##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937757_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1157798 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.224666133470606 33.0 31.0 34.0 31.0 34.0 2 32.432184197934355 34.0 31.0 34.0 31.0 34.0 3 32.48861373054712 34.0 31.0 34.0 31.0 34.0 4 36.104569190826034 37.0 35.0 37.0 35.0 37.0 5 36.0261746867761 37.0 35.0 37.0 35.0 37.0 6 36.076695589386055 37.0 35.0 37.0 35.0 37.0 7 36.02785028130987 37.0 35.0 37.0 35.0 37.0 8 35.99562790745881 37.0 35.0 37.0 35.0 37.0 9 37.67202309902073 39.0 37.0 39.0 35.0 39.0 10-11 37.59271133651984 39.0 37.0 39.0 35.0 39.0 12-13 37.4832349857229 39.0 37.0 39.0 35.0 39.0 14-15 38.673844660294804 40.0 38.0 41.0 34.0 41.0 16-17 38.638854100628954 40.0 38.0 41.0 34.5 41.0 18-19 38.63281375507645 40.0 38.0 41.0 34.0 41.0 20-21 38.53232645072802 40.0 38.0 41.0 34.0 41.0 22-23 38.4756710583366 40.0 38.0 41.0 34.0 41.0 24-25 38.48519992261171 40.0 38.0 41.0 34.0 41.0 26-27 38.294414051501214 40.0 38.0 41.0 34.0 41.0 28-29 38.25388366537168 40.0 38.0 41.0 34.0 41.0 30-31 38.24856106160142 40.0 38.0 41.0 34.0 41.0 32-33 38.10547392550341 40.0 37.0 41.0 34.0 41.0 34-35 37.94997054753938 40.0 37.0 41.0 33.0 41.0 36-37 37.76697748657365 40.0 37.0 41.0 33.0 41.0 38-39 37.61881908588545 40.0 37.0 41.0 33.0 41.0 40-41 37.434720478010846 40.0 36.0 41.0 33.0 41.0 42-43 37.18511260168009 40.0 36.0 41.0 32.0 41.0 44-45 36.84433165370816 39.0 36.0 41.0 31.0 41.0 46-47 36.44491396599407 39.0 35.5 41.0 30.5 41.0 48-49 36.065330912646246 39.0 35.0 41.0 29.5 41.0 50-51 35.80195854544575 39.0 35.0 40.0 29.0 41.0 52-53 35.536008008305416 39.0 35.0 40.0 28.0 41.0 54-55 35.29391482797517 38.0 35.0 40.0 27.5 41.0 56-57 35.012804046992656 38.0 34.0 40.0 27.0 41.0 58-59 34.74419587872841 38.0 34.0 40.0 26.0 41.0 60-61 34.37983266511084 37.5 34.0 40.0 26.0 41.0 62-63 33.965574737562164 37.0 33.0 40.0 25.5 41.0 64-65 33.5625052902147 36.0 33.0 39.0 24.0 41.0 66-67 33.12078790946262 36.0 32.5 39.0 22.5 40.0 68-69 32.641027191271704 35.0 32.0 38.5 22.0 40.0 70-71 32.203338578923095 35.0 31.5 38.0 20.5 40.0 72-73 31.720400708931955 35.0 31.0 37.0 19.5 39.0 74-75 31.2281023978276 35.0 31.0 36.5 18.0 39.0 76-77 29.981957128963774 33.5 29.5 35.0 17.5 37.5 78-79 30.598454566340585 34.0 30.5 36.0 17.5 38.0 80-81 30.42590503697536 34.0 30.0 35.0 14.0 37.0 82-83 30.03071563433345 34.0 30.0 35.0 8.5 37.0 84-85 29.620624236697594 34.0 30.0 35.0 7.0 36.0 86-87 29.265388262892145 34.0 29.0 35.0 2.0 36.0 88-89 28.84261719229088 34.0 29.0 35.0 2.0 35.5 90-91 28.320633651120488 33.5 29.0 35.0 2.0 35.0 92-93 27.836673150238642 33.0 27.0 35.0 2.0 35.0 94-95 27.3011026102999 33.0 26.5 35.0 2.0 35.0 96-97 26.731962743069168 33.0 25.5 35.0 2.0 35.0 98-99 25.8693895653646 32.0 24.5 34.0 2.0 35.0 100-101 23.409108065482926 30.