.0 86-87 26.996432020093316 33.0 25.5 35.0 2.0 36.0 88-89 26.47087488491084 32.5 24.5 35.0 2.0 35.0 90-91 25.927355635438996 32.0 23.5 35.0 2.0 35.0 92-93 25.301730094541533 32.0 20.0 34.0 2.0 35.0 94-95 24.609113593217472 31.5 16.5 34.0 2.0 35.0 96-97 23.814958222418767 31.0 4.5 34.0 2.0 35.0 98-99 22.818055913034918 31.0 2.0 34.0 2.0 35.0 100-101 20.471940269373412 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14036313196036332 1101 2 0.2118782744322587 1101 3 0.12090259836280026 1101 4 0.24456230379791322 1101 5 0.16562438323646234 1101 6 0.22266413235985283 1101 7 0.24146799291215615 1101 8 0.05873468760100309 1101 9 0.3453433100846368 1101 10-11 0.17034722254896195 1101 12-13 0.44261363042089386 1101 14-15 0.5793032440084431 1101 16-17 0.6707893438725776 1101 18-19 0.6145633556592074 1101 20-21 0.6127629066307207 1101 22-23 0.5027840920469231 1101 24-25 0.5321435249005546 1101 26-27 0.6449157194619772 1101 28-29 0.4354119625857038 1101 30-31 0.48091859336376785 1101 32-33 0.5100984895163236 1101 34-35 0.7703660786540283 1101 36-37 0.6025637438889291 1101 38-39 0.6963811125373951 1101 40-41 0.6876864516392445 1101 42-43 0.792981837951352 1101 44-45 0.8343666977697097 1101 46-47 0.9906767440506883 1101 48-49 1.0877754055752789 1101 50-51 0.8948985861178045 1101 52-53 1.104597391268129 1101 54-55 1.1587417029720442 1101 56-57 1.2374637762695784 1101 58-59 1.2066283854567033 1101 60-61 1.1028268200223792 1101 62-63 1.169650104965605 1101 64-65 0.8898047699949174 1101 66-67 1.1754775498841177 1101 68-69 1.0762698372290167 1101 70-71 1.2425752849184697 1101 72-73 1.3525716014921265 1101 74-75 1.1678402354082955 1101 76-77 1.3880546829293188 1101 78-79 1.623115098189512 1101 80-81 1.4830051953463652 1101 82-83 1.555552037821382 1101 84-85 1.738267835340885 1101 86-87 1.9867093248283219 1101 88-89 1.9198028421919027 1101 90-91 1.9545880091661694 1101 92-93 1.951097822271123 1101 94-95 1.9639681078543916 1101 96-97 2.3260958833720444 1101 98-99 2.6922342532096692 1101 100-101 3.1214122844006944 1102 1 0.2562505385111038 1102 2 0.14140030552295357 1102 3 0.2508163609196217 1102 4 0.1375116808753205 1102 5 0.03921979115239793 1102 6 0.1271969784563396 1102 7 0.08986715466768658 1102 8 0.23569535576184109 1102 9 0.26872089617534556 1102 10-11 0.2972219141310788 1102 12-13 0.20946357248724468 1102 14-15 0.2777781651512825 1102 16-17 0.24277353331235219 1102 18-19 0.37384061979969374 1102 20-21 0.4907886626485407 1102 22-23 0.4838102784910916 1102 24-25 0.5441052473885932 1102 26-27 0.34960682333168336 1102 28-29 0.2665829288059314 1102 30-31 0.3992446478540046 1102 32-33 0.361845411859143 1102 34-35 0.4751862560853439 1102 36-37 0.540055727741418 1102 38-39 0.5230964594110361 1102 40-41 0.5421409297038053 1102 42-43 0.49899383989044566 1102 44-45 0.462167186455801 1102 46-47 0.5294640286472969 1102 48-49 0.6058296719048499 1102 50-51 0.3743340428153914 1102 52-53 0.3692900385859019 1102 54-55 0.6639657855443417 1102 56-57 0.7180758403813741 1102 58-59 0.5264821817954726 1102 60-61 0.6930410875693198 1102 62-63 0.7484216616090649 1102 64-65 0.6942662044451211 1102 66-67 0.9380210544677219 1102 68-69 1.0900562522774173 1102 70-71 1.1030448626876002 1102 72-73 1.2158666674582292 1102 74-75 1.3416327590328336 1102 76-77 1.1494881548831266 1102 78-79 1.3551238741047662 1102 80-81 1.4572565143422977 1102 82-83 1.405218566084958 1102 84-85 1.329982345274388 1102 86-87 1.3334030851677916 1102 88-89 1.525551588412089 1102 90-91 1.4905707290694785 1102 92-93 1.4949790289431668 1102 94-95 1.7031710364927442 1102 96-97 1.8962814666975838 1102 98-99 1.9501365442518015 1102 100-101 2.5726991070498144 1103 1 0.19521858044072005 1103 2 0.18445055019207857 1103 3 0.12460904758444613 1103 4 0.2964525929009554 1103 5 0.12485344179835778 1103 6 0.15224159714858132 1103 7 0.11915516859784958 1103 8 0.1098836868597104 1103 9 0.15186666071473098 1103 10-11 0.16219303426134246 1103 12-13 0.22838086540978253 1103 14-15 0.30650858129531855 1103 16-17 0.32090053734922463 1103 18-19 0.22279167293125823 1103 20-21 0.17799641293169088 1103 22-23 0.3823244923434288 1103 24-25 0.4405942291259066 1103 26-27 0.1582589366598981 1103 28-29 0.19931114716688114 1103 30-31 0.14585558372699836 1103 