FastQCFastQC Report
Thu 26 May 2016
SRR937757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937757_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157798
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81110.7005539826463684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68570.592244933917661No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47780.4126799320779618No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21830.1885475704742969No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17290.1493352035501875No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17040.14717593224379383No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13620.11763710077232817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70500.029.59211
GTATCAA97000.027.827681
GCGCAAT700.00838625527.1463999
GTACATG68350.024.2653921
GAGTACT65650.023.73500612-13
TACATGG71350.022.700512
TCAACGC118900.022.5705344
ATCAACG118500.022.5665573
GTACTTT69300.022.4800414-15
TATCAAC119850.022.3916282
CAACGCA120600.022.2129925
AACGCAG122100.021.9780586
ACATGGG70500.021.8288653
AGTACTT67700.021.71812212-13
TATAGGG2509.493306E-920.8991172
GACCGAC1150.003720480320.654877
CATGGGG25250.020.1278614
ACTTTTT78800.019.95156716-17
AACCGAT1200.004759924519.794257
ACGCAGA135550.019.7665047
Produced by FastQC (version 0.11.5)