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21916026154892165 1101 2 0.21899879591173033 1101 3 0.24721018680982354 1101 4 0.2152662343660765 1101 5 0.211614057869852 1101 6 0.1867627072499829 1101 7 0.2109605337783762 1101 8 0.13123368647020328 1101 9 0.161062354671607 1101 10-11 0.21007324876071465 1101 12-13 0.19726508921868202 1101 14-15 0.2939397173959222 1101 16-17 0.288809627231295 1101 18-19 0.2817837897857487 1101 20-21 0.217386193295674 1101 22-23 0.2885681859193383 1101 24-25 0.2324103485469422 1101 26-27 0.25853900713097744 1101 28-29 0.2707834883240281 1101 30-31 0.26762663037591494 1101 32-33 0.3125051270945818 1101 34-35 0.3768649129896886 1101 36-37 0.2948416482056615 1101 38-39 0.35630067798063436 1101 40-41 0.2744540655687828 1101 42-43 0.2807493060134618 1101 44-45 0.4927150141479544 1101 46-47 0.5458230673553786 1101 48-49 0.43659077818752223 1101 50-51 0.4903434872770731 1101 52-53 0.5671235331997835 1101 54-55 0.6937399393031569 1101 56-57 0.7509403857739372 1101 58-59 0.7028627474926452 1101 60-61 0.8331994102111935 1101 62-63 0.8524532906669151 1101 64-65 0.7367237048856126 1101 66-67 0.8170897953767167 1101 68-69 0.8082950366564461 1101 70-71 0.6596867028408155 1101 72-73 0.7527093692349887 1101 74-75 0.6577622053939329 1101 76-77 0.6425090916796599 1101 78-79 0.7568534048589264 1101 80-81 0.6698766171778558 1101 82-83 0.8929684537218492 1101 84-85 1.0998814261608096 1101 86-87 1.0735483227284845 1101 88-89 1.200027120622515 1101 90-91 1.272762241099091 1101 92-93 1.2103968114609138 1101 94-95 1.1937682319586465 1101 96-97 1.5038463907516295 1101 98-99 1.5239217724548055 1101 100-101 1.7307754898977485 1102 1 0.10445326668577337 1102 2 0.11237914281798567 1102 3 0.08026073582082205 1102 4 0.02503082592841821 1102 5 -0.020303687217740674 1102 6 0.11137776912920572 1102 7 0.025042975643643217 1102 8 0.0660322447592776 1102 9 0.13805954385703956 1102 10-11 0.19615748841689395 1102 12-13 0.14583403026113473 1102 14-15 0.03783467379894745 1102 16-17 0.16848616616857015 1102 18-19 0.07832662916498379 1102 20-21 0.06869970020532179 1102 22-23 0.06829885423235282 1102 24-25 0.11158336472705344 1102 26-27 0.1380717988807092 1102 28-29 0.1419466068717128 1102 30-31 0.08704266354048684 1102 32-33 0.20442517648492498 1102 34-35 0.15392742698397655 1102 36-37 0.14877575601659743 1102 38-39 0.15741299317446789 1102 40-41 0.20403451942947015 1102 42-43 -0.04651086715706754 1102 44-45 -0.05055071543559109 1102 46-47 -0.2148109637179303 1102 48-49 0.09387384221453487 1102 50-51 0.056478878106382524 1102 52-53 0.0342298832929302 1102 54-55 0.053992401754975106 1102 56-57 0.02750290955064827 1102 58-59 0.054798524836130014 1102 60-61 0.12699909673906262 1102 62-63 0.022514637292744055 1102 64-65 0.24754360188763513 1102 66-67 0.4205934768264328 1102 68-69 0.24229306712975784 1102 70-71 0.20505320653965953 1102 72-73 0.38230846403530805 1102 74-75 0.32644232904368664 1102 76-77 0.14543294751726066 1102 78-79 0.23914967514928875 1102 80-81 0.22169679895691274 1102 82-83 0.36098429689013756 1102 84-85 0.3436443199302772 1102 86-87 0.2757248018843228 1102 88-89 0.3875636688204871 1102 90-91 0.3933261865871174 1102 92-93 0.44704136624136126 1102 94-95 0.4423389120975152 1102 96-97 0.7993726739330427 1102 98-99 0.774155191800947 1102 100-101 1.148638781702907 1103 1 0.055335205952182775 1103 2 0.10854660910668912 1103 3 0.06114643588320945 1103 4 0.02846119359513466 1103 5 -3.948376082760774E-4 1103 6 0.06667375246865959 1103 7 0.01600130471981487 1103 8 0.045244064528020544 