32-33 0.13315260367495796 1103 34-35 0.1321155226866466 1103 36-37 0.2941871686480084 1103 38-39 0.41988000060261754 1103 40-41 0.4304588756570382 1103 42-43 0.6080300217912367 1103 44-45 0.4185030951010589 1103 46-47 0.6367108433835256 1103 48-49 0.4539725886738708 1103 50-51 0.5383381709954875 1103 52-53 0.33069079378851995 1103 54-55 0.3696386636836806 1103 56-57 0.304179862259204 1103 58-59 0.462373381750254 1103 60-61 0.574287161015711 1103 62-63 0.5380711575971944 1103 64-65 0.48098342084740153 1103 66-67 0.45976220518433664 1103 68-69 0.44580282462709064 1103 70-71 0.6525085688324772 1103 72-73 0.6172120759176316 1103 74-75 0.4358165141332755 1103 76-77 0.4970242900457009 1103 78-79 0.7280076111620843 1103 80-81 0.48485841995718815 1103 82-83 0.6511784277398398 1103 84-85 0.9172893327463711 1103 86-87 0.8888590653768738 1103 88-89 0.9294396101681848 1103 90-91 1.1369453108711376 1103 92-93 1.1167761024786849 1103 94-95 1.0592238528506819 1103 96-97 1.3846577810740435 1103 98-99 1.3000919255595598 1103 100-101 1.8523240709092157 1104 1 0.08626302109106376 1104 2 0.13306219860025337 1104 3 0.14853401522055165 1104 4 0.1860398479024994 1104 5 0.22842492439966122 1104 6 0.16683344468925299 1104 7 0.1992414867214123 1104 8 0.21755927854083978 1104 9 0.16614999768607674 1104 10-11 0.215649109055974 1104 12-13 0.33399440702466876 1104 14-15 0.532998833225804 1104 16-17 0.5125671860059313 1104 18-19 0.5571069091390584 1104 20-21 0.5006357937925756 1104 22-23 0.5212366440938894 1104 24-25 0.47542647717920516 1104 26-27 0.4421008171587886 1104 28-29 0.31485781060616347 1104 30-31 0.36958355844966206 1104 32-33 0.38794127123347266 1104 34-35 0.5624029399939232 1104 36-37 0.5233848574662048 1104 38-39 0.5854099688114474 1104 40-41 0.6564398960435867 1104 42-43 0.7235702016458703 1104 44-45 0.7710458489388046 1104 46-47 0.6707950478584976 1104 48-49 0.6492622103999324 1104 50-51 0.6164170092235679 1104 52-53 0.627845094458273 1104 54-55 0.7459083656765131 1104 56-57 0.7994541395016057 1104 58-59 0.783375387339099 1104 60-61 0.9061651096959693 1104 62-63 0.7136841805253127 1104 64-65 0.49313635996438876 1104 66-67 0.5587387173183664 1104 68-69 0.759700712491334 1104 70-71 0.7791229814089036 1104 72-73 0.7846800956363715 1104 74-75 0.7416118122376965 1104 76-77 0.9840309162692087 1104 78-79 1.064542664662465 1104 80-81 0.9160330077632928 1104 82-83 1.1025266891666163 1104 84-85 0.9281982340597281 1104 86-87 1.2599589190639833 1104 88-89 1.4882940796272592 1104 90-91 1.2931375664453988 1104 92-93 1.3827373371260983 1104 94-95 1.496172883230603 1104 96-97 1.7270367055337985 1104 98-99 1.9327303987186006 1104 100-101 2.135492469053183 1105 1 -0.009359255818424117 1105 2 0.03426203583708798 1105 3 0.07678457093122404 1105 4 -0.033782952359828755 1105 5 0.048219370972432785 1105 6 0.04901574809104403 1105 7 -0.04283950093932276 1105 8 0.1579304954335825 1105 9 0.07313474513262008 1105 10-11 0.11112355718712053 1105 12-13 0.05174668451854103 1105 14-15 0.2570592655438162 1105 16-17 0.3452079101288703 1105 18-19 0.2684208970313833 1105 20-21 0.04232221064376063 1105 22-23 0.22435105880664707 1105 24-25 0.2364181312382243 1105 26-27 0.33663077358934146 1105 28-29 0.37632092591910293 1105 30-31 0.2668035982314052 1105 32-33 0.2031910109639199 1105 34-35 0.28525103564325605 1105 36-37 0.30447029514111534 1105 38-39 0.3317545613732591 1105 40-41 0.35534535705077275 1105 42-43 0.5839493835445353 1105 44-45 0.4378737636872643 1105 46-47 0.47578876026550887 1105 48-49 0.47034150116810025 1105 50-51 0.23603197261267894 1105 52-53 0.38459830481018287 1105 54-55 0.4392786345603952 1105 56-57 0.649584057838652 1105 58-59 0.7010574299302377 1105 60-61 0.668968640271423 1105 62-63 0.7620832251574754 1105 64-65 0.5873761239577888 1105 66-67 0.5264937003169727 1105 68-69 0.6836635843088423 1105 70-71 0.9200134928791606 1105 72-73 0.8249512629642624 1105 74-75 0.691195543280223 1105 76-77 0.7131133064635939 1105 78-79 0.9450938192636578 1105 80-81 1.197419568114448 1105 82-83 1.1601355424438502 1105 84-85 1.2399408280110862 1105 86-87 1.4594249606508392 1105 88-89 1.397581482147924 1105 90-91 1.7233501541849208 1105 92-93 1.8158239825383156 1105 94-95 1.7942553838095812 1105 96-97 1.8290918610353906 