1103 9 0.09063981946034261 1103 10-11 0.08145946076961508 1103 12-13 0.14908124933727862 1103 14-15 0.2083207403759033 1103 16-17 0.14203423805412996 1103 18-19 0.23100543544920527 1103 20-21 0.15400591160553034 1103 22-23 0.23185951282815864 1103 24-25 0.05709530036310184 1103 26-27 0.017990452646181154 1103 28-29 0.1392045409556033 1103 30-31 0.2235198846383284 1103 32-33 0.18982165785811134 1103 34-35 0.1685624667331993 1103 36-37 0.025382789792026017 1103 38-39 0.09907310199842811 1103 40-41 0.244431826873452 1103 42-43 0.4297485647236172 1103 44-45 0.35186994372542557 1103 46-47 0.31491128084685016 1103 48-49 0.257569280782036 1103 50-51 0.21754882456395563 1103 52-53 0.3084133775289146 1103 54-55 0.31308760424014537 1103 56-57 0.34248968154858517 1103 58-59 0.15764406698856703 1103 60-61 0.37026390242764506 1103 62-63 0.3031575160190272 1103 64-65 0.28194238538969074 1103 66-67 0.254080158608744 1103 68-69 0.4309785058929094 1103 70-71 0.37243688816327847 1103 72-73 0.3338806071890268 1103 74-75 0.36235820242877637 1103 76-77 0.3663786246670533 1103 78-79 0.4436584456298682 1103 80-81 0.4256216134714066 1103 82-83 0.47006259753208113 1103 84-85 0.6258265707123272 1103 86-87 0.7933407615720682 1103 88-89 0.7129996928982756 1103 90-91 0.740516132870777 1103 92-93 1.0743701250265154 1103 94-95 0.9747170829593905 1103 96-97 1.1773082143246434 1103 98-99 1.413098940727597 1103 100-101 2.1614648894529758 1104 1 0.16675074537561585 1104 2 0.07143457060283964 1104 3 0.16380211215351181 1104 4 0.07104537753875917 1104 5 0.07596179844866668 1104 6 0.1343829075062999 1104 7 0.034934992777266416 1104 8 0.09408598825081071 1104 9 0.03279976084873226 1104 10-11 0.11505064266225418 1104 12-13 0.09954909399924361 1104 14-15 0.1383504351935727 1104 16-17 0.07205609854035089 1104 18-19 0.14714540091330974 1104 20-21 0.08302794930973079 1104 22-23 0.14640154482680856 1104 24-25 0.01369877729342761 1104 26-27 0.03605128812089475 1104 28-29 -0.039244239806301096 1104 30-31 0.2284730731571969 1104 32-33 0.1739636202662922 1104 34-35 -0.012007526804829638 1104 36-37 0.029444215272938834 1104 38-39 -0.04049254105429867 1104 40-41 0.1544248137960622 1104 42-43 0.16955853085769945 1104 44-45 0.19320906329785714 1104 46-47 0.29401982037618524 1104 48-49 0.2639126579083779 1104 50-51 0.2887788362127921 1104 52-53 0.330561842972493 1104 54-55 0.24120545203583532 1104 56-57 0.1960968238654317 1104 58-59 0.2845891423818472 1104 60-61 0.37003535606893223 1104 62-63 0.3657994795077997 1104 64-65 0.485457514834458 1104 66-67 0.4242869525409745 1104 68-69 0.33144305473267366 1104 70-71 0.504377565524976 1104 72-73 0.5657211671978466 1104 74-75 0.8366656475290206 1104 76-77 0.7576318907728208 1104 78-79 0.8143411887048622 1104 80-81 0.8220244666808938 1104 82-83 0.5761848303921866 1104 84-85 0.8099071551858721 1104 86-87 0.7519341586894654 1104 88-89 0.5164943990514139 1104 90-91 0.4082497094105726 1104 92-93 0.5323119218871675 1104 94-95 0.7523279684657673 1104 96-97 0.9142327151426564 1104 98-99 1.0972124834230854 1104 100-101 1.5920343426922834 1105 1 0.08394227995954395 1105 2 9.269529837254709E-4 1105 3 0.02629645764862687 1105 4 0.0650045265817667 1105 5 -0.011515285926286367 1105 6 0.03420888944218348 1105 7 -0.017340228478907704 1105 8 -0.01631050887810659 1105 9 0.023460358527195524 1105 10-11 0.038282216551756676 1105 12-13 -0.002546644073269988 1105 14-15 -0.0910563450177122 1105 16-17 0.1561564424641162 1105 18-19 0.17770218296434592 1105 20-21 0.19474906155355143 1105 22-23 0.0421140773740305 1105 24-25 0.16369547657765793 1105 26-27 0.08824128597813541 1105 28-29 0.11230623084330205 1105 30-31 0.10910809840497393 1105 32-33 0.20247504074284706 1105 34-35 0.16054090714082037 1105 36-37 0.01614665953413663 1105 38-39 0.09490823077208432 1105 40-41 0.08321944650756308 1105 42-43 -0.1339368730308763 1105 44-45 0.033622161403783934 1105 46-47 -0.07862584940938433 1105 48-49 -0.07427624476054007 1105 50-51 -0.016383429138777217 1105 52-53 -0.03640361963634575 1105 54-55 0.05329110398671588 1105 56-57 -0.01530374017819014 1105 58-59 0.04156203971768235 1105 60-61 0.12518230826374577 1105 62-63 0.09709930137736933 1105 64-65 0.06226111250642674 1105 66-67 -0.11366289173017208 1105 68-69 0.015647344148092657 1105 70-71 0.051755011571799514 1105 72-73 0.0020265515851356497 1105 74-75 -0.00878861753685456 1105 76-77 -0.03667805401324031 1105 78-79 0.07134465824412572 1105 80-81 0.01479790122174407 1105 82-83 8.008110510715483E-4 1105 84-85 0.3473456590634001 1105 86-87 0.34164842492412006 1105 88-89 0.32551092643869595 1105 90-91 0.4053829566006364 1105 92-93 0.38933438406961685 1105 94-95 0.5767872462908024 1105 96-97 0.5486770910476473 1105 98-99 0.530002605517101 1105 100-101 0.4269184713357852 1106 1 -0.25847749481381754 1106 2 -0.18191546156294436 1106 3 -0.16614126694192777 1106 4 -0.1820651221943379 1106 5 -0.047549108261435435 1106 6 -0.2122159600756106 1106 7 -0.1818407181778099 1106 8 -0.12920614550409937 1106 9 -0.4715049791559167 1106 10-11 -0.41143651155557137 1106 12-13 -0.6806280003126588 1106 14-15 -0.499631652342984 1106 16-17 -0.4048729359842511 1106 18-19 -0.388424595026045 1106 20-21 -0.31428335616482883 1106 22-23 -0.4585490202416054 1106 24-25 -0.37896845056351225 1106 26-27 -0.19774548343816178 1106 28-29 -0.3242045686729256 1106 30-31 -0.4243674393053283 1106 32-33 -0.5235462690012866 1106 34-35 -0.7931581493610267 1106 36-37 -0.6734888023338286 1106 38-39 -0.52272493770176 1106 40-41 -0.5338813269030069 1106 42-43 -0.8979839120081152 1106 44-45 -0.9905647815855048 1106 46-47 -0.635092837430058 1106 48-49 -0.7601187185590419 1106 50-51 -0.9066995898994321 1106 52-53 -0.954991613822699 1106 54-55 -1.165847409350171 1106 56-57 -1.1202605037738707 1106 58-59 -0.9443656521449526 1106 60-61 -1.3638395486884036 1106 62-63 -1.3192170824489722 1106 64-65 -1.678213285477618 1106 66-67 -1.8280176916507074 1106 68-69 -1.6466921966851302 1106 70-71 -1.5289921539255147 1106 72-73 -1.6616304709758687 1106 74-75 -1.6522616806121597 1106 76-77 -1.5974834954219013 1106 78-79 -1.4618476906027311 1106 80-81 -1.4503358859108495 1106 82-83 -1.660527963377234 1106 84-85 -1.563367070668118 1106 86-87 -1.5290379551728464 1106 88-89 -1.5942237648070225 1106 90-91 -1.8806229825233416 1106 92-93 -1.852571642537935 1106 94-95 -1.813356387100736 1106 96-97 -2.0378305716073193 