1105 98-99 1.9174231940677302 1105 100-101 2.379065813121958 1106 1 0.07228801458947132 1106 2 0.12671230787266552 1106 3 0.11764915960158007 1106 4 0.008214391764305162 1106 5 0.06184380503038511 1106 6 -0.028591942098600498 1106 7 0.1059766824764452 1106 8 -0.09705972801550189 1106 9 0.09956454392040115 1106 10-11 0.0023215245010277386 1106 12-13 -0.05184728713120279 1106 14-15 0.0014678103538798837 1106 16-17 -0.04620266667021866 1106 18-19 0.17969390503734672 1106 20-21 0.20363268671473378 1106 22-23 0.02195467107664939 1106 24-25 -0.024503374036932257 1106 26-27 0.009568548718213776 1106 28-29 0.20192625300659728 1106 30-31 0.233533699203484 1106 32-33 0.17433537280435019 1106 34-35 0.07320357144704559 1106 36-37 0.2096595350099193 1106 38-39 0.23597834505530102 1106 40-41 0.24280958811894493 1106 42-43 0.2977796305549276 1106 44-45 0.2663327631679451 1106 46-47 0.34206872164726576 1106 48-49 0.29766338844324736 1106 50-51 0.09109329825024304 1106 52-53 0.06506193290391593 1106 54-55 -0.044227574682189186 1106 56-57 0.02265445249064868 1106 58-59 0.3103883722782186 1106 60-61 0.2147615865160759 1106 62-63 0.4433301972150119 1106 64-65 0.4593006929007757 1106 66-67 0.4765853663736941 1106 68-69 0.28726975486347683 1106 70-71 0.4867685144712226 1106 72-73 0.5972796156590015 1106 74-75 0.8558807592531217 1106 76-77 0.8253127341606827 1106 78-79 1.0963462982554013 1106 80-81 1.0205432894078115 1106 82-83 0.6498605791276972 1106 84-85 0.84311916554433 1106 86-87 1.1721965169871211 1106 88-89 1.3162487692160383 1106 90-91 1.4174617427960214 1106 92-93 1.2602980528946297 1106 94-95 1.3852060618944186 1106 96-97 1.4487587612239459 1106 98-99 1.4011132582339663 1106 100-101 1.9043584997042124 1107 1 0.11426505001216825 1107 2 0.19861666189002136 1107 3 0.1496365677559197 1107 4 0.19508077869249973 1107 5 0.2405945233510849 1107 6 0.06977010599184297 1107 7 0.23911629510732268 1107 8 0.2569016073562338 1107 9 0.21014436877664622 1107 10-11 0.28246659817633457 1107 12-13 0.10354942467672146 1107 14-15 0.2674741641037883 1107 16-17 0.4140882035188511 1107 18-19 0.4491689797607705 1107 20-21 0.4233168424575098 1107 22-23 0.4337060363377532 1107 24-25 0.38814611798834875 1107 26-27 0.3212085968592504 1107 28-29 0.3739102999621764 1107 30-31 0.33517381962837334 1107 32-33 0.4722929927864996 1107 34-35 0.4256785702724315 1107 36-37 0.4510649859346145 1107 38-39 0.4359506964848947 1107 40-41 0.4151026485084728 1107 42-43 0.6065554313695714 1107 44-45 0.650841251098349 1107 46-47 0.4880956401508456 1107 48-49 0.46838608231755785 1107 50-51 0.6656197487159332 1107 52-53 0.5299482484879476 1107 54-55 0.6572645445716958 1107 56-57 0.7037074112491055 1107 58-59 0.6823004968088284 1107 60-61 0.7886049546540832 1107 62-63 0.8857281053205597 1107 64-65 0.7440891000178382 1107 66-67 0.814791534354125 1107 68-69 0.7894988079089806 1107 70-71 1.0937928777409134 1107 72-73 1.148864981071906 1107 74-75 1.1824591673460212 1107 76-77 1.4764681914102091 1107 78-79 1.2271439143342207 1107 80-81 1.0814686310393284 1107 82-83 1.291771836252174 1107 84-85 1.5175888731923202 1107 86-87 1.6993336791956857 1107 88-89 1.812948108180283 1107 90-91 1.6551771034743332 1107 92-93 1.7483676044095198 1107 94-95 1.8461267050760668 1107 96-97 1.923008545275966 1107 98-99 2.3286758108656436 1107 100-101 2.709543600558245 1108 1 0.2904615836329647 1108 2 0.2202957933368488 1108 3 0.1586874272347103 1108 4 0.16998537416495196 1108 5 0.21994750745886193 1108 6 0.1989151085236145 1108 7 0.2007927565800074 1108 8 -0.02657084103945806 1108 9 0.2580257090095657 1108 10-11 0.3040481050179906 1108 12-13 0.3926094114680012 1108 14-15 0.36520639157382107 1108 16-17 0.43269627660321675 1108 18-19 0.39525255700859674 1108 20-21 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0.3844137874294731 2310 36-37 0.3467536702746443 2310 38-39 0.4104319568564634 2310 40-41 0.35421014540430207 2310 42-43 0.31636382348406755 2310 44-45 0.5142173394025065 2310 46-47 0.31182799735114486 2310 48-49 0.4350966159897851 2310 50-51 0.6804694059204763 2310 52-53 0.05490789882982483 2310 54-55 0.5565037530732937 2310 56-57 0.7742147141225217 2310 58-59 0.9822104281391475 2310 60-61 1.0481305151718132 2310 62-63 0.7002612934135541 