1106 98-99 -2.210799420198189 1106 100-101 -1.8237916791987026 1107 1 0.12411366414919911 1107 2 0.15774451523394362 1107 3 0.07995088773104442 1107 4 0.06741172348326785 1107 5 0.09315320156880347 1107 6 0.06878748424956882 1107 7 0.03926833284374709 1107 8 0.037661108830221224 1107 9 0.04347404045829961 1107 10-11 0.027961617015705542 1107 12-13 0.1825151903685054 1107 14-15 0.0539532649100849 1107 16-17 0.07780424075335901 1107 18-19 0.1632710032492639 1107 20-21 0.09128502977306852 1107 22-23 0.10932099172137555 1107 24-25 0.1269651494972095 1107 26-27 0.06317660542035952 1107 28-29 0.11318406902960731 1107 30-31 0.2048001760867919 1107 32-33 0.27804542124798104 1107 34-35 0.27931995766966367 1107 36-37 0.3429142090111199 1107 38-39 0.33829401150799043 1107 40-41 0.198760611460564 1107 42-43 0.3311287676624133 1107 44-45 0.43501538661899986 1107 46-47 0.31578667624423673 1107 48-49 0.22646991769781266 1107 50-51 0.29979005363279043 1107 52-53 0.2116371038257583 1107 54-55 0.21097278820158039 1107 56-57 0.33810671261142744 1107 58-59 0.3315761159901598 1107 60-61 0.39326414610029303 1107 62-63 0.37435814876897666 1107 64-65 0.319073359720754 1107 66-67 0.30007921929920656 1107 68-69 0.4176783736705403 1107 70-71 0.3547600642849531 1107 72-73 0.28062015879712376 1107 74-75 0.3497568457371081 1107 76-77 0.27713023778565926 1107 78-79 0.5320596848793109 1107 80-81 0.65888680977055 1107 82-83 0.5402006577722958 1107 84-85 0.6740600581814498 1107 86-87 0.7985451983544252 1107 88-89 0.7332581913383684 1107 90-91 0.8940113515798167 1107 92-93 1.0427468942924918 1107 94-95 1.3143727797918174 1107 96-97 1.1148347144379223 1107 98-99 1.0560788574223707 1107 100-101 1.644643773135524 1108 1 0.13224473517933433 1108 2 0.13965926307723464 1108 3 0.14681609696821596 1108 4 0.15487631251014733 1108 5 0.02207660800885236 1108 6 0.04100269827975467 1108 7 0.07465607601575641 1108 8 0.09697805416808336 1108 9 0.17710683432747487 1108 10-11 0.15579486242808827 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0.7955373550293992 2316 92-93 0.8351821658256107 2316 94-95 0.9280710806594108 2316 96-97 1.1155736794970537 2316 98-99 0.9295355573181041 2316 100-101 0.9463396573437599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 12.0 5 23.0 6 56.0 7 118.0 8 309.0 9 567.0 10 978.0 11 1574.0 12 2534.0 13 3653.0 14 4711.0 15 6566.0 16 9457.0 17 13443.0 18 12168.0 19 7154.0 20 6300.0 21 6391.0 22 6812.0 23 7381.0 24 8440.0 25 10003.0 26 12210.0 27 14845.0 28 18432.0 29 23182.0 30 28590.0 31 36004.0 32 45029.0 33 59455.0 34 81743.0 35 117336.0 36 173794.0 37 248289.0 38 177550.0 39 12681.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.135816549590885 19.895366218236173 15.375981751730217 21.59283548044273 2 15.70351650287874 20.98638968110154 39.98305403878742 23.327039777232297 3 19.43914223379208 25.529582880606117 29.960839455587244 25.070435430014566 4 13.043466995106229 17.40908172237299 36.656998889270845 32.890452393249944 5 13.41058487159775 38.366488438182934 33.43468458741114 14.788242102808177 6 31.5418579061287 36.62365974030013 16.941383557408116 14.893098796163063 7 28.585729116823487 33.79207771994769 19.986387953684495 