2310 64-65 0.6034308368932244 2310 66-67 0.5731632820365142 2310 68-69 0.8313776673914042 2310 70-71 0.851469757310003 2310 72-73 0.9357155696823334 2310 74-75 1.0429885081674648 2310 76-77 0.9702497054608621 2310 78-79 1.1573179631298132 2310 80-81 1.3139514728348765 2310 82-83 1.1780189770634202 2310 84-85 1.3022494906357842 2310 86-87 1.305466028531871 2310 88-89 1.701331768765165 2310 90-91 1.8568906814184452 2310 92-93 1.7685236947657224 2310 94-95 2.0230695557064564 2310 96-97 2.162654488161614 2310 98-99 2.3684642293617912 2310 100-101 2.5327024304519483 2311 1 0.26103970280093236 2311 2 0.12271633814256688 2311 3 0.11521599133780924 2311 4 0.16671333060281768 2311 5 0.04474945812704334 2311 6 0.17256783237778706 2311 7 0.1380902160370141 2311 8 0.35492432240574345 2311 9 0.24265198791465536 2311 10-11 0.1590840172658332 2311 12-13 0.08351082807449473 2311 14-15 0.37021178102723695 2311 16-17 0.13116350907527874 2311 18-19 0.338084583966328 2311 20-21 0.3906447132116142 2311 22-23 0.386107778457621 2311 24-25 0.43310483227858754 2311 26-27 0.4508541619409243 2311 28-29 0.32249456393762443 2311 30-31 0.4339431574751842 2311 32-33 0.4823766187580034 2311 34-35 0.4177219756316646 2311 36-37 0.40617270841872255 2311 38-39 0.2714500795719914 2311 40-41 0.027176214054158265 2311 42-43 0.1509974151927409 2311 44-45 0.1940473000084708 2311 46-47 0.3693319968846822 2311 48-49 0.2984597955005128 2311 50-51 0.09795547588224451 2311 52-53 0.09133371887085673 2311 54-55 0.17214110926318682 2311 56-57 0.18632628562032494 2311 58-59 0.23172629325920013 2311 60-61 0.19900494621766285 2311 62-63 0.08740484295857698 2311 64-65 0.31111431435552817 2311 66-67 0.33664817855882845 2311 68-69 0.46142136161203595 2311 70-71 0.4317778398297669 2311 72-73 0.4882029333428797 2311 74-75 0.5595015708808795 2311 76-77 0.47477036273272333 2311 78-79 0.6030948565764085 2311 80-81 0.7169342684632696 2311 82-83 0.6162785695665285 2311 84-85 0.8612789614583214 2311 86-87 0.9793952650309414 2311 88-89 0.9395417998503781 2311 90-91 0.9878038869853576 2311 92-93 1.129033448525643 2311 94-95 1.2880904917612916 2311 96-97 1.2745149727702447 2311 98-99 1.340952904942224 2311 100-101 1.7031762157416814 2312 1 0.11023209699434844 2312 2 0.14835526678701427 2312 3 0.09359805362909412 2312 4 0.009594813598795326 2312 5 0.04337277987447408 2312 6 0.0678857632712706 2312 7 -0.07743489811823423 2312 8 0.11055711733520468 2312 9 0.0016924022811792838 2312 10-11 -0.0880365443098512 2312 12-13 0.18677532749519798 2312 14-15 0.11186652102587402 2312 16-17 0.0523725324515496 2312 18-19 0.17310128130156954 2312 20-21 0.23765623096531385 2312 22-23 0.20280511369921328 2312 24-25 0.1342906838426856 2312 26-27 0.07507552089955993 2312 28-29 0.09914555555488391 2312 30-31 -0.03203517006519263 2312 32-33 -0.04418236713445367 2312 34-35 -0.17181663269655445 2312 36-37 -0.23224479311471669 2312 38-39 -0.0321142903591749 2312 40-41 -0.023372412675207954 2312 42-43 0.31191202024965037 2312 44-45 -0.010478018603947703 2312 46-47 0.2873855646622303 2312 48-49 0.36035579495528935 2312 50-51 0.42738367734071403 2312 52-53 0.270499533632659 2312 54-55 0.43776504438211106 2312 56-57 0.651568567362304 2312 58-59 0.7646370817823396 2312 60-61 0.6187486932359931 2312 62-63 0.8868391781320284 2312 64-65 0.8411695177904122 2312 66-67 1.1151052084777255 2312 68-69 0.9254369003566687 2312 70-71 0.9078427209038473 2312 72-73 0.8309890029946239 2312 74-75 0.7892841647607938 2312 76-77 0.923260787322107 2312 78-79 1.0816404846006726 2312 80-81 0.8485569768391983 2312 82-83 0.7115965004600042 2312 84-85 0.8219550331546372 2312 86-87 0.7032758620458672 2312 88-89 0.9707147024606151 2312 90-91 1.0659201549605513 2312 92-93 1.0438497778379876 2312 94-95 1.1607613838760606 2312 96-97 1.103329802730034 2312 98-99 1.4859402967503073 2312 100-101 1.9320591980902115 2313 1 0.3487232511819158 2313 2 0.29907056993930325 2313 3 0.35099158390303486 2313 4 0.1478516621361905 2313 5 0.18810761193822145 2313 6 0.2505770784794592 2313 7 0.18107132594815312 2313 8 0.20056558123975066 2313 9 0.3365254982123105 2313 10-11 0.22034891428926784 2313 12-13 0.14067182013572932 2313 14-15 -0.08906605674915369 2313 16-17 0.016793683955441452 2313 18-19 0.14659222905243752 2313 20-21 0.10488285717038792 2313 22-23 0.19755646069866373 2313 24-25 0.2638808037824063 2313 26-27 0.31165249082173574 2313 28-29 0.377416028914233 2313 30-31 -0.29810936341380767 2313 32-33 -0.14922922826448826 2313 34-35 0.2380861356195325 2313 36-37 0.3975471186955275 2313 38-39 0.44352514730726966 2313 40-41 0.3150568105034992 2313 42-43 0.5457382211951227 2313 44-45 0.36665240810070543 2313 46-47 0.5540408773106122 2313 48-49 0.5150909366740777 2313 50-51 0.7587826988307711 2313 52-53 0.7438991331454758 2313 54-55 0.8104792740635816 2313 56-57 0.6194748863895754 2313 58-59 0.5333557292091058 2313 60-61 0.5345266499255814 2313 62-63 0.7390764358269521 2313 64-65 0.6216597017255125 2313 66-67 0.6208720175857216 2313 68-69 0.7522865731545032 2313 70-71 0.8131496922710788 2313 72-73 0.7914878572709583 2313 74-75 0.6273557363633593 2313 76-77 0.6016318985708402 2313 78-79 0.6630813095217114 2313 80-81 0.6065641401925532 2313 82-83 0.7005118820582368 2313 84-85 0.9138931640767893 2313 86-87 0.7724796357086738 2313 88-89 0.7702249313989107 2313 90-91 0.5008559531480365 2313 92-93 0.559584898913112 2313 94-95 1.047645428383813 2313 96-97 1.1620393669421674 2313 98-99 1.3521713819880183 2313 100-101 1.7841880765196443 2314 1 0.046652677870596904 2314 2 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2314 62-63 0.37831238386532107 2314 64-65 0.5690801397099889 2314 66-67 0.7687256709262833 2314 68-69 0.7917970482751819 2314 70-71 0.5550367391440361 2314 72-73 0.608895270954708 2314 74-75 0.9193706053698811 2314 76-77 1.1361009041243264 2314 78-79 1.0688711922061707 2314 80-81 0.8993125339428261 2314 82-83 1.0625523397002112 2314 84-85 1.268450095764571 2314 86-87 1.3939421339535407 2314 88-89 1.511034689456789 2314 90-91 1.357227329705264 2314 92-93 1.2800242148710268 2314 94-95 1.6555110892581375 2314 96-97 1.5247576692318283 2314 98-99 1.5114382445840668 2314 100-101 1.486159757138612 2315 1 0.12008067427144908 2315 2 0.042137039124238385 2315 3 0.05918971385656491 2315 4 0.1310732524671252 2315 5 0.15615627384874387 2315 6 0.1203550245940832 2315 7 0.22937709098258097 2315 8 0.19722459737370457 2315 9 0.14647665540249477 2315 10-11 0.18418884102505473 2315 12-13 0.20035728148545928 2315 14-15 0.2679796881602243 2315 16-17 0.11541630541236003 2315 18-19 0.13864820607822992 2315 20-21 0.004162934504833515 2315 22-23 0.13982979953435404 2315 24-25 0.326493159204297 2315 26-27 0.18490850854986718 2315 28-29 0.20870199678199697 2315 30-31 0.14445228367427632 2315 32-33 0.17754415014292135 2315 34-35 0.28417588428258256 2315 36-37 0.24452327390712014 2315 38-39 0.45219983384270535 2315 40-41 0.4117842291836169 2315 42-43 0.2336968291750452 2315 44-45 0.4227665216376266 2315 46-47 0.06950494053491241 2315 48-49 0.16588604560937625 2315 50-51 0.20082708699543161 2315 52-53 0.034881303258650576 2315 54-55 0.07883489946636502 2315 56-57 0.06682471170869064 2315 58-59 0.206623335798362 2315 60-61 0.06448526921170838 2315 62-63 0.17479924157502325 2315 64-65 0.19811367981407813 2315 66-67 0.24225827150049284 2315 68-69 0.35693540239687493 2315 70-71 0.3775879999581697 2315 72-73 0.20827559455995726 2315 74-75 0.5286398387071394 2315 76-77 0.5114804519417859 2315 78-79 0.5803919588371222 2315 80-81 0.5939987055254647 2315 82-83 0.40943360919426297 2315 84-85 0.2436598605164626 2315 86-87 0.30346429318716517 2315 88-89 0.3450168972089429 2315 90-91 0.6272070447622262 2315 92-93 0.7249226152856281 2315 94-95 0.6108555994467118 2315 96-97 1.0215201859637268 2315 98-99 0.9607250921195103 2315 100-101 1.2920331701915622 2316 1 -0.14608127286521366 2316 2 -0.2810252567556084 2316 3 -0.10864390115395395 2316 4 -0.10706660504637 2316 5 -0.2578804476587493 2316 6 -0.08323626372455806 2316 7 -0.03411562948251401 2316 8 -0.1329048974852043 2316 9 -0.23850324396018863 2316 10-11 0.04095982405927856 2316 12-13 -0.035994788171194614 2316 14-15 -0.34050243587737583 2316 16-17 0.045524914003699735 2316 18-19 -0.24477767833064945 2316 20-21 -0.2386772912967956 2316 22-23 0.11498472198689313 2316 24-25 0.3034071269436325 2316 26-27 0.17391255933606686 2316 28-29 -0.24602941348178575 2316 30-31 -0.017273535201546508 2316 32-33 0.21896602592136105 2316 34-35 -0.2078873340421481 