17.635805209544326 8 26.895278796474 35.115624659914765 18.96868020155502 19.020416342056212 9 27.07398008979114 17.047965189091705 20.42722478359783 35.45082993751932 10-11 25.405770263897505 27.205004672663108 26.208155481353394 21.181069582085996 12-13 26.298248099271493 24.72661641821728 27.355547224962294 21.619588257548926 14-15 23.155291337521746 25.81512491816362 26.305495431845628 24.724088312469014 16-17 21.744077982515083 29.331325498921228 26.8164222083645 22.10817431019919 18-19 21.628211484214 27.28291118139779 30.073769344911632 21.015107989476576 20-21 22.523552363657565 26.165353463595594 30.266779237212194 21.04431493553465 22-23 22.271032214739016 26.082559040921076 30.35756006659311 21.288848677746806 24-25 22.13634076770765 26.41113838991706 30.43697192298308 21.015548919392206 26-27 21.88936748723202 26.54293263222083 30.4215225876441 21.146177292903047 28-29 21.47537273856391 26.718155922272068 30.6430744302468 21.163396908917225 30-31 21.720757852406035 26.455953456475136 30.531578047293223 21.291710643825606 32-33 21.555573597466914 26.659486369815806 30.357886263406915 21.427053769310366 34-35 22.069696095519255 26.57130172966269 30.11712751274402 21.24187466207404 36-37 22.20117162617654 26.170362996823698 30.126641863719104 21.50182351328066 38-39 21.829153271986996 26.709711020402523 30.225091078063706 21.236044629546775 40-41 21.744432856350095 26.741722969690297 30.279647347657946 21.234196826301662 42-43 21.70385053824426 26.697442405639332 29.787514357283957 21.811192698832453 44-45 21.852559772948304 26.837885365150054 29.610044239150525 21.69951062275112 46-47 22.172347853425208 27.224179001863885 28.98230088495575 21.621172259755156 48-49 22.29015769590205 27.291073226935957 28.678793710129057 21.739975367032937 50-51 22.162328834563542 27.398734494272748 28.54733727299581 21.8915993981679 52-53 22.42191643101819 27.789821713286774 28.08533958427981 21.702922271415222 54-55 22.193335970523357 27.513650913199022 28.305498886679715 21.98751422959791 56-57 22.34288710120418 27.741454036023555 28.13353451983852 21.78212434293374 58-59 22.486824126488386 27.32920595820687 28.21563001490761 21.968339900397133 60-61 22.41042910766818 27.49231731269185 28.609394730341563 21.487858849298412 62-63 22.207975829980704 27.57821312526019 28.711614633986237 21.502196410772864 64-65 22.15524642467857 27.555627147395313 28.469344393408868 21.819782034517246 66-67 22.22861846366983 27.732341911110574 28.151024617420312 21.88801500779929 68-69 22.068789201570567 27.57899046293049 28.388414904845234 21.963805430653707 70-71 22.29136688783363 27.65180109138209 28.28291290881484 21.773919111969445 72-73 22.407362942413098 27.585684203980314 28.04677499874762 21.960177854858966 74-75 22.14889816703777 28.158797994123326 27.782696117975675 21.909607720863225 76-77 22.24299921057041 27.753761882470002 28.15279521989155 21.85044368706804 78-79 22.194501977028807 27.686003948875364 28.138371287564844 21.981122786530985 80-81 22.167467900272758 28.20954086982358 27.828084000836068 21.794907229067594 82-83 22.39226738366052 28.01453968182678 27.733877355011284 21.859315579501416 84-85 22.167103056716886 