2316 36-37 -0.1508374836703723 2316 38-39 -0.4111809822163437 2316 40-41 -0.1901503373975899 2316 42-43 -0.28477123016494943 2316 44-45 -0.26582785171686396 2316 46-47 -0.23402426383242414 2316 48-49 -0.3721777399020638 2316 50-51 -0.09366928569222921 2316 52-53 -0.10224854034345299 2316 54-55 -0.5363978961892926 2316 56-57 -0.23131238651808417 2316 58-59 -0.1306438972885502 2316 60-61 -0.12406886454471788 2316 62-63 0.06045842367645804 2316 64-65 0.1669184104578747 2316 66-67 0.29247154137022946 2316 68-69 0.23638427319710686 2316 70-71 0.20805474097228327 2316 72-73 0.2952352821473845 2316 74-75 0.09345529224573212 2316 76-77 0.07077637394595016 2316 78-79 -0.2313116324511988 2316 80-81 0.09813188709802745 2316 82-83 0.03768966333760204 2316 84-85 0.1614369484725593 2316 86-87 0.1992264886596331 2316 88-89 -0.06382987423397068 2316 90-91 0.09483045577714577 2316 92-93 0.04290933238592487 2316 94-95 0.6329279770704268 2316 96-97 0.7766593574878904 2316 98-99 0.7381014320023169 2316 100-101 1.0263598467456703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5371.0 3 1100.0 4 1097.0 5 1285.0 6 1451.0 7 1742.0 8 2062.0 9 2496.0 10 3233.0 11 4014.0 12 5113.0 13 6441.0 14 7190.0 15 9004.0 16 11449.0 17 14579.0 18 12895.0 19 9281.0 20 9239.0 21 9714.0 22 10781.0 23 11797.0 24 13193.0 25 14929.0 26 17146.0 27 19927.0 28 23651.0 29 27908.0 30 33836.0 31 40935.0 32 50908.0 33 65014.0 34 86050.0 35 118875.0 36 158321.0 37 196825.0 38 135002.0 39 13944.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.844090564402556 19.026497670891562 14.845455530278409 22.283956234427475 2 15.053648840330688 20.18380024244441 41.01157719705497 23.750973720169934 3 18.91419853571184 25.45627971005327 29.75445320506878 25.8750685491661 4 12.35024630157846 16.725120162428247 37.134066535640756 33.79056700035254 5 13.228643814555769 37.98705095169009 33.604815833351374 15.179489400402762 6 31.047381438194115 36.32640411649672 17.51294412794105 15.113270317368116 7 28.340548115716974 33.538826955154086 20.272716177299273 17.847908751829664 8 26.529085743077236 34.717107233820045 19.320631424757295 19.43317559834543 9 27.033674461448427 16.96674455388286 20.39626383767831 35.603317146990406 10-11 25.08162850987442 26.969592162624163 26.51356334039056 21.435215987110855 12-13 26.083885209713024 24.50742327836212 27.452408778080766 21.95628273384409 14-15 23.045555868158505 25.569589023308158 26.38158720540178 25.003267903131558 16-17 21.5717081793542 29.124108695633353 26.926589501804465 22.37759362320798 18-19 21.507895182209662 27.21642291392626 29.996887894515638 21.278794009348438 20-21 22.41177549967629 26.120181971146355 30.043415490397695 21.424627038779658 22-23 22.12374215782409 26.007117567079867 30.171205875454692 21.69793439964135 24-25 21.874886405926798 26.342876677946737 30.17075893823079 21.611477977895674 26-27 21.650044946378127 26.48170485485804 30.246120913331175 21.622129285432656 28-29 21.302487741803663 26.55709503581467 30.50023657117676 21.64018065120491 30-31 21.469770010808407 26.388438598777007 30.373004697782353 21.768786692632233 32-33 21.283524638845552 26.616429129229484 30.254379479258837 21.845666752666126 34-35 21.853616392830972 26.52970769938628 29.920959240834737 21.695716666948012 36-37 21.826135086514416 26.23522625132477 29.93593913027297 22.002699531887842 38-39 21.576482461120882 26.67317825539045 30.02477115674897 21.725568126739695 40-41 21.508654879304235 26.608761201838423 30.225934303319107 21.656649615538235 42-43 21.481920374078836 26.591356351727246 29.696792379580607 22.22993089461331 44-45 21.514797549920605 26.692284621566774 29.575061920636852 22.21785590787577 46-47 21.809648080705788 27.052451403630634 29.035646353892798 22.102254161770784 48-49 21.89360663315922 27.08186529035654 28.786858338974657 22.237669737509588 50-51 21.85260090334456 27.248599833252673 28.626256557126545 22.272542706276226 52-53 21.983090263630643 27.48721941047569 28.34504625778883 22.184644068104838 54-55 21.806099712431244 27.24107991502785 28.430871830208048 22.521948542332858 56-57 21.93279517902349 27.54646545804772 28.28598414785528 22.234755215073513 58-59 22.122861905938358 27.06802311670544 28.42859794507597 22.380517032280228 60-61 