27.86763972362971 27.72961868449021 22.23563853516319 86-87 22.126225818320638 27.84799248228102 27.886341140682568 22.13944055871577 88-89 22.19687717546584 27.78075277379992 27.788569335929065 22.233800714805174 90-91 22.17764924174104 27.755448096664626 28.03852107541817 22.028381586176156 92-93 22.275879313866778 27.734247970332454 27.93161124875676 22.058261467044005 94-95 22.08442232582886 27.590607342558894 27.982385528390964 22.34258480322129 96-97 22.191781295182754 27.541678254756015 27.990072534241722 22.276467915819513 98-99 22.120342651628874 27.652630778877764 27.65776986234166 22.569256707151705 100-101 21.86717955672961 27.571454610006885 28.14763528368044 22.413730549583068 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1309.0 1 865.5 2 629.0 3 822.0 4 642.5 5 562.0 6 1030.5 7 2198.5 8 3349.0 9 3681.0 10 3067.5 11 2366.0 12 2280.0 13 2303.0 14 2353.0 15 2404.0 16 2389.5 17 2404.5 18 2410.5 19 2437.0 20 2537.5 21 2809.0 22 3216.5 23 3702.5 24 4287.0 25 5151.0 26 6439.0 27 7715.0 28 8333.0 29 9466.0 30 11597.0 31 13703.0 32 16680.0 33 19995.5 34 22933.0 35 25530.0 36 29496.0 37 32821.0 38 33616.0 39 34103.0 40 34159.0 41 35496.0 42 36725.0 43 38354.5 44 40812.0 45 42440.0 46 42720.0 47 43731.0 48 48195.5 49 47261.5 50 43128.0 51 39895.0 52 36297.5 53 35673.0 54 35278.0 55 32834.0 56 29415.0 57 26687.5 58 24534.5 59 21922.0 60 18676.5 61 15552.0 62 12796.0 63 9993.0 64 7659.0 65 6008.0 66 4514.0 67 3749.5 68 2898.0 69 1852.5 70 1284.5 71 998.0 72 868.5 73 717.5 74 543.0 75 408.5 76 274.0 77 164.0 78 104.5 79 78.0 80 45.5 81 28.5 82 19.5 83 8.5 84 5.5 85 3.0 86 2.0 87 1.5 88 0.5 89 2.0 90 2.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03696672476545995 2 0.0 3 0.0 4 0.0 5 0.009328052043620735 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007255151589482795 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.7503968740661147E-4 22-23 0.0017706024712428247 24-25 4.318542612787377E-5 26-27 0.0016410461928592034 28-29 0.0013819336360919607 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.7503968740661147E-4 38-39 0.0 40-41 4.318542612787377E-5 42-43 0.024572507466760176 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.637085225574754E-5 84-85 3.022979828951164E-4 86-87 0.0 88-89 0.0 90-91 0.012610144429339143 92-93 0.0029797944028232904 94-95 0.0 96-97 0.0 98-99 3.4548340902299017E-4 100-101 0.01511489914475582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1157798.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.080563988995905 #Duplication Level Percentage of deduplicated Percentage of total 1 73.66706430167895 35.419539990383505 2 13.908818631539285 13.37487688450126 3 4.909079893010602 7.080939897689681 4 2.2840961566819695 4.392825256734682 5 1.278903273688419 3.074519534315619 6 0.8052541518829006 2.3230244266206257 7 0.5189370358692225 1.746554975155901 8 0.39060198448009037 1.5024290967219014 9 0.29579442134491707 1.2799766342756072 >10 1.7141436759865922 15.690711111683363 >50 0.14987638713756785 4.941075759518445 >100 0.07328847877971409 5.954100146791923 >500 0.0028811185530016304 0.9056990284722307 >1k 9.003495478130094E-4 1.0178183518515957 >5k 3.601398191252038E-4 