22.078454806294765 27.257932821389986 28.7385501823187 21.925062189996552 62-63 21.909830347699803 27.403407586146017 28.7845912575665 21.902170808587684 64-65 21.840141782352323 27.273242561705263 28.65485031681225 22.231765339130163 66-67 21.84836495984609 27.49587513192438 28.29120768088731 22.36455222734222 68-69 21.6374314556331 27.38676332114767 28.58654937963886 22.38925584358037 70-71 21.942121207007485 27.40517275323865 28.475023052082776 22.177682987671083 72-73 21.959715431657774 27.313394279295323 28.338054936177226 22.388835352869677 74-75 21.611092335593174 27.998436992222608 28.0615285021738 22.328942170010414 76-77 21.88453110480856 27.479055644015414 28.27389595533833 22.3625172958377 78-79 21.864288889379427 27.428104222961203 28.288737024393683 22.418869863265687 80-81 21.768073918594336 27.91257871161794 28.043970311384243 22.27537705840348 82-83 22.072412438092567 27.6518712013807 28.018468798307705 22.257247562219025 84-85 21.81184747393141 27.567296703524956 27.91655109235574 22.704304730187893 86-87 21.761973505875755 27.59795954046386 28.078648048425432 22.56141890523495 88-89 21.896241170294626 27.42183422441841 27.939433912127498 22.74249069315946 90-91 21.7697691095586 27.348903756194648 28.255916159128354 22.625410975118395 92-93 22.03109271990327 27.251829047164446 28.22514265832196 22.491935574610324 94-95 21.702551026590434 27.183051780456267 28.171516161810246 22.942881031143052 96-97 21.7109427904451 27.204989907250553 28.145075971467698 22.93899133083665 98-99 21.70483730329591 27.222396517366647 27.979592322455154 23.09317385688229 100-101 21.830595276419135 26.53998393857891 28.63692534554555 22.992495439456402 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2271.0 1 1364.5 2 606.0 3 737.5 4 596.5 5 561.5 6 1010.0 7 2182.5 8 3473.0 9 3847.0 10 3093.0 11 2351.0 12 2238.0 13 2237.0 14 2279.5 15 2266.0 16 2224.0 17 2265.0 18 2298.5 19 2275.5 20 2353.5 21 2634.0 22 3080.5 23 3608.5 24 4248.0 25 5106.5 26 6290.0 27 7596.0 28 8387.5 29 9598.5 30 11770.0 31 13971.0 32 16777.0 33 20019.5 34 22983.5 35 25593.5 36 29136.0 37 32665.5 38 34107.0 39 34153.0 40 34003.5 41 35554.0 42 36683.0 43 38130.0 44 40410.5 45 41941.5 46 42447.5 47 43406.5 48 47568.0 49 47096.0 50 42794.5 51 39330.0 52 36046.5 53 35588.0 54 35405.0 55 33160.5 56 29966.5 57 26938.5 58 24437.5 59 21786.5 60 18712.5 61 15895.0 62 13160.5 63 10214.5 64 7909.5 65 6314.5 66 4828.5 67 3862.5 68 2962.0 69 2000.0 70 1485.5 71 1134.0 72 890.0 73 704.5 74 549.5 75 469.0 76 322.5 77 181.0 78 117.0 79 78.0 80 48.5 81 33.0 82 28.0 83 18.5 84 13.0 85 11.0 86 8.5 87 6.0 88 3.5 89 4.5 90 3.5 91 3.0 92 2.5 93 1.5 94 2.0 95 1.5 96 1.5 97 1.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33883285339929764 2 0.32121319953912514 3 0.3037662873834641 4 0.2866648586368261 5 0.2826917994330617 6 0.2281054208074293 7 0.21860462705929704 8 0.23285581768149538 9 0.23397863876082012 10-11 0.23471279100499395 12-13 0.2287100167732195 14-15 0.22655074546682583 16-17 0.22534155353524538 18-19 0.22728489771099966 20-21 0.21160858802658147 22-23 0.2027123902442395 24-25 0.2045261781416102 26-27 0.21873418333768066 28-29 0.23790851253845663 30-31 0.23134432776701983 32-33 0.23769258540781726 34-35 0.2275440102677669 36-37 0.20836968106699097 38-39 0.20919020416342057 40-41 0.20310105907939036 42-43 0.21882055418993643 44-45 0.21493386583842777 46-47 0.21510660754293928 48-49 0.22732808313712755 50-51 0.238124439669096 52-53 0.2607967883862297 54-55 0.22421873245592064 56-57 0.18841801419591328 58-59 0.18859075590042476 60-61 0.1957595366376518 62-63 0.20521714495965615 64-65 0.19208877541678254 66-67 0.19921437072788173 68-69 0.20228053598296075 70-71 0.19541405322862884 72-73 0.198091549648557 74-75 0.2015463837387869 76-77 0.21890692504219217 78-79 0.22469377214332725 80-81 0.21359511762846367 82-83 0.21109036291304703 84-85 0.20180549629555414 86-87 0.2048716615506332 88-89 0.21290415081041772 90-91 0.21303370708880132 92-93 0.20858560819763033 94-95 0.20901746245890906 96-97 0.19567316578539606 98-99 0.20301468822713464 100-101 0.19295248393934003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1157798.