1.2959089052836583 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8111 0.7005539826463684 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6857 0.592244933917661 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4778 0.4126799320779618 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2183 0.1885475704742969 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1729 0.1493352035501875 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1704 0.14717593224379383 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1362 0.11763710077232817 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.318542612787377E-5 0.0 12-13 0.0 0.0 0.0 8.637085225574754E-5 0.0 14-15 0.0 0.0 0.0 8.637085225574754E-5 0.0 16-17 0.0 0.0 0.0 8.637085225574754E-5 0.0 18-19 0.0 0.0 0.0 8.637085225574754E-5 0.0 20-21 0.0 0.0 0.0 1.7274170451149509E-4 0.0 22-23 0.0 0.0 0.0 3.454834090229902E-4 0.0 24-25 0.0 0.0 0.0 6.909668180459803E-4 0.0 26-27 0.0 0.0 0.0 9.932648009410969E-4 0.0 28-29 0.0 0.0 0.0 0.002072900454137941 0.0 30-31 0.0 0.0 0.0 0.004448098891170999 0.0 32-33 0.0 0.0 0.0 0.0079029329814009 0.0 34-35 0.0 0.0 0.0 0.010450873122945454 0.0 36-37 0.0 0.0 0.0 0.012351031872571899 0.0 38-39 0.0 0.0 0.0 0.014208005196070472 0.0 40-41 0.0 0.0 0.0 0.017317355877277382 0.0 42-43 0.0 0.0 0.0 0.020210779427844924 0.0 44-45 0.0 0.0 0.0 0.02357924266581908 0.0 46-47 0.0 0.0 0.0 0.027638672721839216 0.0 48-49 0.0 0.0 0.0 0.033641446953613666 0.0 50-51 0.0 0.0 0.0 0.04007607544666686 0.0 52-53 0.0 0.0 0.0 0.05825713984650172 0.0 54-55 0.0 0.0 0.0 0.08079993228525184 0.0 56-57 0.0 0.0 0.0 0.1039041352636643 0.0 58-59 0.0 0.0 0.0 0.13495445664960554 0.0 60-61 0.0 0.0 0.0 0.1811196771803026 0.0 62-63 0.0 0.0 0.0 0.21152221717432573 0.0 64-65 8.637085225574754E-5 0.0 0.0 0.24313394909992936 0.0 66-67 8.637085225574754E-5 0.0 0.0 0.2973748443165388 0.0 68-69 8.637085225574754E-5 0.0 0.0 0.3665147115472647 0.0 70-71 1.2955627838362132E-4 0.0 0.0 0.426974308126288 0.0 72-73 1.7274170451149509E-4 0.0 0.0 0.4880385006711015 0.0 74-75 1.7274170451149509E-4 0.0 0.0 0.5963475493998089 0.0 76-77 1.7274170451149509E-4 0.0 0.0 0.6720516014019717 0.0 78-79 1.7274170451149509E-4 0.0 0.0 0.748532991074436 0.0 80-81 1.7274170451149509E-4 0.0 0.0 0.8434113722773748 0.0 82-83 1.7274170451149509E-4 0.0 0.0 0.9936102843501198 0.0 84-85 2.1592713063936888E-4 0.0 0.0 1.1387565015659036 0.0 86-87 2.5911255676724264E-4 0.0 0.0 1.3339114422377651 0.0 88-89 2.5911255676724264E-4 0.0 0.0 1.5836959469613872 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7050 0.0 29.5921 1 GTATCAA 9700 0.0 27.82768 1 GCGCAAT 70 0.008386255 27.146399 9 GTACATG 6835 0.0 24.265392 1 GAGTACT 6565 0.0 23.735006 12-13 TACATGG 7135 0.0 22.70051 2 TCAACGC 11890 0.0 22.570534 4 ATCAACG 11850 0.0 22.566557 3 GTACTTT 6930 0.0 22.48004 14-15 TATCAAC 11985 0.0 22.391628 2 CAACGCA 12060 0.0 22.212992 5 AACGCAG 12210 0.0 21.978058 6 ACATGGG 7050 0.0 21.828865 3 AGTACTT 6770 0.0 21.718122 12-13 TATAGGG 250 9.493306E-9 20.899117 2 GACCGAC 115 0.0037204803 20.65487 7 CATGGGG 2525 0.0 20.127861 4 ACTTTTT 7880 0.0 19.951567 16-17 AACCGAT 120 0.0047599245 19.79425 7 ACGCAGA 13555 0.0 19.766504 7 >>END_MODULE