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.29482658274831 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5437285267571 37.99458725667465 2 12.984826636169403 13.061392077463823 3 4.518688478895873 6.817999602825921 4 2.1772057140549723 4.380087352934541 5 1.1925627589425194 2.998986857502894 6 0.7340051704907691 2.2149997656404303 7 0.46972514331074877 1.6537321237059495 8 0.3545285430597811 1.4264761273460884 9 0.26188207531602276 1.1854182206834634 >10 1.5581477594570152 15.034260667936664 >50 0.13602842026267575 4.731077491010941 >100 0.06488441646472741 5.459402684005036 >500 0.002237392665521719 0.6975083899183065 >1k 0.001204749896819387 1.1225228096873419 >5k 3.442142562341106E-4 1.2215485726640782 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7955 0.6870801296944717 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6157 0.5317853373386376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4247 0.36681700953015983 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1729 0.1493352035501875 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1677 0.14484391923288864 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1488 0.12851982815655236 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1475 0.12739700707722765 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1200 0.10364502270689706 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 8.637085225574754E-5 0.0 0.0 0.0 0.0 14-15 8.637085225574754E-5 0.0 0.0 0.0 0.0 16-17 8.637085225574754E-5 0.0 0.0 0.0 0.0 18-19 8.637085225574754E-5 0.0 0.0 0.0 0.0 20-21 8.637085225574754E-5 0.0 0.0 8.637085225574754E-5 0.0 22-23 8.637085225574754E-5 0.0 0.0 2.5911255676724264E-4 0.0 24-25 8.637085225574754E-5 0.0 0.0 6.045959657902328E-4 0.0 26-27 8.637085225574754E-5 0.0 0.0 9.068939486853492E-4 0.0 28-29 8.637085225574754E-5 0.0 0.0 0.0019001587496264459 0.0 30-31 8.637085225574754E-5 0.0 0.0 0.004275357186659503 0.0 32-33 1.2955627838362132E-4 0.0 0.0 0.007859747555273026 0.0 34-35 1.7274170451149509E-4 0.0 0.0 0.010364502270689704 0.0 36-37 1.7274170451149509E-4 0.0 0.0 0.012264661020316151 0.0 38-39 1.7274170451149509E-4 0.0 0.0 0.014121634343814724 0.0 40-41 1.7274170451149509E-4 0.0 0.0 0.017187799598893765 0.0 42-43 1.7274170451149509E-4 0.0 0.0 0.01999485229720556 0.0 44-45 1.7274170451149509E-4 0.0 0.0 0.023406500961307586 0.0 46-47 1.7274170451149509E-4 0.0 0.0 0.027336374738944097 0.0 48-49 1.7274170451149509E-4 0.0 0.0 0.033209592692334935 0.0 50-51 1.7274170451149509E-4 0.0 0.0 0.03942829405474876 0.0 52-53 1.7274170451149509E-4 0.0 0.0 0.057220689619432746 0.0 54-55 1.7274170451149509E-4 0.0 0.0 0.07954755492754349 0.0 56-57 1.7274170451149509E-4 0.0 0.0 0.10269494333208384 0.0 58-59 1.7274170451149509E-4 0.0 0.0 0.13396119184866445 0.0 60-61 1.7274170451149509E-4 0.0 0.0 0.18016959780548938 0.0 62-63 1.7274170451149509E-4 0.0 0.0 0.2104857669472568 0.0 64-65 1.7274170451149509E-4 0.0 0.0 0.24166564461158163 0.0 66-67 1.7274170451149509E-4 0.0 0.0 0.2956906126975517 0.0 68-69 1.7274170451149509E-4 0.0 0.0 0.3641395131102316 0.0 70-71 1.7274170451149509E-4 0.0 0.0 0.4242104408541041 0.0 72-73 2.5911255676724264E-4 0.0 0.0 0.48540418967730126 0.0 74-75 2.5911255676724264E-4 0.0 0.0 0.5934109404231136 0.0 76-77 2.5911255676724264E-4 0.0 0.0 0.6684672110333582 0.0 78-79 2.5911255676724264E-4 0.0 0.0 0.7449917861319505 0.0 80-81 2.5911255676724264E-4 0.0 0.0 0.8398269819087614 0.0 82-83 2.5911255676724264E-4 0.0 0.0 0.9874779538399616 0.0 84-85 2.5911255676724264E-4 0.0 0.0 1.1308967540106305 0.0 86-87 2.5911255676724264E-4 0.0 0.0 1.324021979654482 0.0 88-89 2.5911255676724264E-4 0.0 0.0 1.570049352304979 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8465 0.0 30.896025 1 GGTATCA 5880 0.0 30.08378 1 GTACATG 6840 0.0 28.572784 1 TACATGG 7095 0.0 26.668795 2 ACATGGG 7150 0.0 25.397585 3 ATCAACG 10205 0.0 25.34084 3 TATCAAC 10235 0.0 25.3152 2 TCAACGC 10270 0.0 25.226744 4 CATGGGG 2695 0.0 24.871126 4 CAACGCA 10375 0.0 24.833977 5 AACGCAG 10600 0.0 24.379637 6 TAGGGCG 100 0.0016460994 23.76868 5 GAGTACT 5595 0.0 23.558123 12-13 GTATAGG 390 0.0 23.166254 1 GTACTTT 5745 0.0 22.486841 14-15 ACGCAGA 11700 0.0 21.884525 7 CGCAGAG 11715 0.0 21.775402 8 CATGGGA 4375 0.0 21.622711 4 AGTACTT 5820 0.0 21.341576 12-13 GGACCGA 245 7.510607E-9 21.328524 6 >>END_MODULE